Restriction map for
pFN21AE2231
(Linear) (Six-base) MAPSORT of: KIEE2231.seq Check: 7295 from: 1 to: 1548
KIEE2231.orf
With 182 enzymes: SgfI *
July 3, 2012 17:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1548
Size: 34 1514
AflII C'TTAA_G
Cuts at: 0 982 1548
Size: 982 566
AhdI GACnn_n'nnGTC
Cuts at: 0 1084 1548
Size: 1084 464
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 936 968 1548
Size: 936 32 580
Fragments arranged by size:
936 580 32
ApoI r'AATT_y
Cuts at: 0 93 847 1548
Size: 93 754 701
Fragments arranged by size:
754 701 93
AvaI C'yCGr_G
Cuts at: 0 428 1548
Size: 428 1120
BamHI G'GATC_C
Cuts at: 0 1460 1548
Size: 1460 88
BanI G'GyrC_C
Cuts at: 0 835 1014 1548
Size: 835 179 534
Fragments arranged by size:
835 534 179
BanII G_rGCy'C
Cuts at: 0 583 761 1548
Size: 583 178 787
Fragments arranged by size:
787 583 178
BbsI GAAGACnn'nnnn_
Cuts at: 0 354 1080 1548
Size: 354 726 468
Fragments arranged by size:
726 468 354
BbvCI CC'TCA_GC
Cuts at: 0 592 1548
Size: 592 956
BclI T'GATC_A
Cuts at: 0 1443 1548
Size: 1443 105
BfrBI ATG'CAT
Cuts at: 0 612 1548
Size: 612 936
Bme1580I G_kGCm'C
Cuts at: 0 394 1548
Size: 394 1154
BmrI ACTGGGnnnn_n'
Cuts at: 0 320 1548
Size: 320 1228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 1122 1335 1548
Size: 60 1062 213 213
Fragments arranged by size:
1062 213 213 60
Bpu10I CC'TnA_GC
Cuts at: 0 592 1548
Size: 592 956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 1059 1548
Size: 250 809 489
Fragments arranged by size:
809 489 250
BseYI C'CCAG_C
Cuts at: 0 405 409 451 1417 1548
Size: 405 4 42 966 131
Fragments arranged by size:
966 405 131 42 4
BsiHKAI G_wGCw'C
Cuts at: 0 761 1548
Size: 761 787
Bsp1286I G_dGCh'C
Cuts at: 0 394 583 761 1548
Size: 394 189 178 787
Fragments arranged by size:
787 394 189 178
BspHI T'CATG_A
Cuts at: 0 1398 1548
Size: 1398 150
BsrDI GCAATG_nn'
Cuts at: 0 1185 1548
Size: 1185 363
BsrFI r'CCGG_y
Cuts at: 0 382 707 1548
Size: 382 325 841
Fragments arranged by size:
841 382 325
BstEII G'GTnAC_C
Cuts at: 0 703 1548
Size: 703 845
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1387 1548
Size: 1387 161
BstYI r'GATC_y
Cuts at: 0 1048 1460 1548
Size: 1048 412 88
Fragments arranged by size:
1048 412 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 846 1548
Size: 846 702
BtsI GCAGTG_nn'
Cuts at: 0 95 1532 1548
Size: 95 1437 16
Fragments arranged by size:
1437 95 16
DrdI GACnn_nn'nnGTC
Cuts at: 0 680 1548
Size: 680 868
EaeI y'GGCC_r
Cuts at: 0 188 384 917 1548
Size: 188 196 533 631
Fragments arranged by size:
631 533 196 188
EarI CTCTTCn'nnn_
Cuts at: 0 101 233 730 1246 1473 1548
Size: 101 132 497 516 227 75
Fragments arranged by size:
516 497 227 132 101 75
EcoICRI GAG'CTC
Cuts at: 0 759 1548
Size: 759 789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 60 1122 1335 1548
Size: 34 26 1062 213 213
Fragments arranged by size:
1062 213 213 34 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 145 1036 1548
Size: 145 891 512
Fragments arranged by size:
891 512 145
EcoO109I rG'GnC_Cy
Cuts at: 0 138 425 626 923 952 1007 1299 1335
Size: 138 287 201 297 29 55 292 36
Cuts at: 1335 1548
Size: 213
Fragments arranged by size:
297 292 287 213 201 138 55 36
29
EcoRI G'AATT_C
Cuts at: 0 847 1548
Size: 847 701
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1318 1548
