Restriction map for
pFN21AE2162
(Linear) (Six-base) MAPSORT of: KIEE2162.seq Check: 6156 from: 1 to: 849
KIEE2162.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 849
Size: 304 545
AhdI GACnn_n'nnGTC
Cuts at: 0 167 849
Size: 167 682
AleI CACnn'nnGTG
Cuts at: 0 157 547 849
Size: 157 390 302
Fragments arranged by size:
390 302 157
ApoI r'AATT_y
Cuts at: 0 118 465 529 838 849
Size: 118 347 64 309 11
Fragments arranged by size:
347 309 118 64 11
AvaI C'yCGr_G
Cuts at: 0 485 849
Size: 485 364
BanI G'GyrC_C
Cuts at: 0 244 849
Size: 244 605
BbsI GAAGACnn'nnnn_
Cuts at: 0 42 849
Size: 42 807
BciVI GTATCCnnnnn_n'
Cuts at: 0 730 849
Size: 730 119
BlpI GC'TnA_GC
Cuts at: 0 135 849
Size: 135 714
BmrI ACTGGGnnnn_n'
Cuts at: 0 329 849
Size: 329 520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 849
Size: 702 147
Bpu10I CC'TnA_GC
Cuts at: 0 129 849
Size: 129 720
BsaI GGTCTCn'nnnn_
Cuts at: 0 142 601 849
Size: 142 459 248
Fragments arranged by size:
459 248 142
BsaAI yAC'GTr
Cuts at: 0 17 277 849
Size: 17 260 572
Fragments arranged by size:
572 260 17
BsaHI Gr'CG_yC
Cuts at: 0 689 849
Size: 689 160
BseYI C'CCAG_C
Cuts at: 0 400 849
Size: 400 449
BsiHKAI G_wGCw'C
Cuts at: 0 365 423 849
Size: 365 58 426
Fragments arranged by size:
426 365 58
Bsp1286I G_dGCh'C
Cuts at: 0 365 423 849
Size: 365 58 426
Fragments arranged by size:
426 365 58
BsrDI GCAATG_nn'
Cuts at: 0 395 849
Size: 395 454
BsrFI r'CCGG_y
Cuts at: 0 451 849
Size: 451 398
BstXI CCAn_nnnn'nTGG
Cuts at: 0 823 849
Size: 823 26
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 792 827 849
Size: 792 35 22
Fragments arranged by size:
792 35 22
EaeI y'GGCC_r
Cuts at: 0 449 813 849
Size: 449 364 36
Fragments arranged by size:
449 364 36
EarI CTCTTCn'nnn_
Cuts at: 0 788 849
Size: 788 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 702 849
Size: 304 398 147
Fragments arranged by size:
398 304 147
EcoRI G'AATT_C
Cuts at: 0 529 849
Size: 529 320
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 286 309 849
Size: 286 23 540
Fragments arranged by size:
540 286 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 153 185 849
Size: 153 32 664
Fragments arranged by size:
664 153 32
HindIII A'AGCT_T
Cuts at: 0 82 820 849
Size: 82 738 29
Fragments arranged by size:
738 82 29
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 849
Size: 584 265
MscI TGG'CCA
Cuts at: 0 815 849
Size: 815 34
MslI CAynn'nnrTG
Cuts at: 0 157 545 547 849
Size: 157 388 2 302
Fragments arranged by size:
388 302 157 2
MspA1I CmG'CkG
Cuts at: 0 613 849
Size: 613 236
NaeI GCC'GGC
Cuts at: 0 453 849
Size: 453 396
NgoMIV G'CCGG_C
Cuts at: 0 451 849
Size: 451 398
PmlI CAC'GTG
Cuts at: 0 277 849
Size: 277 572
PshAI GACnn'nnGTC
Cuts at: 0 613 849
Size: 613 236
SapI GCTCTTCn'nnn_
Cuts at: 0 788 849
Size: 788 61
StyI C'CwwG_G
Cuts at: 0 56 849
Size: 56 793
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 27 849
Size: 27 822
TatI w'GTAC_w
Cuts at: 0 182 849
Size: 182 667
Tth111I GACn'n_nGTC
Cuts at: 0 774 849
Size: 774 75
Enzymes that do cut:
AcuI AhdI AleI ApoI AvaI BanI BbsI BciVI
BlpI BmrI BpmI Bpu10I BsaI BsaAI BsaHI BseYI
BsiHKAI Bsp1286I BsrDI BsrFI BstXI CspCI EaeI EarI
Eco57MI EcoRI FalI Hin4I HindIII MmeI MscI MslI
MspA1I NaeI NgoMIV PmlI PshAI SapI StyI TaqII
TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BclI BfrBI BglI BglII Bme1580I BmgBI BmtI BplI
BpuEI BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI XbaI XcmI XhoI XmaI
XmnI ZraI