Restriction map for
pFN21AE2149
(Linear) (Six-base) MAPSORT of: KIEE2149.seq Check: 910 from: 1 to: 1821
KIEE2149.orf
With 182 enzymes: SgfI *
March 22, 2012 15:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 59 1155 1422 1821
Size: 59 1096 267 399
Fragments arranged by size:
1096 399 267 59
AccI GT'mk_AC
Cuts at: 0 410 1821
Size: 410 1411
Acc65I G'GTAC_C
Cuts at: 0 248 1821
Size: 248 1573
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 550 1214 1821
Size: 337 213 664 607
Fragments arranged by size:
664 607 337 213
AflIII A'CryG_T
Cuts at: 0 290 782 1821
Size: 290 492 1039
Fragments arranged by size:
1039 492 290
AleI CACnn'nnGTG
Cuts at: 0 293 838 1821
Size: 293 545 983
Fragments arranged by size:
983 545 293
AlwNI CAG_nnn'CTG
Cuts at: 0 144 742 1039 1237 1696 1821
Size: 144 598 297 198 459 125
Fragments arranged by size:
598 459 297 198 144 125
ApaI G_GGCC'C
Cuts at: 0 75 83 1400 1674 1821
Size: 75 8 1317 274 147
Fragments arranged by size:
1317 274 147 75 8
ApaLI G'TGCA_C
Cuts at: 0 1089 1821
Size: 1089 732
AvaI C'yCGr_G
Cuts at: 0 75 172 278 1476 1502 1821
Size: 75 97 106 1198 26 319
Fragments arranged by size:
1198 319 106 97 75 26
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 240 273 1821
Size: 240 33 1548
Fragments arranged by size:
1548 240 33
BanI G'GyrC_C
Cuts at: 0 47 118 248 832 850 875 1057 1506
Size: 47 71 130 584 18 25 182 449
Cuts at: 1506 1821
Size: 315
Fragments arranged by size:
584 449 315 182 130 71 47 25
18
BanII G_rGCy'C
Cuts at: 0 75 83 374 670 913 1181 1400 1674
Size: 75 8 291 296 243 268 219 274
Cuts at: 1674 1821
Size: 147
Fragments arranged by size:
296 291 274 268 243 219 147 75
8
BbeI G_GCGC'C
Cuts at: 0 879 1821
Size: 879 942
BbvCI CC'TCA_GC
Cuts at: 0 1577 1821
Size: 1577 244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 636 670 883 917 1821
Size: 636 34 213 34 904
Fragments arranged by size:
904 636 213 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 45 1342 1423 1735 1821
Size: 45 1297 81 312 86
Fragments arranged by size:
1297 312 86 81 45
BlpI GC'TnA_GC
Cuts at: 0 550 701 967 1238 1557 1821
Size: 550 151 266 271 319 264
Fragments arranged by size:
550 319 271 266 264 151
Bme1580I G_kGCm'C
Cuts at: 0 75 83 121 173 229 782 1033 1093
Size: 75 8 38 52 56 553 251 60
Cuts at: 1093 1190 1217 1355 1400 1469 1511 1638 1674
Size: 97 27 138 45 69 42 127 36
Cuts at: 1674 1821
Size: 147
Fragments arranged by size:
553 251 147 138 127 97 75 69
60 56 52 45 42 38 36 27
8
BmrI ACTGGGnnnn_n'
Cuts at: 0 1342 1625 1725 1821
Size: 1342 283 100 96
Fragments arranged by size:
1342 283 100 96
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1788 1821
Size: 1788 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 910 1142 1270 1555 1691 1821
Size: 607 303 232 128 285 136 130
Fragments arranged by size:
607 303 285 232 136 130 128
Bpu10I CC'TnA_GC
Cuts at: 0 41 1577 1685 1821
Size: 41 1536 108 136
Fragments arranged by size:
1536 136 108 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 435 1821
Size: 435 1386
BsaAI yAC'GTr
Cuts at: 0 168 230 261 291 783 1821
Size: 168 62 31 30 492 1038
Fragments arranged by size:
1038 492 168 62 31 30
BsaHI Gr'CG_yC
Cuts at: 0 178 876 1821
Size: 178 698 945
Fragments arranged by size:
