Restriction map for
pFN21AE2195
(Linear) (Six-base) MAPSORT of: KIEE2195.seq Check: 4298 from: 1 to: 1254
KIEE2195.orf
With 182 enzymes: SgfI *
January 29, 2014 11:45 ..
AflIII A'CryG_T
Cuts at: 0 511 1254
Size: 511 743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 509 1254
Size: 509 745
AlwNI CAG_nnn'CTG
Cuts at: 0 127 1254
Size: 127 1127
AseI AT'TA_AT
Cuts at: 0 1105 1217 1254
Size: 1105 112 37
Fragments arranged by size:
1105 112 37
AvaI C'yCGr_G
Cuts at: 0 11 79 610 1254
Size: 11 68 531 644
Fragments arranged by size:
644 531 68 11
BamHI G'GATC_C
Cuts at: 0 1023 1254
Size: 1023 231
BanI G'GyrC_C
Cuts at: 0 75 233 480 1254
Size: 75 158 247 774
Fragments arranged by size:
774 247 158 75
BanII G_rGCy'C
Cuts at: 0 425 716 1254
Size: 425 291 538
Fragments arranged by size:
538 425 291
BbeI G_GCGC'C
Cuts at: 0 79 237 484 1254
Size: 79 158 247 770
Fragments arranged by size:
770 247 158 79
BbsI GAAGACnn'nnnn_
Cuts at: 0 514 1095 1254
Size: 514 581 159
Fragments arranged by size:
581 514 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 153 187 378 412 1052 1086 1254
Size: 153 34 191 34 640 34 168
Fragments arranged by size:
640 191 168 153 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 478 1254
Size: 478 776
BglII A'GATC_T
Cuts at: 0 1016 1254
Size: 1016 238
Bme1580I G_kGCm'C
Cuts at: 0 813 1254
Size: 813 441
BmgBI CAC'GTC
Cuts at: 0 617 1254
Size: 617 637
BmrI ACTGGGnnnn_n'
Cuts at: 0 681 807 1254
Size: 681 126 447
Fragments arranged by size:
681 447 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 1254
Size: 104 1150
BsaI GGTCTCn'nnnn_
Cuts at: 0 1 890 1254
Size: 1 889 364
Fragments arranged by size:
889 364 1
BsaHI Gr'CG_yC
Cuts at: 0 76 234 481 1254
Size: 76 158 247 773
Fragments arranged by size:
773 247 158 76
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 102 132 1254
Size: 102 30 1122
Fragments arranged by size:
1122 102 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 417 1254
Size: 417 837
BseYI C'CCAG_C
Cuts at: 0 579 1254
Size: 579 675
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 372 1130 1254
Size: 24 348 758 124
Fragments arranged by size:
758 348 124 24
BsiEI CG_ry'CG
Cuts at: 0 326 833 1254
Size: 326 507 421
Fragments arranged by size:
507 421 326
BsiHKAI G_wGCw'C
Cuts at: 0 365 557 941 1254
Size: 365 192 384 313
Fragments arranged by size:
384 365 313 192
BsmI GAATG_Cn'
Cuts at: 0 976 1254
Size: 976 278
BsmBI CGTCTCn'nnnn_
Cuts at: 0 829 1254
Size: 829 425
Bsp1286I G_dGCh'C
Cuts at: 0 365 425 557 716 813 941 1254
Size: 365 60 132 159 97 128 313
Fragments arranged by size:
365 313 159 132 128 97 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1046 1254
Size: 1046 208
BsrBI CCG'CTC
Cuts at: 0 462 891 1254
Size: 462 429 363
Fragments arranged by size:
462 429 363
BsrFI r'CCGG_y
Cuts at: 0 230 573 1254
Size: 230 343 681
Fragments arranged by size:
681 343 230
BsrGI T'GTAC_A
Cuts at: 0 1223 1254
Size: 1223 31
BssSI C'ACGA_G
Cuts at: 0 214 1204 1254
Size: 214 990 50
Fragments arranged by size:
990 214 50
BstBI TT'CG_AA
Cuts at: 0 1062 1254
Size: 1062 192
BstXI CCAn_nnnn'nTGG
Cuts at: 0 450 1254
Size: 450 804
BstYI r'GATC_y
Cuts at: 0 1016 1023 1052 1254
Size: 1016 7 29 202
Fragments arranged by size:
1016 202 29 7
BtgI C'CryG_G
Cuts at: 0 152 449 791 1254
Size: 152 297 342 463
Fragments arranged by size:
463 342 297 152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 423 1254
Size: 423 831
