Restriction map for
pFN21AE2085
(Linear) (Six-base) MAPSORT of: KIEE2085.seq Check: 1742 from: 1 to: 2277
KIEE2085.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 663 2277
Size: 663 1614
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1633 1684 1806 2277
Size: 1633 51 122 471
Fragments arranged by size:
1633 471 122 51
AflIII A'CryG_T
Cuts at: 0 182 407 1908 2277
Size: 182 225 1501 369
Fragments arranged by size:
1501 369 225 182
AhdI GACnn_n'nnGTC
Cuts at: 0 2129 2277
Size: 2129 148
AleI CACnn'nnGTG
Cuts at: 0 478 2277
Size: 478 1799
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 496 1897 2277
Size: 496 1401 380
Fragments arranged by size:
1401 496 380
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1465 1497 2277
Size: 1465 32 780
Fragments arranged by size:
1465 780 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1005 1788 1890 2277
Size: 1005 783 102 387
Fragments arranged by size:
1005 783 387 102
ApaLI G'TGCA_C
Cuts at: 0 1621 2277
Size: 1621 656
ApoI r'AATT_y
Cuts at: 0 700 1762 2012 2170 2277
Size: 700 1062 250 158 107
Fragments arranged by size:
1062 700 250 158 107
AvaI C'yCGr_G
Cuts at: 0 247 2277
Size: 247 2030
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 949 982 2277
Size: 949 33 1295
Fragments arranged by size:
1295 949 33
BanII G_rGCy'C
Cuts at: 0 257 920 2277
Size: 257 663 1357
Fragments arranged by size:
1357 663 257
BbsI GAAGACnn'nnnn_
Cuts at: 0 338 374 2277
Size: 338 36 1903
Fragments arranged by size:
1903 338 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1672 1706 2277
Size: 1672 34 571
Fragments arranged by size:
1672 571 34
BclI T'GATC_A
Cuts at: 0 437 443 2277
Size: 437 6 1834
Fragments arranged by size:
1834 437 6
BglI GCCn_nnn'nGGC
Cuts at: 0 653 2277
Size: 653 1624
Bme1580I G_kGCm'C
Cuts at: 0 1625 2277
Size: 1625 652
BmgBI CAC'GTC
Cuts at: 0 339 2277
Size: 339 1938
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2141 2277
Size: 2141 136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1038 1306 2207 2277
Size: 1038 268 901 70
Fragments arranged by size:
1038 901 268 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1420 2277
Size: 1420 857
BsaI GGTCTCn'nnnn_
Cuts at: 0 1194 2277
Size: 1194 1083
BsaAI yAC'GTr
Cuts at: 0 185 410 1122 1909 2271 2277
Size: 185 225 712 787 362 6
Fragments arranged by size:
787 712 362 225 185 6
BsaBI GATnn'nnATC
Cuts at: 0 442 1054 1309 2277
Size: 442 612 255 968
Fragments arranged by size:
968 612 442 255
BsaWI w'CCGG_w
Cuts at: 0 629 2277
Size: 629 1648
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 277 307 803 833 1279 1309 1778 1808
Size: 277 30 496 30 446 30 469 30
Cuts at: 1808 2277
Size: 469
Fragments arranged by size:
496 469 469 446 277 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 624 1294 1786 1846 2277
Size: 265 359 670 492 60 431
Fragments arranged by size:
670 492 431 359 265 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 238 1472 2277
Size: 181 57 1234 805
Fragments arranged by size:
1234 805 181 57
BsiEI CG_ry'CG
Cuts at: 0 1052 2277
Size: 1052 1225
BsiHKAI G_wGCw'C
Cuts at: 0 257 1625 1796 2277
Size: 257 1368 171 481
