Restriction map for
pFN21AE2216
(Linear) (Six-base) MAPSORT of: KIEE2216.seq Check: 7475 from: 1 to: 657
KIEE2216.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AflIII A'CryG_T
Cuts at: 0 287 410 657
Size: 287 123 247
Fragments arranged by size:
287 247 123
AhdI GACnn_n'nnGTC
Cuts at: 0 76 657
Size: 76 581
AlwNI CAG_nnn'CTG
Cuts at: 0 174 276 657
Size: 174 102 381
Fragments arranged by size:
381 174 102
ApoI r'AATT_y
Cuts at: 0 584 657
Size: 584 73
AvaI C'yCGr_G
Cuts at: 0 428 657
Size: 428 229
BanII G_rGCy'C
Cuts at: 0 328 476 533 657
Size: 328 148 57 124
Fragments arranged by size:
328 148 124 57
BbvCI CC'TCA_GC
Cuts at: 0 87 657
Size: 87 570
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 409 443 657
Size: 409 34 214
Fragments arranged by size:
409 214 34
BclI T'GATC_A
Cuts at: 0 123 657
Size: 123 534
BlpI GC'TnA_GC
Cuts at: 0 620 657
Size: 620 37
BmgBI CAC'GTC
Cuts at: 0 411 657
Size: 411 246
BmrI ACTGGGnnnn_n'
Cuts at: 0 20 657
Size: 20 637
BmtI G_CTAG'C
Cuts at: 0 181 657
Size: 181 476
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 657
Size: 27 630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 342 657
Size: 298 44 315
Fragments arranged by size:
315 298 44
Bpu10I CC'TnA_GC
Cuts at: 0 87 657
Size: 87 570
BsaI GGTCTCn'nnnn_
Cuts at: 0 187 425 657
Size: 187 238 232
Fragments arranged by size:
238 232 187
BsaAI yAC'GTr
Cuts at: 0 290 657
Size: 290 367
BsaWI w'CCGG_w
Cuts at: 0 140 657
Size: 140 517
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 75 105 657
Size: 75 30 552
Fragments arranged by size:
552 75 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 247 657
Size: 247 410
BseYI C'CCAG_C
Cuts at: 0 101 579 657
Size: 101 478 78
Fragments arranged by size:
478 101 78
BsiHKAI G_wGCw'C
Cuts at: 0 533 657
Size: 533 124
Bsp1286I G_dGCh'C
Cuts at: 0 328 476 533 657
Size: 328 148 57 124
Fragments arranged by size:
328 148 124 57
BspEI T'CCGG_A
Cuts at: 0 140 657
Size: 140 517
BsrDI GCAATG_nn'
Cuts at: 0 389 657
Size: 389 268
BsrGI T'GTAC_A
Cuts at: 0 413 657
Size: 413 244
BstXI CCAn_nnnn'nTGG
Cuts at: 0 189 657
Size: 189 468
BtsI GCAGTG_nn'
Cuts at: 0 254 550 614 631 657
Size: 254 296 64 17 26
Fragments arranged by size:
296 254 64 26 17
EcoICRI GAG'CTC
Cuts at: 0 531 657
Size: 531 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 342 657
Size: 298 44 315
Fragments arranged by size:
315 298 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 62 75 94 107 657
Size: 62 13 19 13 550
Fragments arranged by size:
550 62 19 13 13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 657
Size: 520 137
MspA1I CmG'CkG
Cuts at: 0 157 273 657
Size: 157 116 384
Fragments arranged by size:
384 157 116
NdeI CA'TA_TG
Cuts at: 0 351 572 657
Size: 351 221 85
Fragments arranged by size:
351 221 85
NheI G'CTAG_C
Cuts at: 0 177 657
Size: 177 480
PflMI CCAn_nnn'nTGG
Cuts at: 0 276 657
Size: 276 381
PmlI CAC'GTG
Cuts at: 0 290 657
Size: 290 367
PstI C_TGCA'G
Cuts at: 0 249 657
Size: 249 408
PvuII CAG'CTG
Cuts at: 0 157 273 657
Size: 157 116 384
Fragments arranged by size:
384 157 116
SacI G_AGCT'C
Cuts at: 0 533 657
Size: 533 124
ScaI AGT'ACT
Cuts at: 0 521 657
Size: 521 136
SfcI C'TryA_G
Cuts at: 0 245 657
Size: 245 412
SmlI C'TyrA_G
Cuts at: 0 428 657
Size: 428 229
StuI AGG'CCT
Cuts at: 0 149 563 657
Size: 149 414 94
Fragments arranged by size:
414 149 94
TatI w'GTAC_w
Cuts at: 0 413 519 657
Size: 413 106 138
Fragments arranged by size:
413 138 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 233 265 274 306 657
Size: 233 32 9 32 351
Fragments arranged by size:
351 233 32 32 9
XhoI C'TCGA_G
Cuts at: 0 428 657
Size: 428 229
Enzymes that do cut:
AflIII AhdI AlwNI ApoI AvaI BanII BbvCI BcgI
BclI BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I
BsaI BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I
BspEI BsrDI BsrGI BstXI BtsI EcoICRI Eco57MI Hin4I
MmeI MspA1I NdeI NheI PflMI PmlI PstI PvuII
SacI ScaI SfcI SmlI StuI TatI TstI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BciVI
BfrBI BglI BglII Bme1580I BpuEI BsaBI BsaHI BsgI
BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MscI MslI NaeI NarI NcoI
NgoMIV NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StyI SwaI TaqII Tth111I XbaI
XcmI XmaI XmnI ZraI