Restriction map for
pFN21AE2083
(Linear) (Six-base) MAPSORT of: KIEE2083.seq Check: 2849 from: 1 to: 2271
KIEE2083.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1488 2271
Size: 1488 783
AatII G_ACGT'C
Cuts at: 0 1603 2271
Size: 1603 668
AccI GT'mk_AC
Cuts at: 0 1658 1829 2271
Size: 1658 171 442
Fragments arranged by size:
1658 442 171
Acc65I G'GTAC_C
Cuts at: 0 2102 2271
Size: 2102 169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 597 1036 1044 2271
Size: 589 8 439 8 1227
Fragments arranged by size:
1227 589 439 8 8
AfeI AGC'GCT
Cuts at: 0 77 1756 2271
Size: 77 1679 515
Fragments arranged by size:
1679 515 77
AgeI A'CCGG_T
Cuts at: 0 101 2271
Size: 101 2170
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1069 1101 2271
Size: 1069 32 1170
Fragments arranged by size:
1170 1069 32
AlwNI CAG_nnn'CTG
Cuts at: 0 328 1894 1941 2271
Size: 328 1566 47 330
Fragments arranged by size:
1566 330 328 47
ApaI G_GGCC'C
Cuts at: 0 1381 2271
Size: 1381 890
ApaLI G'TGCA_C
Cuts at: 0 1672 2271
Size: 1672 599
ApoI r'AATT_y
Cuts at: 0 226 301 2271
Size: 226 75 1970
Fragments arranged by size:
1970 226 75
AvaI C'yCGr_G
Cuts at: 0 85 2141 2271
Size: 85 2056 130
Fragments arranged by size:
2056 130 85
BanI G'GyrC_C
Cuts at: 0 233 1788 1876 2102 2271
Size: 233 1555 88 226 169
Fragments arranged by size:
1555 233 226 169 88
BanII G_rGCy'C
Cuts at: 0 1381 1907 2271
Size: 1381 526 364
Fragments arranged by size:
1381 526 364
BbeI G_GCGC'C
Cuts at: 0 1880 2271
Size: 1880 391
BbsI GAAGACnn'nnnn_
Cuts at: 0 55 603 1130 1608 2000 2271
Size: 55 548 527 478 392 271
Fragments arranged by size:
548 527 478 392 271 55
BbvCI CC'TCA_GC
Cuts at: 0 61 321 1540 2271
Size: 61 260 1219 731
Fragments arranged by size:
1219 731 260 61
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1714 1748 2271
Size: 1714 34 523
Fragments arranged by size:
1714 523 34
BclI T'GATC_A
Cuts at: 0 1502 2271
Size: 1502 769
BglI GCCn_nnn'nGGC
Cuts at: 0 2144 2200 2271
Size: 2144 56 71
Fragments arranged by size:
2144 71 56
BglII A'GATC_T
Cuts at: 0 986 1055 2271
Size: 986 69 1216
Fragments arranged by size:
1216 986 69
Bme1580I G_kGCm'C
Cuts at: 0 1381 1676 2271
Size: 1381 295 595
Fragments arranged by size:
1381 595 295
BmrI ACTGGGnnnn_n'
Cuts at: 0 695 2271
Size: 695 1576
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 463 2271
Size: 463 1808
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 511 610 1171 1492 1537 2271
Size: 10 501 99 561 321 45 734
Fragments arranged by size:
734 561 501 321 99 45 10
Bpu10I CC'TnA_GC
Cuts at: 0 61 321 1540 1663 2271
Size: 61 260 1219 123 608
Fragments arranged by size:
1219 608 260 123 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 2271
Size: 697 1574
BsaHI Gr'CG_yC
Cuts at: 0 1600 1877 2271
Size: 1600 277 394
Fragments arranged by size:
1600 394 277
BsaWI w'CCGG_w
Cuts at: 0 101 1329 2222 2271
Size: 101 1228 893 49
Fragments arranged by size:
1228 893 101 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 509 539 1069 1099 2271
Size: 509 30 530 30 1172
Fragments arranged by size:
1172 530 509 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 694 903 1276 1363 1534 1798 1987
Size: 172 522 209 373 87 171 264 189
Cuts at: 1987 2023 2262 2271
Size: 36 239 9
Fragments arranged by size:
522 373 264 239 209 189 172 171
87 36 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 741 1754 2271
Size: 64 677 1013 517
Fragments arranged by size:
1013 677 517 64
BsiEI CG_ry'CG
Cuts at: 0 2241 2271
Size: 2241 30
BsiHKAI G_wGCw'C
Cuts at: 0 500 1676 1690 1907 2271
Size: 500 1176 14 217 364
Fragments arranged by size:
1176 500 364 217 14
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1159 1607 2271
Size: 1159 448 664
Fragments arranged by size:
1159 664 448
Bsp1286I G_dGCh'C
Cuts at: 0 500 1381 1676 1690 1907 2271
Size: 500 881 295 14 217 364
Fragments arranged by size:
881 500 364 295 217 14
BspEI T'CCGG_A
Cuts at: 0 1329 2271
Size: 1329 942
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 290 763 1439 1488 2271
Size: 290 473 676 49 783
Fragments arranged by size:
783 676 473 290 49
BsrBI CCG'CTC
Cuts at: 0 1524 2271
Size: 1524 747
BsrFI r'CCGG_y
Cuts at: 0 101 2105 2131 2271
Size: 101 2004 26 140
Fragments arranged by size:
2004 140 101 26
BstEII G'GTnAC_C
Cuts at: 0 916 2271
Size: 916 1355
BstXI CCAn_nnnn'nTGG
Cuts at: 0 634 2079 2271
Size: 634 1445 192
Fragments arranged by size:
1445 634 192
BstYI r'GATC_y
Cuts at: 0 479 986 1055 2271
Size: 479 507 69 1216
Fragments arranged by size:
1216 507 479 69
Bsu36I CC'TnA_GG
Cuts at: 0 329 1934 2271
Size: 329 1605 337
Fragments arranged by size:
1605 337 329
BtgI C'CryG_G
Cuts at: 0 1115 1367 2148 2231 2271
Size: 1115 252 781 83 40
Fragments arranged by size:
1115 781 252 83 40
BtsI GCAGTG_nn'
Cuts at: 0 520 1742 1792 2054 2132 2271
Size: 520 1222 50 262 78 139
Fragments arranged by size:
1222 520 262 139 78 50
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2108 2143 2271
Size: 2108 35 128
Fragments arranged by size:
2108 128 35
EaeI y'GGCC_r
Cuts at: 0 1118 2129 2234 2271
Size: 1118 1011 105 37
Fragments arranged by size:
1118 1011 105 37
EarI CTCTTCn'nnn_
Cuts at: 0 880 1037 1075 1114 1340 2271
Size: 880 157 38 39 226 931
Fragments arranged by size:
931 880 226 157 39 38
EcoICRI GAG'CTC
Cuts at: 0 1905 2271
Size: 1905 366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 511 589 597 610 1036 1044 1171
Size: 10 501 78 8 13 426 8 127
Cuts at: 1171 1492 1537 2271
Size: 321 45 734
Fragments arranged by size:
734 501 426 321 127 78 45 13
10 8 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 154 1447 1961 2271
Size: 154 1293 514 310
Fragments arranged by size:
1293 514 310 154
EcoO109I rG'GnC_Cy
Cuts at: 0 42 1378 1428 2040 2271
Size: 42 1336 50 612 231
Fragments arranged by size:
1336 612 231 50 42
EcoRV GAT'ATC
Cuts at: 0 783 2271
Size: 783 1488
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 837 1066 2271
Size: 837 229 1205
Fragments arranged by size:
1205 837 229
HaeII r_GCGC'y
Cuts at: 0 79 354 1758 1880 2271
Size: 79 275 1404 122 391
Fragments arranged by size:
1404 391 275 122 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1876 1908 2271
Size: 1876 32 363
Fragments arranged by size:
1876 363 32
HincII GTy'rAC
Cuts at: 0 339 2271
Size: 339 1932
HindIII A'AGCT_T
Cuts at: 0 673 907 2271
Size: 673 234 1364
Fragments arranged by size:
1364 673 234
KasI G'GCGC_C
Cuts at: 0 1876 2271
Size: 1876 395
KpnI G_GTAC'C
Cuts at: 0 2106 2271
Size: 2106 165
MslI CAynn'nnrTG
Cuts at: 0 362 2271
Size: 362 1909
MspA1I CmG'CkG
Cuts at: 0 1016 1369 1548 1749 2271
Size: 1016 353 179 201 522
Fragments arranged by size:
1016 522 353 201 179
NaeI GCC'GGC
Cuts at: 0 2133 2271
Size: 2133 138
NarI GG'CG_CC
Cuts at: 0 1877 2271
Size: 1877 394
NcoI C'CATG_G
Cuts at: 0 2148 2231 2271
Size: 2148 83 40
Fragments arranged by size:
2148 83 40
NgoMIV G'CCGG_C
Cuts at: 0 2131 2271
Size: 2131 140
NspI r_CATG'y
Cuts at: 0 345 390 711 2271
Size: 345 45 321 1560
Fragments arranged by size:
1560 345 321 45
PflMI CCAn_nnn'nTGG
Cuts at: 0 767 1444 2271
Size: 767 677 827
Fragments arranged by size:
827 767 677
PfoI T'CCnGG_A
Cuts at: 0 482 716 943 1091 1514 2271
Size: 482 234 227 148 423 757
Fragments arranged by size:
757 482 423 234 227 148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1069 1101 2271
Size: 1069 32 1170
Fragments arranged by size:
1170 1069 32
PpuMI rG'GwC_Cy
Cuts at: 0 42 1428 2040 2271
Size: 42 1386 612 231
Fragments arranged by size:
1386 612 231 42
PspOMI G'GGCC_C
Cuts at: 0 1377 2271
Size: 1377 894
PstI C_TGCA'G
Cuts at: 0 1076 1142 1799 2049 2271
Size: 1076 66 657 250 222
Fragments arranged by size:
1076 657 250 222 66
PvuII CAG'CTG
Cuts at: 0 1016 1548 2271
Size: 1016 532 723
Fragments arranged by size:
1016 723 532
SacI G_AGCT'C
Cuts at: 0 1907 2271
Size: 1907 364
SacII CC_GC'GG
Cuts at: 0 1370 2271
Size: 1370 901
ScaI AGT'ACT
Cuts at: 0 1477 2271
Size: 1477 794
SexAI A'CCwGG_T
Cuts at: 0 1868 2271
Size: 1868 403
SfcI C'TryA_G
Cuts at: 0 259 570 1072 1138 1795 2045 2271
Size: 259 311 502 66 657 250 226
Fragments arranged by size:
657 502 311 259 250 226 66
SfoI GGC'GCC
Cuts at: 0 1878 2271
Size: 1878 393
SmlI C'TyrA_G
Cuts at: 0 676 2271
Size: 676 1595
StuI AGG'CCT
Cuts at: 0 1147 2271
Size: 1147 1124
StyI C'CwwG_G
Cuts at: 0 1443 2148 2231 2271
Size: 1443 705 83 40
Fragments arranged by size:
1443 705 83 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 49 2271
Size: 49 2222
TatI w'GTAC_w
Cuts at: 0 1475 1766 2271
Size: 1475 291 505
Fragments arranged by size:
1475 505 291
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 388 406 420 438 509 541 1336 1368
Size: 388 18 14 18 71 32 795 32
Cuts at: 1368 2271
Size: 903
Fragments arranged by size:
903 795 388 71 32 32 18 18
14
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1392 2271
Size: 1392 879
XmnI GAAnn'nnTTC
Cuts at: 0 1084 2271
Size: 1084 1187
ZraI GAC'GTC
Cuts at: 0 1601 2271
Size: 1601 670
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AgeI AloI
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BclI BglI BglII Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrFI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
HaeII Hin4I HincII HindIII KasI KpnI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PflMI PfoI PpiI
PpuMI PspOMI PstI PvuII SacI SacII ScaI SexAI
SfcI SfoI SmlI StuI StyI TaqII TatI TstI
XcmI XmnI ZraI
Enzymes that do not cut:
AclI AflII AflIII AhdI AleI AlfI AscI AseI
AsiSI AvrII BaeI BamHI BciVI BfrBI BlpI BmgBI
BmtI BsaI BsaAI BsaBI BseYI BsiWI BsmI BspHI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI
ClaI DraI DraIII DrdI EagI EciI EcoRI FseI
FspI FspAI HpaI MfeI MluI MmeI MscI NdeI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SanDI SapI SbfI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI