Restriction map for
pFN21AE2157
(Linear) (Six-base) MAPSORT of: KIEE2157.seq Check: 5649 from: 1 to: 990
KIEE2157.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 990
Size: 281 709
AflIII A'CryG_T
Cuts at: 0 748 946 990
Size: 748 198 44
Fragments arranged by size:
748 198 44
AleI CACnn'nnGTG
Cuts at: 0 243 990
Size: 243 747
ApaI G_GGCC'C
Cuts at: 0 629 990
Size: 629 361
ApoI r'AATT_y
Cuts at: 0 92 889 990
Size: 92 797 101
Fragments arranged by size:
797 101 92
AvaI C'yCGr_G
Cuts at: 0 806 990
Size: 806 184
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 952 985 990
Size: 952 33 5
Fragments arranged by size:
952 33 5
BanII G_rGCy'C
Cuts at: 0 535 629 990
Size: 535 94 361
Fragments arranged by size:
535 361 94
BciVI GTATCCnnnnn_n'
Cuts at: 0 343 980 990
Size: 343 637 10
Fragments arranged by size:
637 343 10
Bme1580I G_kGCm'C
Cuts at: 0 629 990
Size: 629 361
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 985 990
Size: 668 317 5
Fragments arranged by size:
668 317 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 990
Size: 748 242
Bsp1286I G_dGCh'C
Cuts at: 0 535 629 990
Size: 535 94 361
Fragments arranged by size:
535 361 94
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 410 990
Size: 410 580
BsrDI GCAATG_nn'
Cuts at: 0 934 990
Size: 934 56
BstAPI GCAn_nnn'nTGC
Cuts at: 0 212 990
Size: 212 778
BstEII G'GTnAC_C
Cuts at: 0 230 990
Size: 230 760
BtgI C'CryG_G
Cuts at: 0 87 990
Size: 87 903
DraI TTT'AAA
Cuts at: 0 572 990
Size: 572 418
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 437 990
Size: 437 553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 668 985 990
Size: 281 387 317 5
Fragments arranged by size:
387 317 281 5
EcoO109I rG'GnC_Cy
Cuts at: 0 626 990
Size: 626 364
EcoRI G'AATT_C
Cuts at: 0 92 990
Size: 92 898
EcoRV GAT'ATC
Cuts at: 0 38 910 990
Size: 38 872 80
Fragments arranged by size:
872 80 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 480 512 990
Size: 480 32 478
Fragments arranged by size:
480 478 32
HindIII A'AGCT_T
Cuts at: 0 859 990
Size: 859 131
MslI CAynn'nnrTG
Cuts at: 0 243 597 780 794 840 990
Size: 243 354 183 14 46 150
Fragments arranged by size:
354 243 183 150 46 14
MspA1I CmG'CkG
Cuts at: 0 886 990
Size: 886 104
NcoI C'CATG_G
Cuts at: 0 87 990
Size: 87 903
NspI r_CATG'y
Cuts at: 0 215 752 950 990
Size: 215 537 198 40
Fragments arranged by size:
537 215 198 40
PciI A'CATG_T
Cuts at: 0 748 946 990
Size: 748 198 44
Fragments arranged by size:
748 198 44
PshAI GACnn'nnGTC
Cuts at: 0 765 990
Size: 765 225
PsiI TTA'TAA
Cuts at: 0 827 990
Size: 827 163
PspOMI G'GGCC_C
Cuts at: 0 625 990
Size: 625 365
PvuII CAG'CTG
Cuts at: 0 886 990
Size: 886 104
SfcI C'TryA_G
Cuts at: 0 137 990
Size: 137 853
SmlI C'TyrA_G
Cuts at: 0 727 990
Size: 727 263
StyI C'CwwG_G
Cuts at: 0 87 990
Size: 87 903
TatI w'GTAC_w
Cuts at: 0 456 990
Size: 456 534
Enzymes that do cut:
AcuI AflIII AleI ApaI ApoI AvaI BaeI BanII
BciVI Bme1580I BpmI BpuEI Bsp1286I BspMI BsrDI BstAPI
BstEII BtgI DraI EciI Eco57MI EcoO109I EcoRI EcoRV
Hin4I HindIII MslI MspA1I NcoI NspI PciI PshAI
PsiI PspOMI PvuII SfcI SmlI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BamHI BanI BbeI BbsI BbvCI BcgI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstXI
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII
DrdI EaeI EagI EarI EcoICRI EcoNI FalI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PpuMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI