(Linear) (Six-base) MAPSORT of: KIEE2138.seq  Check: 3895  from: 1  to: 387



KIEE2138.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:55  ..

AccI GT'mk_AC

Cuts at:      0     149     387 
   Size:        149     238
 
ApaLI G'TGCA_C

Cuts at:      0      91     387 
   Size:         91     296
 
AscI GG'CGCG_CC

Cuts at:      0     140     387 
   Size:        140     247
 
AvaI C'yCGr_G

Cuts at:      0     166     212     387 
   Size:        166      46     175

  Fragments arranged by size: 

                 175     166      46
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     232     387 
   Size:        232     155
 
Bme1580I G_kGCm'C

Cuts at:      0      95     387 
   Size:         95     292
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     211     235     387 
   Size:         55     156      24     152

  Fragments arranged by size: 

                 156     152      55      24
 
BseYI C'CCAG_C

Cuts at:      0     291     387 
   Size:        291      96
 
BsiHKAI G_wGCw'C

Cuts at:      0      95     387 
   Size:         95     292
 
Bsp1286I G_dGCh'C

Cuts at:      0      95     387 
   Size:         95     292
 
BsrBI CCG'CTC

Cuts at:      0     126     387 
   Size:        126     261
 
BsrFI r'CCGG_y

Cuts at:      0     136     387 
   Size:        136     251
 
BssHII G'CGCG_C

Cuts at:      0     140     387 
   Size:        140     247
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      99     387 
   Size:         99     288
 
BstYI r'GATC_y

Cuts at:      0     185     387 
   Size:        185     202
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     291     387 
   Size:        291      96
 
EaeI y'GGCC_r

Cuts at:      0     257     387 
   Size:        257     130
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     211     235     387 
   Size:         55     156      24     152

  Fragments arranged by size: 

                 156     152      55      24
 
FspI TGC'GCA

Cuts at:      0     106     387 
   Size:        106     281
 
MscI TGG'CCA

Cuts at:      0     259     387 
   Size:        259     128
 
MspA1I CmG'CkG

Cuts at:      0     255     387 
   Size:        255     132
 
NaeI GCC'GGC

Cuts at:      0     138     387 
   Size:        138     249
 
NgoMIV G'CCGG_C

Cuts at:      0     136     387 
   Size:        136     251
 
PasI CC'CwG_GG

Cuts at:      0     312     387 
   Size:        312      75
 
PfoI T'CCnGG_A

Cuts at:      0     188     387 
   Size:        188     199
 
PspXI vC'TCGA_Gb

Cuts at:      0     166     387 
   Size:        166     221
 
PvuII CAG'CTG

Cuts at:      0     255     387 
   Size:        255     132
 
SmlI C'TyrA_G

Cuts at:      0     166     387 
   Size:        166     221
 
StyI C'CwwG_G

Cuts at:      0      38     387 
   Size:         38     349
 
XhoI C'TCGA_G

Cuts at:      0     166     387 
   Size:        166     221
 

 Enzymes that do cut:

     AccI    ApaLI     AscI     AvaI     BbsI Bme1580I     BpmI    BseYI
  BsiHKAI Bsp1286I    BsrBI    BsrFI   BssHII   BstAPI    BstYI    BtgZI
     EaeI  Eco57MI     FspI     MscI   MspA1I     NaeI   NgoMIV     PasI
     PfoI    PspXI    PvuII     SmlI     StyI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrGI    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MslI     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI