Restriction map for
pFN21AE2173
(Linear) (Six-base) MAPSORT of: KIEE2173.seq Check: 6645 from: 1 to: 2193
KIEE2173.orf
With 182 enzymes: SgfI *
March 22, 2012 15:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 1033 1102 1633 2193
Size: 893 140 69 531 560
Fragments arranged by size:
893 560 531 140 69
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1969 2193
Size: 1969 224
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1249 1281 2193
Size: 1249 32 912
Fragments arranged by size:
1249 912 32
AlwNI CAG_nnn'CTG
Cuts at: 0 199 352 699 942 1452 1548 2193
Size: 199 153 347 243 510 96 645
Fragments arranged by size:
645 510 347 243 199 153 96
ApoI r'AATT_y
Cuts at: 0 519 1161 1697 2063 2193
Size: 519 642 536 366 130
Fragments arranged by size:
642 536 519 366 130
AseI AT'TA_AT
Cuts at: 0 1282 1916 2193
Size: 1282 634 277
Fragments arranged by size:
1282 634 277
AvaI C'yCGr_G
Cuts at: 0 839 2193
Size: 839 1354
AvrII C'CTAG_G
Cuts at: 0 310 2193
Size: 310 1883
BanII G_rGCy'C
Cuts at: 0 457 855 2193
Size: 457 398 1338
Fragments arranged by size:
1338 457 398
BbsI GAAGACnn'nnnn_
Cuts at: 0 1901 2193
Size: 1901 292
BbvCI CC'TCA_GC
Cuts at: 0 700 2193
Size: 700 1493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1108 1142 2193
Size: 1108 34 1051
Fragments arranged by size:
1108 1051 34
BclI T'GATC_A
Cuts at: 0 54 2040 2193
Size: 54 1986 153
Fragments arranged by size:
1986 153 54
BfrBI ATG'CAT
Cuts at: 0 301 2193
Size: 301 1892
BglII A'GATC_T
Cuts at: 0 36 1641 2193
Size: 36 1605 552
Fragments arranged by size:
1605 552 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 482 2193
Size: 482 1711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 907 1624 1834 1876 2193
Size: 706 201 717 210 42 317
Fragments arranged by size:
717 706 317 210 201 42
Bpu10I CC'TnA_GC
Cuts at: 0 700 2193
Size: 700 1493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 2193
Size: 749 1444
BsaAI yAC'GTr
Cuts at: 0 182 2193
Size: 182 2011
BsaBI GATnn'nnATC
Cuts at: 0 1646 2193
Size: 1646 547
BseYI C'CCAG_C
Cuts at: 0 896 1564 2193
Size: 896 668 629
Fragments arranged by size:
896 668 629
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1112 2193
Size: 1112 1081
BsiHKAI G_wGCw'C
Cuts at: 0 457 2193
Size: 457 1736
BsmI GAATG_Cn'
Cuts at: 0 813 2193
Size: 813 1380
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1682 2193
Size: 1682 511
Bsp1286I G_dGCh'C
Cuts at: 0 457 855 2193
Size: 457 398 1338
Fragments arranged by size:
1338 457 398
BspHI T'CATG_A
Cuts at: 0 2135 2193
Size: 2135 58
BsrDI GCAATG_nn'
Cuts at: 0 1663 2193
Size: 1663 530
BstBI TT'CG_AA
Cuts at: 0 210 2193
Size: 210 1983
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1395 1564 2185 2193
Size: 1395 169 621 8
Fragments arranged by size:
1395 621 169 8
BstYI r'GATC_y
Cuts at: 0 36 259 751 1477 1545 1641 2193
Size: 36 223 492 726 68 96 552
Fragments arranged by size:
726 552 492 223 96 68 36
BtsI GCAGTG_nn'
Cuts at: 0 239 1382 2193
Size: 239 1143 811
Fragments arranged by size:
1143 811 239
DraI TTT'AAA
Cuts at: 0 44 722 2193
Size: 44 678 1471
Fragments arranged by size:
1471 678 44
EaeI y'GGCC_r
Cuts at: 0 1184 2193
Size: 1184 1009
EarI CTCTTCn'nnn_
Cuts at: 0 1052 1078 1207 2193
