Restriction map for
pFN21AE2207
(Linear) (Six-base) MAPSORT of: KIEE2207.seq Check: 577 from: 1 to: 957
KIEE2207.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 957
Size: 859 98
AlwNI CAG_nnn'CTG
Cuts at: 0 402 436 957
Size: 402 34 521
Fragments arranged by size:
521 402 34
ApaI G_GGCC'C
Cuts at: 0 325 957
Size: 325 632
AseI AT'TA_AT
Cuts at: 0 248 957
Size: 248 709
AvaI C'yCGr_G
Cuts at: 0 935 957
Size: 935 22
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 759 792 957
Size: 759 33 165
Fragments arranged by size:
759 165 33
BamHI G'GATC_C
Cuts at: 0 3 866 957
Size: 3 863 91
Fragments arranged by size:
863 91 3
BanII G_rGCy'C
Cuts at: 0 325 957
Size: 325 632
Bme1580I G_kGCm'C
Cuts at: 0 325 957
Size: 325 632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 940 957
Size: 940 17
BsaWI w'CCGG_w
Cuts at: 0 461 957
Size: 461 496
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 679 709 957
Size: 679 30 248
Fragments arranged by size:
679 248 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 182 957
Size: 182 775
BseYI C'CCAG_C
Cuts at: 0 467 957
Size: 467 490
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 867 903 957
Size: 377 490 36 54
Fragments arranged by size:
490 377 54 36
BsiHKAI G_wGCw'C
Cuts at: 0 272 957
Size: 272 685
BsmI GAATG_Cn'
Cuts at: 0 577 957
Size: 577 380
Bsp1286I G_dGCh'C
Cuts at: 0 272 325 957
Size: 272 53 632
Fragments arranged by size:
632 272 53
BspEI T'CCGG_A
Cuts at: 0 461 957
Size: 461 496
BspHI T'CATG_A
Cuts at: 0 231 957
Size: 231 726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 195 667 957
Size: 195 472 290
Fragments arranged by size:
472 290 195
BsrDI GCAATG_nn'
Cuts at: 0 156 957
Size: 156 801
BsrFI r'CCGG_y
Cuts at: 0 614 957
Size: 614 343
BstEII G'GTnAC_C
Cuts at: 0 413 957
Size: 413 544
BstYI r'GATC_y
Cuts at: 0 3 866 957
Size: 3 863 91
Fragments arranged by size:
863 91 3
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 856 957
Size: 856 101
BtsI GCAGTG_nn'
Cuts at: 0 30 387 575 625 690 753 957
Size: 30 357 188 50 65 63 204
Fragments arranged by size:
357 204 188 65 63 50 30
EarI CTCTTCn'nnn_
Cuts at: 0 393 957
Size: 393 564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 940 957
Size: 859 81 17
Fragments arranged by size:
859 81 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 827 957
Size: 827 130
EcoO109I rG'GnC_Cy
Cuts at: 0 166 321 322 516 587 957
Size: 166 155 1 194 71 370
Fragments arranged by size:
370 194 166 155 71 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 522 699 957
Size: 522 177 258
Fragments arranged by size:
522 258 177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 923 957
Size: 923 34
MslI CAynn'nnrTG
Cuts at: 0 23 957
Size: 23 934
NspI r_CATG'y
Cuts at: 0 298 666 957
Size: 298 368 291
Fragments arranged by size:
368 298 291
PasI CC'CwG_GG
Cuts at: 0 877 957
Size: 877 80
PflMI CCAn_nnn'nTGG
Cuts at: 0 436 957
Size: 436 521
PpuMI rG'GwC_Cy
Cuts at: 0 516 587 957
Size: 516 71 370
Fragments arranged by size:
516 370 71
PspOMI G'GGCC_C
Cuts at: 0 321 957
Size: 321 636
ScaI AGT'ACT
Cuts at: 0 302 957
Size: 302 655
SmaI CCC'GGG
Cuts at: 0 937 957
Size: 937 20
TatI w'GTAC_w
Cuts at: 0 300 957
Size: 300 657
XbaI T'CTAG_A
Cuts at: 0 763 957
Size: 763 194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 16 957
Size: 16 941
XmaI C'CCGG_G
Cuts at: 0 935 957
Size: 935 22
Enzymes that do cut:
AcuI AlwNI ApaI AseI AvaI BaeI BamHI BanII
Bme1580I BpmI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BstEII
BstYI BtgZI BtsI EarI Eco57MI EcoNI EcoO109I FalI
MmeI MslI NspI PasI PflMI PpuMI PspOMI ScaI
SmaI TatI XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AsiSI AvrII BanI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmBI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MscI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TstI Tth111I XhoI XmnI ZraI