Restriction map for
pFN21AE2059
(Linear) (Six-base) MAPSORT of: KIEE2059.seq Check: 4965 from: 1 to: 2103
KIEE2059.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1202 1726 2103
Size: 1202 524 377
Fragments arranged by size:
1202 524 377
Acc65I G'GTAC_C
Cuts at: 0 794 2103
Size: 794 1309
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 2103
Size: 727 1376
AflIII A'CryG_T
Cuts at: 0 1850 2103
Size: 1850 253
AhdI GACnn_n'nnGTC
Cuts at: 0 42 2103
Size: 42 2061
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 274 284 1069 1900 2103
Size: 274 10 785 831 203
Fragments arranged by size:
831 785 274 203 10
AlwNI CAG_nnn'CTG
Cuts at: 0 111 180 1476 2004 2103
Size: 111 69 1296 528 99
Fragments arranged by size:
1296 528 111 99 69
ApaI G_GGCC'C
Cuts at: 0 647 2103
Size: 647 1456
ApaLI G'TGCA_C
Cuts at: 0 469 2103
Size: 469 1634
ApoI r'AATT_y
Cuts at: 0 722 2103
Size: 722 1381
AvaI C'yCGr_G
Cuts at: 0 1039 1563 2103
Size: 1039 524 540
Fragments arranged by size:
1039 540 524
AvrII C'CTAG_G
Cuts at: 0 1660 2103
Size: 1660 443
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 267 300 2103
Size: 267 33 1803
Fragments arranged by size:
1803 267 33
BamHI G'GATC_C
Cuts at: 0 845 2103
Size: 845 1258
BanI G'GyrC_C
Cuts at: 0 31 794 2103
Size: 31 763 1309
Fragments arranged by size:
1309 763 31
BanII G_rGCy'C
Cuts at: 0 191 647 986 1352 1410 1421 1651 2103
Size: 191 456 339 366 58 11 230 452
Fragments arranged by size:
456 452 366 339 230 191 58 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 1873 2103
Size: 1873 230
BbvCI CC'TCA_GC
Cuts at: 0 1217 1240 1903 2103
Size: 1217 23 663 200
Fragments arranged by size:
1217 663 200 23
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 353 387 1168 1202 2103
Size: 353 34 781 34 901
Fragments arranged by size:
901 781 353 34 34
BglII A'GATC_T
Cuts at: 0 263 2012 2103
Size: 263 1749 91
Fragments arranged by size:
1749 263 91
BlpI GC'TnA_GC
Cuts at: 0 985 1072 2103
Size: 985 87 1031
Fragments arranged by size:
1031 985 87
Bme1580I G_kGCm'C
Cuts at: 0 172 273 473 647 2103
Size: 172 101 200 174 1456
Fragments arranged by size:
1456 200 174 172 101
BmgBI CAC'GTC
Cuts at: 0 1148 2103
Size: 1148 955
BmrI ACTGGGnnnn_n'
Cuts at: 0 149 473 780 1106 2103
Size: 149 324 307 326 997
Fragments arranged by size:
997 326 324 307 149
BmtI G_CTAG'C
Cuts at: 0 1141 2103
Size: 1141 962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 943 1219 1546 1855 2103
Size: 409 534 276 327 309 248
Fragments arranged by size:
534 409 327 309 276 248
Bpu10I CC'TnA_GC
Cuts at: 0 1217 1240 1650 1682 1903 2103
Size: 1217 23 410 32 221 200
Fragments arranged by size:
1217 410 221 200 32 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 2103
Size: 598 1505
BsaI GGTCTCn'nnnn_
Cuts at: 0 319 1831 2103
Size: 319 1512 272
Fragments arranged by size:
1512 319 272
BsaAI yAC'GTr
Cuts at: 0 759 1874 2103
Size: 759 1115 229
Fragments arranged by size:
1115 759 229
BsaBI GATnn'nnATC
Cuts at: 0 1057 2103
Size: 1057 1046
BsaHI Gr'CG_yC
Cuts at: 0 812 2103
Size: 812 1291
BsaWI w'CCGG_w
Cuts at: 0 492 1590 2103
Size: 492 1098 513
Fragments arranged by size:
1098 513 492
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 418 1267 1324 1978 1992 2103
