Restriction map for
pFN21AE2105
(Linear) (Six-base) MAPSORT of: KIEE2105.seq Check: 5306 from: 1 to: 2517
KIEE2105.orf
With 182 enzymes: SgfI *
March 4, 2011 18:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 231 2334 2517
Size: 231 2103 183
Fragments arranged by size:
2103 231 183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 1549 1820 2517
Size: 207 1342 271 697
Fragments arranged by size:
1342 697 271 207
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 598 1873 2517
Size: 598 1275 644
Fragments arranged by size:
1275 644 598
AlwNI CAG_nnn'CTG
Cuts at: 0 246 390 2277 2517
Size: 246 144 1887 240
Fragments arranged by size:
1887 246 240 144
ApoI r'AATT_y
Cuts at: 0 129 784 859 1021 2082 2517
Size: 129 655 75 162 1061 435
Fragments arranged by size:
1061 655 435 162 129 75
BanI G'GyrC_C
Cuts at: 0 2097 2517
Size: 2097 420
BanII G_rGCy'C
Cuts at: 0 83 2517
Size: 83 2434
BbsI GAAGACnn'nnnn_
Cuts at: 0 1555 2332 2517
Size: 1555 777 185
Fragments arranged by size:
1555 777 185
BciVI GTATCCnnnnn_n'
Cuts at: 0 1599 2517
Size: 1599 918
BfrBI ATG'CAT
Cuts at: 0 119 2517
Size: 119 2398
BglII A'GATC_T
Cuts at: 0 1134 1212 2517
Size: 1134 78 1305
Fragments arranged by size:
1305 1134 78
BmrI ACTGGGnnnn_n'
Cuts at: 0 2067 2517
Size: 2067 450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1542 1543 1621 2404 2442 2517
Size: 1542 1 78 783 38 75
Fragments arranged by size:
1542 783 78 75 38 1
Bpu10I CC'TnA_GC
Cuts at: 0 181 301 2335 2517
Size: 181 120 2034 182
Fragments arranged by size:
2034 182 181 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1144 1173 2025 2517
Size: 1144 29 852 492
Fragments arranged by size:
1144 852 492 29
BsaI GGTCTCn'nnnn_
Cuts at: 0 57 2517
Size: 57 2460
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1516 1540 1546 1570 2517
Size: 1516 24 6 24 947
Fragments arranged by size:
1516 947 24 24 6
BseYI C'CCAG_C
Cuts at: 0 22 36 74 2213 2517
Size: 22 14 38 2139 304
Fragments arranged by size:
2139 304 38 22 14
BsiHKAI G_wGCw'C
Cuts at: 0 536 2517
Size: 536 1981
BsmI GAATG_Cn'
Cuts at: 0 1098 1290 2517
Size: 1098 192 1227
Fragments arranged by size:
1227 1098 192
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2418 2517
Size: 2418 99
Bsp1286I G_dGCh'C
Cuts at: 0 83 536 2517
Size: 83 453 1981
Fragments arranged by size:
1981 453 83
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 231 2334 2517
Size: 231 2103 183
Fragments arranged by size:
2103 231 183
BsrDI GCAATG_nn'
Cuts at: 0 1780 2150 2517
Size: 1780 370 367
Fragments arranged by size:
1780 370 367
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1851 2517
Size: 1851 666
BstXI CCAn_nnnn'nTGG
Cuts at: 0 74 174 2517
Size: 74 100 2343
Fragments arranged by size:
2343 100 74
BstYI r'GATC_y
Cuts at: 0 1134 1212 2517
Size: 1134 78 1305
Fragments arranged by size:
1305 1134 78
Bsu36I CC'TnA_GG
Cuts at: 0 196 2517
Size: 196 2321
BtgI C'CryG_G
Cuts at: 0 41 2517
Size: 41 2476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1068 1103 2517
Size: 1068 35 1414
Fragments arranged by size:
1414 1068 35
DraI TTT'AAA
Cuts at: 0 669 719 2517
Size: 669 50 1798
Fragments arranged by size:
1798 669 50
EaeI y'GGCC_r
Cuts at: 0 2147 2517
Size: 2147 370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 1542 1543 1549 1621 1820 2404 2442
Size: 207 1335 1 6 72 199 584 38
Cuts at: 2442 2517
Size: 75
Fragments arranged by size:
1335 584 207 199 75 72 38 6
