Restriction map for
pFN21AE2203
(Linear) (Six-base) MAPSORT of: KIEE2203.seq Check: 1954 from: 1 to: 2625
KIEE2203.orf
With 182 enzymes: SgfI *
July 3, 2012 17:13 ..
AccI GT'mk_AC
Cuts at: 0 1041 2625
Size: 1041 1584
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 955 1147 1276 1522 2170 2432 2625
Size: 142 813 192 129 246 648 262 193
Fragments arranged by size:
813 648 262 246 193 192 142 129
AflIII A'CryG_T
Cuts at: 0 1524 2625
Size: 1524 1101
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2132 2625
Size: 2132 493
AlwNI CAG_nnn'CTG
Cuts at: 0 984 2625
Size: 984 1641
ApaLI G'TGCA_C
Cuts at: 0 585 2625
Size: 585 2040
ApoI r'AATT_y
Cuts at: 0 1639 2040 2200 2625
Size: 1639 401 160 425
Fragments arranged by size:
1639 425 401 160
BanI G'GyrC_C
Cuts at: 0 1518 2625
Size: 1518 1107
BanII G_rGCy'C
Cuts at: 0 36 1404 1611 2625
Size: 36 1368 207 1014
Fragments arranged by size:
1368 1014 207 36
BbsI GAAGACnn'nnnn_
Cuts at: 0 1763 2507 2625
Size: 1763 744 118
Fragments arranged by size:
1763 744 118
BbvCI CC'TCA_GC
Cuts at: 0 2132 2625
Size: 2132 493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 980 1014 2625
Size: 980 34 1611
Fragments arranged by size:
1611 980 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2556 2625
Size: 2556 69
Bme1580I G_kGCm'C
Cuts at: 0 589 2625
Size: 589 2036
BmgBI CAC'GTC
Cuts at: 0 2505 2625
Size: 2505 120
Bpu10I CC'TnA_GC
Cuts at: 0 2132 2625
Size: 2132 493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 816 1361 1790 2625
Size: 816 545 429 835
Fragments arranged by size:
835 816 545 429
BsaI GGTCTCn'nnnn_
Cuts at: 0 523 2625
Size: 523 2102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2475 2505 2625
Size: 2475 30 120
Fragments arranged by size:
2475 120 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1897 2496 2625
Size: 1897 599 129
Fragments arranged by size:
1897 599 129
BseYI C'CCAG_C
Cuts at: 0 423 2625
Size: 423 2202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1300 2625
Size: 1300 1325
BsiEI CG_ry'CG
Cuts at: 0 1148 2625
Size: 1148 1477
BsiHKAI G_wGCw'C
Cuts at: 0 589 1404 1712 2625
Size: 589 815 308 913
Fragments arranged by size:
913 815 589 308
BsmI GAATG_Cn'
Cuts at: 0 714 2625
Size: 714 1911
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1422 2625
Size: 1422 1203
Bsp1286I G_dGCh'C
Cuts at: 0 36 589 1404 1611 1712 2625
Size: 36 553 815 207 101 913
Fragments arranged by size:
913 815 553 207 101 36
BspHI T'CATG_A
Cuts at: 0 501 828 2625
Size: 501 327 1797
Fragments arranged by size:
1797 501 327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 129 1198 2625
Size: 129 1069 1427
Fragments arranged by size:
1427 1069 129
BsrBI CCG'CTC
Cuts at: 0 334 2625
Size: 334 2291
BsrDI GCAATG_nn'
Cuts at: 0 409 2625
Size: 409 2216
BsrFI r'CCGG_y
Cuts at: 0 1001 2566 2625
Size: 1001 1565 59
Fragments arranged by size:
1565 1001 59
BssSI C'ACGA_G
Cuts at: 0 580 2625
Size: 580 2045
BstXI CCAn_nnnn'nTGG
Cuts at: 0 303 1134 1569 2625
Size: 303 831 435 1056
Fragments arranged by size:
1056 831 435 303
BstYI r'GATC_y
Cuts at: 0 346 537 1801 2388 2625
Size: 346 191 1264 587 237
Fragments arranged by size:
1264 587 346 237 191
Bsu36I CC'TnA_GG
Cuts at: 0 1178 2625
Size: 1178 1447
BtgI C'CryG_G
Cuts at: 0 1035 2625
Size: 1035 1590
BtsI GCAGTG_nn'
Cuts at: 0 2101 2625
Size: 2101 524
DraI TTT'AAA
Cuts at: 0 1391 2046 2625
Size: 1391 655 579
Fragments arranged by size:
1391 655 579
DrdI GACnn_nn'nnGTC
Cuts at: 0 2516 2625
Size: 2516 109
EaeI y'GGCC_r
Cuts at: 0 11 335 1145 2098 2625
Size: 11 324 810 953 527
Fragments arranged by size:
953 810 527 324 11
EagI C'GGCC_G
Cuts at: 0 1145 2625
Size: 1145 1480
EarI CTCTTCn'nnn_
Cuts at: 0 937 1772 1823 2029 2186 2271 2309 2473
Size: 937 835 51 206 157 85 38 164
Cuts at: 2473 2585 2625
Size: 112 40
Fragments arranged by size:
937 835 206 164 157 112 85 51
40 38
EcoICRI GAG'CTC
Cuts at: 0 1402 2625
Size: 1402 1223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 955 1147 1276 1522 2170 2432 2625
Size: 142 813 192 129 246 648 262 193
Fragments arranged by size:
813 648 262 246 193 192 142 129
EcoO109I rG'GnC_Cy
Cuts at: 0 783 2625
Size: 783 1842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 343 2625
Size: 343 2282
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 45 77 915 947 1651 1683 2625
Size: 45 32 838 32 704 32 942
Fragments arranged by size:
942 838 704 45 32 32 32
HincII GTy'rAC
Cuts at: 0 1042 2625
Size: 1042 1583
HindIII A'AGCT_T
Cuts at: 0 1241 2625
Size: 1241 1384
MluI A'CGCG_T
Cuts at: 0 1524 2625
Size: 1524 1101
MscI TGG'CCA
Cuts at: 0 2100 2625
Size: 2100 525
MslI CAynn'nnrTG
Cuts at: 0 614 2625
Size: 614 2011
MspA1I CmG'CkG
Cuts at: 0 514 2625
Size: 514 2111
NaeI GCC'GGC
Cuts at: 0 1003 2625
Size: 1003 1622
NcoI C'CATG_G
Cuts at: 0 1035 2625
Size: 1035 1590
NgoMIV G'CCGG_C
Cuts at: 0 1001 2625
Size: 1001 1624
PpuMI rG'GwC_Cy
Cuts at: 0 783 2625
Size: 783 1842
PstI C_TGCA'G
Cuts at: 0 893 1101 2625
Size: 893 208 1524
Fragments arranged by size:
1524 893 208
PvuII CAG'CTG
Cuts at: 0 514 2625
Size: 514 2111
SacI G_AGCT'C
Cuts at: 0 1404 2625
Size: 1404 1221
SalI G'TCGA_C
Cuts at: 0 1040 2625
Size: 1040 1585
SapI GCTCTTCn'nnn_
Cuts at: 0 937 2585 2625
Size: 937 1648 40
Fragments arranged by size:
1648 937 40
ScaI AGT'ACT
Cuts at: 0 1103 2625
Size: 1103 1522
SexAI A'CCwGG_T
Cuts at: 0 591 2625
Size: 591 2034
SfcI C'TryA_G
Cuts at: 0 889 1097 2625
Size: 889 208 1528
Fragments arranged by size:
1528 889 208
SmlI C'TyrA_G
Cuts at: 0 795 1376 1805 2625
Size: 795 581 429 820
Fragments arranged by size:
820 795 581 429
StyI C'CwwG_G
Cuts at: 0 1035 1603 2625
Size: 1035 568 1022
Fragments arranged by size:
1035 1022 568
TatI w'GTAC_w
Cuts at: 0 324 1101 2625
Size: 324 777 1524
Fragments arranged by size:
1524 777 324
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2475 2507 2625
Size: 2475 32 118
Fragments arranged by size:
2475 118 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1566 1629 2625
Size: 1566 63 996
Fragments arranged by size:
1566 996 63
XmnI GAAnn'nnTTC
Cuts at: 0 2183 2324 2625
Size: 2183 141 301
Fragments arranged by size:
2183 301 141
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApaLI ApoI BanI
BanII BbsI BbvCI BcgI BciVI Bme1580I BmgBI Bpu10I
BpuEI BsaI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstXI BstYI Bsu36I BtgI BtsI DraI DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I
HincII HindIII MluI MscI MslI MspA1I NaeI NcoI
NgoMIV PpuMI PstI PvuII SacI SalI SapI ScaI
SexAI SfcI SmlI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BclI BfrBI BglI BglII BlpI
BmrI BmtI BplI BpmI BsaAI BsaBI BsaHI BsaWI
BsiWI BspEI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraIII EciI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MmeI NarI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XhoI XmaI ZraI