Restriction map for
pFN21AE2066
(Linear) (Six-base) MAPSORT of: KIEE2066.seq Check: 6771 from: 1 to: 2175
KIEE2066.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 716 1253 1742 2125 2175
Size: 176 540 537 489 383 50
Fragments arranged by size:
540 537 489 383 176 50
AflII C'TTAA_G
Cuts at: 0 814 1420 2175
Size: 814 606 755
Fragments arranged by size:
814 755 606
AlwNI CAG_nnn'CTG
Cuts at: 0 324 2175
Size: 324 1851
ApaLI G'TGCA_C
Cuts at: 0 50 2175
Size: 50 2125
ApoI r'AATT_y
Cuts at: 0 486 494 780 803 1899 2094 2175
Size: 486 8 286 23 1096 195 81
Fragments arranged by size:
1096 486 286 195 81 23 8
BamHI G'GATC_C
Cuts at: 0 314 2175
Size: 314 1861
BanI G'GyrC_C
Cuts at: 0 15 298 2175
Size: 15 283 1877
Fragments arranged by size:
1877 283 15
BanII G_rGCy'C
Cuts at: 0 1513 2148 2175
Size: 1513 635 27
Fragments arranged by size:
1513 635 27
BbeI G_GCGC'C
Cuts at: 0 19 2175
Size: 19 2156
BbsI GAAGACnn'nnnn_
Cuts at: 0 203 911 1060 1655 2113 2175
Size: 203 708 149 595 458 62
Fragments arranged by size:
708 595 458 203 149 62
BciVI GTATCCnnnnn_n'
Cuts at: 0 117 2175
Size: 117 2058
BglII A'GATC_T
Cuts at: 0 864 1416 1542 2175
Size: 864 552 126 633
Fragments arranged by size:
864 633 552 126
BlpI GC'TnA_GC
Cuts at: 0 560 2175
Size: 560 1615
Bme1580I G_kGCm'C
Cuts at: 0 54 303 2175
Size: 54 249 1872
Fragments arranged by size:
1872 249 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 762 2175
Size: 762 1413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 658 1303 1447 2175
Size: 44 614 645 144 728
Fragments arranged by size:
728 645 614 144 44
Bpu10I CC'TnA_GC
Cuts at: 0 1844 2175
Size: 1844 331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 847 1618 2175
Size: 602 245 771 557
Fragments arranged by size:
771 602 557 245
BsaHI Gr'CG_yC
Cuts at: 0 16 1740 2175
Size: 16 1724 435
Fragments arranged by size:
1724 435 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 844 1735 2175
Size: 844 891 440
Fragments arranged by size:
891 844 440
BseYI C'CCAG_C
Cuts at: 0 1462 2175
Size: 1462 713
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 1859 2175
Size: 350 1509 316
Fragments arranged by size:
1509 350 316
BsiHKAI G_wGCw'C
Cuts at: 0 54 1513 2148 2175
Size: 54 1459 635 27
Fragments arranged by size:
1459 635 54 27
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1747 2175
Size: 1747 428
Bsp1286I G_dGCh'C
Cuts at: 0 54 303 1513 2148 2175
Size: 54 249 1210 635 27
Fragments arranged by size:
1210 635 249 54 27
BspHI T'CATG_A
Cuts at: 0 2079 2175
Size: 2079 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 579 2175
Size: 579 1596
BstAPI GCAn_nnn'nTGC
Cuts at: 0 400 2175
Size: 400 1775
BstEII G'GTnAC_C
Cuts at: 0 475 591 2175
Size: 475 116 1584
Fragments arranged by size:
1584 475 116
BstYI r'GATC_y
Cuts at: 0 314 321 864 1416 1542 2175
Size: 314 7 543 552 126 633
Fragments arranged by size:
633 552 543 314 126 7
Bsu36I CC'TnA_GG
Cuts at: 0 11 2175
Size: 11 2164
DraI TTT'AAA
Cuts at: 0 132 2175
Size: 132 2043
EaeI y'GGCC_r
Cuts at: 0 1454 2175
Size: 1454 721
EarI CTCTTCn'nnn_
Cuts at: 0 605 1205 2175
Size: 605 600 970
Fragments arranged by size:
970 605 600
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 327 2175
Size: 327 1848
EcoICRI GAG'CTC
Cuts at: 0 1511 2146 2175
Size: 1511 635 29
Fragments arranged by size:
1511 635 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 176 658 716 1253 1303 1447 1742
