Restriction map for
pFN21AE2068
(Linear) (Six-base) MAPSORT of: KIEE2068.seq Check: 4692 from: 1 to: 2181
KIEE2068.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1015 2181
Size: 1015 1166
AccI GT'mk_AC
Cuts at: 0 528 2181
Size: 528 1653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 1124 2181
Size: 1064 60 1057
Fragments arranged by size:
1064 1057 60
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1156 2054 2181
Size: 1156 898 127
Fragments arranged by size:
1156 898 127
AlwNI CAG_nnn'CTG
Cuts at: 0 1129 1759 2181
Size: 1129 630 422
Fragments arranged by size:
1129 630 422
ApoI r'AATT_y
Cuts at: 0 1349 2181
Size: 1349 832
BanI G'GyrC_C
Cuts at: 0 685 1230 2149 2181
Size: 685 545 919 32
Fragments arranged by size:
919 685 545 32
BanII G_rGCy'C
Cuts at: 0 1731 1809 2012 2181
Size: 1731 78 203 169
Fragments arranged by size:
1731 203 169 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 488 1104 1859 2181
Size: 488 616 755 322
Fragments arranged by size:
755 616 488 322
BbvCI CC'TCA_GC
Cuts at: 0 2060 2181
Size: 2060 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 163 197 2181
Size: 163 34 1984
Fragments arranged by size:
1984 163 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2 2181
Size: 2 2179
BfrBI ATG'CAT
Cuts at: 0 831 2181
Size: 831 1350
BlpI GC'TnA_GC
Cuts at: 0 1808 2181
Size: 1808 373
Bme1580I G_kGCm'C
Cuts at: 0 690 2154 2181
Size: 690 1464 27
Fragments arranged by size:
1464 690 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 873 2181
Size: 873 1308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 2181
Size: 821 1360
Bpu10I CC'TnA_GC
Cuts at: 0 2060 2181
Size: 2060 121
BsaAI yAC'GTr
Cuts at: 0 826 2181
Size: 826 1355
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1421 2181
Size: 1421 760
BsiEI CG_ry'CG
Cuts at: 0 143 2181
Size: 143 2038
BsiHKAI G_wGCw'C
Cuts at: 0 1809 1875 2012 2181
Size: 1809 66 137 169
Fragments arranged by size:
1809 169 137 66
Bsp1286I G_dGCh'C
Cuts at: 0 690 1731 1809 1875 2012 2154 2181
Size: 690 1041 78 66 137 142 27
Fragments arranged by size:
1041 690 142 137 78 66 27
BspHI T'CATG_A
Cuts at: 0 195 515 2181
Size: 195 320 1666
Fragments arranged by size:
1666 320 195
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1015 2181
Size: 1015 1166
BsrDI GCAATG_nn'
Cuts at: 0 1361 1484 2181
Size: 1361 123 697
Fragments arranged by size:
1361 697 123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 230 1021 1468 2181
Size: 230 791 447 713
Fragments arranged by size:
791 713 447 230
BstBI TT'CG_AA
Cuts at: 0 63 1254 2181
Size: 63 1191 927
Fragments arranged by size:
1191 927 63
BstYI r'GATC_y
Cuts at: 0 176 854 2181
Size: 176 678 1327
Fragments arranged by size:
1327 678 176
BstZ17I GTA'TAC
Cuts at: 0 529 2181
Size: 529 1652
Bsu36I CC'TnA_GG
Cuts at: 0 257 2181
Size: 257 1924
BtsI GCAGTG_nn'
Cuts at: 0 1118 1832 2181
Size: 1118 714 349
Fragments arranged by size:
1118 714 349
EaeI y'GGCC_r
Cuts at: 0 2120 2181
Size: 2120 61
EarI CTCTTCn'nnn_
Cuts at: 0 842 1100 1178 1904 2181
Size: 842 258 78 726 277
Fragments arranged by size:
842 726 277 258 78
EcoICRI GAG'CTC
Cuts at: 0 1807 2010 2181
Size: 1807 203 171
Fragments arranged by size:
1807 203 171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 1064 1124 2181
Size: 821 243 60 1057
