Restriction map for
pFN21AE2080
(Linear) (Six-base) MAPSORT of: KIEE2080.seq Check: 4316 from: 1 to: 2259
KIEE2080.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AclI AA'CG_TT
Cuts at: 0 327 2259
Size: 327 1932
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2168 2259
Size: 2168 91
AfeI AGC'GCT
Cuts at: 0 1101 2259
Size: 1101 1158
AflIII A'CryG_T
Cuts at: 0 777 1718 1820 2259
Size: 777 941 102 439
Fragments arranged by size:
941 777 439 102
AhdI GACnn_n'nnGTC
Cuts at: 0 1659 2259
Size: 1659 600
AleI CACnn'nnGTG
Cuts at: 0 1921 2259
Size: 1921 338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 47 733 2259
Size: 47 686 1526
Fragments arranged by size:
1526 686 47
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2113 2145 2259
Size: 2113 32 114
Fragments arranged by size:
2113 114 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1227 1370 2259
Size: 1227 143 889
Fragments arranged by size:
1227 889 143
ApaI G_GGCC'C
Cuts at: 0 127 808 2259
Size: 127 681 1451
Fragments arranged by size:
1451 681 127
ApaLI G'TGCA_C
Cuts at: 0 27 59 2259
Size: 27 32 2200
Fragments arranged by size:
2200 32 27
ApoI r'AATT_y
Cuts at: 0 1463 1730 2259
Size: 1463 267 529
Fragments arranged by size:
1463 529 267
AvaI C'yCGr_G
Cuts at: 0 118 2259
Size: 118 2141
BamHI G'GATC_C
Cuts at: 0 1872 2259
Size: 1872 387
BanI G'GyrC_C
Cuts at: 0 1138 2259
Size: 1138 1121
BanII G_rGCy'C
Cuts at: 0 127 808 1427 1434 1986 2259
Size: 127 681 619 7 552 273
Fragments arranged by size:
681 619 552 273 127 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 2156 2259
Size: 2156 103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 18 52 2259
Size: 18 34 2207
Fragments arranged by size:
2207 34 18
BciVI GTATCCnnnnn_n'
Cuts at: 0 1617 2259
Size: 1617 642
BglI GCCn_nnn'nGGC
Cuts at: 0 843 856 2259
Size: 843 13 1403
Fragments arranged by size:
1403 843 13
Bme1580I G_kGCm'C
Cuts at: 0 31 63 127 808 2259
Size: 31 32 64 681 1451
Fragments arranged by size:
1451 681 64 32 31
BmgBI CAC'GTC
Cuts at: 0 1823 2259
Size: 1823 436
BmtI G_CTAG'C
Cuts at: 0 557 850 2259
Size: 557 293 1409
Fragments arranged by size:
1409 557 293
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1056 2259
Size: 1056 1203
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 1392 1531 2259
Size: 36 1356 139 728
Fragments arranged by size:
1356 728 139 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 239 1550 2259
Size: 239 1311 709
Fragments arranged by size:
1311 709 239
BsaAI yAC'GTr
Cuts at: 0 780 2259
Size: 780 1479
BsaBI GATnn'nnATC
Cuts at: 0 278 2259
Size: 278 1981
BsaHI Gr'CG_yC
Cuts at: 0 933 2259
Size: 933 1326
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 970 1000 2259
Size: 970 30 1259
Fragments arranged by size:
1259 970 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 1032 1389 1568 1897 1990 2259
Size: 166 866 357 179 329 93 269
Fragments arranged by size:
866 357 329 269 179 166 93
BseYI C'CCAG_C
Cuts at: 0 156 800 1004 1936 2132 2259
Size: 156 644 204 932 196 127
Fragments arranged by size:
932 644 204 196 156 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 1093 1104 1136 2259
Size: 656 437 11 32 1123
Fragments arranged by size:
1123 656 437 32 11
BsiEI CG_ry'CG
Cuts at: 0 2217 2259
Size: 2217 42
BsiHKAI G_wGCw'C
Cuts at: 0 31 63 1042 1604 2259
Size: 31 32 979 562 655
Fragments arranged by size:
979 655 562 32 31
BsmI GAATG_Cn'
Cuts at: 0 722 2259
Size: 722 1537
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1120 2204 2259
Size: 1120 1084 55
Fragments arranged by size:
1120 1084 55
Bsp1286I G_dGCh'C
Cuts at: 0 31 63 127 808 1042 1427 1434 1604
Size: 31 32 64 681 234 385 7 170
Cuts at: 1604 1986 2259
Size: 382 273
Fragments arranged by size:
681 385 382 273 234 170 64 32
31 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1115 2259
Size: 1115 1144
BsrDI GCAATG_nn'
Cuts at: 0 391 2259
Size: 391 1868
BsrFI r'CCGG_y
Cuts at: 0 842 2216 2259
Size: 842 1374 43
Fragments arranged by size:
1374 842 43
BsrGI T'GTAC_A
Cuts at: 0 1518 1817 2259
Size: 1518 299 442
Fragments arranged by size:
1518 442 299
BssSI C'ACGA_G
Cuts at: 0 1095 1811 2259
Size: 1095 716 448
Fragments arranged by size:
1095 716 448
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1344 2259
Size: 1344 915
BstYI r'GATC_y
Cuts at: 0 113 1872 2259
Size: 113 1759 387
Fragments arranged by size:
1759 387 113
Bsu36I CC'TnA_GG
Cuts at: 0 1907 2259
Size: 1907 352
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 129 2259
Size: 129 2130
BtsI GCAGTG_nn'
Cuts at: 0 29 31 1002 2259
Size: 29 2 971 1257
Fragments arranged by size:
1257 971 29 2
DrdI GACnn_nn'nnGTC
Cuts at: 0 1564 2259
Size: 1564 695
EaeI y'GGCC_r
Cuts at: 0 140 840 1052 1259 2259
Size: 140 700 212 207 1000
Fragments arranged by size:
1000 700 212 207 140
EarI CTCTTCn'nnn_
Cuts at: 0 3 364 1545 1788 1845 2259
Size: 3 361 1181 243 57 414
Fragments arranged by size:
1181 414 361 243 57 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 1392 1531 2168 2259
Size: 36 1356 139 637 91
Fragments arranged by size:
1356 637 139 91 36
EcoO109I rG'GnC_Cy
Cuts at: 0 123 804 805 835 1367 1778 2259
Size: 123 681 1 30 532 411 481
Fragments arranged by size:
681 532 481 411 123 30 1
EcoRI G'AATT_C
Cuts at: 0 1730 2259
Size: 1730 529
HaeII r_GCGC'y
Cuts at: 0 1103 2259
Size: 1103 1156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 470 502 1439 1471 1645 1677 1770 1802
Size: 470 32 937 32 174 32 93 32
Cuts at: 1802 1857 1889 2259
Size: 55 32 370
Fragments arranged by size:
937 470 370 174 93 55 32 32
32 32 32
MfeI C'AATT_G
Cuts at: 0 758 1535 2259
Size: 758 777 724
Fragments arranged by size:
777 758 724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 660 684 1492 1952 2259
Size: 660 24 808 460 307
Fragments arranged by size:
808 660 460 307 24
MscI TGG'CCA
Cuts at: 0 1054 1261 2259
Size: 1054 207 998
Fragments arranged by size:
1054 998 207
MslI CAynn'nnrTG
Cuts at: 0 715 1921 2259
Size: 715 1206 338
Fragments arranged by size:
1206 715 338
MspA1I CmG'CkG
Cuts at: 0 109 1070 1257 2259
Size: 109 961 187 1002
Fragments arranged by size:
1002 961 187 109
NaeI GCC'GGC
Cuts at: 0 844 2259
Size: 844 1415
NdeI CA'TA_TG
Cuts at: 0 1799 2259
Size: 1799 460
NgoMIV G'CCGG_C
Cuts at: 0 842 2259
Size: 842 1417
NheI G'CTAG_C
Cuts at: 0 553 846 2259
Size: 553 293 1413
Fragments arranged by size:
1413 553 293
NspI r_CATG'y
Cuts at: 0 1519 1722 2259
Size: 1519 203 537
Fragments arranged by size:
1519 537 203
PciI A'CATG_T
Cuts at: 0 1718 2259
Size: 1718 541
PflMI CCAn_nnn'nTGG
Cuts at: 0 1370 2259
Size: 1370 889
PfoI T'CCnGG_A
Cuts at: 0 771 2259
Size: 771 1488
PmlI CAC'GTG
Cuts at: 0 780 2259
Size: 780 1479
PpuMI rG'GwC_Cy
Cuts at: 0 1367 1778 2259
Size: 1367 411 481
Fragments arranged by size:
1367 481 411
PsiI TTA'TAA
Cuts at: 0 2186 2259
Size: 2186 73
PspOMI G'GGCC_C
Cuts at: 0 123 804 2259
Size: 123 681 1455
Fragments arranged by size:
1455 681 123
PstI C_TGCA'G
Cuts at: 0 26 2259
Size: 26 2233
SapI GCTCTTCn'nnn_
Cuts at: 0 3 2259
Size: 3 2256
SexAI A'CCwGG_T
Cuts at: 0 1226 2259
Size: 1226 1033
SfcI C'TryA_G
Cuts at: 0 22 1688 2259
Size: 22 1666 571
Fragments arranged by size:
1666 571 22
SmaI CCC'GGG
Cuts at: 0 120 2259
Size: 120 2139
SspI AAT'ATT
Cuts at: 0 1543 2259
Size: 1543 716
StuI AGG'CCT
Cuts at: 0 1013 2259
Size: 1013 1246
TatI w'GTAC_w
Cuts at: 0 1518 1817 2259
Size: 1518 299 442
Fragments arranged by size:
1518 442 299
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 618 650 1736 1768 2259
Size: 618 32 1086 32 491
Fragments arranged by size:
1086 618 491 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1205 1344 2259
Size: 1205 139 915
Fragments arranged by size:
1205 915 139
XmaI C'CCGG_G
Cuts at: 0 118 2259
Size: 118 2141
Enzymes that do cut:
AclI AcuI AfeI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbsI BcgI BciVI BglI Bme1580I BmgBI BmtI BplI
BpmI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI
BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgZI BtsI
DrdI EaeI EarI Eco57MI EcoO109I EcoRI HaeII Hin4I
MfeI MmeI MscI MslI MspA1I NaeI NdeI NgoMIV
NheI NspI PciI PflMI PfoI PmlI PpuMI PsiI
PspOMI PstI SapI SexAI SfcI SmaI SspI StuI
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AgeI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BclI BfrBI BglII
BlpI BmrI Bpu10I BpuEI BsaWI BsiWI BspEI BspHI
BsrBI BssHII BstAPI BstBI BstEII BstZ17I BtgI ClaI
CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MluI NarI NcoI NotI NruI NsiI PacI
PasI PmeI PpiI PshAI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
StyI SwaI TaqII Tth111I XbaI XhoI XmnI ZraI