Fragments arranged by size:
1368 481 257 171
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1177 2277
Size: 1177 1100
Bsp1286I G_dGCh'C
Cuts at: 0 257 920 1625 1796 2277
Size: 257 663 705 171 481
Fragments arranged by size:
705 663 481 257 171
BspHI T'CATG_A
Cuts at: 0 440 1307 2277
Size: 440 867 970
Fragments arranged by size:
970 867 440
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 663 677 2277
Size: 663 14 1600
Fragments arranged by size:
1600 663 14
BsrBI CCG'CTC
Cuts at: 0 614 1152 1290 2277
Size: 614 538 138 987
Fragments arranged by size:
987 614 538 138
BssSI C'ACGA_G
Cuts at: 0 40 199 2277
Size: 40 159 2078
Fragments arranged by size:
2078 159 40
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1005 1533 2277
Size: 1005 528 744
Fragments arranged by size:
1005 744 528
BstBI TT'CG_AA
Cuts at: 0 1501 2277
Size: 1501 776
BstXI CCAn_nnnn'nTGG
Cuts at: 0 126 1470 2277
Size: 126 1344 807
Fragments arranged by size:
1344 807 126
BstYI r'GATC_y
Cuts at: 0 350 2277
Size: 350 1927
BtgI C'CryG_G
Cuts at: 0 16 71 623 1329 1613 1847 2277
Size: 16 55 552 706 284 234 430
Fragments arranged by size:
706 552 430 284 234 55 16
BtsI GCAGTG_nn'
Cuts at: 0 423 2277
Size: 423 1854
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1217 1252 2277
Size: 1217 35 1025
Fragments arranged by size:
1217 1025 35
DraI TTT'AAA
Cuts at: 0 395 1733 1746 2277
Size: 395 1338 13 531
Fragments arranged by size:
1338 531 395 13
DraIII CAC_nnn'GTG
Cuts at: 0 2271 2277
Size: 2271 6
EaeI y'GGCC_r
Cuts at: 0 80 1597 1844 2277
Size: 80 1517 247 433
Fragments arranged by size:
1517 433 247 80
EarI CTCTTCn'nnn_
Cuts at: 0 704 800 1783 2277
Size: 704 96 983 494
Fragments arranged by size:
983 704 494 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 204 2277
Size: 19 185 2073
Fragments arranged by size:
2073 185 19
EcoICRI GAG'CTC
Cuts at: 0 255 2277
Size: 255 2022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1038 1306 1633 1684 1806 2207 2277
Size: 1038 268 327 51 122 401 70
Fragments arranged by size:
1038 401 327 268 122 70 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 234 1168 2277
Size: 234 934 1109
Fragments arranged by size:
1109 934 234
EcoO109I rG'GnC_Cy
Cuts at: 0 12 1555 2277
Size: 12 1543 722
Fragments arranged by size:
1543 722 12
EcoRI G'AATT_C
Cuts at: 0 1762 2277
Size: 1762 515
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 45 1194 2277
Size: 45 1149 1083
Fragments arranged by size:
1149 1083 45
FspI TGC'GCA
Cuts at: 0 1873 2277
Size: 1873 404
HaeII r_GCGC'y
Cuts at: 0 40 1006 2277
Size: 40 966 1271
Fragments arranged by size:
1271 966 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 422 454 1908 1940 2115 2147 2277
Size: 422 32 1454 32 175 32 130
Fragments arranged by size:
1454 422 175 130 32 32 32
HincII GTy'rAC
Cuts at: 0 1161 2277
Size: 1161 1116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1076 1277 2277
Size: 1076 201 1000
Fragments arranged by size:
1076 1000 201
MscI TGG'CCA
Cuts at: 0 1599 1846 2277
Size: 1599 247 431
Fragments arranged by size:
1599 431 247
MslI CAynn'nnrTG
Cuts at: 0 478 1612 1618 2277