Fragments arranged by size:
1057 821 243 60
EcoO109I rG'GnC_Cy
Cuts at: 0 1392 2181
Size: 1392 789
EcoRV GAT'ATC
Cuts at: 0 672 2181
Size: 672 1509
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1087 1306 2181
Size: 1087 219 875
Fragments arranged by size:
1087 875 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 100 132 952 984 1545 1577 1646 1678
Size: 100 32 820 32 561 32 69 32
Cuts at: 1678 2155 2181
Size: 477 26
Fragments arranged by size:
820 561 477 100 69 32 32 32
32 26
HincII GTy'rAC
Cuts at: 0 1791 2181
Size: 1791 390
HindIII A'AGCT_T
Cuts at: 0 152 2181
Size: 152 2029
MfeI C'AATT_G
Cuts at: 0 1019 1169 2181
Size: 1019 150 1012
Fragments arranged by size:
1019 1012 150
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 750 2181
Size: 277 473 1431
Fragments arranged by size:
1431 473 277
MscI TGG'CCA
Cuts at: 0 2122 2181
Size: 2122 59
MslI CAynn'nnrTG
Cuts at: 0 1715 1718 2175 2181
Size: 1715 3 457 6
Fragments arranged by size:
1715 457 6 3
MspA1I CmG'CkG
Cuts at: 0 1474 2181
Size: 1474 707
NdeI CA'TA_TG
Cuts at: 0 1323 1785 1905 2176 2181
Size: 1323 462 120 271 5
Fragments arranged by size:
1323 462 271 120 5
NsiI A_TGCA'T
Cuts at: 0 833 2181
Size: 833 1348
NspI r_CATG'y
Cuts at: 0 174 2181
Size: 174 2007
PpuMI rG'GwC_Cy
Cuts at: 0 1392 2181
Size: 1392 789
PsiI TTA'TAA
Cuts at: 0 1867 2181
Size: 1867 314
PstI C_TGCA'G
Cuts at: 0 226 419 1125 2181
Size: 226 193 706 1056
Fragments arranged by size:
1056 706 226 193
PvuII CAG'CTG
Cuts at: 0 1474 2181
Size: 1474 707
SacI G_AGCT'C
Cuts at: 0 1809 2012 2181
Size: 1809 203 169
Fragments arranged by size:
1809 203 169
SfcI C'TryA_G
Cuts at: 0 222 415 680 1121 2181
Size: 222 193 265 441 1060
Fragments arranged by size:
1060 441 265 222 193
SpeI A'CTAG_T
Cuts at: 0 1507 2181
Size: 1507 674
SphI G_CATG'C
Cuts at: 0 174 2181
Size: 174 2007
SspI AAT'ATT
Cuts at: 0 780 2181
Size: 780 1401
StuI AGG'CCT
Cuts at: 0 25 35 2181
Size: 25 10 2146
Fragments arranged by size:
2146 25 10
StyI C'CwwG_G
Cuts at: 0 724 1581 1625 2181
Size: 724 857 44 556
Fragments arranged by size:
857 724 556 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 386 2181
Size: 386 1795
TatI w'GTAC_w
Cuts at: 0 535 2181
Size: 535 1646
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1494 1526 2181
Size: 1494 32 655
Fragments arranged by size:
1494 655 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 756 1450 2181
Size: 756 694 731
Fragments arranged by size:
756 731 694
XmnI GAAnn'nnTTC
Cuts at: 0 1607 2181
Size: 1607 574
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI ApoI BanI BanII
BbsI BbvCI BcgI BciVI BfrBI BlpI Bme1580I BplI
BpmI Bpu10I BsaAI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrDI BstAPI BstBI BstYI BstZ17I Bsu36I BtsI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI Hin4I
HincII HindIII MfeI MmeI MscI MslI MspA1I NdeI
NsiI NspI PpuMI PsiI PstI PvuII SacI SfcI
SpeI SphI SspI StuI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BclI BglI BglII BmgBI
BmrI BmtI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiWI BsmI BsmBI BspEI BsrBI BsrFI
BsrGI BssHII BssSI BstEII BstXI BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
NaeI NarI NcoI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SrfI SwaI Tth111I XbaI XhoI
XmaI ZraI