Restriction map for
pFN21AE2204
(Linear) (Six-base) MAPSORT of: KIEE2204.seq Check: 224 from: 1 to: 2910
KIEE2204.orf
With 182 enzymes: SgfI *
December 5, 2014 12:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2229 2910
Size: 2229 681
Acc65I G'GTAC_C
Cuts at: 0 1861 2910
Size: 1861 1049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1342 1444 1646 1804 2910
Size: 1342 102 202 158 1106
Fragments arranged by size:
1342 1106 202 158 102
AflII C'TTAA_G
Cuts at: 0 153 2910
Size: 153 2757
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 544 1168 2910
Size: 544 624 1742
Fragments arranged by size:
1742 624 544
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2008 2040 2910
Size: 2008 32 870
Fragments arranged by size:
2008 870 32
AlwNI CAG_nnn'CTG
Cuts at: 0 438 1020 1113 1266 1332 1401 1608 1642
Size: 438 582 93 153 66 69 207 34
Cuts at: 1642 1896 1899 2013 2244 2910
Size: 254 3 114 231 666
Fragments arranged by size:
666 582 438 254 231 207 153 114
93 69 66 34 3
ApaI G_GGCC'C
Cuts at: 0 174 2307 2910
Size: 174 2133 603
Fragments arranged by size:
2133 603 174
ApaLI G'TGCA_C
Cuts at: 0 1810 2910
Size: 1810 1100
ApoI r'AATT_y
Cuts at: 0 1348 2017 2910
Size: 1348 669 893
Fragments arranged by size:
1348 893 669
AvaI C'yCGr_G
Cuts at: 0 312 1040 1562 2910
Size: 312 728 522 1348
Fragments arranged by size:
1348 728 522 312
AvrII C'CTAG_G
Cuts at: 0 822 2364 2910
Size: 822 1542 546
Fragments arranged by size:
1542 822 546
BamHI G'GATC_C
Cuts at: 0 2625 2802 2910
Size: 2625 177 108
Fragments arranged by size:
2625 177 108
BanI G'GyrC_C
Cuts at: 0 25 307 1861 2470 2544 2738 2910
Size: 25 282 1554 609 74 194 172
Fragments arranged by size:
1554 609 282 194 172 74 25
BanII G_rGCy'C
Cuts at: 0 174 748 2307 2635 2910
Size: 174 574 1559 328 275
Fragments arranged by size:
1559 574 328 275 174
BbeI G_GCGC'C
Cuts at: 0 2742 2910
Size: 2742 168
BbsI GAAGACnn'nnnn_
Cuts at: 0 77 275 815 1432 2910
Size: 77 198 540 617 1478
Fragments arranged by size:
1478 617 540 198 77
BbvCI CC'TCA_GC
Cuts at: 0 517 896 2455 2910
Size: 517 379 1559 455
Fragments arranged by size:
1559 517 455 379
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 283 317 2910
Size: 283 34 2593
Fragments arranged by size:
2593 283 34
BclI T'GATC_A
Cuts at: 0 1169 1508 2910
Size: 1169 339 1402
Fragments arranged by size:
1402 1169 339
BglI GCCn_nnn'nGGC
Cuts at: 0 752 1760 2177 2910
Size: 752 1008 417 733
Fragments arranged by size:
1008 752 733 417
BlpI GC'TnA_GC
Cuts at: 0 551 1922 2910
Size: 551 1371 988
Fragments arranged by size:
1371 988 551
Bme1580I G_kGCm'C
Cuts at: 0 174 1814 2307 2748 2910
Size: 174 1640 493 441 162
Fragments arranged by size:
1640 493 441 174 162
BmgBI CAC'GTC
Cuts at: 0 813 2910
Size: 813 2097
BmrI ACTGGGnnnn_n'
Cuts at: 0 152 714 2166 2910
Size: 152 562 1452 744
Fragments arranged by size:
1452 744 562 152
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 523 2910