945 698 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 741 771 1243 1273 1347 1377 1821
Size: 741 30 472 30 74 30 444
Fragments arranged by size:
741 472 444 74 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 286 1267 1821
Size: 286 981 554
Fragments arranged by size:
981 554 286
BseYI C'CCAG_C
Cuts at: 0 57 770 927 1277 1565 1603 1701 1821
Size: 57 713 157 350 288 38 98 120
Fragments arranged by size:
713 350 288 157 120 98 57 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 1394 1821
Size: 252 1142 427
Fragments arranged by size:
1142 427 252
BsiEI CG_ry'CG
Cuts at: 0 629 863 1821
Size: 629 234 958
Fragments arranged by size:
958 629 234
BsiHKAI G_wGCw'C
Cuts at: 0 444 1093 1821
Size: 444 649 728
Fragments arranged by size:
728 649 444
BsmI GAATG_Cn'
Cuts at: 0 290 1821
Size: 290 1531
Bsp1286I G_dGCh'C
Cuts at: 0 75 83 121 173 229 374 444 670
Size: 75 8 38 52 56 145 70 226
Cuts at: 670 782 913 1033 1093 1181 1190 1217 1355
Size: 112 131 120 60 88 9 27 138
Cuts at: 1355 1400 1469 1511 1638 1674 1821
Size: 45 69 42 127 36 147
Fragments arranged by size:
226 147 145 138 131 127 120 112
88 75 70 69 60 56 52 45
42 38 36 27 9 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 59 100 1155 1422 1802 1821
Size: 59 41 1055 267 380 19
Fragments arranged by size:
1055 380 267 59 41 19
BsrBI CCG'CTC
Cuts at: 0 45 1821
Size: 45 1776
BsrFI r'CCGG_y
Cuts at: 0 1060 1821
Size: 1060 761
BstAPI GCAn_nnn'nTGC
Cuts at: 0 144 742 1821
Size: 144 598 1079
Fragments arranged by size:
1079 598 144
BstYI r'GATC_y
Cuts at: 0 399 506 1821
Size: 399 107 1315
Fragments arranged by size:
1315 399 107
BtgI C'CryG_G
Cuts at: 0 918 1391 1511 1821
Size: 918 473 120 310
Fragments arranged by size:
918 473 310 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 337 1257 1821
Size: 337 920 564
Fragments arranged by size:
920 564 337
BtsI GCAGTG_nn'
Cuts at: 0 854 1821
Size: 854 967
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1575 1610 1821
Size: 1575 35 211
Fragments arranged by size:
1575 211 35
DraIII CAC_nnn'GTG
Cuts at: 0 561 1821
Size: 561 1260
EaeI y'GGCC_r
Cuts at: 0 17 626 860 1268 1424 1821
Size: 17 609 234 408 156 397
Fragments arranged by size:
609 408 397 234 156 17
EagI C'GGCC_G
Cuts at: 0 626 860 1821
Size: 626 234 961
Fragments arranged by size:
961 626 234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 41 165 664 1821
Size: 41 124 499 1157
Fragments arranged by size:
1157 499 124 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 550 607 910 1142 1214 1270 1555
Size: 337 213 57 303 232 72 56 285
Cuts at: 1555 1691 1821
Size: 136 130
Fragments arranged by size:
337 303 285 232 213 136 130 72
57 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 460 1821
Size: 460 1361
EcoO109I rG'GnC_Cy
Cuts at: 0 71 72 79 80 711 1396 1397 1607
Size: 71 1 7 1 631 685 1 210
Cuts at: 1607 1670 1671 1821
Size: 63 1 150
Fragments arranged by size:
685 631 210 150 71 63 7 1
1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 450 1821
Size: 450 1371
HaeII r_GCGC'y
Cuts at: 0 879 1008 1129 1696 1821
Size: 879 129 121 567 125
Fragments arranged by size:
879 567 129 125 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 976 1008 1035 1067 1243 1275 1821
Size: 976 32 27 32 176 32 546
Fragments arranged by size:
976 546 176 32 32 32 27
HincII GTy'rAC
Cuts at: 0 411 1821
Size: 411 1410
KasI G'GCGC_C
Cuts at: 0 875 1821
Size: 875 946
KpnI G_GTAC'C
Cuts at: 0 252 1821
Size: 252 1569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1694 1799 1821
Size: 1694 105 22
Fragments arranged by size:
1694 105 22
MscI TGG'CCA
Cuts at: 0 1270 1821
Size: 1270 551
MslI CAynn'nnrTG
Cuts at: 0 293 838 1821
Size: 293 545 983
Fragments arranged by size:
983 545 293
MspA1I CmG'CkG
Cuts at: 0 23 503 742 897 966 1198 1821
Size: 23 480 239 155 69 232 623
Fragments arranged by size:
623 480 239 232 155 69 23
NaeI GCC'GGC
Cuts at: 0 1062 1821
Size: 1062 759
NarI GG'CG_CC
Cuts at: 0 876 1821
Size: 876 945
NgoMIV G'CCGG_C
Cuts at: 0 1060 1821
Size: 1060 761
NotI GC'GGCC_GC
Cuts at: 0 860 1821
Size: 860 961
NspI r_CATG'y
Cuts at: 0 1452 1821
Size: 1452 369
PasI CC'CwG_GG
Cuts at: 0 1639 1821
Size: 1639 182
PflMI CCAn_nnn'nTGG
Cuts at: 0 561 1821
Size: 561 1260
PmlI CAC'GTG
Cuts at: 0 230 261 291 783 1821
Size: 230 31 30 492 1038
Fragments arranged by size:
1038 492 230 31 30
PspOMI G'GGCC_C
Cuts at: 0 71 79 1396 1670 1821
Size: 71 8 1317 274 151
Fragments arranged by size:
1317 274 151 71 8
PstI C_TGCA'G
Cuts at: 0 97 849 1004 1197 1376 1821
Size: 97 752 155 193 179 445
Fragments arranged by size:
752 445 193 179 155 97
PvuII CAG'CTG
Cuts at: 0 742 897 966 1198 1821
Size: 742 155 69 232 623
Fragments arranged by size:
742 623 232 155 69
SalI G'TCGA_C
Cuts at: 0 409 1821
Size: 409 1412
SbfI CC_TGCA'GG
Cuts at: 0 97 1821
Size: 97 1724
SfcI C'TryA_G
Cuts at: 0 93 845 1000 1193 1372 1760 1821
Size: 93 752 155 193 179 388 61
Fragments arranged by size:
752 388 193 179 155 93 61
SfoI GGC'GCC
Cuts at: 0 877 1821
Size: 877 944
SmaI CCC'GGG
Cuts at: 0 77 280 1478 1504 1821
Size: 77 203 1198 26 317
Fragments arranged by size:
1198 317 203 77 26
StuI AGG'CCT
Cuts at: 0 39 1821
Size: 39 1782
StyI C'CwwG_G
Cuts at: 0 395 1271 1821
Size: 395 876 550
Fragments arranged by size:
876 550 395
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 345 1821
Size: 345 1476
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 275 307 741 773 1347 1379 1821
Size: 275 32 434 32 574 32 442
Fragments arranged by size:
574 442 434 275 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 402 1821
Size: 402 1419
XmaI C'CCGG_G
Cuts at: 0 75 278 1476 1502 1821
Size: 75 203 1198 26 319
Fragments arranged by size:
1198 319 203 75 26
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlwNI ApaI
ApaLI AvaI BaeI BanI BanII BbeI BbvCI BcgI
BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstYI BtgI
BtgZI BtsI CspCI DraIII EaeI EagI EciI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI
NspI PasI PflMI PmlI PspOMI PstI PvuII SalI
SbfI SfcI SfoI SmaI StuI StyI TaqII TstI
XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AlfI AloI
ApoI AscI AseI AsiSI AvrII BamHI BbsI BciVI
BclI BfrBI BglII BmgBI BmtI BpuEI BsaBI BsaWI
BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I ClaI DraI DrdI
EarI EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI MfeI MluI NcoI NdeI NheI NruI NsiI
PacI PciI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SanDI SapI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI
XmnI ZraI