Restriction map for
pFN21AE2210
(Linear) (Six-base) MAPSORT of: KIEE2210.seq Check: 6887 from: 1 to: 2637
KIEE2210.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 579 2637
Size: 579 2058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 1625 2227 2467 2637
Size: 853 772 602 240 170
Fragments arranged by size:
853 772 602 240 170
AflIII A'CryG_T
Cuts at: 0 1445 2637
Size: 1445 1192
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1678 2637
Size: 1678 959
ApaI G_GGCC'C
Cuts at: 0 213 2637
Size: 213 2424
ApoI r'AATT_y
Cuts at: 0 265 1483 1558 1964 1984 1993 2512 2584
Size: 265 1218 75 406 20 9 519 72
Cuts at: 2584 2637
Size: 53
Fragments arranged by size:
1218 519 406 265 75 72 53 20
9
BanII G_rGCy'C
Cuts at: 0 213 1526 2637
Size: 213 1313 1111
Fragments arranged by size:
1313 1111 213
BbsI GAAGACnn'nnnn_
Cuts at: 0 1324 2125 2637
Size: 1324 801 512
Fragments arranged by size:
1324 801 512
BclI T'GATC_A
Cuts at: 0 1080 2637
Size: 1080 1557
BfrBI ATG'CAT
Cuts at: 0 19 328 341 1904 2637
Size: 19 309 13 1563 733
Fragments arranged by size:
1563 733 309 19 13
BglII A'GATC_T
Cuts at: 0 219 752 879 1411 1493 1940 2637
Size: 219 533 127 532 82 447 697
Fragments arranged by size:
697 533 532 447 219 127 82
BlpI GC'TnA_GC
Cuts at: 0 61 2030 2637
Size: 61 1969 607
Fragments arranged by size:
1969 607 61
Bme1580I G_kGCm'C
Cuts at: 0 213 2637
Size: 213 2424
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1109 1171 2637
Size: 1109 62 1466
Fragments arranged by size:
1466 1109 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1540 2637
Size: 1540 1097
BsaI GGTCTCn'nnnn_
Cuts at: 0 2036 2637
Size: 2036 601
BsaWI w'CCGG_w
Cuts at: 0 368 2637
Size: 368 2269
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2069 2099 2637
Size: 2069 30 538
Fragments arranged by size:
2069 538 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2090 2353 2637
Size: 2090 263 284
Fragments arranged by size:
2090 284 263
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 894 1805 2637
Size: 163 731 911 832
Fragments arranged by size:
911 832 731 163
BsmI GAATG_Cn'
Cuts at: 0 330 555 2637
Size: 330 225 2082
Fragments arranged by size:
2082 330 225
Bsp1286I G_dGCh'C
Cuts at: 0 213 1526 2637
Size: 213 1313 1111
Fragments arranged by size:
1313 1111 213
BspEI T'CCGG_A
Cuts at: 0 368 2637
Size: 368 2269
BspHI T'CATG_A
Cuts at: 0 1077 2637
Size: 1077 1560
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 135 579 2637
Size: 135 444 2058
Fragments arranged by size:
2058 444 135
BsrDI GCAATG_nn'
Cuts at: 0 2621 2637
Size: 2621 16
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2502 2637
Size: 2502 135
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1173 2133 2637
Size: 1173 960 504
Fragments arranged by size:
1173 960 504
BstYI r'GATC_y
Cuts at: 0 219 752 879 1360 1411 1493 1940 2566
Size: 219 533 127 481 51 82 447 626
Cuts at: 2566 2637
Size: 71
Fragments arranged by size:
626 533 481 447 219 127 82 71
51
BtsI GCAGTG_nn'
Cuts at: 0 311 943 1660 2637
Size: 311 632 717 977
Fragments arranged by size:
977 717 632 311
DrdI GACnn_nn'nnGTC
Cuts at: 0 1043 2637
Size: 1043 1594
EaeI y'GGCC_r
Cuts at: 0 845 1040 2637
Size: 845 195 1597
Fragments arranged by size:
1597 845 195
EarI CTCTTCn'nnn_
Cuts at: 0 959 2465 2637
Size: 959 1506 172
Fragments arranged by size:
1506 959 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1681 2637
