Restriction map for
pFN21AE2132
(Linear) (Six-base) MAPSORT of: KIEE2132.seq Check: 3592 from: 1 to: 2589
KIEE2132.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AccI GT'mk_AC
Cuts at: 0 16 1232 2144 2589
Size: 16 1216 912 445
Fragments arranged by size:
1216 912 445 16
Acc65I G'GTAC_C
Cuts at: 0 1537 2589
Size: 1537 1052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 493 1045 1373 1429 2326 2426 2589
Size: 215 278 552 328 56 897 100 163
Fragments arranged by size:
897 552 328 278 215 163 100 56
AflII C'TTAA_G
Cuts at: 0 1345 2589
Size: 1345 1244
AflIII A'CryG_T
Cuts at: 0 1332 1565 1595 2589
Size: 1332 233 30 994
Fragments arranged by size:
1332 994 233 30
AleI CACnn'nnGTG
Cuts at: 0 331 2589
Size: 331 2258
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 200 1697 2110 2589
Size: 200 1497 413 479
Fragments arranged by size:
1497 479 413 200
AlwNI CAG_nnn'CTG
Cuts at: 0 1557 1785 2566 2589
Size: 1557 228 781 23
Fragments arranged by size:
1557 781 228 23
ApoI r'AATT_y
Cuts at: 0 660 1436 1495 2589
Size: 660 776 59 1094
Fragments arranged by size:
1094 776 660 59
AseI AT'TA_AT
Cuts at: 0 599 2209 2589
Size: 599 1610 380
Fragments arranged by size:
1610 599 380
AvaI C'yCGr_G
Cuts at: 0 21 290 889 938 2589
Size: 21 269 599 49 1651
Fragments arranged by size:
1651 599 269 49 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1521 1554 2589
Size: 1521 33 1035
Fragments arranged by size:
1521 1035 33
BanI G'GyrC_C
Cuts at: 0 1537 2589
Size: 1537 1052
BanII G_rGCy'C
Cuts at: 0 184 358 974 1612 2042 2589
Size: 184 174 616 638 430 547
Fragments arranged by size:
638 616 547 430 184 174
BbsI GAAGACnn'nnnn_
Cuts at: 0 805 836 1082 2578 2589
Size: 805 31 246 1496 11
Fragments arranged by size:
1496 805 246 31 11
BclI T'GATC_A
Cuts at: 0 866 1052 2589
Size: 866 186 1537
Fragments arranged by size:
1537 866 186
BfrBI ATG'CAT
Cuts at: 0 225 2589
Size: 225 2364
BglI GCCn_nnn'nGGC
Cuts at: 0 32 1089 2122 2589
Size: 32 1057 1033 467
Fragments arranged by size:
1057 1033 467 32
BlpI GC'TnA_GC
Cuts at: 0 2217 2589
Size: 2217 372
Bme1580I G_kGCm'C
Cuts at: 0 328 930 2538 2589
Size: 328 602 1608 51
Fragments arranged by size:
1608 602 328 51
BmgBI CAC'GTC
Cuts at: 0 198 1598 2589
Size: 198 1400 991
Fragments arranged by size:
1400 991 198
BmrI ACTGGGnnnn_n'
Cuts at: 0 968 2576 2589
Size: 968 1608 13
Fragments arranged by size:
1608 968 13
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 1596 2589
Size: 185 1411 993
Fragments arranged by size:
1411 993 185
BsaAI yAC'GTr
Cuts at: 0 1566 1638 2589
Size: 1566 72 951
Fragments arranged by size:
1566 951 72
BsaBI GATnn'nnATC
Cuts at: 0 1513 2589
Size: 1513 1076
BsaHI Gr'CG_yC
Cuts at: 0 13 67 1980 2589
Size: 13 54 1913 609
Fragments arranged by size:
1913 609 54 13
BsaWI w'CCGG_w
Cuts at: 0 1071 2589
Size: 1071 1518
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1012 1042 2589
Size: 1012 30 1547
Fragments arranged by size:
1547 1012 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2035 2047 2050 2589
Size: 2035 12 3 539
Fragments arranged by size:
2035 539 12 3
BseYI C'CCAG_C
Cuts at: 0 283 878 1550 1801 2589
Size: 283 595 672 251 788
Fragments arranged by size:
788 672 595 283 251
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 1637 2589
Size: 344 1293 952
Fragments arranged by size:
1293 952 344
