Restriction map for
pFN21AE2185
(Linear) (Six-base) MAPSORT of: KIEE2185.seq Check: 6034 from: 1 to: 846
KIEE2185.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 432 846
Size: 235 197 414
Fragments arranged by size:
414 235 197
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 271 303 846
Size: 271 32 543
Fragments arranged by size:
543 271 32
AlwNI CAG_nnn'CTG
Cuts at: 0 373 846
Size: 373 473
BanI G'GyrC_C
Cuts at: 0 376 846
Size: 376 470
BbvCI CC'TCA_GC
Cuts at: 0 129 846
Size: 129 717
BclI T'GATC_A
Cuts at: 0 315 846
Size: 315 531
Bme1580I G_kGCm'C
Cuts at: 0 813 846
Size: 813 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 621 846
Size: 621 225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 80 461 846
Size: 48 32 381 385
Fragments arranged by size:
385 381 48 32
Bpu10I CC'TnA_GC
Cuts at: 0 129 160 846
Size: 129 31 686
Fragments arranged by size:
686 129 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 846
Size: 448 398
BsaI GGTCTCn'nnnn_
Cuts at: 0 484 846
Size: 484 362
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 652 682 846
Size: 652 30 164
Fragments arranged by size:
652 164 30
BseYI C'CCAG_C
Cuts at: 0 133 524 806 846
Size: 133 391 282 40
Fragments arranged by size:
391 282 133 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 846
Size: 150 696
BsiHKAI G_wGCw'C
Cuts at: 0 641 846
Size: 641 205
BsmI GAATG_Cn'
Cuts at: 0 423 846
Size: 423 423
Bsp1286I G_dGCh'C
Cuts at: 0 641 813 846
Size: 641 172 33
Fragments arranged by size:
641 172 33
BsrDI GCAATG_nn'
Cuts at: 0 339 689 846
Size: 339 350 157
Fragments arranged by size:
350 339 157
BstAPI GCAn_nnn'nTGC
Cuts at: 0 310 846
Size: 310 536
BstYI r'GATC_y
Cuts at: 0 17 846
Size: 17 829
BtsI GCAGTG_nn'
Cuts at: 0 311 846
Size: 311 535
EarI CTCTTCn'nnn_
Cuts at: 0 28 792 846
Size: 28 764 54
Fragments arranged by size:
764 54 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 756 846
Size: 756 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 80 235 432 461 846
Size: 48 32 155 197 29 385
Fragments arranged by size:
385 197 155 48 32 29
EcoRV GAT'ATC
Cuts at: 0 198 711 846
Size: 198 513 135
Fragments arranged by size:
513 198 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 92 124 253 285 846
Size: 92 32 129 32 561
Fragments arranged by size:
561 129 92 32 32
HincII GTy'rAC
Cuts at: 0 555 846
Size: 555 291
MfeI C'AATT_G
Cuts at: 0 65 846
Size: 65 781
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 846
Size: 251 595
MspA1I CmG'CkG
Cuts at: 0 133 756 806 846
Size: 133 623 50 40
Fragments arranged by size:
623 133 50 40
NspI r_CATG'y
Cuts at: 0 456 846
Size: 456 390
PasI CC'CwG_GG
Cuts at: 0 9 846
Size: 9 837
PflMI CCAn_nnn'nTGG
Cuts at: 0 806 846
Size: 806 40
PvuII CAG'CTG
Cuts at: 0 133 756 806 846
Size: 133 623 50 40
Fragments arranged by size:
623 133 50 40
SfcI C'TryA_G
Cuts at: 0 751 846
Size: 751 95
SmlI C'TyrA_G
Cuts at: 0 427 846
Size: 427 419
SphI G_CATG'C
Cuts at: 0 456 846
Size: 456 390
Enzymes that do cut:
AcuI AloI AlwNI BanI BbvCI BclI Bme1580I BplI
BpmI Bpu10I BpuEI BsaI BsaXI BseYI BsgI BsiHKAI
BsmI Bsp1286I BsrDI BstAPI BstYI BtsI EarI EciI
Eco57MI EcoRV Hin4I HincII MfeI MmeI MspA1I NspI
PasI PflMI PvuII SfcI SmlI SphI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI
BbsI BcgI BciVI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MluI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI