Restriction map for
pFN21AE2201
(Linear) (Six-base) MAPSORT of: KIEE2201.seq Check: 8335 from: 1 to: 2133
KIEE2201.orf
With 182 enzymes: SgfI *
November 2, 2012 16:48 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 633 2078 2133
Size: 633 1445 55
Fragments arranged by size:
1445 633 55
AleI CACnn'nnGTG
Cuts at: 0 283 1304 2133
Size: 283 1021 829
Fragments arranged by size:
1021 829 283
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 994 1685 2133
Size: 994 691 448
Fragments arranged by size:
994 691 448
AlwNI CAG_nnn'CTG
Cuts at: 0 598 1531 2083 2133
Size: 598 933 552 50
Fragments arranged by size:
933 598 552 50
ApaI G_GGCC'C
Cuts at: 0 100 172 243 655 1097 1124 1550 1565
Size: 100 72 71 412 442 27 426 15
Cuts at: 1565 1610 1768 2133
Size: 45 158 365
Fragments arranged by size:
442 426 412 365 158 100 72 71
45 27 15
AvaI C'yCGr_G
Cuts at: 0 31 147 198 562 638 1096 1221 1582
Size: 31 116 51 364 76 458 125 361
Cuts at: 1582 1761 2133
Size: 179 372
Fragments arranged by size:
458 372 364 361 179 125 116 76
51 31
BamHI G'GATC_C
Cuts at: 0 861 2133
Size: 861 1272
BanI G'GyrC_C
Cuts at: 0 73 697 2133
Size: 73 624 1436
Fragments arranged by size:
1436 624 73
BanII G_rGCy'C
Cuts at: 0 100 172 243 591 655 1097 1124 1550
Size: 100 72 71 348 64 442 27 426
Cuts at: 1550 1565 1590 1610 1768 1820 1998 2133
Size: 15 25 20 158 52 178 135
Fragments arranged by size:
442 426 348 178 158 135 100 72
71 64 52 27 25 20 15
BbeI G_GCGC'C
Cuts at: 0 77 2133
Size: 77 2056
BbvCI CC'TCA_GC
Cuts at: 0 1823 2133
Size: 1823 310
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1087 1121 2133
Size: 1087 34 1012
Fragments arranged by size:
1087 1012 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1058 2133
Size: 1058 1075
BclI T'GATC_A
Cuts at: 0 473 1031 2133
Size: 473 558 1102
Fragments arranged by size:
1102 558 473
BglI GCCn_nnn'nGGC
Cuts at: 0 104 695 1328 1520 1535 1726 1795 1804
Size: 104 591 633 192 15 191 69 9
Cuts at: 1804 2032 2133
Size: 228 101
Fragments arranged by size:
633 591 228 192 191 104 101 69
15 9
BglII A'GATC_T
Cuts at: 0 1376 2133
Size: 1376 757
Bme1580I G_kGCm'C
Cuts at: 0 100 172 243 655 989 1097 1124 1550
Size: 100 72 71 412 334 108 27 426
Cuts at: 1550 1565 1610 1768 2133
Size: 15 45 158 365
Fragments arranged by size:
426 412 365 334 158 108 100 72
71 45 27 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 1495 1650 2133
Size: 1495 155 483
Fragments arranged by size:
1495 483 155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 809 1153 1184 2133
Size: 527 282 344 31 949
Fragments arranged by size:
949 527 344 282 31
Bpu10I CC'TnA_GC
Cuts at: 0 1808 1823 2133
Size: 1808 15 310
Fragments arranged by size:
1808 310 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 754 2133
Size: 754 1379
BsaBI GATnn'nnATC
Cuts at: 0 344 715 901 2133
Size: 344 371 186 1232
Fragments arranged by size:
1232 371 344 186
BsaHI Gr'CG_yC
Cuts at: 0 74 227 2133
Size: 74 153 1906
Fragments arranged by size:
1906 153 74
BsaWI w'CCGG_w
Cuts at: 0 351 938 1978 2133
Size: 351 587 1040 155
Fragments arranged by size:
1040 587 351 155
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1355 1385 2133
Size: 1355 30 748
Fragments arranged by size:
1355 748 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 500 1156 1604 1664 1960 2133
Size: 500 656 448 60 296 173
Fragments arranged by size:
656 500 448 296 173 60
BseYI C'CCAG_C
Cuts at: 0 1442 1637 1898 2009 2093 2133
Size: 1442 195 261 111 84 40
Fragments arranged by size:
1442 261 195 111 84 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 724 895 2133
Size: 253 471 171 1238
Fragments arranged by size:
1238 471 253 171
BsiEI CG_ry'CG
Cuts at: 0 164 1323 1706 2133
Size: 164 1159 383 427
Fragments arranged by size:
1159 427 383 164
BsiHKAI G_wGCw'C
Cuts at: 0 1142 1998 2133
Size: 1142 856 135
Fragments arranged by size:
1142 856 135
BsmI GAATG_Cn'
Cuts at: 0 993 2133
Size: 993 1140
Bsp1286I G_dGCh'C
Cuts at: 0 100 172 243 591 655 989 1097 1124
Size: 100 72 71 348 64 334 108 27
Cuts at: 1124 1142 1550 1565 1590 1610 1768 1820 1998
Size: 18 408 15 25 20 158 52 178
Cuts at: 1998 2133
Size: 135
Fragments arranged by size:
408 348 334 178 158 135 108 100
72 71 64 52 27 25 20 18
15
BspHI T'CATG_A
Cuts at: 0 713 1677 2133
Size: 713 964 456
Fragments arranged by size:
964 713 456
BsrFI r'CCGG_y
Cuts at: 0 49 733 1785 1803 1837 2133
Size: 49 684 1052 18 34 296
Fragments arranged by size:
1052 684 296 49 34 18
BstYI r'GATC_y
Cuts at: 0 817 861 952 1040 1376 2133
Size: 817 44 91 88 336 757
Fragments arranged by size:
817 757 336 91 88 44
Bsu36I CC'TnA_GG
Cuts at: 0 1177 2133
Size: 1177 956
BtgI C'CryG_G
Cuts at: 0 722 1706 2133
Size: 722 984 427
Fragments arranged by size:
984 722 427
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 284 2133
Size: 284 1849
BtsI GCAGTG_nn'
Cuts at: 0 576 2133
Size: 576 1557
ClaI AT'CG_AT
Cuts at: 0 974 2133
Size: 974 1159
EaeI y'GGCC_r
Cuts at: 0 161 1014 1320 1703 1839 1887 1973 2133
Size: 161 853 306 383 136 48 86 160
Fragments arranged by size:
853 383 306 161 160 136 86 48
EagI C'GGCC_G
Cuts at: 0 161 1320 1703 2133
Size: 161 1159 383 430
Fragments arranged by size:
1159 430 383 161
EarI CTCTTCn'nnn_
Cuts at: 0 434 1236 1862 2133
Size: 434 802 626 271
Fragments arranged by size:
802 626 434 271
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 80 203 980 2050 2133
Size: 80 123 777 1070 83
Fragments arranged by size:
1070 777 123 83 80
EcoICRI GAG'CTC
Cuts at: 0 1996 2133
Size: 1996 137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 809 1153 1184 2133
Size: 527 282 344 31 949
Fragments arranged by size:
949 527 344 282 31
EcoO109I rG'GnC_Cy
Cuts at: 0 22 85 168 169 450 651 652 1093
Size: 22 63 83 1 281 201 1 441
Cuts at: 1093 1120 1121 1195 1261 1404 1489 1528 1546
Size: 27 1 74 66 143 85 39 18
Cuts at: 1546 1547 1562 1606 1607 1765 2080 2133
Size: 1 15 44 1 158 315 53
Fragments arranged by size:
441 315 281 201 158 143 85 83
74 66 63 53 44 39 27 22
18 15 1 1 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 819 2133
Size: 819 1314
FseI GG_CCGG'CC
Cuts at: 0 737 2133
Size: 737 1396
HaeII r_GCGC'y
Cuts at: 0 77 1915 2133
Size: 77 1838 218
Fragments arranged by size:
1838 218 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 371 403 619 651 937 969 1016 1048
Size: 371 32 216 32 286 32 47 32
Cuts at: 1048 1473 1505 2064 2096 2133
Size: 425 32 559 32 37
Fragments arranged by size:
559 425 371 286 216 47 37 32
32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 73 2133
Size: 73 2060
MfeI C'AATT_G
Cuts at: 0 1254 2133
Size: 1254 879
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 534 1485 1826 2133
Size: 314 220 951 341 307
Fragments arranged by size:
951 341 314 307 220
MscI TGG'CCA
Cuts at: 0 1975 2133
Size: 1975 158
MslI CAynn'nnrTG
Cuts at: 0 283 976 1304 2133
Size: 283 693 328 829
Fragments arranged by size:
829 693 328 283
MspA1I CmG'CkG
Cuts at: 0 237 551 724 774 1389 1696 1702 1708
Size: 237 314 173 50 615 307 6 6
Cuts at: 1708 2133
Size: 425
Fragments arranged by size:
615 425 314 307 237 173 50 6
6
NaeI GCC'GGC
Cuts at: 0 51 735 2133
Size: 51 684 1398
Fragments arranged by size:
1398 684 51
NarI GG'CG_CC
Cuts at: 0 74 2133
