Restriction map for
pFN21AE2192
(Linear) (Six-base) MAPSORT of: KIEE2192.seq Check: 8786 from: 1 to: 1722
KIEE2192.orf
With 182 enzymes: SgfI *
July 3, 2012 17:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1635 1722
Size: 1635 87
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 657 1085 1202 1424 1676 1722
Size: 367 290 428 117 222 252 46
Fragments arranged by size:
428 367 290 252 222 117 46
AflII C'TTAA_G
Cuts at: 0 406 1722
Size: 406 1316
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 744 776 1722
Size: 744 32 946
Fragments arranged by size:
946 744 32
AlwNI CAG_nnn'CTG
Cuts at: 0 817 1722
Size: 817 905
ApaLI G'TGCA_C
Cuts at: 0 1648 1722
Size: 1648 74
ApoI r'AATT_y
Cuts at: 0 963 1003 1619 1722
Size: 963 40 616 103
Fragments arranged by size:
963 616 103 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 307 1664 1722
Size: 307 1357 58
Fragments arranged by size:
1357 307 58
BbvCI CC'TCA_GC
Cuts at: 0 1445 1722
Size: 1445 277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 530 564 1722
Size: 530 34 1158
Fragments arranged by size:
1158 530 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1041 1445 1580 1722
Size: 1041 404 135 142
Fragments arranged by size:
1041 404 142 135
Bme1580I G_kGCm'C
Cuts at: 0 7 1652 1722
Size: 7 1645 70
Fragments arranged by size:
1645 70 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 20 1722
Size: 20 1702
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 899 1447 1488 1722
Size: 37 862 548 41 234
Fragments arranged by size:
862 548 234 41 37
Bpu10I CC'TnA_GC
Cuts at: 0 1445 1722
Size: 1445 277
BsaI GGTCTCn'nnnn_
Cuts at: 0 886 1507 1722
Size: 886 621 215
Fragments arranged by size:
886 621 215
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1292 1722
Size: 1292 430
BsiHKAI G_wGCw'C
Cuts at: 0 1652 1722
Size: 1652 70
BsmI GAATG_Cn'
Cuts at: 0 247 1722
Size: 247 1475
Bsp1286I G_dGCh'C
Cuts at: 0 7 1652 1722
Size: 7 1645 70
Fragments arranged by size:
1645 70 7
BspHI T'CATG_A
Cuts at: 0 1341 1680 1701 1722
Size: 1341 339 21 21
Fragments arranged by size:
1341 339 21 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1635 1722
Size: 1635 87
BstEII G'GTnAC_C
Cuts at: 0 1307 1722
Size: 1307 415
BstXI CCAn_nnnn'nTGG
Cuts at: 0 266 1722
Size: 266 1456
BstYI r'GATC_y
Cuts at: 0 355 1722
Size: 355 1367
Bsu36I CC'TnA_GG
Cuts at: 0 532 1389 1722
Size: 532 857 333
Fragments arranged by size:
857 532 333
BtgI C'CryG_G
Cuts at: 0 39 1348 1722
Size: 39 1309 374
Fragments arranged by size:
1309 374 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 189 1347 1722
Size: 189 1158 375
Fragments arranged by size:
1158 375 189
BtsI GCAGTG_nn'
Cuts at: 0 180 1567 1722
Size: 180 1387 155
Fragments arranged by size:
1387 180 155
DraI TTT'AAA
Cuts at: 0 1127 1722
Size: 1127 595
EarI CTCTTCn'nnn_
Cuts at: 0 748 1722
Size: 748 974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 367 657 899 1085 1202 1424 1447
Size: 37 330 290 242 186 117 222 23
Cuts at: 1447 1488 1676 1722
Size: 41 188 46
Fragments arranged by size:
330 290 242 222 188 186 117 46
41 37 23
EcoO109I rG'GnC_Cy
Cuts at: 0 536 1722
Size: 536 1186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1276 1351 1722
Size: 1276 75 371
Fragments arranged by size:
1276 371 75
HaeII r_GCGC'y
Cuts at: 0 75 1722
Size: 75 1647
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 12 44 480 512 1287 1319 1722
Size: 12 32 436 32 775 32 403
Fragments arranged by size:
775 436 403 32 32 32 12
HincII GTy'rAC
Cuts at: 0 669 1722
Size: 669 1053
HindIII A'AGCT_T
Cuts at: 0 1354 1722
Size: 1354 368
HpaI GTT'AAC
Cuts at: 0 669 1722
Size: 669 1053
MfeI C'AATT_G
Cuts at: 0 1574 1722
Size: 1574 148
MslI CAynn'nnrTG
Cuts at: 0 264 389 1722
Size: 264 125 1333
Fragments arranged by size:
1333 264 125
MspA1I CmG'CkG
Cuts at: 0 41 1444 1722
Size: 41 1403 278
Fragments arranged by size:
1403 278 41
NcoI C'CATG_G
Cuts at: 0 1348 1722
Size: 1348 374
NspI r_CATG'y
Cuts at: 0 887 1722
Size: 887 835
PpuMI rG'GwC_Cy
Cuts at: 0 536 1722
Size: 536 1186
PstI C_TGCA'G
Cuts at: 0 234 475 1722
Size: 234 241 1247
Fragments arranged by size:
1247 241 234
PvuII CAG'CTG
Cuts at: 0 1444 1722
Size: 1444 278
SacII CC_GC'GG
Cuts at: 0 42 1722
Size: 42 1680
SapI GCTCTTCn'nnn_
Cuts at: 0 748 1722
Size: 748 974
SfcI C'TryA_G
Cuts at: 0 230 471 1722
Size: 230 241 1251
Fragments arranged by size:
1251 241 230
SmlI C'TyrA_G
Cuts at: 0 406 1722
Size: 406 1316
StyI C'CwwG_G
Cuts at: 0 1348 1722
Size: 1348 374
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1295 1722
Size: 1295 427
TatI w'GTAC_w
Cuts at: 0 323 432 1722
Size: 323 109 1290
Fragments arranged by size:
1290 323 109
XmnI GAAnn'nnTTC
Cuts at: 0 1174 1722
Size: 1174 548
Enzymes that do cut:
AarI AcuI AflII AloI AlwNI ApaLI ApoI BbsI
BbvCI BcgI BciVI Bme1580I BmrI BpmI Bpu10I BsaI
BseRI BsiHKAI BsmI Bsp1286I BspHI BspMI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI EarI Eco57MI
EcoO109I FalI HaeII Hin4I HincII HindIII HpaI MfeI
MslI MspA1I NcoI NspI PpuMI PstI PvuII SacII
SapI SfcI SmlI StyI TaqII TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I ClaI CspCI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
KasI KpnI MluI MmeI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI