Restriction map for
pFN21AE2164
(Linear) (Six-base) MAPSORT of: KIEE2164.seq Check: 1158 from: 1 to: 876
KIEE2164.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AflIII A'CryG_T
Cuts at: 0 308 876
Size: 308 568
AlwNI CAG_nnn'CTG
Cuts at: 0 100 876
Size: 100 776
ApoI r'AATT_y
Cuts at: 0 261 801 876
Size: 261 540 75
Fragments arranged by size:
540 261 75
BanII G_rGCy'C
Cuts at: 0 426 791 876
Size: 426 365 85
Fragments arranged by size:
426 365 85
BbsI GAAGACnn'nnnn_
Cuts at: 0 607 635 876
Size: 607 28 241
Fragments arranged by size:
607 241 28
BglII A'GATC_T
Cuts at: 0 756 876
Size: 756 120
Bme1580I G_kGCm'C
Cuts at: 0 439 876
Size: 439 437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 142 876
Size: 77 65 734
Fragments arranged by size:
734 77 65
Bpu10I CC'TnA_GC
Cuts at: 0 136 698 876
Size: 136 562 178
Fragments arranged by size:
562 178 136
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 226 229 376 594 876
Size: 193 33 3 147 218 282
Fragments arranged by size:
282 218 193 147 33 3
BseYI C'CCAG_C
Cuts at: 0 556 876
Size: 556 320
BsiHKAI G_wGCw'C
Cuts at: 0 349 876
Size: 349 527
BsmI GAATG_Cn'
Cuts at: 0 117 876
Size: 117 759
Bsp1286I G_dGCh'C
Cuts at: 0 349 426 439 791 876
Size: 349 77 13 352 85
Fragments arranged by size:
352 349 85 77 13
BspHI T'CATG_A
Cuts at: 0 189 876
Size: 189 687
BsrBI CCG'CTC
Cuts at: 0 226 876
Size: 226 650
BsrFI r'CCGG_y
Cuts at: 0 829 876
Size: 829 47
BstYI r'GATC_y
Cuts at: 0 36 654 756 876
Size: 36 618 102 120
Fragments arranged by size:
618 120 102 36
BtgI C'CryG_G
Cuts at: 0 794 876
Size: 794 82
BtsI GCAGTG_nn'
Cuts at: 0 204 876
Size: 204 672
EaeI y'GGCC_r
Cuts at: 0 518 876
Size: 518 358
EarI CTCTTCn'nnn_
Cuts at: 0 170 314 330 335 728 876
Size: 170 144 16 5 393 148
Fragments arranged by size:
393 170 148 144 16 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 142 876
Size: 77 65 734
Fragments arranged by size:
734 77 65
EcoRI G'AATT_C
Cuts at: 0 261 876
Size: 261 615
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 698 876
Size: 698 178
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 622 654 876
Size: 229 32 361 32 222
Fragments arranged by size:
361 229 222 32 32
HindIII A'AGCT_T
Cuts at: 0 680 876
Size: 680 196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 801 876
Size: 293 508 75
Fragments arranged by size:
508 293 75
MscI TGG'CCA
Cuts at: 0 520 876
Size: 520 356
NspI r_CATG'y
Cuts at: 0 312 876
Size: 312 564
PciI A'CATG_T
Cuts at: 0 308 876
Size: 308 568
PfoI T'CCnGG_A
Cuts at: 0 158 876
Size: 158 718
PstI C_TGCA'G
Cuts at: 0 199 489 535 876
Size: 199 290 46 341
Fragments arranged by size:
341 290 199 46
SfcI C'TryA_G
Cuts at: 0 195 485 531 876
Size: 195 290 46 345
Fragments arranged by size:
345 290 195 46
SspI AAT'ATT
Cuts at: 0 575 876
Size: 575 301
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 64 96 876
Size: 64 32 780
Fragments arranged by size:
780 64 32
Enzymes that do cut:
AflIII AlwNI ApoI BanII BbsI BglII Bme1580I BpmI
Bpu10I BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BsrBI
BsrFI BstYI BtgI BtsI EaeI EarI Eco57MI EcoRI
FalI Hin4I HindIII MmeI MscI NspI PciI PfoI
PstI SfcI SspI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmgBI
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspMI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII
TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI