Restriction map for
pFN21AE2070
(Linear) (Six-base) MAPSORT of: KIEE2070.seq Check: 3965 from: 1 to: 2184
KIEE2070.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1300 2184
Size: 1300 884
AccI GT'mk_AC
Cuts at: 0 640 1094 1553 2099 2184
Size: 640 454 459 546 85
Fragments arranged by size:
640 546 459 454 85
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 1027 1069 1757 2087 2184
Size: 35 992 42 688 330 97
Fragments arranged by size:
992 688 330 97 42 35
AfeI AGC'GCT
Cuts at: 0 2029 2184
Size: 2029 155
AflIII A'CryG_T
Cuts at: 0 1937 1973 2184
Size: 1937 36 211
Fragments arranged by size:
1937 211 36
AleI CACnn'nnGTG
Cuts at: 0 1519 2184
Size: 1519 665
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1924 1956 2184
Size: 1924 32 228
Fragments arranged by size:
1924 228 32
AlwNI CAG_nnn'CTG
Cuts at: 0 258 539 1395 2184
Size: 258 281 856 789
Fragments arranged by size:
856 789 281 258
ApaI G_GGCC'C
Cuts at: 0 1455 2184
Size: 1455 729
AvaI C'yCGr_G
Cuts at: 0 849 1610 1911 2184
Size: 849 761 301 273
Fragments arranged by size:
849 761 301 273
BamHI G'GATC_C
Cuts at: 0 1472 2184
Size: 1472 712
BanI G'GyrC_C
Cuts at: 0 717 1872 2184
Size: 717 1155 312
Fragments arranged by size:
1155 717 312
BanII G_rGCy'C
Cuts at: 0 628 941 1107 1199 1373 1455 2171 2184
Size: 628 313 166 92 174 82 716 13
Fragments arranged by size:
716 628 313 174 166 92 82 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 533 567 2184
Size: 533 34 1617
Fragments arranged by size:
1617 533 34
BclI T'GATC_A
Cuts at: 0 587 1130 2184
Size: 587 543 1054
Fragments arranged by size:
1054 587 543
BglI GCCn_nnn'nGGC
Cuts at: 0 509 2184
Size: 509 1675
BlpI GC'TnA_GC
Cuts at: 0 1367 1602 2184
Size: 1367 235 582
Fragments arranged by size:
1367 582 235
Bme1580I G_kGCm'C
Cuts at: 0 722 904 1455 1691 2184
Size: 722 182 551 236 493
Fragments arranged by size:
722 551 493 236 182
BmgBI CAC'GTC
Cuts at: 0 1061 2184
Size: 1061 1123
BmrI ACTGGGnnnn_n'
Cuts at: 0 116 590 2136 2184
Size: 116 474 1546 48
Fragments arranged by size:
1546 474 116 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1636 1926 2184
Size: 1636 290 258
Fragments arranged by size:
1636 290 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 888 2184
Size: 673 215 1296
Fragments arranged by size:
1296 673 215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1750 2040 2184
Size: 1750 290 144
Fragments arranged by size:
1750 290 144
BsaI GGTCTCn'nnnn_
Cuts at: 0 274 2115 2184
Size: 274 1841 69
Fragments arranged by size:
1841 274 69
BsaAI yAC'GTr
Cuts at: 0 1976 2121 2184
Size: 1976 145 63
Fragments arranged by size:
1976 145 63
BsaWI w'CCGG_w
Cuts at: 0 353 2184
Size: 353 1831
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 481 511 1924 1954 2184
Size: 481 30 1413 30 230
Fragments arranged by size:
1413 481 230 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 388 460 670 2184
Size: 388 72 210 1514
Fragments arranged by size:
1514 388 210 72
BseYI C'CCAG_C
Cuts at: 0 1502 1758 1775 2030 2184
Size: 1502 256 17 255 154
Fragments arranged by size:
1502 256 255 154 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 1690 2184
Size: 573 1117 494
Fragments arranged by size:
1117 573 494
BsiHKAI G_wGCw'C
Cuts at: 0 628 977 1622 2184
Size: 628 349 645 562
Fragments arranged by size:
645 628 562 349
BsmI GAATG_Cn'
Cuts at: 0 637 2077 2184
Size: 637 1440 107
Fragments arranged by size:
1440 637 107
Bsp1286I G_dGCh'C
Cuts at: 0 628 722 904 941 977 1107 1199 1373
Size: 628 94 182 37 36 130 92 174
Cuts at: 1373 1455 1622 1691 2171 2184
Size: 82 167 69 480 13
Fragments arranged by size:
628 480 182 174 167 130 94 92
82 69 37 36 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 547 683 1300 2076 2184
Size: 547 136 617 776 108
Fragments arranged by size:
776 617 547 136 108
BsrBI CCG'CTC
Cuts at: 0 1322 1910 2184
Size: 1322 588 274
Fragments arranged by size:
1322 588 274
BsrFI r'CCGG_y
Cuts at: 0 1771 2184
Size: 1771 413
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1572 2184
Size: 1572 612
BstXI CCAn_nnnn'nTGG
Cuts at: 0 100 651 2121 2184
Size: 100 551 1470 63
Fragments arranged by size:
1470 551 100 63
BstYI r'GATC_y
Cuts at: 0 89 1472 2184
Size: 89 1383 712
Fragments arranged by size:
1383 712 89
BstZ17I GTA'TAC
Cuts at: 0 641 2184
Size: 641 1543
Bsu36I CC'TnA_GG
Cuts at: 0 713 2184
Size: 713 1471
BtgI C'CryG_G
Cuts at: 0 62 924 1384 1517 1868 2184
Size: 62 862 460 133 351 316
Fragments arranged by size:
862 460 351 316 133 62
BtsI GCAGTG_nn'
Cuts at: 0 561 1827 2184
Size: 561 1266 357
Fragments arranged by size:
1266 561 357
DraIII CAC_nnn'GTG
Cuts at: 0 63 2158 2184
Size: 63 2095 26
Fragments arranged by size:
2095 63 26
EaeI y'GGCC_r
Cuts at: 0 84 306 1769 2184
Size: 84 222 1463 415
Fragments arranged by size:
1463 415 222 84
EarI CTCTTCn'nnn_
Cuts at: 0 571 1384 2184
Size: 571 813 800
Fragments arranged by size:
813 800 571
EcoICRI GAG'CTC
Cuts at: 0 626 2184
Size: 626 1558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 673 888 1027 1069 1757 2087 2184
Size: 35 638 215 139 42 688 330 97
Fragments arranged by size:
688 638 330 215 139 97 42 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 43 2184
Size: 43 2141
EcoO109I rG'GnC_Cy
Cuts at: 0 536 769 1392 1451 1452 1696 1997 2062
Size: 536 233 623 59 1 244 301 65
Cuts at: 2062 2184
Size: 122
Fragments arranged by size:
623 536 301 244 233 122 65 59
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 198 975 2184
Size: 198 777 1209
Fragments arranged by size:
1209 777 198
HaeII r_GCGC'y
Cuts at: 0 2031 2184
Size: 2031 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 436 468 1029 1061 2184
Size: 436 32 561 32 1123
Fragments arranged by size:
1123 561 436 32 32
HincII GTy'rAC
Cuts at: 0 672 1596 2184
Size: 672 924 588
Fragments arranged by size:
924 672 588
HindIII A'AGCT_T
Cuts at: 0 175 2184
Size: 175 2009
MscI TGG'CCA
Cuts at: 0 86 2184
Size: 86 2098
MslI CAynn'nnrTG
Cuts at: 0 409 1519 2184
Size: 409 1110 665
Fragments arranged by size:
1110 665 409
MspA1I CmG'CkG
Cuts at: 0 983 1606 1674 2184
Size: 983 623 68 510