Size: 523 2387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 580 589 682 1363 1946 2120 2788
Size: 344 236 9 93 681 583 174 668
Cuts at: 2788 2910
Size: 122
Fragments arranged by size:
681 668 583 344 236 174 122 93
9
Bpu10I CC'TnA_GC
Cuts at: 0 517 896 1750 2455 2910
Size: 517 379 854 705 455
Fragments arranged by size:
854 705 517 455 379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 2910
Size: 442 2468
BsaI GGTCTCn'nnnn_
Cuts at: 0 777 2910
Size: 777 2133
BsaAI yAC'GTr
Cuts at: 0 1275 1953 2910
Size: 1275 678 957
Fragments arranged by size:
1275 957 678
BsaBI GATnn'nnATC
Cuts at: 0 1693 2910
Size: 1693 1217
BsaHI Gr'CG_yC
Cuts at: 0 2739 2910
Size: 2739 171
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2093 2123 2486 2516 2761 2791 2910
Size: 2093 30 363 30 245 30 119
Fragments arranged by size:
2093 363 245 119 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 499 595 598 631 913 916 930 933
Size: 499 96 3 33 282 3 14 3
Cuts at: 933 2302 2844 2910
Size: 1369 542 66
Fragments arranged by size:
1369 542 499 282 96 66 33 14
3 3 3
BseYI C'CCAG_C
Cuts at: 0 710 1085 1238 1259 1582 2175 2910
Size: 710 375 153 21 323 593 735
Fragments arranged by size:
735 710 593 375 323 153 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 472 853 1504 2699 2910
Size: 373 99 381 651 1195 211
Fragments arranged by size:
1195 651 381 373 211 99
BsiEI CG_ry'CG
Cuts at: 0 849 2910
Size: 849 2061
BsiHKAI G_wGCw'C
Cuts at: 0 1780 1814 2574 2910
Size: 1780 34 760 336
Fragments arranged by size:
1780 760 336 34
BsmI GAATG_Cn'
Cuts at: 0 1840 2069 2910
Size: 1840 229 841
Fragments arranged by size:
1840 841 229
Bsp1286I G_dGCh'C
Cuts at: 0 174 748 1780 1814 2307 2574 2635 2748
Size: 174 574 1032 34 493 267 61 113
Cuts at: 2748 2910
Size: 162
Fragments arranged by size:
1032 574 493 267 174 162 113 61
34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2144 2229 2910
Size: 2144 85 681
Fragments arranged by size:
2144 681 85
BsrBI CCG'CTC
Cuts at: 0 555 2910
Size: 555 2355
BsrDI GCAATG_nn'
Cuts at: 0 1850 2667 2910
Size: 1850 817 243
Fragments arranged by size:
1850 817 243
BsrFI r'CCGG_y
Cuts at: 0 330 1872 2369 2617 2910
Size: 330 1542 497 248 293
Fragments arranged by size:
1542 497 330 293 248
BssSI C'ACGA_G
Cuts at: 0 877 958 2910
Size: 877 81 1952
Fragments arranged by size:
1952 877 81
BstAPI GCAn_nnn'nTGC
Cuts at: 0 438 450 1599 1608 1642 1896 2244 2910
Size: 438 12 1149 9 34 254 348 666
Fragments arranged by size:
1149 666 438 348 254 34 12 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2275 2910
Size: 2275 635
BstYI r'GATC_y
Cuts at: 0 1328 1997 2625 2802 2910
Size: 1328 669 628 177 108
Fragments arranged by size:
1328 669 628 177 108
Bsu36I CC'TnA_GG
Cuts at: 0 478 2910
Size: 478 2432
BtgI C'CryG_G
Cuts at: 0 1139 2910
Size: 1139 1771
BtsI GCAGTG_nn'
Cuts at: 0 1556 1850 2729 2910
Size: 1556 294 879 181
Fragments arranged by size:
1556 879 294 181
DraIII CAC_nnn'GTG
Cuts at: 0 450 1221 2232 2910
