Restriction map for
pFN21AE2091
(Linear) (Six-base) MAPSORT of: KIEE2091.seq Check: 4030 from: 1 to: 2295
KIEE2091.orf
With 182 enzymes: SgfI *
July 3, 2012 17:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 461 2295
Size: 461 1834
AccI GT'mk_AC
Cuts at: 0 1022 2295
Size: 1022 1273
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 1464 1987 2155 2295
Size: 784 680 523 168 140
Fragments arranged by size:
784 680 523 168 140
AfeI AGC'GCT
Cuts at: 0 256 1188 2295
Size: 256 932 1107
Fragments arranged by size:
1107 932 256
AflIII A'CryG_T
Cuts at: 0 1412 2295
Size: 1412 883
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1721 2295
Size: 1721 574
AlwNI CAG_nnn'CTG
Cuts at: 0 813 825 1506 2181 2295
Size: 813 12 681 675 114
Fragments arranged by size:
813 681 675 114 12
ApaI G_GGCC'C
Cuts at: 0 800 866 1614 2066 2262 2295
Size: 800 66 748 452 196 33
Fragments arranged by size:
800 748 452 196 66 33
ApaLI G'TGCA_C
Cuts at: 0 1558 2295
Size: 1558 737
ApoI r'AATT_y
Cuts at: 0 1000 2295
Size: 1000 1295
AvaI C'yCGr_G
Cuts at: 0 1606 1663 1711 1972 2050 2076 2229 2295
Size: 1606 57 48 261 78 26 153 66
Fragments arranged by size:
1606 261 153 78 66 57 48 26
BamHI G'GATC_C
Cuts at: 0 758 837 2295
Size: 758 79 1458
Fragments arranged by size:
1458 758 79
BanI G'GyrC_C
Cuts at: 0 282 595 1086 1668 2295
Size: 282 313 491 582 627
Fragments arranged by size:
627 582 491 313 282
BanII G_rGCy'C
Cuts at: 0 800 866 1614 1940 2066 2212 2241 2262
Size: 800 66 748 326 126 146 29 21
Cuts at: 2262 2295
Size: 33
Fragments arranged by size:
800 748 326 146 126 66 33 29
21
BbeI G_GCGC'C
Cuts at: 0 286 2295
Size: 286 2009
BbvCI CC'TCA_GC
Cuts at: 0 407 743 2295
Size: 407 336 1552
Fragments arranged by size:
1552 407 336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1324 1358 2057 2091 2295
Size: 1324 34 699 34 204
Fragments arranged by size:
1324 699 204 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1066 1917 2295
Size: 1066 851 378
Fragments arranged by size:
1066 851 378
BglI GCCn_nnn'nGGC
Cuts at: 0 1277 2295
Size: 1277 1018
BlpI GC'TnA_GC
Cuts at: 0 544 2295
Size: 544 1751
Bme1580I G_kGCm'C
Cuts at: 0 71 121 167 800 866 876 1562 1614
Size: 71 50 46 633 66 10 686 52
Cuts at: 1614 1673 2066 2262 2295
Size: 59 393 196 33
Fragments arranged by size:
686 633 393 196 71 66 59 52
50 46 33 10
BmgBI CAC'GTC
Cuts at: 0 2046 2295
Size: 2046 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 511 739 1033 1172 1336 1447 1474
Size: 286 225 228 294 139 164 111 27
Cuts at: 1474 1776 2295
Size: 302 519
Fragments arranged by size:
519 302 294 286 228 225 164 139
111 27
Bpu10I CC'TnA_GC
Cuts at: 0 407 743 2295
Size: 407 336 1552
Fragments arranged by size:
1552 407 336
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 1555 2295
Size: 91 1464 740
Fragments arranged by size:
1464 740 91
BsaI GGTCTCn'nnnn_
Cuts at: 0 473 1148 2086 2295
Size: 473 675 938 209
Fragments arranged by size:
938 675 473 209
BsaAI yAC'GTr
Cuts at: 0 1415 2295
Size: 1415 880
BsaHI Gr'CG_yC
Cuts at: 0 283 1681 1766 2038 2295
Size: 283 1398 85 272 257
Fragments arranged by size:
1398 283 272 257 85
BsaWI w'CCGG_w
Cuts at: 0 884 2033 2295
Size: 884 1149 262
Fragments arranged by size:
1149 884 262
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 580 1558 2010 2295
Size: 88 492 978 452 285
Fragments arranged by size:
978 492 452 285 88
BseYI C'CCAG_C
Cuts at: 0 114 1227 2295
Size: 114 1113 1068
Fragments arranged by size:
1113 1068 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 286 316 439 1369 1393 2201 2295
Size: 238 48 30 123 930 24 808 94
Fragments arranged by size:
930 808 238 123 94 48 30 24
BsiEI CG_ry'CG
Cuts at: 0 1526 1654 1844 2295
Size: 1526 128 190 451
Fragments arranged by size:
1526 451 190 128
BsiHKAI G_wGCw'C
Cuts at: 0 242 772 1460 1562 2051 2295
Size: 242 530 688 102 489 244
Fragments arranged by size:
688 530 489 244 242 102
BsmI GAATG_Cn'
Cuts at: 0 2101 2154 2295
Size: 2101 53 141
Fragments arranged by size:
2101 141 53
Bsp1286I G_dGCh'C
Cuts at: 0 71 121 167 242 772 800 866 876
Size: 71 50 46 75 530 28 66 10
Cuts at: 876 1460 1562 1614 1673 1940 2051 2066 2212
Size: 584 102 52 59 267 111 15 146
Cuts at: 2212 2241 2262 2295
Size: 29 21 33
Fragments arranged by size:
584 530 267 146 111 102 75 71
66 59 52 50 46 33 29 28
21 15 10
BspEI T'CCGG_A
Cuts at: 0 884 2033 2295
Size: 884 1149 262
Fragments arranged by size:
1149 884 262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 461 821 1220 1785 2295
Size: 461 360 399 565 510
Fragments arranged by size:
565 510 461 399 360
BsrBI CCG'CTC
Cuts at: 0 356 576 968 1277 2031 2295
Size: 356 220 392 309 754 264
Fragments arranged by size:
754 392 356 309 264 220
BsrFI r'CCGG_y
Cuts at: 0 1157 2295
Size: 1157 1138
BsrGI T'GTAC_A
Cuts at: 0 1775 2295
Size: 1775 520
BssHII G'CGCG_C
Cuts at: 0 222 2012 2014 2295
Size: 222 1790 2 281
Fragments arranged by size:
1790 281 222 2
BssSI C'ACGA_G
Cuts at: 0 70 1387 1516 1540 1992 2295
Size: 70 1317 129 24 452 303
Fragments arranged by size:
1317 452 303 129 70 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 813 825 1392 2181 2295
Size: 813 12 567 789 114
Fragments arranged by size:
813 789 567 114 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1926 2097 2295
Size: 1926 171 198
Fragments arranged by size:
1926 198 171
BstYI r'GATC_y
Cuts at: 0 758 837 1298 1502 2295
Size: 758 79 461 204 793
Fragments arranged by size:
793 758 461 204 79
BtgI C'CryG_G
Cuts at: 0 32 800 1946 2295
Size: 32 768 1146 349
Fragments arranged by size:
1146 768 349 32
BtsI GCAGTG_nn'
Cuts at: 0 2075 2295
Size: 2075 220
DraIII CAC_nnn'GTG
Cuts at: 0 1392 2295
Size: 1392 903
EaeI y'GGCC_r
Cuts at: 0 1159 1178 1278 1523 1651 1818 1841 2159
Size: 1159 19 100 245 128 167 23 318
Cuts at: 2159 2295
Size: 136
Fragments arranged by size:
1159 318 245 167 136 128 100 23
19
EagI C'GGCC_G
Cuts at: 0 1523 1651 1841 2295
Size: 1523 128 190 454
Fragments arranged by size:
1523 454 190 128
EarI CTCTTCn'nnn_
Cuts at: 0 688 2131 2295
Size: 688 1443 164
Fragments arranged by size:
1443 688 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2184 2295
Size: 2184 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 511 739 784 1033 1172 1336 1447
Size: 286 225 228 45 249 139 164 111
Cuts at: 1447 1464 1474 1776 1987 2155 2295
Size: 17 10 302 211 168 140
Fragments arranged by size:
302 286 249 228 225 211 168 164
140 139 111 45 17 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 562 2295
Size: 562 1733
EcoO109I rG'GnC_Cy
Cuts at: 0 701 810 862 863 906 1053 1610 1611
Size: 701 109 52 1 43 147 557 1
Cuts at: 1611 1797 1811 2258 2259 2295
Size: 186 14 447 1 36
Fragments arranged by size:
701 557 447 186 147 109 52 43
36 14 1 1 1
EcoRI G'AATT_C
Cuts at: 0 1000 2295
Size: 1000 1295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1494 2295
Size: 1494 801
FspI TGC'GCA
Cuts at: 0 202 1510 2295
Size: 202 1308 785
Fragments arranged by size:
1308 785 202
HaeII r_GCGC'y
Cuts at: 0 214 258 286 390 1144 1190 1272 1875
Size: 214 44 28 104 754 46 82 603
Cuts at: 1875 2192 2295
Size: 317 103
Fragments arranged by size:
754 603 317 214 104 103 82 46
44 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1290 1322 2228 2260 2295
Size: 1290 32 906 32 35
Fragments arranged by size:
1290 906 35 32 32
KasI G'GCGC_C
Cuts at: 0 282 2295
Size: 282 2013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1469 2295
Size: 1469 826
MscI TGG'CCA
Cuts at: 0 1180 1820 2161 2295
Size: 1180 640 341 134
Fragments arranged by size:
1180 640 341 134
MslI CAynn'nnrTG
Cuts at: 0 193 2095 2295
Size: 193 1902 200
Fragments arranged by size:
1902 200 193
MspA1I CmG'CkG
Cuts at: 0 822 1174 1259 1407 2295
Size: 822 352 85 148 888
Fragments arranged by size:
888 822 352 148 85
NarI GG'CG_CC
Cuts at: 0 283 2295
Size: 283 2012
NotI GC'GGCC_GC
Cuts at: 0 1651 2295
Size: 1651 644
PasI CC'CwG_GG
Cuts at: 0 867 2295
Size: 867 1428
PfoI T'CCnGG_A
Cuts at: 0 341 1424 2295
Size: 341 1083 871
Fragments arranged by size:
1083 871 341
PpuMI rG'GwC_Cy
Cuts at: 0 810 1053 1797 2295
Size: 810 243 744 498
Fragments arranged by size:
810 744 498 243
PspOMI G'GGCC_C
Cuts at: 0 796 862 1610 2062 2258 2295
Size: 796 66 748 452 196 37
Fragments arranged by size:
796 748 452 196 66 37
PspXI vC'TCGA_Gb
Cuts at: 0 1972 2050 2295
Size: 1972 78 245
Fragments arranged by size:
1972 245 78
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1573 1605 2295
Size: 1573 32 690
Fragments arranged by size:
1573 690 32
PstI C_TGCA'G
Cuts at: 0 821 1700 2003 2295
Size: 821 879 303 292
Fragments arranged by size:
879 821 303 292
PvuII CAG'CTG
Cuts at: 0 1407 2295
Size: 1407 888
SanDI GG'GwC_CC
Cuts at: 0 1053 2295
Size: 1053 1242
SapI GCTCTTCn'nnn_
Cuts at: 0 688 2131 2295
Size: 688 1443 164
Fragments arranged by size:
1443 688 164
ScaI AGT'ACT
Cuts at: 0 394 2295
Size: 394 1901
SexAI A'CCwGG_T
Cuts at: 0 1418 2295
Size: 1418 877
SfcI C'TryA_G
Cuts at: 0 817 1696 1999 2295
Size: 817 879 303 296
Fragments arranged by size:
879 817 303 296
SfoI GGC'GCC
Cuts at: 0 284 2295
Size: 284 2011
SmaI CCC'GGG
Cuts at: 0 1608 1665 2231 2295
Size: 1608 57 566 64
Fragments arranged by size:
1608 566 64 57
SmlI C'TyrA_G
Cuts at: 0 106 1570 1972 2050 2295
Size: 106 1464 402 78 245
Fragments arranged by size:
1464 402 245 106 78
StuI AGG'CCT
Cuts at: 0 1894 2295
Size: 1894 401
StyI C'CwwG_G
Cuts at: 0 1919 2295
Size: 1919 376
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 388 906 1141 2295
Size: 388 518 235 1154
Fragments arranged by size:
1154 518 388 235
TatI w'GTAC_w
Cuts at: 0 392 605 1775 2295
Size: 392 213 1170 520
Fragments arranged by size:
1170 520 392 213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 866 898 2295
Size: 866 32 1397
Fragments arranged by size:
1397 866 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 606 1813 2295
Size: 606 1207 482
Fragments arranged by size:
1207 606 482
XhoI C'TCGA_G
Cuts at: 0 1972 2050 2295
Size: 1972 78 245
Fragments arranged by size:
1972 245 78
XmaI C'CCGG_G
Cuts at: 0 1606 1663 2229 2295
Size: 1606 57 566 66
Fragments arranged by size:
1606 566 66 57
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BglI BlpI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstXI BstYI BtgI BtsI
DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NarI NotI PasI PfoI PpuMI PspOMI
PspXI PsrI PstI PvuII SanDI SapI ScaI SexAI
SfcI SfoI SmaI SmlI StuI StyI TaqII TatI
TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AloI
AscI AseI AsiSI AvrII BaeI BbsI BclI BfrBI
BglII BmrI BmtI BplI BsaBI BsaXI BsiWI BsmBI
BspHI BsrDI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EcoICRI EcoRV FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI
NgoMIV NheI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PvuI RsrII SacI
SacII SalI SbfI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XmnI ZraI