Restriction map for
pFN21AE2223
(Linear) (Six-base) MAPSORT of: KIEE2223.seq Check: 8822 from: 1 to: 1014
KIEE2223.orf
With 182 enzymes: SgfI *
July 15, 2010 18:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 689 1014
Size: 689 325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 904 1014
Size: 60 844 110
Fragments arranged by size:
844 110 60
AlwNI CAG_nnn'CTG
Cuts at: 0 140 237 914 1014
Size: 140 97 677 100
Fragments arranged by size:
677 140 100 97
ApaI G_GGCC'C
Cuts at: 0 30 694 1014
Size: 30 664 320
Fragments arranged by size:
664 320 30
AvaI C'yCGr_G
Cuts at: 0 62 179 1014
Size: 62 117 835
Fragments arranged by size:
835 117 62
BamHI G'GATC_C
Cuts at: 0 466 1014
Size: 466 548
BanI G'GyrC_C
Cuts at: 0 378 840 893 1014
Size: 378 462 53 121
Fragments arranged by size:
462 378 121 53
BanII G_rGCy'C
Cuts at: 0 30 365 694 1014
Size: 30 335 329 320
Fragments arranged by size:
335 329 320 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 720 782 1014
Size: 720 62 232
Fragments arranged by size:
720 232 62
BbvCI CC'TCA_GC
Cuts at: 0 523 1014
Size: 523 491
BciVI GTATCCnnnnn_n'
Cuts at: 0 178 298 1014
Size: 178 120 716
Fragments arranged by size:
716 178 120
BlpI GC'TnA_GC
Cuts at: 0 364 532 1014
Size: 364 168 482
Fragments arranged by size:
482 364 168
Bme1580I G_kGCm'C
Cuts at: 0 30 383 694 1014
Size: 30 353 311 320
Fragments arranged by size:
353 320 311 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 155 379 1014
Size: 155 224 635
Fragments arranged by size:
635 224 155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 1014
Size: 511 503
Bpu10I CC'TnA_GC
Cuts at: 0 523 1014
Size: 523 491
BsaBI GATnn'nnATC
Cuts at: 0 637 1014
Size: 637 377
BsaWI w'CCGG_w
Cuts at: 0 998 1014
Size: 998 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 42 72 833 863 1014
Size: 42 30 761 30 151
Fragments arranged by size:
761 151 42 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 63 817 1014
Size: 63 754 197
Fragments arranged by size:
754 197 63
BseYI C'CCAG_C
Cuts at: 0 68 106 257 1014
Size: 68 38 151 757
Fragments arranged by size:
757 151 68 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 1014
Size: 349 665
BsmI GAATG_Cn'
Cuts at: 0 23 609 1014
Size: 23 586 405
Fragments arranged by size:
586 405 23
Bsp1286I G_dGCh'C
Cuts at: 0 30 365 383 694 1014
Size: 30 335 18 311 320
Fragments arranged by size:
335 320 311 30 18
BspEI T'CCGG_A
Cuts at: 0 998 1014
Size: 998 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 689 1014
Size: 689 325
BsrDI GCAATG_nn'
Cuts at: 0 557 1014
Size: 557 457
BsrFI r'CCGG_y
Cuts at: 0 243 324 926 1014
Size: 243 81 602 88
Fragments arranged by size:
602 243 88 81
BstAPI GCAn_nnn'nTGC
Cuts at: 0 321 1014
Size: 321 693
BstXI CCAn_nnnn'nTGG
Cuts at: 0 129 145 1014
Size: 129 16 869
Fragments arranged by size:
869 129 16
BstYI r'GATC_y
Cuts at: 0 466 761 1014
Size: 466 295 253
Fragments arranged by size:
466 295 253
Bsu36I CC'TnA_GG
Cuts at: 0 173 238 370 1014
Size: 173 65 132 644
Fragments arranged by size:
644 173 132 65
BtgI C'CryG_G
Cuts at: 0 397 695 782 1014
Size: 397 298 87 232
Fragments arranged by size:
397 298 232 87
DrdI GACnn_nn'nnGTC
Cuts at: 0 706 1014