BsiEI CG_ry'CG
Cuts at: 0 960 2589
Size: 960 1629
BsiHKAI G_wGCw'C
Cuts at: 0 1686 2042 2589
Size: 1686 356 547
Fragments arranged by size:
1686 547 356
BsiWI C'GTAC_G
Cuts at: 0 1634 2589
Size: 1634 955
BsmI GAATG_Cn'
Cuts at: 0 225 2589
Size: 225 2364
BsmBI CGTCTCn'nnnn_
Cuts at: 0 99 2589
Size: 99 2490
Bsp1286I G_dGCh'C
Cuts at: 0 184 328 358 930 974 1612 1686 2042
Size: 184 144 30 572 44 638 74 356
Cuts at: 2042 2538 2589
Size: 496 51
Fragments arranged by size:
638 572 496 356 184 144 74 51
44 30
BspEI T'CCGG_A
Cuts at: 0 1071 2589
Size: 1071 1518
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 182 2589
Size: 182 2407
BsrBI CCG'CTC
Cuts at: 0 1028 2589
Size: 1028 1561
BsrDI GCAATG_nn'
Cuts at: 0 962 1790 2069 2589
Size: 962 828 279 520
Fragments arranged by size:
962 828 520 279
BsrFI r'CCGG_y
Cuts at: 0 38 2589
Size: 38 2551
BsrGI T'GTAC_A
Cuts at: 0 665 1568 2589
Size: 665 903 1021
Fragments arranged by size:
1021 903 665
BssHII G'CGCG_C
Cuts at: 0 1825 2589
Size: 1825 764
BstAPI GCAn_nnn'nTGC
Cuts at: 0 150 198 2533 2589
Size: 150 48 2335 56
Fragments arranged by size:
2335 150 56 48
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2271 2589
Size: 2271 318
BstYI r'GATC_y
Cuts at: 0 381 2589
Size: 381 2208
Bsu36I CC'TnA_GG
Cuts at: 0 1958 2589
Size: 1958 631
BtgI C'CryG_G
Cuts at: 0 30 131 954 2098 2589
Size: 30 101 823 1144 491
Fragments arranged by size:
1144 823 491 101 30
BtsI GCAGTG_nn'
Cuts at: 0 1628 2527 2589
Size: 1628 899 62
Fragments arranged by size:
1628 899 62
ClaI AT'CG_AT
Cuts at: 0 1718 2589
Size: 1718 871
DraI TTT'AAA
Cuts at: 0 623 2589
Size: 623 1966
DraIII CAC_nnn'GTG
Cuts at: 0 2533 2589
Size: 2533 56
DrdI GACnn_nn'nnGTC
Cuts at: 0 718 2589
Size: 718 1871
EaeI y'GGCC_r
Cuts at: 0 957 2589
Size: 957 1632
EagI C'GGCC_G
Cuts at: 0 957 2589
Size: 957 1632
EarI CTCTTCn'nnn_
Cuts at: 0 1039 2270 2589
Size: 1039 1231 319
Fragments arranged by size:
1231 1039 319
EcoICRI GAG'CTC
Cuts at: 0 2040 2589
Size: 2040 549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 215 493 1045 1373 1429 1596 2326
Size: 185 30 278 552 328 56 167 730
Cuts at: 2326 2426 2589
Size: 100 163
Fragments arranged by size:
730 552 328 278 185 167 163 100
56 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1974 2589
Size: 1974 615
EcoO109I rG'GnC_Cy
Cuts at: 0 369 2589
Size: 369 2220
FspI TGC'GCA
Cuts at: 0 191 2589
Size: 191 2398
HaeII r_GCGC'y
Cuts at: 0 990 2589
Size: 990 1599
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 73 105 112 144 340 372 373 405
Size: 73 32 7 32 196 32 1 32
Cuts at: 405 662 694 1668 1700 1797 1829 2589
Size: 257 32 974 32 97 32 760
Fragments arranged by size:
974 760 257 196 97 73 32 32
32 32 32 32 32 7 1
HincII GTy'rAC
Cuts at: 0 17 1137 2589
Size: 17 1120 1452
Fragments arranged by size:
1452 1120 17
HindIII A'AGCT_T
Cuts at: 0 1057 2419 2589
Size: 1057 1362 170
Fragments arranged by size:
1362 1057 170
KpnI G_GTAC'C
Cuts at: 0 1541 2589
Size: 1541 1048
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 2589
Size: 727 1862
MslI CAynn'nnrTG
Cuts at: 0 331 1247 2053 2092 2269 2589
Size: 331 916 806 39 177 320
Fragments arranged by size:
916 806 331 320 177 39
MspA1I CmG'CkG
Cuts at: 0 32 857 882 956 1554 1665 1905 2100
Size: 32 825 25 74 598 111 240 195
Cuts at: 2100 2589
Size: 489
Fragments arranged by size:
825 598 489 240 195 111 74 32
25
NaeI GCC'GGC
Cuts at: 0 40 2589
Size: 40 2549
NgoMIV G'CCGG_C
Cuts at: 0 38 2589
Size: 38 2551
NotI GC'GGCC_GC
Cuts at: 0 957 2589
Size: 957 1632
NsiI A_TGCA'T
Cuts at: 0 227 2589
Size: 227 2362
NspI r_CATG'y
Cuts at: 0 485 582 1083 2589
Size: 485 97 501 1506
Fragments arranged by size:
1506 501 485 97
PasI CC'CwG_GG
Cuts at: 0 1583 2589
Size: 1583 1006
PflMI CCAn_nnn'nTGG
Cuts at: 0 313 1008 2589
Size: 313 695 1581
Fragments arranged by size:
1581 695 313
PfoI T'CCnGG_A
Cuts at: 0 1357 2042 2589
Size: 1357 685 547
Fragments arranged by size:
1357 685 547
PshAI GACnn'nnGTC
Cuts at: 0 1979 2589
Size: 1979 610
PstI C_TGCA'G
Cuts at: 0 179 389 1089 1562 1676 2589
Size: 179 210 700 473 114 913
Fragments arranged by size:
913 700 473 210 179 114
PvuII CAG'CTG
Cuts at: 0 882 1554 1665 1905 2589
Size: 882 672 111 240 684
Fragments arranged by size:
882 684 672 240 111
SacI G_AGCT'C
Cuts at: 0 2042 2589
Size: 2042 547
SacII CC_GC'GG
Cuts at: 0 33 957 2101 2589
Size: 33 924 1144 488
Fragments arranged by size:
1144 924 488 33
SalI G'TCGA_C
Cuts at: 0 15 2589
Size: 15 2574
SbfI CC_TGCA'GG
Cuts at: 0 1089 2589
Size: 1089 1500
SexAI A'CCwGG_T
Cuts at: 0 1994 2589
Size: 1994 595
SfcI C'TryA_G
Cuts at: 0 175 385 1085 1558 1672 2589
Size: 175 210 700 473 114 917
Fragments arranged by size:
917 700 473 210 175 114
SmaI CCC'GGG
Cuts at: 0 940 2589
Size: 940 1649
SmlI C'TyrA_G
Cuts at: 0 1345 2589
Size: 1345 1244
SnaBI TAC'GTA
Cuts at: 0 1638 2589
Size: 1638 951
SphI G_CATG'C
Cuts at: 0 485 2589
Size: 485 2104
StuI AGG'CCT
Cuts at: 0 345 904 2589
Size: 345 559 1685
Fragments arranged by size:
1685 559 345
StyI C'CwwG_G
Cuts at: 0 1394 2589
Size: 1394 1195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1015 2589
Size: 1015 1574
TatI w'GTAC_w
Cuts at: 0 665 1568 1592 1744 2006 2589
Size: 665 903 24 152 262 583
Fragments arranged by size:
903 665 583 262 152 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1012 1044 2589
Size: 1012 32 1545
Fragments arranged by size:
1545 1012 32
Tth111I GACn'n_nGTC
Cuts at: 0 1141 1785 2589
Size: 1141 644 804
Fragments arranged by size:
1141 804 644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 858 1005 1597 2589
Size: 858 147 592 992
Fragments arranged by size:
992 858 592 147
XmaI C'CCGG_G
Cuts at: 0 938 2589
Size: 938 1651
XmnI GAAnn'nnTTC
Cuts at: 0 494 698 2178 2477 2589
Size: 494 204 1480 299 112
Fragments arranged by size:
1480 494 299 204 112
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AleI AlfI AlwNI
ApoI AseI AvaI BaeI BanI BanII BbsI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BpmI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstXI BstYI Bsu36I
BtgI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
HincII HindIII KpnI MmeI MslI MspA1I NaeI NgoMIV
NotI NsiI NspI PasI PflMI PfoI PshAI PstI
PvuII SacI SacII SalI SbfI SexAI SfcI SmaI
SmlI SnaBI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AloI ApaI
ApaLI AscI AsiSI AvrII BamHI BbeI BbvCI BcgI
BciVI BglII BmtI BplI Bpu10I BpuEI BsaI BspHI
BssSI BstBI BstEII BstZ17I BtgZI CspCI EciI EcoRI
EcoRV FalI FseI FspAI HpaI KasI MfeI MluI
MscI NarI NcoI NdeI NheI NruI PacI PciI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PvuI RsrII SanDI SapI ScaI SfiI SfoI SgfI
SgrAI SpeI SrfI SspI SwaI XbaI XhoI ZraI