Size: 1318 230
FseI GG_CCGG'CC
Cuts at: 0 386 1548
Size: 386 1162
HaeII r_GCGC'y
Cuts at: 0 501 1548
Size: 501 1047
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 142 174 245 277 1033 1065 1070 1102
Size: 142 32 71 32 756 32 5 32
Cuts at: 1102 1548
Size: 446
Fragments arranged by size:
756 446 142 71 32 32 32 32
5
HindIII A'AGCT_T
Cuts at: 0 226 1548
Size: 226 1322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 1076 1548
Size: 140 936 472
Fragments arranged by size:
936 472 140
MscI TGG'CCA
Cuts at: 0 190 919 1548
Size: 190 729 629
Fragments arranged by size:
729 629 190
MslI CAynn'nnrTG
Cuts at: 0 1439 1548
Size: 1439 109
MspA1I CmG'CkG
Cuts at: 0 12 409 551 1548
Size: 12 397 142 997
Fragments arranged by size:
997 397 142 12
NaeI GCC'GGC
Cuts at: 0 384 1548
Size: 384 1164
NgoMIV G'CCGG_C
Cuts at: 0 382 1548
Size: 382 1166
NsiI A_TGCA'T
Cuts at: 0 614 1548
Size: 614 934
NspI r_CATG'y
Cuts at: 0 374 612 1548
Size: 374 238 936
Fragments arranged by size:
936 374 238
PflMI CCAn_nnn'nTGG
Cuts at: 0 802 1548
Size: 802 746
PfoI T'CCnGG_A
Cuts at: 0 743 1548
Size: 743 805
PpuMI rG'GwC_Cy
Cuts at: 0 138 425 923 952 1007 1335 1548
Size: 138 287 498 29 55 328 213
Fragments arranged by size:
498 328 287 213 138 55 29
PstI C_TGCA'G
Cuts at: 0 556 1548
Size: 556 992
PvuII CAG'CTG
Cuts at: 0 12 409 551 1548
Size: 12 397 142 997
Fragments arranged by size:
997 397 142 12
SacI G_AGCT'C
Cuts at: 0 761 1548
Size: 761 787
SapI GCTCTTCn'nnn_
Cuts at: 0 730 1246 1548
Size: 730 516 302
Fragments arranged by size:
730 516 302
SfcI C'TryA_G
Cuts at: 0 510 552 1548
Size: 510 42 996
Fragments arranged by size:
996 510 42
SmaI CCC'GGG
Cuts at: 0 430 1548
Size: 430 1118
SmlI C'TyrA_G
Cuts at: 0 229 982 1074 1548
Size: 229 753 92 474
Fragments arranged by size:
753 474 229 92
SpeI A'CTAG_T
Cuts at: 0 346 1548
Size: 346 1202
SphI G_CATG'C
Cuts at: 0 374 612 1548
Size: 374 238 936
Fragments arranged by size:
936 374 238
StuI AGG'CCT
Cuts at: 0 284 1484 1498 1548
Size: 284 1200 14 50
Fragments arranged by size:
1200 284 50 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 115 853 873 1032 1548
Size: 115 738 20 159 516
Fragments arranged by size:
738 516 159 115 20
TatI w'GTAC_w
Cuts at: 0 817 978 1548
Size: 817 161 570
Fragments arranged by size:
817 570 161
Tth111I GACn'n_nGTC
Cuts at: 0 358 677 1548
Size: 358 319 871
Fragments arranged by size:
871 358 319
XbaI T'CTAG_A
Cuts at: 0 120 1548
Size: 120 1428
XmaI C'CCGG_G
Cuts at: 0 428 1548
Size: 428 1120
XmnI GAAnn'nnTTC
Cuts at: 0 739 1548
Size: 739 809
Enzymes that do cut:
AcuI AflII AhdI AloI ApoI AvaI BamHI BanI
BanII BbsI BbvCI BclI BfrBI Bme1580I BmrI BpmI
Bpu10I BpuEI BseYI BsiHKAI Bsp1286I BspHI BsrDI BsrFI
BstEII BstXI BstYI BtgZI BtsI DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII
Hin4I HindIII MmeI MscI MslI MspA1I NaeI NgoMIV
NsiI NspI PflMI PfoI PpuMI PstI PvuII SacI
SapI SfcI SmaI SmlI SpeI SphI StuI TaqII
TatI Tth111I XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BcgI BciVI BglI BglII BlpI
BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
Bsu36I BtgI ClaI CspCI DraI DraIII EagI EciI
EcoRV FspI FspAI HincII HpaI KasI KpnI MfeI
MluI NarI NcoI NdeI NheI NotI NruI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SrfI
SspI StyI SwaI TstI XcmI XhoI ZraI