Size: 1681 956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 1540 1625 2227 2467 2637
Size: 853 687 85 602 240 170
Fragments arranged by size:
853 687 602 240 170 85
EcoO109I rG'GnC_Cy
Cuts at: 0 210 864 1614 2637
Size: 210 654 750 1023
Fragments arranged by size:
1023 750 654 210
EcoRI G'AATT_C
Cuts at: 0 1964 2584 2637
Size: 1964 620 53
Fragments arranged by size:
1964 620 53
EcoRV GAT'ATC
Cuts at: 0 364 510 2637
Size: 364 146 2127
Fragments arranged by size:
2127 364 146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 898 2637
Size: 898 1739
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 739 771 1225 1257 2171 2203 2637
Size: 739 32 454 32 914 32 434
Fragments arranged by size:
914 739 454 434 32 32 32
HincII GTy'rAC
Cuts at: 0 642 2637
Size: 642 1995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1412 1941 2637
Size: 1412 529 696
Fragments arranged by size:
1412 696 529
MscI TGG'CCA
Cuts at: 0 847 1042 2637
Size: 847 195 1595
Fragments arranged by size:
1595 847 195
MslI CAynn'nnrTG
Cuts at: 0 649 1649 2462 2637
Size: 649 1000 813 175
Fragments arranged by size:
1000 813 649 175
NsiI A_TGCA'T
Cuts at: 0 21 330 343 1906 2637
Size: 21 309 13 1563 731
Fragments arranged by size:
1563 731 309 21 13
NspI r_CATG'y
Cuts at: 0 19 1449 2637
Size: 19 1430 1188
Fragments arranged by size:
1430 1188 19
PasI CC'CwG_GG
Cuts at: 0 2126 2637
Size: 2126 511
PciI A'CATG_T
Cuts at: 0 1445 2637
Size: 1445 1192
PfoI T'CCnGG_A
Cuts at: 0 2562 2637
Size: 2562 75
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 805 837 2637
Size: 805 32 1800
Fragments arranged by size:
1800 805 32
PpuMI rG'GwC_Cy
Cuts at: 0 1614 2637
Size: 1614 1023
PspOMI G'GGCC_C
Cuts at: 0 209 2637
Size: 209 2428
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1106 1138 2637
Size: 1106 32 1499
Fragments arranged by size:
1499 1106 32
PstI C_TGCA'G
Cuts at: 0 590 938 2637
Size: 590 348 1699
Fragments arranged by size:
1699 590 348
SapI GCTCTTCn'nnn_
Cuts at: 0 959 2637
Size: 959 1678
ScaI AGT'ACT
Cuts at: 0 1854 2637
Size: 1854 783
SfcI C'TryA_G
Cuts at: 0 586 934 1570 2637
Size: 586 348 636 1067
Fragments arranged by size:
1067 636 586 348
SphI G_CATG'C
Cuts at: 0 19 2637
Size: 19 2618
SspI AAT'ATT
Cuts at: 0 1712 2637
Size: 1712 925
StuI AGG'CCT
Cuts at: 0 907 2637
Size: 907 1730
TatI w'GTAC_w
Cuts at: 0 1189 1852 2637
Size: 1189 663 785
Fragments arranged by size:
1189 785 663
XbaI T'CTAG_A
Cuts at: 0 2196 2427 2637
Size: 2196 231 210
Fragments arranged by size:
2196 231 210
XmnI GAAnn'nnTTC
Cuts at: 0 419 2150 2637
Size: 419 1731 487
Fragments arranged by size:
1731 487 419
Enzymes that do cut:
AarI AcuI AflIII AlfI ApaI ApoI BanII BbsI
BclI BfrBI BglII BlpI Bme1580I BplI BpmI BsaI
BsaWI BsaXI BseRI BsgI BsmI Bsp1286I BspEI BspHI
BspMI BsrDI BstAPI BstXI BstYI BtsI DrdI EaeI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I
HincII MmeI MscI MslI NsiI NspI PasI PciI
PfoI PpiI PpuMI PspOMI PsrI PstI SapI ScaI
SfcI SphI SspI StuI TatI XbaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BglI BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaBI
BsaHI BseYI BsiEI BsiHKAI BsiWI BsmBI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII EagI EcoICRI EcoNI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PflMI PmeI PmlI PshAI
PsiI PspXI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI StyI SwaI TaqII TstI
Tth111I XcmI XhoI XmaI ZraI