Restriction map for
pFN21AE2180
(Linear) (Six-base) MAPSORT of: KIEE2180.seq Check: 9559 from: 1 to: 1251
KIEE2180.orf
With 182 enzymes: SgfI *
March 22, 2012 15:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 822 1251
Size: 822 429
AccI GT'mk_AC
Cuts at: 0 1010 1251
Size: 1010 241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 481 1251
Size: 351 130 770
Fragments arranged by size:
770 351 130
AfeI AGC'GCT
Cuts at: 0 62 310 390 667 1171 1251
Size: 62 248 80 277 504 80
Fragments arranged by size:
504 277 248 80 80 62
AlwNI CAG_nnn'CTG
Cuts at: 0 415 1244 1251
Size: 415 829 7
Fragments arranged by size:
829 415 7
ApaI G_GGCC'C
Cuts at: 0 940 1251
Size: 940 311
ApaLI G'TGCA_C
Cuts at: 0 424 1251
Size: 424 827
AvaI C'yCGr_G
Cuts at: 0 692 841 973 1251
Size: 692 149 132 278
Fragments arranged by size:
692 278 149 132
BanI G'GyrC_C
Cuts at: 0 948 1066 1251
Size: 948 118 185
Fragments arranged by size:
948 185 118
BanII G_rGCy'C
Cuts at: 0 158 940 1251
Size: 158 782 311
Fragments arranged by size:
782 311 158
BbvCI CC'TCA_GC
Cuts at: 0 249 1251
Size: 249 1002
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 252 286 1251
Size: 252 34 965
Fragments arranged by size:
965 252 34
Bme1580I G_kGCm'C
Cuts at: 0 428 450 940 951 1069 1251
Size: 428 22 490 11 118 182
Fragments arranged by size:
490 428 182 118 22 11
BmgBI CAC'GTC
Cuts at: 0 800 824 1251
Size: 800 24 427
Fragments arranged by size:
800 427 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 949 1251
Size: 349 600 302
Fragments arranged by size:
600 349 302
Bpu10I CC'TnA_GC
Cuts at: 0 199 249 562 1251
Size: 199 50 313 689
Fragments arranged by size:
689 313 199 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 802 1251
Size: 306 496 449
Fragments arranged by size:
496 449 306
BsaBI GATnn'nnATC
Cuts at: 0 274 1251
Size: 274 977
BsaHI Gr'CG_yC
Cuts at: 0 838 1251
Size: 838 413
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 514 1251
Size: 514 737
BseYI C'CCAG_C
Cuts at: 0 41 47 585 939 1251
Size: 41 6 538 354 312
Fragments arranged by size:
538 354 312 41 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 1251
Size: 505 746
BsiEI CG_ry'CG
Cuts at: 0 904 1134 1251
Size: 904 230 117
Fragments arranged by size:
904 230 117
BsiHKAI G_wGCw'C
Cuts at: 0 74 164 428 1251
Size: 74 90 264 823
Fragments arranged by size:
823 264 90 74
BsmI GAATG_Cn'
Cuts at: 0 289 1251
Size: 289 962
Bsp1286I G_dGCh'C
Cuts at: 0 74 158 164 428 450 940 951 1069
Size: 74 84 6 264 22 490 11 118
Cuts at: 1069 1251
Size: 182
Fragments arranged by size:
490 264 182 118 84 74 22 11
6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 822 1251
Size: 822 429
BsrDI GCAATG_nn'
Cuts at: 0 187 1251
Size: 187 1064
BsrFI r'CCGG_y
Cuts at: 0 203 957 1099 1251
Size: 203 754 142 152
Fragments arranged by size:
754 203 152 142
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1124 1127 1251
Size: 1124 3 124
Fragments arranged by size:
1124 124 3
BstBI TT'CG_AA
Cuts at: 0 1016 1251
Size: 1016 235
BstXI CCAn_nnnn'nTGG
Cuts at: 0 65 1251
Size: 65 1186
BstYI r'GATC_y
Cuts at: 0 344 1251
Size: 344 907
BtgI C'CryG_G
Cuts at: 0 52 567 792 854 1070 1134 1251
Size: 52 515 225 62 216 64 117
Fragments arranged by size:
515 225 216 117 64 62 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 390 1251
Size: 390 861
BtsI GCAGTG_nn'
Cuts at: 0 541 764 1176 1251
Size: 541 223 412 75
Fragments arranged by size:
541 412 223 75
DraIII CAC_nnn'GTG
Cuts at: 0 179 432 1232 1251
Size: 179 253 800 19
Fragments arranged by size:
800 253 179 19
EaeI y'GGCC_r
Cuts at: 0 55 901 921 1131 1137 1251
Size: 55 846 20 210 6 114
Fragments arranged by size:
846 210 114 55 20 6
EagI C'GGCC_G
Cuts at: 0 901 1131 1251
Size: 901 230 120
Fragments arranged by size:
901 230 120
EarI CTCTTCn'nnn_
