(Linear) (Six-base) MAPSORT of: KIEE2161.seq  Check: 3012  from: 1  to: 363



KIEE2161.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:55  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      21     363 
   Size:         21     342
 
AvaI C'yCGr_G

Cuts at:      0     229     351     363 
   Size:        229     122      12

  Fragments arranged by size: 

                 229     122      12
 
BamHI G'GATC_C

Cuts at:      0     117     363 
   Size:        117     246
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     356     363 
   Size:        356       7
 
BfrBI ATG'CAT

Cuts at:      0     246     363 
   Size:        246     117
 
BglI GCCn_nnn'nGGC

Cuts at:      0      31     363 
   Size:         31     332
 
BglII A'GATC_T

Cuts at:      0     123     363 
   Size:        123     240
 
BmtI G_CTAG'C

Cuts at:      0     203     363 
   Size:        203     160
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     190     363 
   Size:        124      66     173

  Fragments arranged by size: 

                 173     124      66
 
Bpu10I CC'TnA_GC

Cuts at:      0     190     363 
   Size:        190     173
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325     363 
   Size:        325      38
 
BsaBI GATnn'nnATC

Cuts at:      0     122     308     363 
   Size:        122     186      55

  Fragments arranged by size: 

                 186     122      55
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      97     291     363 
   Size:         97     194      72

  Fragments arranged by size: 

                 194      97      72
 
BseYI C'CCAG_C

Cuts at:      0     325     363 
   Size:        325      38
 
BstYI r'GATC_y

Cuts at:      0     117     123     207     363 
   Size:        117       6      84     156

  Fragments arranged by size: 

                 156     117      84       6
 
Bsu36I CC'TnA_GG

Cuts at:      0     212     363 
   Size:        212     151
 
EarI CTCTTCn'nnn_

Cuts at:      0     154     363 
   Size:        154     209
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      21     124     190     363 
   Size:         21     103      66     173

  Fragments arranged by size: 

                 173     103      66      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     193     363 
   Size:        193     170
 
MspA1I CmG'CkG

Cuts at:      0     135     363 
   Size:        135     228
 
NheI G'CTAG_C

Cuts at:      0     199     363 
   Size:        199     164
 
NsiI A_TGCA'T

Cuts at:      0     248     363 
   Size:        248     115
 
NspI r_CATG'y

Cuts at:      0     246     363 
   Size:        246     117
 
PvuII CAG'CTG

Cuts at:      0     135     363 
   Size:        135     228
 
SmlI C'TyrA_G

Cuts at:      0     340     363 
   Size:        340      23
 

 Enzymes that do cut:

     AcuI     AvaI    BamHI    BciVI    BfrBI     BglI    BglII     BmtI
     BpmI   Bpu10I    BpuEI    BsaBI    BseRI    BseYI    BstYI   Bsu36I
     EarI  Eco57MI    EcoNI   MspA1I     NheI     NsiI     NspI    PvuII
     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI     BlpI Bme1580I    BmgBI
     BmrI     BplI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI