(Linear) (Six-base) MAPSORT of: KIEE2199.seq  Check: 5254  from: 1  to: 279



KIEE2199.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 15:58  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      90     279 
   Size:         90     189
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     236     279 
   Size:        236      43
 
ApoI r'AATT_y

Cuts at:      0     135     279 
   Size:        135     144
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     176     224     279 
   Size:        176      48      55

  Fragments arranged by size: 

                 176      55      48
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      12     279 
   Size:         12     267
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22     250     279 
   Size:         22     228      29

  Fragments arranged by size: 

                 228      29      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      21      53     279 
   Size:         21      32     226

  Fragments arranged by size: 

                 226      32      21
 
BsiEI CG_ry'CG

Cuts at:      0      95     279 
   Size:         95     184
 
BsiHKAI G_wGCw'C

Cuts at:      0     175     279 
   Size:        175     104
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     151     279 
   Size:        151     128
 
Bsp1286I G_dGCh'C

Cuts at:      0     175     279 
   Size:        175     104
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     204     279 
   Size:        204      75
 
BsrFI r'CCGG_y

Cuts at:      0     209     279 
   Size:        209      70
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      76     170     279 
   Size:         76      94     109

  Fragments arranged by size: 

                 109      94      76
 
BtgI C'CryG_G

Cuts at:      0      41     279 
   Size:         41     238
 
BtsI GCAGTG_nn'

Cuts at:      0      11     279 
   Size:         11     268
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      90     279 
   Size:         90     189
 
EcoO109I rG'GnC_Cy

Cuts at:      0      60     279 
   Size:         60     219
 
NaeI GCC'GGC

Cuts at:      0     211     279 
   Size:        211      68
 
NcoI C'CATG_G

Cuts at:      0      41     279 
   Size:         41     238
 
NgoMIV G'CCGG_C

Cuts at:      0     209     279 
   Size:        209      70
 
PpuMI rG'GwC_Cy

Cuts at:      0      60     279 
   Size:         60     219
 
SexAI A'CCwGG_T

Cuts at:      0      56     279 
   Size:         56     223
 
StyI C'CwwG_G

Cuts at:      0      41     279 
   Size:         41     238
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     BmrI     BsaI    BseRI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrFI   BstAPI     BtgI     BtsI
  Eco57MI EcoO109I     NaeI     NcoI   NgoMIV    PpuMI    SexAI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiWI
     BsmI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NarI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI