Restriction map for
pFN21AE2106
(Linear) (Six-base) MAPSORT of: KIEE2106.seq Check: 9969 from: 1 to: 2682
KIEE2106.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 377 713 2053 2682
Size: 180 197 336 1340 629
Fragments arranged by size:
1340 629 336 197 180
AfeI AGC'GCT
Cuts at: 0 1192 2682
Size: 1192 1490
AflII C'TTAA_G
Cuts at: 0 208 2682
Size: 208 2474
AflIII A'CryG_T
Cuts at: 0 35 2682
Size: 35 2647
AleI CACnn'nnGTG
Cuts at: 0 2159 2682
Size: 2159 523
AlwNI CAG_nnn'CTG
Cuts at: 0 1131 1659 2226 2339 2682
Size: 1131 528 567 113 343
Fragments arranged by size:
1131 567 528 343 113
ApaI G_GGCC'C
Cuts at: 0 1952 2614 2682
Size: 1952 662 68
Fragments arranged by size:
1952 662 68
ApaLI G'TGCA_C
Cuts at: 0 2630 2682
Size: 2630 52
ApoI r'AATT_y
Cuts at: 0 1454 2377 2682
Size: 1454 923 305
Fragments arranged by size:
1454 923 305
AvaI C'yCGr_G
Cuts at: 0 925 1696 2607 2682
Size: 925 771 911 75
Fragments arranged by size:
925 911 771 75
BamHI G'GATC_C
Cuts at: 0 1955 2507 2682
Size: 1955 552 175
Fragments arranged by size:
1955 552 175
BanI G'GyrC_C
Cuts at: 0 166 1117 1445 1916 2419 2682
Size: 166 951 328 471 503 263
Fragments arranged by size:
951 503 471 328 263 166
BanII G_rGCy'C
Cuts at: 0 17 1317 1403 1820 1952 1990 2010 2614
Size: 17 1300 86 417 132 38 20 604
Cuts at: 2614 2682
Size: 68
Fragments arranged by size:
1300 604 417 132 86 68 38 20
17
BbeI G_GCGC'C
Cuts at: 0 1920 2682
Size: 1920 762
BbsI GAAGACnn'nnnn_
Cuts at: 0 130 197 701 937 2021 2682
Size: 130 67 504 236 1084 661
Fragments arranged by size:
1084 661 504 236 130 67
BclI T'GATC_A
Cuts at: 0 1542 1661 2682
Size: 1542 119 1021
Fragments arranged by size:
1542 1021 119
BglI GCCn_nnn'nGGC
Cuts at: 0 1570 2682
Size: 1570 1112
BglII A'GATC_T
Cuts at: 0 1265 2682
Size: 1265 1417
Bme1580I G_kGCm'C
Cuts at: 0 564 1856 1952 2614 2634 2682
Size: 564 1292 96 662 20 48
Fragments arranged by size:
1292 662 564 96 48 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 1486 2571 2682
Size: 1486 1085 111
Fragments arranged by size:
1486 1085 111
BmtI G_CTAG'C
Cuts at: 0 868 2682
Size: 868 1814
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1592 2682
Size: 1592 1090
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 979 1042 1153 1207 1576 2104 2682
Size: 931 48 63 111 54 369 528 578
Fragments arranged by size:
931 578 528 369 111 63 54 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1767 2682
Size: 1767 915
BsaI GGTCTCn'nnnn_
Cuts at: 0 2084 2682
Size: 2084 598
BsaAI yAC'GTr
Cuts at: 0 36 2682
Size: 36 2646
BsaHI Gr'CG_yC
Cuts at: 0 1294 1917 2682
Size: 1294 623 765
Fragments arranged by size:
1294 765 623
BsaWI w'CCGG_w
Cuts at: 0 917 1820 2504 2682
Size: 917 903 684 178
Fragments arranged by size:
917 903 684 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 58 88 2682
Size: 58 30 2594
Fragments arranged by size:
2594 58 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 85 1171 1512 1660 1894 1987 2557
Size: 82 3 1086 341 148 234 93 570
Cuts at: 2557 2682
Size: 125
Fragments arranged by size:
1086 570 341 234 148 125 93 82
3
BseYI C'CCAG_C
Cuts at: 0 99 2682
Size: 99 2583
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 1024 2682
Size: 43 981 1658
Fragments arranged by size:
1658 981 43
BsiHKAI G_wGCw'C
Cuts at: 0 1403 1820 1990 2048 2156 2634 2682
Size: 1403 417 170 58 108 478 48
Fragments arranged by size:
1403 478 417 170 108 58 48
BsiWI C'GTAC_G
Cuts at: 0 740 2682
Size: 740 1942
