Restriction map for
pFN21AE2156
(Linear) (Six-base) MAPSORT of: KIEE2156.seq Check: 7665 from: 1 to: 1257
KIEE2156.orf
With 182 enzymes: SgfI *
July 11, 2011 16:50 ..
AccI GT'mk_AC
Cuts at: 0 163 1257
Size: 163 1094
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 517 1257
Size: 143 374 740
Fragments arranged by size:
740 374 143
AflIII A'CryG_T
Cuts at: 0 499 708 1257
Size: 499 209 549
Fragments arranged by size:
549 499 209
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 138 170 1257
Size: 138 32 1087
Fragments arranged by size:
1087 138 32
AlwNI CAG_nnn'CTG
Cuts at: 0 723 1257
Size: 723 534
ApoI r'AATT_y
Cuts at: 0 25 796 998 1257
Size: 25 771 202 259
Fragments arranged by size:
771 259 202 25
AseI AT'TA_AT
Cuts at: 0 913 1257
Size: 913 344
AvaI C'yCGr_G
Cuts at: 0 902 1257
Size: 902 355
BbsI GAAGACnn'nnnn_
Cuts at: 0 1077 1257
Size: 1077 180
BfrBI ATG'CAT
Cuts at: 0 274 1257
Size: 274 983
BglII A'GATC_T
Cuts at: 0 126 1104 1257
Size: 126 978 153
Fragments arranged by size:
978 153 126
BmgBI CAC'GTC
Cuts at: 0 579 1257
Size: 579 678
BmtI G_CTAG'C
Cuts at: 0 569 1257
Size: 569 688
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 658 1257
Size: 658 599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1235 1257
Size: 1235 22
BsaAI yAC'GTr
Cuts at: 0 709 1257
Size: 709 548
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 473 1257
Size: 473 784
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 1257
Size: 68 1189
BsiEI CG_ry'CG
Cuts at: 0 653 1257
Size: 653 604
BsiHKAI G_wGCw'C
Cuts at: 0 762 1257
Size: 762 495
Bsp1286I G_dGCh'C
Cuts at: 0 762 1257
Size: 762 495
BspHI T'CATG_A
Cuts at: 0 522 1257
Size: 522 735
BsrFI r'CCGG_y
Cuts at: 0 1226 1257
Size: 1226 31
BstYI r'GATC_y
Cuts at: 0 126 218 828 1104 1257
Size: 126 92 610 276 153
Fragments arranged by size:
610 276 153 126 92
BstZ17I GTA'TAC
Cuts at: 0 164 1257
Size: 164 1093
BtgI C'CryG_G
Cuts at: 0 753 1257
Size: 753 504
BtsI GCAGTG_nn'
Cuts at: 0 56 1257
Size: 56 1201
EaeI y'GGCC_r
Cuts at: 0 703 1257
Size: 703 554
EarI CTCTTCn'nnn_
Cuts at: 0 1190 1257
Size: 1190 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 517 1257
Size: 143 374 740
Fragments arranged by size:
740 374 143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 717 749 1110 1142 1257
Size: 717 32 361 32 115
Fragments arranged by size:
717 361 115 32 32
HincII GTy'rAC
Cuts at: 0 251 1257
Size: 251 1006
HindIII A'AGCT_T
Cuts at: 0 874 1257
Size: 874 383
MfeI C'AATT_G
Cuts at: 0 95 1257
Size: 95 1162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1257
Size: 475 782
MspA1I CmG'CkG
Cuts at: 0 823 1191 1257
Size: 823 368 66
Fragments arranged by size:
823 368 66
NcoI C'CATG_G
Cuts at: 0 753 1257
Size: 753 504
NheI G'CTAG_C
Cuts at: 0 565 1257
Size: 565 692
NsiI A_TGCA'T
Cuts at: 0 276 1257
Size: 276 981
NspI r_CATG'y
Cuts at: 0 503 1179 1257
Size: 503 676 78
Fragments arranged by size:
676 503 78
PciI A'CATG_T
Cuts at: 0 499 1257
Size: 499 758
PmlI CAC'GTG
Cuts at: 0 709 1257
Size: 709 548
PvuII CAG'CTG
Cuts at: 0 1191 1257
Size: 1191 66
SfcI C'TryA_G
Cuts at: 0 803 1050 1257
Size: 803 247 207
Fragments arranged by size:
803 247 207
SmaI CCC'GGG
Cuts at: 0 904 1257
Size: 904 353
SmlI C'TyrA_G
Cuts at: 0 1250 1257
Size: 1250 7
SpeI A'CTAG_T
Cuts at: 0 1212 1257
Size: 1212 45
SphI G_CATG'C
Cuts at: 0 1179 1257
Size: 1179 78
StyI C'CwwG_G
Cuts at: 0 753 1257
Size: 753 504
TatI w'GTAC_w
Cuts at: 0 71 1257
Size: 71 1186
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 783 815 1257
Size: 783 32 442
Fragments arranged by size:
783 442 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 175 1257
Size: 175 1082
XmaI C'CCGG_G
Cuts at: 0 902 1257
Size: 902 355
XmnI GAAnn'nnTTC
Cuts at: 0 373 530 776 1001 1257
Size: 373 157 246 225 256
Fragments arranged by size:
373 256 246 225 157
Enzymes that do cut:
AccI AcuI AflIII AloI AlwNI ApoI AseI AvaI
BbsI BfrBI BglII BmgBI BmtI BplI BpuEI BsaAI
BseRI BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrFI BstYI
BstZ17I BtgI BtsI EaeI EarI Eco57MI Hin4I HincII
HindIII MfeI MmeI MspA1I NcoI NheI NsiI NspI
PciI PmlI PvuII SfcI SmaI SmlI SpeI SphI
StyI TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaBI
BsaHI BsaWI BsaXI BseYI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MluI MscI MslI NaeI NarI NdeI NgoMIV NotI
NruI PacI PasI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XhoI ZraI