Size: 450 771 1011 678
Fragments arranged by size:
1011 771 678 450
DrdI GACnn_nn'nnGTC
Cuts at: 0 1741 2910
Size: 1741 1169
EaeI y'GGCC_r
Cuts at: 0 1124 1724 2277 2464 2607 2910
Size: 1124 600 553 187 143 303
Fragments arranged by size:
1124 600 553 303 187 143
EarI CTCTTCn'nnn_
Cuts at: 0 209 359 736 1450 1495 2713 2910
Size: 209 150 377 714 45 1218 197
Fragments arranged by size:
1218 714 377 209 197 150 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 409 502 2910
Size: 409 93 2408
Fragments arranged by size:
2408 409 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 580 589 682 1342 1363 1444 1646
Size: 344 236 9 93 660 21 81 202
Cuts at: 1646 1804 1946 2120 2788 2910
Size: 158 142 174 668 122
Fragments arranged by size:
668 660 344 236 202 174 158 142
122 93 81 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 481 994 1030 1477 2140 2910
Size: 481 513 36 447 663 770
Fragments arranged by size:
770 663 513 481 447 36
EcoO109I rG'GnC_Cy
Cuts at: 0 170 171 372 987 2303 2304 2859 2910
Size: 170 1 201 615 1316 1 555 51
Fragments arranged by size:
1316 615 555 201 170 51 1 1
EcoRI G'AATT_C
Cuts at: 0 2017 2910
Size: 2017 893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 765 2910
Size: 765 2145
HaeII r_GCGC'y
Cuts at: 0 477 2742 2910
Size: 477 2265 168
Fragments arranged by size:
2265 477 168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1614 1646 2093 2125 2910
Size: 1614 32 447 32 785
Fragments arranged by size:
1614 785 447 32 32
HincII GTy'rAC
Cuts at: 0 2518 2910
Size: 2518 392
KasI G'GCGC_C
Cuts at: 0 2738 2910
Size: 2738 172
KpnI G_GTAC'C
Cuts at: 0 1865 2910
Size: 1865 1045
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 679 2027 2304 2910
Size: 157 522 1348 277 606
Fragments arranged by size:
1348 606 522 277 157
MscI TGG'CCA
Cuts at: 0 2279 2609 2910
Size: 2279 330 301
Fragments arranged by size:
2279 330 301
MslI CAynn'nnrTG
Cuts at: 0 1210 2910
Size: 1210 1700
MspA1I CmG'CkG
Cuts at: 0 438 567 714 851 1017 1089 1141 1232
Size: 438 129 147 137 166 72 52 91
Cuts at: 1232 1263 1807 1896 1994 2013 2766 2910
Size: 31 544 89 98 19 753 144
Fragments arranged by size:
753 544 438 166 147 144 137 129
98 91 89 72 52 31 19
NaeI GCC'GGC
Cuts at: 0 2619 2910
Size: 2619 291
NarI GG'CG_CC
Cuts at: 0 2739 2910
Size: 2739 171
NgoMIV G'CCGG_C
Cuts at: 0 2617 2910
Size: 2617 293
NspI r_CATG'y
Cuts at: 0 864 1123 1215 2394 2910
Size: 864 259 92 1179 516
Fragments arranged by size:
1179 864 516 259 92
PasI CC'CwG_GG
Cuts at: 0 2477 2910
Size: 2477 433
PflMI CCAn_nnn'nTGG
Cuts at: 0 1092 2013 2735 2910
Size: 1092 921 722 175
Fragments arranged by size:
1092 921 722 175
PfoI T'CCnGG_A
Cuts at: 0 65 977 2097 2207 2574 2910
Size: 65 912 1120 110 367 336
Fragments arranged by size:
1120 912 367 336 110 65
PmlI CAC'GTG
Cuts at: 0 1953 2910
Size: 1953 957
PpuMI rG'GwC_Cy
Cuts at: 0 372 2859 2910
Size: 372 2487 51
Fragments arranged by size:
2487 372 51
PshAI GACnn'nnGTC
