Restriction map for
pFN21AE2229
(Linear) (Six-base) MAPSORT of: KIEE2229.seq Check: 1599 from: 1 to: 735
KIEE2229.orf
With 182 enzymes: SgfI *
July 15, 2010 18:56 ..
AccI GT'mk_AC
Cuts at: 0 694 735
Size: 694 41
AflIII A'CryG_T
Cuts at: 0 462 735
Size: 462 273
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 183 735
Size: 183 552
AlwNI CAG_nnn'CTG
Cuts at: 0 350 735
Size: 350 385
ApaI G_GGCC'C
Cuts at: 0 324 735
Size: 324 411
AvaI C'yCGr_G
Cuts at: 0 28 317 735
Size: 28 289 418
Fragments arranged by size:
418 289 28
BanI G'GyrC_C
Cuts at: 0 415 435 735
Size: 415 20 300
Fragments arranged by size:
415 300 20
BanII G_rGCy'C
Cuts at: 0 108 261 298 324 735
Size: 108 153 37 26 411
Fragments arranged by size:
411 153 108 37 26
BbvCI CC'TCA_GC
Cuts at: 0 343 735
Size: 343 392
BciVI GTATCCnnnnn_n'
Cuts at: 0 662 735
Size: 662 73
BglI GCCn_nnn'nGGC
Cuts at: 0 480 735
Size: 480 255
Bme1580I G_kGCm'C
Cuts at: 0 207 324 420 440 735
Size: 207 117 96 20 295
Fragments arranged by size:
295 207 117 96 20
BmgBI CAC'GTC
Cuts at: 0 219 735
Size: 219 516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 256 559 580 735
Size: 245 11 303 21 155
Fragments arranged by size:
303 245 155 21 11
Bpu10I CC'TnA_GC
Cuts at: 0 343 455 735
Size: 343 112 280
Fragments arranged by size:
343 280 112
BsaWI w'CCGG_w
Cuts at: 0 108 735
Size: 108 627
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 735
Size: 25 710
BseYI C'CCAG_C
Cuts at: 0 101 376 568 619 735
Size: 101 275 192 51 116
Fragments arranged by size:
275 192 116 101 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 735
Size: 336 399
Bsp1286I G_dGCh'C
Cuts at: 0 108 207 261 298 324 420 440 735
Size: 108 99 54 37 26 96 20 295
Fragments arranged by size:
295 108 99 96 54 37 26 20
BspEI T'CCGG_A
Cuts at: 0 108 735
Size: 108 627
BsrDI GCAATG_nn'
Cuts at: 0 450 735
Size: 450 285
BstXI CCAn_nnnn'nTGG
Cuts at: 0 280 735
Size: 280 455
BtsI GCAGTG_nn'
Cuts at: 0 167 735
Size: 167 568
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 459 494 735
Size: 459 35 241
Fragments arranged by size:
459 241 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 510 735
Size: 510 225
EaeI y'GGCC_r
Cuts at: 0 128 429 735
Size: 128 301 306
Fragments arranged by size:
306 301 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 256 559 580 735
Size: 245 11 303 21 155
Fragments arranged by size:
303 245 155 21 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 28 361 735
Size: 28 333 374
Fragments arranged by size:
374 333 28
EcoO109I rG'GnC_Cy
Cuts at: 0 321 389 735
Size: 321 68 346
Fragments arranged by size:
346 321 68
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 692 735
Size: 692 43
HaeII r_GCGC'y
Cuts at: 0 342 735
Size: 342 393
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 735
Size: 539 196
MscI TGG'CCA
Cuts at: 0 130 735
Size: 130 605
MslI CAynn'nnrTG
Cuts at: 0 92 735
Size: 92 643
MspA1I CmG'CkG
Cuts at: 0 101 276 619 735
Size: 101 175 343 116
Fragments arranged by size:
343 175 116 101
NspI r_CATG'y
Cuts at: 0 466 735
Size: 466 269
PasI CC'CwG_GG
Cuts at: 0 298 357 735
Size: 298 59 378
Fragments arranged by size:
378 298 59
PciI A'CATG_T
Cuts at: 0 462 735
Size: 462 273
PflMI CCAn_nnn'nTGG
Cuts at: 0 126 735
Size: 126 609
PfoI T'CCnGG_A
Cuts at: 0 212 735
Size: 212 523
PpuMI rG'GwC_Cy
Cuts at: 0 389 735
Size: 389 346
PspOMI G'GGCC_C
Cuts at: 0 320 735
Size: 320 415
PvuII CAG'CTG
Cuts at: 0 276 735
Size: 276 459
SmaI CCC'GGG
Cuts at: 0 319 735
Size: 319 416
SrfI GCCC'GGGC
Cuts at: 0 319 735
Size: 319 416
StuI AGG'CCT
Cuts at: 0 236 584 735
Size: 236 348 151
Fragments arranged by size:
348 236 151
StyI C'CwwG_G
Cuts at: 0 667 735
Size: 667 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 595 627 735
Size: 595 32 108
Fragments arranged by size:
595 108 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 13 735
Size: 13 722
XmaI C'CCGG_G
Cuts at: 0 317 735
Size: 317 418
Enzymes that do cut:
AccI AflIII AlfI AlwNI ApaI AvaI BanI BanII
BbvCI BciVI BglI Bme1580I BmgBI BpmI Bpu10I BsaWI
BseRI BseYI BsgI Bsp1286I BspEI BsrDI BstXI BtsI
CspCI DrdI EaeI Eco57MI EcoNI EcoO109I FalI HaeII
MmeI MscI MslI MspA1I NspI PasI PciI PflMI
PfoI PpuMI PspOMI PvuII SmaI SrfI StuI StyI
TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BcgI BclI BfrBI
BglII BlpI BmrI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
DraI DraIII EagI EarI EciI EcoICRI EcoRI EcoRV
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SspI SwaI TaqII TatI Tth111I XbaI XhoI XmnI
ZraI