Size: 44 132 482 58 537 50 144 295
Cuts at: 1742 2125 2175
Size: 383 50
Fragments arranged by size:
537 482 383 295 144 132 58 50
50 44
EcoRI G'AATT_C
Cuts at: 0 494 2175
Size: 494 1681
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 36 2175
Size: 36 2139
HaeII r_GCGC'y
Cuts at: 0 19 2175
Size: 19 2156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 306 338 667 699 1056 1088 1401 1420
Size: 306 32 329 32 357 32 313 19
Cuts at: 1420 1433 1452 1500 1532 2175
Size: 13 19 48 32 643
Fragments arranged by size:
643 357 329 313 306 48 32 32
32 32 19 19 13
HincII GTy'rAC
Cuts at: 0 168 915 1397 2175
Size: 168 747 482 778
Fragments arranged by size:
778 747 482 168
HindIII A'AGCT_T
Cuts at: 0 501 1003 2175
Size: 501 502 1172
Fragments arranged by size:
1172 502 501
HpaI GTT'AAC
Cuts at: 0 1397 2175
Size: 1397 778
KasI G'GCGC_C
Cuts at: 0 15 2175
Size: 15 2160
MfeI C'AATT_G
Cuts at: 0 1204 2175
Size: 1204 971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 2175
Size: 317 1858
MslI CAynn'nnrTG
Cuts at: 0 577 2175
Size: 577 1598
MspA1I CmG'CkG
Cuts at: 0 885 1665 1979 2175
Size: 885 780 314 196
Fragments arranged by size:
885 780 314 196
NarI GG'CG_CC
Cuts at: 0 16 2175
Size: 16 2159
NdeI CA'TA_TG
Cuts at: 0 1875 2175
Size: 1875 300
NspI r_CATG'y
Cuts at: 0 1924 2175
Size: 1924 251
PflMI CCAn_nnn'nTGG
Cuts at: 0 324 2175
Size: 324 1851
PfoI T'CCnGG_A
Cuts at: 0 317 2175
Size: 317 1858
PstI C_TGCA'G
Cuts at: 0 590 1550 2175
Size: 590 960 625
Fragments arranged by size:
960 625 590
PvuII CAG'CTG
Cuts at: 0 885 1665 2175
Size: 885 780 510
Fragments arranged by size:
885 780 510
SacI G_AGCT'C
Cuts at: 0 1513 2148 2175
Size: 1513 635 27
Fragments arranged by size:
1513 635 27
SfcI C'TryA_G
Cuts at: 0 286 586 1546 2175
Size: 286 300 960 629
Fragments arranged by size:
960 629 300 286
SfoI GGC'GCC
Cuts at: 0 17 2175
Size: 17 2158
SmlI C'TyrA_G
Cuts at: 0 617 814 826 1420 1633 2175
Size: 617 197 12 594 213 542
Fragments arranged by size:
617 594 542 213 197 12
SspI AAT'ATT
Cuts at: 0 837 2175
Size: 837 1338
StyI C'CwwG_G
Cuts at: 0 1457 2175
Size: 1457 718
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 610 2175
Size: 610 1565
Tth111I GACn'n_nGTC
Cuts at: 0 910 1554 2175
Size: 910 644 621
Fragments arranged by size:
910 644 621
XbaI T'CTAG_A
Cuts at: 0 1225 1539 2175
Size: 1225 314 636
Fragments arranged by size:
1225 636 314
XmnI GAAnn'nnTTC
Cuts at: 0 28 1004 2175
Size: 28 976 1171
Fragments arranged by size:
1171 976 28
Enzymes that do cut:
AcuI AflII AlwNI ApaLI ApoI BamHI BanI BanII
BbeI BbsI BciVI BglII BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaHI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BstAPI BstEII BstYI Bsu36I DraI
EaeI EarI EciI EcoICRI Eco57MI EcoRI FalI HaeII
Hin4I HincII HindIII HpaI KasI MfeI MmeI MslI
MspA1I NarI NdeI NspI PflMI PfoI PstI PvuII
SacI SfcI SfoI SmlI SspI StyI TaqII Tth111I
XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BbvCI BcgI BclI BfrBI BglI
BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI BsaXI
BsiEI BsiWI BsmI BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstXI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EagI EcoNI EcoO109I EcoRV
FseI FspI FspAI KpnI MluI MscI NaeI NcoI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TatI TstI XcmI XhoI
XmaI ZraI