BtsI GCAGTG_nn'
Cuts at: 0 939 1254
Size: 939 315
DraIII CAC_nnn'GTG
Cuts at: 0 1153 1254
Size: 1153 101
EaeI y'GGCC_r
Cuts at: 0 203 452 470 548 749 839 1254
Size: 203 249 18 78 201 90 415
Fragments arranged by size:
415 249 203 201 90 78 18
EarI CTCTTCn'nnn_
Cuts at: 0 666 1254
Size: 666 588
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 78 1254
Size: 78 1176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 1254
Size: 104 1150
EcoO109I rG'GnC_Cy
Cuts at: 0 762 1254
Size: 762 492
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 276 1254
Size: 276 978
HaeII r_GCGC'y
Cuts at: 0 79 237 289 449 469 484 724 1254
Size: 79 158 52 160 20 15 240 530
Fragments arranged by size:
530 240 160 158 79 52 20 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 906 938 1008 1040 1254
Size: 127 32 747 32 70 32 214
Fragments arranged by size:
747 214 127 70 32 32 32
KasI G'GCGC_C
Cuts at: 0 75 233 480 1254
Size: 75 158 247 774
Fragments arranged by size:
774 247 158 75
MluI A'CGCG_T
Cuts at: 0 511 1254
Size: 511 743
MscI TGG'CCA
Cuts at: 0 205 472 751 1254
Size: 205 267 279 503
Fragments arranged by size:
503 279 267 205
MslI CAynn'nnrTG
Cuts at: 0 67 334 491 865 1219 1254
Size: 67 267 157 374 354 35
Fragments arranged by size:
374 354 267 157 67 35
MspA1I CmG'CkG
Cuts at: 0 776 1254
Size: 776 478
NarI GG'CG_CC
Cuts at: 0 76 234 481 1254
Size: 76 158 247 773
Fragments arranged by size:
773 247 158 76
NcoI C'CATG_G
Cuts at: 0 449 1254
Size: 449 805
NspI r_CATG'y
Cuts at: 0 72 1254
Size: 72 1182
PflMI CCAn_nnn'nTGG
Cuts at: 0 127 1020 1254
Size: 127 893 234
Fragments arranged by size:
893 234 127
PstI C_TGCA'G
Cuts at: 0 1114 1254
Size: 1114 140
SapI GCTCTTCn'nnn_
Cuts at: 0 666 1254
Size: 666 588
SexAI A'CCwGG_T
Cuts at: 0 296 1254
Size: 296 958
SfcI C'TryA_G
Cuts at: 0 1110 1254
Size: 1110 144
SfoI GGC'GCC
Cuts at: 0 77 235 482 1254
Size: 77 158 247 772
Fragments arranged by size:
772 247 158 77
SmaI CCC'GGG
Cuts at: 0 13 612 1254
Size: 13 599 642
Fragments arranged by size:
642 599 13
StyI C'CwwG_G
Cuts at: 0 449 785 1027 1254
Size: 449 336 242 227
Fragments arranged by size:
449 336 242 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 847 864 1254
Size: 847 17 390
Fragments arranged by size:
847 390 17
TatI w'GTAC_w
Cuts at: 0 593 1161 1223 1254
Size: 593 568 62 31
Fragments arranged by size:
593 568 62 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 102 134 1254
Size: 102 32 1120
Fragments arranged by size:
1120 102 32
Tth111I GACn'n_nGTC
Cuts at: 0 298 1254
Size: 298 956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 762 849 1254
Size: 762 87 405
Fragments arranged by size:
762 405 87
XmaI C'CCGG_G
Cuts at: 0 11 610 1254
Size: 11 599 644
Fragments arranged by size:
644 599 11
XmnI GAAnn'nnTTC
Cuts at: 0 259 1254
Size: 259 995
Enzymes that do cut:
AflIII AlfI AlwNI AseI AvaI BamHI BanI BanII
BbeI BbsI BcgI BglI BglII Bme1580I BmgBI BmrI
BpmI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI
BssSI BstBI BstXI BstYI BtgI BtgZI BtsI DraIII
EaeI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I
KasI MluI MscI MslI MspA1I NarI NcoI NspI
PflMI PstI SapI SexAI SfcI SfoI SmaI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI BlpI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsiWI
BspEI BspHI BsrDI BssHII BstAPI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MmeI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XhoI ZraI