BsmBI CGTCTCn'nnnn_
Cuts at: 0 750 2455 2682
Size: 750 1705 227
Fragments arranged by size:
1705 750 227
Bsp1286I G_dGCh'C
Cuts at: 0 17 564 1317 1403 1820 1856 1952 1990
Size: 17 547 753 86 417 36 96 38
Cuts at: 1990 2010 2048 2156 2614 2634 2682
Size: 20 38 108 458 20 48
Fragments arranged by size:
753 547 458 417 108 96 86 48
38 38 36 20 20 17
BspEI T'CCGG_A
Cuts at: 0 1820 2504 2682
Size: 1820 684 178
Fragments arranged by size:
1820 684 178
BspHI T'CATG_A
Cuts at: 0 734 2322 2450 2682
Size: 734 1588 128 232
Fragments arranged by size:
1588 734 232 128
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 95 2478 2682
Size: 95 2383 204
Fragments arranged by size:
2383 204 95
BsrBI CCG'CTC
Cuts at: 0 1713 2682
Size: 1713 969
BsrDI GCAATG_nn'
Cuts at: 0 381 2682
Size: 381 2301
BsrFI r'CCGG_y
Cuts at: 0 163 578 2682
Size: 163 415 2104
Fragments arranged by size:
2104 415 163
BssSI C'ACGA_G
Cuts at: 0 1237 1330 1849 2682
Size: 1237 93 519 833
Fragments arranged by size:
1237 833 519 93
BstAPI GCAn_nnn'nTGC
Cuts at: 0 945 1269 1851 2682
Size: 945 324 582 831
Fragments arranged by size:
945 831 582 324
BstYI r'GATC_y
Cuts at: 0 1265 1611 1649 1955 2222 2403 2507 2682
Size: 1265 346 38 306 267 181 104 175
Fragments arranged by size:
1265 346 306 267 181 175 104 38
Bsu36I CC'TnA_GG
Cuts at: 0 154 2682
Size: 154 2528
BtgI C'CryG_G
Cuts at: 0 644 1808 2351 2682
Size: 644 1164 543 331
Fragments arranged by size:
1164 644 543 331
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1374 2175 2478 2682
Size: 1374 801 303 204
Fragments arranged by size:
1374 801 303 204
BtsI GCAGTG_nn'
Cuts at: 0 131 1664 2477 2625 2682
Size: 131 1533 813 148 57
Fragments arranged by size:
1533 813 148 131 57
EaeI y'GGCC_r
Cuts at: 0 641 1727 2682
Size: 641 1086 955
Fragments arranged by size:
1086 955 641
EarI CTCTTCn'nnn_
Cuts at: 0 1489 2658 2682
Size: 1489 1169 24
Fragments arranged by size:
1489 1169 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1717 2554 2682
Size: 1717 837 128
Fragments arranged by size:
1717 837 128
EcoICRI GAG'CTC
Cuts at: 0 1401 1818 1988 2682
Size: 1401 417 170 694
Fragments arranged by size:
1401 694 417 170
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 377 713 931 979 1042 1153 1207
Size: 180 197 336 218 48 63 111 54
Cuts at: 1207 1576 2053 2104 2682
Size: 369 477 51 578
Fragments arranged by size:
578 477 369 336 218 197 180 111
63 54 51 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1125 1876 2539 2682
Size: 1125 751 663 143
Fragments arranged by size:
1125 751 663 143
EcoO109I rG'GnC_Cy
Cuts at: 0 1949 2112 2682
Size: 1949 163 570
Fragments arranged by size:
1949 570 163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 565 2682
Size: 565 2117
FspI TGC'GCA
Cuts at: 0 1670 2682
Size: 1670 1012
HaeII r_GCGC'y
Cuts at: 0 356 1194 1604 1920 2682
Size: 356 838 410 316 762
Fragments arranged by size:
838 762 410 356 316
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1273 1279 1305 1311 1641 1673 1702 1734
Size: 1273 6 26 6 330 32 29 32
Cuts at: 1734 2682
Size: 948
Fragments arranged by size:
1273 948 330 32 32 29 26 6
6
HincII GTy'rAC
Cuts at: 0 300 2682
Size: 300 2382
HindIII A'AGCT_T
Cuts at: 0 305 604 2682
Size: 305 299 2078
Fragments arranged by size:
2078 305 299
KasI G'GCGC_C
Cuts at: 0 1916 2682
Size: 1916 766
MfeI C'AATT_G
Cuts at: 0 1587 2682
Size: 1587 1095
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1085 2145 2682
Size: 665 420 1060 537