Cuts at: 0 1208 1251
Size: 1208 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 130 792 1251
Size: 130 662 459
Fragments arranged by size:
662 459 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 351 481 949 1251
Size: 349 2 130 468 302
Fragments arranged by size:
468 349 302 130 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 510 1251
Size: 510 741
EcoO109I rG'GnC_Cy
Cuts at: 0 558 573 1251
Size: 558 15 678
Fragments arranged by size:
678 558 15
FspI TGC'GCA
Cuts at: 0 871 1251
Size: 871 380
HaeII r_GCGC'y
Cuts at: 0 11 64 312 392 669 865 971 1100
Size: 11 53 248 80 277 196 106 129
Cuts at: 1100 1173 1251
Size: 73 78
Fragments arranged by size:
277 248 196 129 106 80 78 73
53 11
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 1251
Size: 60 1191
MslI CAynn'nnrTG
Cuts at: 0 236 1251
Size: 236 1015
MspA1I CmG'CkG
Cuts at: 0 54 415 809 927 943 1136 1251
Size: 54 361 394 118 16 193 115
Fragments arranged by size:
394 361 193 118 115 54 16
NaeI GCC'GGC
Cuts at: 0 205 959 1101 1251
Size: 205 754 142 150
Fragments arranged by size:
754 205 150 142
NcoI C'CATG_G
Cuts at: 0 567 1251
Size: 567 684
NgoMIV G'CCGG_C
Cuts at: 0 203 957 1099 1251
Size: 203 754 142 152
Fragments arranged by size:
754 203 152 142
NotI GC'GGCC_GC
Cuts at: 0 1131 1251
Size: 1131 120
NspI r_CATG'y
Cuts at: 0 774 1125 1251
Size: 774 351 126
Fragments arranged by size:
774 351 126
PasI CC'CwG_GG
Cuts at: 0 450 1251
Size: 450 801
PfoI T'CCnGG_A
Cuts at: 0 575 1251
Size: 575 676
PpuMI rG'GwC_Cy
Cuts at: 0 573 1251
Size: 573 678
PspOMI G'GGCC_C
Cuts at: 0 936 1251
Size: 936 315
PstI C_TGCA'G
Cuts at: 0 548 617 643 1251
Size: 548 69 26 608
Fragments arranged by size:
608 548 69 26
PvuII CAG'CTG
Cuts at: 0 415 809 927 1251
Size: 415 394 118 324
Fragments arranged by size:
415 394 324 118
SacII CC_GC'GG
Cuts at: 0 55 1137 1251
Size: 55 1082 114
Fragments arranged by size:
1082 114 55
SanDI GG'GwC_CC
Cuts at: 0 573 1251
Size: 573 678
SbfI CC_TGCA'GG
Cuts at: 0 617 1251
Size: 617 634
SexAI A'CCwGG_T
Cuts at: 0 1004 1251
Size: 1004 247
SfcI C'TryA_G
Cuts at: 0 544 613 639 1251
Size: 544 69 26 612
Fragments arranged by size:
612 544 69 26
SmaI CCC'GGG
Cuts at: 0 694 975 1251
Size: 694 281 276
Fragments arranged by size:
694 281 276
SmlI C'TyrA_G
Cuts at: 0 321 817 1251
Size: 321 496 434
Fragments arranged by size:
496 434 321
StuI AGG'CCT
Cuts at: 0 197 466 505 611 1251
Size: 197 269 39 106 640
Fragments arranged by size:
640 269 197 106 39
StyI C'CwwG_G
Cuts at: 0 567 1251
Size: 567 684
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 57 135 599 1251
Size: 57 78 464 652
Fragments arranged by size:
652 464 78 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 418 450 1251
Size: 418 32 801
Fragments arranged by size:
801 418 32
Tth111I GACn'n_nGTC
Cuts at: 0 1006 1251
Size: 1006 245
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 129 1251
Size: 129 1122
XmaI C'CCGG_G
Cuts at: 0 692 973 1251
Size: 692 281 278
Fragments arranged by size:
692 281 278
Enzymes that do cut:
AarI AccI AcuI AfeI AlwNI ApaI ApaLI AvaI
BanI BanII BbvCI BcgI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaBI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BsrFI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I FspI HaeII MmeI MslI
MspA1I NaeI NcoI NgoMIV NotI NspI PasI PfoI
PpuMI PspOMI PstI PvuII SacII SanDI SbfI SexAI
SfcI SmaI SmlI StuI StyI TaqII TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI BmrI BmtI BplI BsaI BsaAI BsaWI BsaXI
BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoICRI
EcoRI EcoRV FalI FseI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NarI NdeI
NheI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SalI SapI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TatI XbaI XhoI
XmnI ZraI