Size: 1052 26 129 986
Fragments arranged by size:
1052 986 129 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1872 2193
Size: 1872 321
EcoICRI GAG'CTC
Cuts at: 0 455 2193
Size: 455 1738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 893 907 1033 1102 1624 1633 1834
Size: 706 187 14 126 69 522 9 201
Cuts at: 1834 1876 2193
Size: 42 317
Fragments arranged by size:
706 522 317 201 187 126 69 42
14 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1275 1660 2193
Size: 1275 385 533
Fragments arranged by size:
1275 533 385
HaeII r_GCGC'y
Cuts at: 0 1863 2193
Size: 1863 330
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 643 675 2193
Size: 643 32 1518
Fragments arranged by size:
1518 643 32
HincII GTy'rAC
Cuts at: 0 2150 2193
Size: 2150 43
MfeI C'AATT_G
Cuts at: 0 2036 2193
Size: 2036 157
MspA1I CmG'CkG
Cuts at: 0 199 696 900 2193
Size: 199 497 204 1293
Fragments arranged by size:
1293 497 204 199
NdeI CA'TA_TG
Cuts at: 0 2184 2193
Size: 2184 9
NsiI A_TGCA'T
Cuts at: 0 303 2193
Size: 303 1890
PfoI T'CCnGG_A
Cuts at: 0 923 2193
Size: 923 1270
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1870 1902 2193
Size: 1870 32 291
Fragments arranged by size:
1870 291 32
PshAI GACnn'nnGTC
Cuts at: 0 442 2193
Size: 442 1751
PspXI vC'TCGA_Gb
Cuts at: 0 839 2193
Size: 839 1354
PstI C_TGCA'G
Cuts at: 0 1028 1043 1232 2193
Size: 1028 15 189 961
Fragments arranged by size:
1028 961 189 15
PvuII CAG'CTG
Cuts at: 0 199 696 900 2193
Size: 199 497 204 1293
Fragments arranged by size:
1293 497 204 199
SacI G_AGCT'C
Cuts at: 0 457 2193
Size: 457 1736
ScaI AGT'ACT
Cuts at: 0 948 2193
Size: 948 1245
SfcI C'TryA_G
Cuts at: 0 1024 1039 1228 2193
Size: 1024 15 189 965
Fragments arranged by size:
1024 965 189 15
SmlI C'TyrA_G
Cuts at: 0 764 839 2193
Size: 764 75 1354
Fragments arranged by size:
1354 764 75
SnaBI TAC'GTA
Cuts at: 0 182 2193
Size: 182 2011
StuI AGG'CCT
Cuts at: 0 280 315 1370 2193
Size: 280 35 1055 823
Fragments arranged by size:
1055 823 280 35
StyI C'CwwG_G
Cuts at: 0 310 2193
Size: 310 1883
TatI w'GTAC_w
Cuts at: 0 906 946 2193
Size: 906 40 1247
Fragments arranged by size:
1247 906 40
XhoI C'TCGA_G
Cuts at: 0 839 2193
Size: 839 1354
XmnI GAAnn'nnTTC
Cuts at: 0 1409 1697 2193
Size: 1409 288 496
Fragments arranged by size:
1409 496 288
Enzymes that do cut:
AcuI AlfI AloI AlwNI ApoI AseI AvaI AvrII
BanII BbsI BbvCI BcgI BclI BfrBI BglII BmrI
BpmI Bpu10I BpuEI BsaAI BsaBI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BsrDI BstBI BstXI BstYI
BtsI DraI EaeI EarI EciI EcoICRI Eco57MI FalI
HaeII Hin4I HincII MfeI MspA1I NdeI NsiI PfoI
PpiI PshAI PspXI PstI PvuII SacI ScaI SfcI
SmlI SnaBI StuI StyI TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI ApaI ApaLI AscI AsiSI BaeI
BamHI BanI BbeI BciVI BglI BlpI Bme1580I BmgBI
BmtI BplI BsaI BsaHI BsaWI BsaXI BseRI BsiEI
BsiWI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraIII DrdI EagI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KasI KpnI MluI MmeI
MscI MslI NaeI NarI NcoI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PflMI PmeI PmlI
PpuMI PsiI PspOMI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SpeI SphI SrfI SspI SwaI TaqII TstI
Tth111I XbaI XcmI XmaI ZraI