1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2314 2517
Size: 2314 203
EcoO109I rG'GnC_Cy
Cuts at: 0 834 2517
Size: 834 1683
EcoRI G'AATT_C
Cuts at: 0 859 2517
Size: 859 1658
EcoRV GAT'ATC
Cuts at: 0 1470 2517
Size: 1470 1047
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1085 2517
Size: 1085 1432
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 174 206 1516 1548 2440 2472 2517
Size: 174 32 1310 32 892 32 45
Fragments arranged by size:
1310 892 174 45 32 32 32
HincII GTy'rAC
Cuts at: 0 1014 1519 2517
Size: 1014 505 998
Fragments arranged by size:
1014 998 505
HpaI GTT'AAC
Cuts at: 0 1014 2517
Size: 1014 1503
MfeI C'AATT_G
Cuts at: 0 275 2493 2517
Size: 275 2218 24
Fragments arranged by size:
2218 275 24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 678 1103 1561 2517
Size: 678 425 458 956
Fragments arranged by size:
956 678 458 425
MscI TGG'CCA
Cuts at: 0 2149 2517
Size: 2149 368
MslI CAynn'nnrTG
Cuts at: 0 1855 1903 2517
Size: 1855 48 614
Fragments arranged by size:
1855 614 48
MspA1I CmG'CkG
Cuts at: 0 2234 2517
Size: 2234 283
NcoI C'CATG_G
Cuts at: 0 41 2517
Size: 41 2476
NsiI A_TGCA'T
Cuts at: 0 121 2517
Size: 121 2396
NspI r_CATG'y
Cuts at: 0 1854 2517
Size: 1854 663
PasI CC'CwG_GG
Cuts at: 0 354 2517
Size: 354 2163
PflMI CCAn_nnn'nTGG
Cuts at: 0 771 2517
Size: 771 1746
PfoI T'CCnGG_A
Cuts at: 0 1448 1499 2517
Size: 1448 51 1018
Fragments arranged by size:
1448 1018 51
PsiI TTA'TAA
Cuts at: 0 983 1619 2517
Size: 983 636 898
Fragments arranged by size:
983 898 636
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2466 2498 2517
Size: 2466 32 19
Fragments arranged by size:
2466 32 19
PstI C_TGCA'G
Cuts at: 0 242 2315 2517
Size: 242 2073 202
Fragments arranged by size:
2073 242 202
SexAI A'CCwGG_T
Cuts at: 0 657 2517
Size: 657 1860
SfcI C'TryA_G
Cuts at: 0 213 238 1690 2124 2311 2517
Size: 213 25 1452 434 187 206
Fragments arranged by size:
1452 434 213 206 187 25
SmlI C'TyrA_G
Cuts at: 0 1123 1152 2040 2517
Size: 1123 29 888 477
Fragments arranged by size:
1123 888 477 29
SphI G_CATG'C
Cuts at: 0 1854 2517
Size: 1854 663
SspI AAT'ATT
Cuts at: 0 105 323 2517
Size: 105 218 2194
Fragments arranged by size:
2194 218 105
StyI C'CwwG_G
Cuts at: 0 41 2517
Size: 41 2476
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1899 2517
Size: 1899 618
TatI w'GTAC_w
Cuts at: 0 1747 2517
Size: 1747 770
Tth111I GACn'n_nGTC
Cuts at: 0 2398 2517
Size: 2398 119
XbaI T'CTAG_A
Cuts at: 0 420 528 828 1131 2517
Size: 420 108 300 303 1386
Fragments arranged by size:
1386 420 303 300 108
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApoI BanI BanII BbsI
BciVI BfrBI BglII BmrI BpmI Bpu10I BpuEI BsaI
BsaXI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI
BstAPI BstXI BstYI Bsu36I BtgI CspCI DraI EaeI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII
HpaI MfeI MmeI MscI MslI MspA1I NcoI NsiI
NspI PasI PflMI PfoI PsiI PsrI PstI SexAI
SfcI SmlI SphI SspI StyI TaqII TatI Tth111I
XbaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BclI
BglI BlpI Bme1580I BmgBI BmtI BplI BsaAI BsaBI
BsaHI BsaWI BseRI BsgI BsiEI BsiWI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgZI BtsI ClaI DraIII DrdI EagI EarI EciI
EcoICRI FseI FspI FspAI HaeII HindIII KasI KpnI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PciI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI StuI SwaI TstI XcmI XhoI
XmaI XmnI ZraI