Size: 74 2059
NgoMIV G'CCGG_C
Cuts at: 0 49 733 2133
Size: 49 684 1400
Fragments arranged by size:
1400 684 49
NotI GC'GGCC_GC
Cuts at: 0 1703 2133
Size: 1703 430
NspI r_CATG'y
Cuts at: 0 1218 1600 2133
Size: 1218 382 533
Fragments arranged by size:
1218 533 382
PasI CC'CwG_GG
Cuts at: 0 375 1632 2133
Size: 375 1257 501
Fragments arranged by size:
1257 501 375
PflMI CCAn_nnn'nTGG
Cuts at: 0 2083 2133
Size: 2083 50
PfoI T'CCnGG_A
Cuts at: 0 908 1989 2133
Size: 908 1081 144
Fragments arranged by size:
1081 908 144
PpuMI rG'GwC_Cy
Cuts at: 0 22 450 1195 1261 1404 1489 2080 2133
Size: 22 428 745 66 143 85 591 53
Fragments arranged by size:
745 591 428 143 85 66 53 22
PspOMI G'GGCC_C
Cuts at: 0 96 168 239 651 1093 1120 1546 1561
Size: 96 72 71 412 442 27 426 15
Cuts at: 1561 1606 1764 2133
Size: 45 158 369
Fragments arranged by size:
442 426 412 369 158 96 72 71
45 27 15
PstI C_TGCA'G
Cuts at: 0 779 1701 2133
Size: 779 922 432
Fragments arranged by size:
922 779 432
PvuII CAG'CTG
Cuts at: 0 774 1389 1696 2133
Size: 774 615 307 437
Fragments arranged by size:
774 615 437 307
SacI G_AGCT'C
Cuts at: 0 1998 2133
Size: 1998 135
SacII CC_GC'GG
Cuts at: 0 725 1709 2133
Size: 725 984 424
Fragments arranged by size:
984 725 424
SanDI GG'GwC_CC
Cuts at: 0 450 1261 1404 2133
Size: 450 811 143 729
Fragments arranged by size:
811 729 450 143
ScaI AGT'ACT
Cuts at: 0 1951 2133
Size: 1951 182
SfcI C'TryA_G
Cuts at: 0 9 775 1697 2019 2133
Size: 9 766 922 322 114
Fragments arranged by size:
922 766 322 114 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1520 1535 1795 2133
Size: 1520 15 260 338
Fragments arranged by size:
1520 338 260 15
SfoI GGC'GCC
Cuts at: 0 75 2133
Size: 75 2058
SgrAI Cr'CCGG_yG
Cuts at: 0 49 2133
Size: 49 2084
SmaI CCC'GGG
Cuts at: 0 33 149 200 1223 1584 1763 2133
Size: 33 116 51 1023 361 179 370
Fragments arranged by size:
1023 370 361 179 116 51 33
SphI G_CATG'C
Cuts at: 0 1218 2133
Size: 1218 915
SrfI GCCC'GGGC
Cuts at: 0 33 149 2133
Size: 33 116 1984
Fragments arranged by size:
1984 116 33
SspI AAT'ATT
Cuts at: 0 2041 2133
Size: 2041 92
StuI AGG'CCT
Cuts at: 0 1861 2133
Size: 1861 272
StyI C'CwwG_G
Cuts at: 0 421 921 1248 2133
Size: 421 500 327 885
Fragments arranged by size:
885 500 421 327
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1410 1848 2133
Size: 1410 438 285
Fragments arranged by size:
1410 438 285
TatI w'GTAC_w
Cuts at: 0 443 531 1949 2133
Size: 443 88 1418 184
Fragments arranged by size:
1418 443 184 88
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 618 928 1051 1634 2133
Size: 618 310 123 583 499
Fragments arranged by size:
618 583 499 310 123
XmaI C'CCGG_G
Cuts at: 0 31 147 198 1221 1582 1761 2133
Size: 31 116 51 1023 361 179 372
Fragments arranged by size:
1023 372 361 179 116 51 31
Enzymes that do cut:
AhdI AleI AlfI AlwNI ApaI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
Bme1580I BmrI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BsrFI BstYI Bsu36I BtgI BtgZI BtsI ClaI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI
FseI HaeII Hin4I KasI MfeI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NotI NspI PasI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI
ScaI SfcI SfiI SfoI SgrAI SmaI SphI SrfI
SspI StuI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbsI BfrBI BlpI BmgBI BmtI BplI
BpuEI BsaAI BsiWI BsmBI BspEI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FspI
FspAI HincII HindIII HpaI KpnI MluI NcoI NdeI
NheI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI
SbfI SexAI SgfI SmlI SnaBI SpeI SwaI TstI
Tth111I XbaI XhoI XmnI ZraI