Size: 706 308
EaeI y'GGCC_r
Cuts at: 0 400 1014
Size: 400 614
EarI CTCTTCn'nnn_
Cuts at: 0 154 431 1014
Size: 154 277 583
Fragments arranged by size:
583 277 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 511 904 1014
Size: 60 451 393 110
Fragments arranged by size:
451 393 110 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 663 1014
Size: 663 351
EcoO109I rG'GnC_Cy
Cuts at: 0 27 452 690 691 980 1014
Size: 27 425 238 1 289 34
Fragments arranged by size:
425 289 238 34 27 1
FspI TGC'GCA
Cuts at: 0 961 1014
Size: 961 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 291 323 643 675 1014
Size: 291 32 320 32 339
Fragments arranged by size:
339 320 291 32 32
MslI CAynn'nnrTG
Cuts at: 0 562 602 1014
Size: 562 40 412
Fragments arranged by size:
562 412 40
MspA1I CmG'CkG
Cuts at: 0 399 750 784 914 1014
Size: 399 351 34 130 100
Fragments arranged by size:
399 351 130 100 34
NaeI GCC'GGC
Cuts at: 0 245 928 1014
Size: 245 683 86
Fragments arranged by size:
683 245 86
NcoI C'CATG_G
Cuts at: 0 695 1014
Size: 695 319
NgoMIV G'CCGG_C
Cuts at: 0 243 926 1014
Size: 243 683 88
Fragments arranged by size:
683 243 88
PasI CC'CwG_GG
Cuts at: 0 43 686 1014
Size: 43 643 328
Fragments arranged by size:
643 328 43
PflMI CCAn_nnn'nTGG
Cuts at: 0 462 741 831 1014
Size: 462 279 90 183
Fragments arranged by size:
462 279 183 90
PfoI T'CCnGG_A
Cuts at: 0 290 1014
Size: 290 724
PpuMI rG'GwC_Cy
Cuts at: 0 452 1014
Size: 452 562
PshAI GACnn'nnGTC
Cuts at: 0 148 1014
Size: 148 866
PspOMI G'GGCC_C
Cuts at: 0 26 690 1014
Size: 26 664 324
Fragments arranged by size:
664 324 26
PvuII CAG'CTG
Cuts at: 0 750 914 1014
Size: 750 164 100
Fragments arranged by size:
750 164 100
SacII CC_GC'GG
Cuts at: 0 400 785 1014
Size: 400 385 229
Fragments arranged by size:
400 385 229
SfcI C'TryA_G
Cuts at: 0 280 1014
Size: 280 734
SmaI CCC'GGG
Cuts at: 0 64 181 1014
Size: 64 117 833
Fragments arranged by size:
833 117 64
StyI C'CwwG_G
Cuts at: 0 213 339 695 836 889 1014
Size: 213 126 356 141 53 125
Fragments arranged by size:
356 213 141 126 125 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 417 1014
Size: 417 597
TatI w'GTAC_w
Cuts at: 0 194 332 1014
Size: 194 138 682
Fragments arranged by size:
682 194 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 833 865 1014
Size: 833 32 149
Fragments arranged by size:
833 149 32
XmaI C'CCGG_G
Cuts at: 0 62 179 1014
Size: 62 117 835
Fragments arranged by size:
835 117 62
Enzymes that do cut:
AarI AcuI AlwNI ApaI AvaI BamHI BanI BanII
BbsI BbvCI BciVI BlpI Bme1580I BmrI BpmI Bpu10I
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I
BspEI BspMI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I
BtgI DrdI EaeI EarI Eco57MI EcoNI EcoO109I FspI
Hin4I MslI MspA1I NaeI NcoI NgoMIV PasI PflMI
PfoI PpuMI PshAI PspOMI PvuII SacII SfcI SmaI
StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BcgI BclI BfrBI BglI
BglII BmgBI BmtI BplI BpuEI BsaI BsaAI BsaHI
BsiEI BsiHKAI BsiWI BsmBI BspHI BsrBI BsrGI BssHII
BssSI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NarI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PsiI PspXI PsrI PstI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XhoI XmnI ZraI