Cuts at: 0 1652 2910
Size: 1652 1258
PspOMI G'GGCC_C
Cuts at: 0 170 2303 2910
Size: 170 2133 607
Fragments arranged by size:
2133 607 170
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1300 1332 2910
Size: 1300 32 1578
Fragments arranged by size:
1578 1300 32
PstI C_TGCA'G
Cuts at: 0 306 434 443 1083 1157 1271 1604 1613
Size: 306 128 9 640 74 114 333 9
Cuts at: 1613 1647 1993 2418 2426 2910
Size: 34 346 425 8 484
Fragments arranged by size:
640 484 425 346 333 306 128 114
74 34 9 9 8
PvuII CAG'CTG
Cuts at: 0 438 567 714 1017 1089 1263 1807 1896
Size: 438 129 147 303 72 174 544 89
Cuts at: 1896 2910
Size: 1014
Fragments arranged by size:
1014 544 438 303 174 147 129 89
72
SacII CC_GC'GG
Cuts at: 0 1142 2910
Size: 1142 1768
SapI GCTCTTCn'nnn_
Cuts at: 0 736 1495 2910
Size: 736 759 1415
Fragments arranged by size:
1415 759 736
SexAI A'CCwGG_T
Cuts at: 0 374 1160 2228 2910
Size: 374 786 1068 682
Fragments arranged by size:
1068 786 682 374
SfcI C'TryA_G
Cuts at: 0 302 430 439 693 1079 1153 1267 1600
Size: 302 128 9 254 386 74 114 333
Cuts at: 1600 1609 1643 1989 2414 2422 2910
Size: 9 34 346 425 8 488
Fragments arranged by size:
488 425 386 346 333 302 254 128
114 74 34 9 9 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2177 2910
Size: 2177 733
SfoI GGC'GCC
Cuts at: 0 2740 2910
Size: 2740 170
SmaI CCC'GGG
Cuts at: 0 1042 1564 2910
Size: 1042 522 1346
Fragments arranged by size:
1346 1042 522
SmlI C'TyrA_G
Cuts at: 0 153 457 2910
Size: 153 304 2453
Fragments arranged by size:
2453 304 153
SphI G_CATG'C
Cuts at: 0 1123 1215 2394 2910
Size: 1123 92 1179 516
Fragments arranged by size:
1179 1123 516 92
SrfI GCCC'GGGC
Cuts at: 0 1042 2910
Size: 1042 1868
StuI AGG'CCT
Cuts at: 0 318 2272 2910
Size: 318 1954 638
Fragments arranged by size:
1954 638 318
StyI C'CwwG_G
Cuts at: 0 777 822 1026 1698 1758 2364 2910
Size: 777 45 204 672 60 606 546
Fragments arranged by size:
777 672 606 546 204 60 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 398 2910
Size: 398 2512
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2486 2518 2910
Size: 2486 32 392
Fragments arranged by size:
2486 392 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2010 2910
Size: 2010 900
XmaI C'CCGG_G
Cuts at: 0 1040 1562 2910
Size: 1040 522 1348
Fragments arranged by size:
1348 1040 522
XmnI GAAnn'nnTTC
Cuts at: 0 2203 2910
Size: 2203 707
Enzymes that do cut:
AarI Acc65I AcuI AflII AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PsrI PstI PvuII SacII SapI SexAI
SfcI SfiI SfoI SmaI SmlI SphI SrfI StuI
StyI TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflIII AgeI AhdI AleI
AscI AseI AsiSI BaeI BciVI BfrBI BglII BmtI
BsaWI BsiWI BsmBI BspEI BspHI BsrGI BssHII BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI EagI EcoICRI
EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PpiI PsiI PspXI PvuI RsrII SacI SalI
SanDI SbfI ScaI SgfI SgrAI SnaBI SpeI SspI
SwaI TatI Tth111I XbaI XhoI ZraI