Fragments arranged by size:
1060 665 537 420
MscI TGG'CCA
Cuts at: 0 643 1729 2682
Size: 643 1086 953
Fragments arranged by size:
1086 953 643
MslI CAynn'nnrTG
Cuts at: 0 2159 2682
Size: 2159 523
MspA1I CmG'CkG
Cuts at: 0 793 1020 2031 2682
Size: 793 227 1011 651
Fragments arranged by size:
1011 793 651 227
NaeI GCC'GGC
Cuts at: 0 165 2682
Size: 165 2517
NarI GG'CG_CC
Cuts at: 0 1917 2682
Size: 1917 765
NcoI C'CATG_G
Cuts at: 0 644 1808 2351 2682
Size: 644 1164 543 331
Fragments arranged by size:
1164 644 543 331
NgoMIV G'CCGG_C
Cuts at: 0 163 2682
Size: 163 2519
NheI G'CTAG_C
Cuts at: 0 864 2682
Size: 864 1818
NspI r_CATG'y
Cuts at: 0 1900 2682
Size: 1900 782
PasI CC'CwG_GG
Cuts at: 0 390 2682
Size: 390 2292
PfoI T'CCnGG_A
Cuts at: 0 62 92 968 2105 2682
Size: 62 30 876 1137 577
Fragments arranged by size:
1137 876 577 62 30
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1473 1505 2682
Size: 1473 32 1177
Fragments arranged by size:
1473 1177 32
PshAI GACnn'nnGTC
Cuts at: 0 742 2682
Size: 742 1940
PspOMI G'GGCC_C
Cuts at: 0 1948 2610 2682
Size: 1948 662 72
Fragments arranged by size:
1948 662 72
PstI C_TGCA'G
Cuts at: 0 1046 1277 1877 2682
Size: 1046 231 600 805
Fragments arranged by size:
1046 805 600 231
PvuII CAG'CTG
Cuts at: 0 1020 2031 2682
Size: 1020 1011 651
Fragments arranged by size:
1020 1011 651
SacI G_AGCT'C
Cuts at: 0 1403 1820 1990 2682
Size: 1403 417 170 692
Fragments arranged by size:
1403 692 417 170
SbfI CC_TGCA'GG
Cuts at: 0 1877 2682
Size: 1877 805
ScaI AGT'ACT
Cuts at: 0 2581 2682
Size: 2581 101
SfcI C'TryA_G
Cuts at: 0 1042 1273 1873 2682
Size: 1042 231 600 809
Fragments arranged by size:
1042 809 600 231
SfoI GGC'GCC
Cuts at: 0 1918 2682
Size: 1918 764
SmlI C'TyrA_G
Cuts at: 0 208 1782 2682
Size: 208 1574 900
Fragments arranged by size:
1574 900 208
SphI G_CATG'C
Cuts at: 0 1900 2682
Size: 1900 782
SspI AAT'ATT
Cuts at: 0 633 2682
Size: 633 2049
StuI AGG'CCT
Cuts at: 0 1228 2682
Size: 1228 1454
StyI C'CwwG_G
Cuts at: 0 549 644 998 1808 2351 2682
Size: 549 95 354 810 543 331
Fragments arranged by size:
810 549 543 354 331 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 184 1011 1487 2266 2356 2682
Size: 184 827 476 779 90 326
Fragments arranged by size:
827 779 476 326 184 90
TatI w'GTAC_w
Cuts at: 0 26 53 2579 2682
Size: 26 27 2526 103
Fragments arranged by size:
2526 103 27 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 58 90 2682
Size: 58 32 2592
Fragments arranged by size:
2592 58 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2208 2682
Size: 2208 474
XmnI GAAnn'nnTTC
Cuts at: 0 127 887 2269 2682
Size: 127 760 1382 413
Fragments arranged by size:
1382 760 413 127
Enzymes that do cut:
AcuI AfeI AflII AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BclI
BglI BglII Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstYI Bsu36I BtgI BtgZI
BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII HindIII KasI MfeI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PfoI PpiI PshAI PspOMI PstI
PvuII SacI SbfI ScaI SfcI SfoI SmlI SphI
SspI StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AhdI AlfI
AloI AscI AseI AsiSI AvrII BaeI BbvCI BcgI
BciVI BfrBI BlpI BmgBI Bpu10I BsaBI BsiEI BsmI
BsrGI BssHII BstBI BstEII BstXI BstZ17I ClaI CspCI
DraI DraIII DrdI EagI EcoRI EcoRV FseI FspAI
HpaI KpnI MluI NdeI NotI NruI NsiI PacI
PciI PflMI PmeI PmlI PpuMI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I
XbaI XhoI XmaI ZraI