Restriction map for
pFN21AE2208
(Linear) (Six-base) MAPSORT of: KIEE2208.seq Check: 615 from: 1 to: 768
KIEE2208.orf
With 182 enzymes: SgfI *
July 3, 2012 17:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 502 768
Size: 322 180 266
Fragments arranged by size:
322 266 180
AleI CACnn'nnGTG
Cuts at: 0 118 768
Size: 118 650
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 349 381 768
Size: 349 32 387
Fragments arranged by size:
387 349 32
AlwNI CAG_nnn'CTG
Cuts at: 0 376 711 726 768
Size: 376 335 15 42
Fragments arranged by size:
376 335 42 15
ApaLI G'TGCA_C
Cuts at: 0 121 768
Size: 121 647
ApoI r'AATT_y
Cuts at: 0 577 768
Size: 577 191
AvaI C'yCGr_G
Cuts at: 0 532 541 768
Size: 532 9 227
Fragments arranged by size:
532 227 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 31 768
Size: 31 737
BamHI G'GATC_C
Cuts at: 0 254 768
Size: 254 514
BanII G_rGCy'C
Cuts at: 0 422 768
Size: 422 346
BciVI GTATCCnnnnn_n'
Cuts at: 0 26 768
Size: 26 742
BglI GCCn_nnn'nGGC
Cuts at: 0 74 768
Size: 74 694
BglII A'GATC_T
Cuts at: 0 683 768
Size: 683 85
Bme1580I G_kGCm'C
Cuts at: 0 125 197 768
Size: 125 72 571
Fragments arranged by size:
571 125 72
BmgBI CAC'GTC
Cuts at: 0 192 768
Size: 192 576
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 643 768
Size: 115 528 125
Fragments arranged by size:
528 125 115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 768
Size: 421 347
BsaHI Gr'CG_yC
Cuts at: 0 109 768
Size: 109 659
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 73 99 103 129 274 304 442 445
Size: 73 26 4 26 145 30 138 3
Cuts at: 445 472 475 589 619 768
Size: 27 3 114 30 149
Fragments arranged by size:
149 145 138 114 73 30 30 27
26 26 4 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 115 607 610 640 670 768
Size: 112 3 492 3 30 30 98
Fragments arranged by size:
492 112 98 30 30 3 3
BseYI C'CCAG_C
Cuts at: 0 224 521 768
Size: 224 297 247
Fragments arranged by size:
297 247 224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 766 768
Size: 511 255 2
Fragments arranged by size:
511 255 2
BsiHKAI G_wGCw'C
Cuts at: 0 125 463 768
Size: 125 338 305
Fragments arranged by size:
338 305 125
Bsp1286I G_dGCh'C
Cuts at: 0 125 197 422 463 768
Size: 125 72 225 41 305
Fragments arranged by size:
305 225 125 72 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 92 768
Size: 92 676
BsrBI CCG'CTC
Cuts at: 0 105 546 768
Size: 105 441 222
Fragments arranged by size:
441 222 105
BsrFI r'CCGG_y
Cuts at: 0 735 768
Size: 735 33
BstYI r'GATC_y
Cuts at: 0 254 350 683 768
Size: 254 96 333 85
Fragments arranged by size:
333 254 96 85
ClaI AT'CG_AT
Cuts at: 0 404 768
Size: 404 364
EaeI y'GGCC_r
Cuts at: 0 75 762 768
Size: 75 687 6
Fragments arranged by size:
687 75 6
EarI CTCTTCn'nnn_
Cuts at: 0 745 768
Size: 745 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 709 768
Size: 709 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 322 502 643 768
Size: 115 207 180 141 125
Fragments arranged by size:
207 180 141 125 115
EcoNI CCTnn'n_nnAGG
Cuts at: 0 97 768
Size: 97 671
EcoO109I rG'GnC_Cy
Cuts at: 0 453 516 537 663 768
Size: 453 63 21 126 105
Fragments arranged by size:
453 126 105 63 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 99 131 274 306 445 477 768
Size: 99 32 143 32 139 32 291
Fragments arranged by size:
291 143 139 99 32 32 32
MscI TGG'CCA
Cuts at: 0 764 768
Size: 764 4
MslI CAynn'nnrTG
Cuts at: 0 118 768
Size: 118 650
MspA1I CmG'CkG
Cuts at: 0 45 174 228 324 525 711 768
Size: 45 129 54 96 201 186 57
Fragments arranged by size:
201 186 129 96 57 54 45
NaeI GCC'GGC
Cuts at: 0 737 768
Size: 737 31
NgoMIV G'CCGG_C
Cuts at: 0 735 768
Size: 735 33
PfoI T'CCnGG_A
Cuts at: 0 353 768
Size: 353 415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 322 354 768
Size: 322 32 414
Fragments arranged by size:
414 322 32
PpuMI rG'GwC_Cy
Cuts at: 0 453 516 537 663 768
Size: 453 63 21 126 105
Fragments arranged by size:
453 126 105 63 21
PstI C_TGCA'G
Cuts at: 0 44 716 768
Size: 44 672 52
Fragments arranged by size:
672 52 44
PvuII CAG'CTG
Cuts at: 0 45 324 525 711 768
Size: 45 279 201 186 57
Fragments arranged by size:
279 201 186 57 45
SfcI C'TryA_G
Cuts at: 0 40 126 342 712 768
Size: 40 86 216 370 56
Fragments arranged by size:
370 216 86 56 40
SmlI C'TyrA_G
Cuts at: 0 436 768
Size: 436 332
StuI AGG'CCT
Cuts at: 0 132 768
Size: 132 636
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 597 768
Size: 597 171
TatI w'GTAC_w
Cuts at: 0 569 768
Size: 569 199
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 442 474 589 621 768
Size: 442 32 115 32 147
Fragments arranged by size:
442 147 115 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 390 471 768
Size: 390 81 297
Fragments arranged by size:
390 297 81
Enzymes that do cut:
AcuI AleI AloI AlwNI ApaLI ApoI AvaI BaeI
BamHI BanII BciVI BglI BglII Bme1580I BmgBI BpmI
BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BstYI ClaI EaeI EarI EciI
Eco57MI EcoNI EcoO109I Hin4I MscI MslI MspA1I NaeI
NgoMIV PfoI PpiI PpuMI PstI PvuII SfcI SmlI
StuI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI ApaI AscI AseI AsiSI AvrII
BanI BbeI BbsI BbvCI BcgI BclI BfrBI BlpI
BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EagI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI
SwaI Tth111I XbaI XhoI XmaI XmnI ZraI