Fragments arranged by size:
983 623 510 68
NaeI GCC'GGC
Cuts at: 0 1773 2184
Size: 1773 411
NcoI C'CATG_G
Cuts at: 0 924 2184
Size: 924 1260
NgoMIV G'CCGG_C
Cuts at: 0 1771 2184
Size: 1771 413
NspI r_CATG'y
Cuts at: 0 1546 1758 1941 2184
Size: 1546 212 183 243
Fragments arranged by size:
1546 243 212 183
PasI CC'CwG_GG
Cuts at: 0 1692 2184
Size: 1692 492
PciI A'CATG_T
Cuts at: 0 1937 2184
Size: 1937 247
PflMI CCAn_nnn'nTGG
Cuts at: 0 63 258 315 405 1306 1758 2184
Size: 63 195 57 90 901 452 426
Fragments arranged by size:
901 452 426 195 90 63 57
PfoI T'CCnGG_A
Cuts at: 0 1468 2184
Size: 1468 716
PmlI CAC'GTG
Cuts at: 0 1976 2184
Size: 1976 208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1924 1956 2184
Size: 1924 32 228
Fragments arranged by size:
1924 228 32
PpuMI rG'GwC_Cy
Cuts at: 0 536 1696 2062 2184
Size: 536 1160 366 122
Fragments arranged by size:
1160 536 366 122
PspOMI G'GGCC_C
Cuts at: 0 1451 2184
Size: 1451 733
PstI C_TGCA'G
Cuts at: 0 680 891 1679 1853 2184
Size: 680 211 788 174 331
Fragments arranged by size:
788 680 331 211 174
PvuII CAG'CTG
Cuts at: 0 983 1606 1674 2184
Size: 983 623 68 510
Fragments arranged by size:
983 623 510 68
SacI G_AGCT'C
Cuts at: 0 628 2184
Size: 628 1556
SanDI GG'GwC_CC
Cuts at: 0 1696 2184
Size: 1696 488
SexAI A'CCwGG_T
Cuts at: 0 251 2184
Size: 251 1933
SfcI C'TryA_G
Cuts at: 0 676 887 910 1098 1675 1849 2184
Size: 676 211 23 188 577 174 335
Fragments arranged by size:
676 577 335 211 188 174 23
SmlI C'TyrA_G
Cuts at: 0 1729 2019 2184
Size: 1729 290 165
Fragments arranged by size:
1729 290 165
StuI AGG'CCT
Cuts at: 0 2045 2184
Size: 2045 139
StyI C'CwwG_G
Cuts at: 0 924 1248 1535 1723 1980 2184
Size: 924 324 287 188 257 204
Fragments arranged by size:
924 324 287 257 204 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 823 1640 2022 2118 2184
Size: 823 817 382 96 66
Fragments arranged by size:
823 817 382 96 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1045 1077 1281 1313 2116 2148 2184
Size: 1045 32 204 32 803 32 36
Fragments arranged by size:
1045 803 204 36 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1836 2184
Size: 1836 348
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 478 1433 1800 2184
Size: 478 955 367 384
Fragments arranged by size:
955 478 384 367
XmnI GAAnn'nnTTC
Cuts at: 0 1939 2184
Size: 1939 245
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AloI AlwNI
ApaI AvaI BamHI BanI BanII BcgI BclI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI BstYI BstZ17I
Bsu36I BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII
SacI SanDI SexAI SfcI SmlI StuI StyI TaqII
TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AlfI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BbvCI BciVI BfrBI BglII BmtI Bpu10I BsaBI BsaHI
BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstBI BstEII BtgZI ClaI CspCI DraI DrdI
EagI EciI EcoRI EcoRV FseI FspI FspAI HpaI
KasI KpnI MfeI MluI MmeI NarI NdeI NheI
NotI NruI NsiI PacI PmeI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TatI XbaI XhoI XmaI ZraI