Size: 478 1134 6 659
Fragments arranged by size:
1134 659 478 6
MspA1I CmG'CkG
Cuts at: 0 18 73 972 2166 2277
Size: 18 55 899 1194 111
Fragments arranged by size:
1194 899 111 55 18
NcoI C'CATG_G
Cuts at: 0 1613 2277
Size: 1613 664
NdeI CA'TA_TG
Cuts at: 0 1532 2277
Size: 1532 745
NspI r_CATG'y
Cuts at: 0 660 1237 1482 1726 2277
Size: 660 577 245 244 551
Fragments arranged by size:
660 577 551 245 244
PflMI CCAn_nnn'nTGG
Cuts at: 0 267 930 1788 2271 2277
Size: 267 663 858 483 6
Fragments arranged by size:
858 663 483 267 6
PmlI CAC'GTG
Cuts at: 0 185 410 1122 1909 2277
Size: 185 225 712 787 368
Fragments arranged by size:
787 712 368 225 185
PsiI TTA'TAA
Cuts at: 0 1358 2277
Size: 1358 919
PspXI vC'TCGA_Gb
Cuts at: 0 247 2277
Size: 247 2030
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 958 990 2277
Size: 958 32 1287
Fragments arranged by size:
1287 958 32
PstI C_TGCA'G
Cuts at: 0 430 488 674 911 1013 1218 1886 2277
Size: 430 58 186 237 102 205 668 391
Fragments arranged by size:
668 430 391 237 205 186 102 58
PvuI CG_AT'CG
Cuts at: 0 1052 2277
Size: 1052 1225
PvuII CAG'CTG
Cuts at: 0 972 2166 2277
Size: 972 1194 111
Fragments arranged by size:
1194 972 111
SacI G_AGCT'C
Cuts at: 0 257 2277
Size: 257 2020
SacII CC_GC'GG
Cuts at: 0 19 74 2277
Size: 19 55 2203
Fragments arranged by size:
2203 55 19
SbfI CC_TGCA'GG
Cuts at: 0 674 2277
Size: 674 1603
SfcI C'TryA_G
Cuts at: 0 426 484 670 907 1009 1214 1559 1882
Size: 426 58 186 237 102 205 345 323
Cuts at: 1882 2277
Size: 395
Fragments arranged by size:
426 395 345 323 237 205 186 102
58
SmlI C'TyrA_G
Cuts at: 0 247 1435 2277
Size: 247 1188 842
Fragments arranged by size:
1188 842 247
SphI G_CATG'C
Cuts at: 0 660 2277
Size: 660 1617
StuI AGG'CCT
Cuts at: 0 325 2277
Size: 325 1952
StyI C'CwwG_G
Cuts at: 0 1613 2277
Size: 1613 664
TatI w'GTAC_w
Cuts at: 0 1025 2277
Size: 1025 1252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 277 309 1778 1810 1925 1957 2277
Size: 277 32 1469 32 115 32 320
Fragments arranged by size:
1469 320 277 115 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 337 1081 2277
Size: 337 744 1196
Fragments arranged by size:
1196 744 337
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 513 2277
Size: 513 1764
XhoI C'TCGA_G
Cuts at: 0 247 2277
Size: 247 2030
XmnI GAAnn'nnTTC
Cuts at: 0 1762 2277
Size: 1762 515
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlfI AloI AlwNI
ApaLI ApoI AvaI BaeI BanII BbsI BcgI BclI
BglI Bme1580I BmgBI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BssSI BstAPI BstBI BstXI
BstYI BtgI BtsI CspCI DraI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI
NdeI NspI PflMI PmlI PsiI PspXI PsrI PstI
PvuI PvuII SacI SacII SbfI SfcI SmlI SphI
StuI StyI TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI ApaI
AscI AseI AsiSI AvrII BamHI BanI BbeI BbvCI
BciVI BfrBI BglII BlpI BmrI BmtI Bpu10I BsaHI
BseYI BsiWI BsmI BspEI BsrDI BsrFI BsrGI BssHII
BstEII BstZ17I Bsu36I BtgZI ClaI DrdI EagI EcoRV
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PpiI PpuMI PshAI PspOMI
RsrII SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TaqII XbaI XmaI ZraI