Restriction map for
pFN21AE2222
(Linear) (Six-base) MAPSORT of: KIEE2222.seq Check: 9007 from: 1 to: 738
KIEE2222.orf
With 182 enzymes: SgfI *
July 3, 2012 17:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 738
Size: 680 58
AgeI A'CCGG_T
Cuts at: 0 155 738
Size: 155 583
AlwNI CAG_nnn'CTG
Cuts at: 0 88 207 247 738
Size: 88 119 40 491
Fragments arranged by size:
491 119 88 40
ApoI r'AATT_y
Cuts at: 0 631 738
Size: 631 107
AvaI C'yCGr_G
Cuts at: 0 396 418 738
Size: 396 22 320
Fragments arranged by size:
396 320 22
BanI G'GyrC_C
Cuts at: 0 197 465 738
Size: 197 268 273
Fragments arranged by size:
273 268 197
BbeI G_GCGC'C
Cuts at: 0 469 738
Size: 469 269
BglII A'GATC_T
Cuts at: 0 333 738
Size: 333 405
BlpI GC'TnA_GC
Cuts at: 0 89 738
Size: 89 649
Bme1580I G_kGCm'C
Cuts at: 0 379 727 738
Size: 379 348 11
Fragments arranged by size:
379 348 11
BmgBI CAC'GTC
Cuts at: 0 387 738
Size: 387 351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 276 738
Size: 202 74 462
Fragments arranged by size:
462 202 74
BsaHI Gr'CG_yC
Cuts at: 0 466 738
Size: 466 272
BsaWI w'CCGG_w
Cuts at: 0 155 738
Size: 155 583
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 630 738
Size: 630 108
BseYI C'CCAG_C
Cuts at: 0 301 738
Size: 301 437
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 738
Size: 292 446
BsiHKAI G_wGCw'C
Cuts at: 0 95 738
Size: 95 643
Bsp1286I G_dGCh'C
Cuts at: 0 95 379 727 738
Size: 95 284 348 11
Fragments arranged by size:
348 284 95 11
BspHI T'CATG_A
Cuts at: 0 498 738
Size: 498 240
BsrFI r'CCGG_y
Cuts at: 0 155 738
Size: 155 583
BstXI CCAn_nnnn'nTGG
Cuts at: 0 58 738
Size: 58 680
BstYI r'GATC_y
Cuts at: 0 297 333 738
Size: 297 36 405
Fragments arranged by size:
405 297 36
BtgI C'CryG_G
Cuts at: 0 711 738
Size: 711 27
EaeI y'GGCC_r
Cuts at: 0 698 738
Size: 698 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 276 680 738
Size: 202 74 404 58
Fragments arranged by size:
404 202 74 58
EcoO109I rG'GnC_Cy
Cuts at: 0 120 251 489 738
Size: 120 131 238 249
Fragments arranged by size:
249 238 131 120
EcoRI G'AATT_C
Cuts at: 0 631 738
Size: 631 107
HaeII r_GCGC'y
Cuts at: 0 89 170 469 738
Size: 89 81 299 269
Fragments arranged by size:
299 269 89 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 96 128 289 321 738
Size: 96 32 161 32 417
Fragments arranged by size:
417 161 96 32 32
HincII GTy'rAC
Cuts at: 0 282 738
Size: 282 456
KasI G'GCGC_C
Cuts at: 0 465 738
Size: 465 273
MscI TGG'CCA
Cuts at: 0 700 738
Size: 700 38
NarI GG'CG_CC
Cuts at: 0 466 738
Size: 466 272
PfoI T'CCnGG_A
Cuts at: 0 179 485 738
Size: 179 306 253
Fragments arranged by size:
306 253 179
PpuMI rG'GwC_Cy
Cuts at: 0 120 489 738
Size: 120 369 249
Fragments arranged by size:
369 249 120
PspXI vC'TCGA_Gb
Cuts at: 0 418 738
Size: 418 320
SanDI GG'GwC_CC
Cuts at: 0 120 738
Size: 120 618
SfoI GGC'GCC
Cuts at: 0 467 738
Size: 467 271
SmlI C'TyrA_G
Cuts at: 0 418 738
Size: 418 320
TatI w'GTAC_w
Cuts at: 0 689 738
Size: 689 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 398 430 738
Size: 398 32 308
Fragments arranged by size:
398 308 32
XhoI C'TCGA_G
Cuts at: 0 418 738
Size: 418 320
Enzymes that do cut:
AcuI AgeI AlwNI ApoI AvaI BanI BbeI BglII
BlpI Bme1580I BmgBI BpmI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspHI BsrFI BstXI BstYI BtgI
EaeI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII KasI
MscI NarI PfoI PpuMI PspXI SanDI SfoI SmlI
TatI TstI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII Tth111I XbaI XcmI XmaI XmnI
ZraI