Size: 418 849 57 654 14 111
Fragments arranged by size:
849 654 418 111 57 14
BseYI C'CCAG_C
Cuts at: 0 460 1390 1785 2103
Size: 460 930 395 318
Fragments arranged by size:
930 460 395 318
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 190 280 822 1012 1630 2103
Size: 184 6 90 542 190 618 473
Fragments arranged by size:
618 542 473 190 184 90 6
BsiEI CG_ry'CG
Cuts at: 0 282 993 2103
Size: 282 711 1110
Fragments arranged by size:
1110 711 282
BsiHKAI G_wGCw'C
Cuts at: 0 191 395 473 770 986 1421 1651 1895
Size: 191 204 78 297 216 435 230 244
Cuts at: 1895 2103
Size: 208
Fragments arranged by size:
435 297 244 230 216 208 204 191
78
BsmI GAATG_Cn'
Cuts at: 0 1524 2103
Size: 1524 579
BsmBI CGTCTCn'nnnn_
Cuts at: 0 753 2103
Size: 753 1350
Bsp1286I G_dGCh'C
Cuts at: 0 172 191 273 395 473 647 770 986
Size: 172 19 82 122 78 174 123 216
Cuts at: 986 1352 1410 1421 1651 1895 2103
Size: 366 58 11 230 244 208
Fragments arranged by size:
366 244 230 216 208 174 172 123
122 82 78 58 19 11
BspEI T'CCGG_A
Cuts at: 0 1590 2103
Size: 1590 513
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1202 1256 1484 1726 2103
Size: 1202 54 228 242 377
Fragments arranged by size:
1202 377 242 228 54
BsrBI CCG'CTC
Cuts at: 0 216 2103
Size: 216 1887
BsrDI GCAATG_nn'
Cuts at: 0 1184 1211 2103
Size: 1184 27 892
Fragments arranged by size:
1184 892 27
BsrFI r'CCGG_y
Cuts at: 0 518 2103
Size: 518 1585
BssSI C'ACGA_G
Cuts at: 0 1177 2103
Size: 1177 926
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2042 2103
Size: 2042 61
BstXI CCAn_nnnn'nTGG
Cuts at: 0 654 1084 2103
Size: 654 430 1019
Fragments arranged by size:
1019 654 430
BstYI r'GATC_y
Cuts at: 0 263 845 2012 2103
Size: 263 582 1167 91
Fragments arranged by size:
1167 582 263 91
Bsu36I CC'TnA_GG
Cuts at: 0 173 2103
Size: 173 1930
BtgI C'CryG_G
Cuts at: 0 647 669 977 1577 1972 2103
Size: 647 22 308 600 395 131
Fragments arranged by size:
647 600 395 308 131 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 490 1044 1511 2103
Size: 490 554 467 592
Fragments arranged by size:
592 554 490 467
BtsI GCAGTG_nn'
Cuts at: 0 172 1005 2103
Size: 172 833 1098
Fragments arranged by size:
1098 833 172
DraI TTT'AAA
Cuts at: 0 1623 2103
Size: 1623 480
DraIII CAC_nnn'GTG
Cuts at: 0 1959 2103
Size: 1959 144
EaeI y'GGCC_r
Cuts at: 0 2 23 279 761 944 990 1320 2103
Size: 2 21 256 482 183 46 330 783
Fragments arranged by size:
783 482 330 256 183 46 21 2
EagI C'GGCC_G
Cuts at: 0 279 990 2103
Size: 279 711 1113
Fragments arranged by size:
1113 711 279
EarI CTCTTCn'nnn_
Cuts at: 0 694 2103
Size: 694 1409
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 190 625 820 1984 2017 2103
Size: 190 435 195 1164 33 86
Fragments arranged by size:
1164 435 195 190 86 33
EcoICRI GAG'CTC
Cuts at: 0 189 984 1419 1649 2103
Size: 189 795 435 230 454
Fragments arranged by size:
795 454 435 230 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 727 943 1219 1546 1855 2103
Size: 409 318 216 276 327 309 248
Fragments arranged by size:
409 327 318 309 276 248 216
EcoNI CCTnn'n_nnAGG
Cuts at: 0 895 1239 1339 2103
Size: 895 344 100 764
Fragments arranged by size:
895 764 344 100
EcoO109I rG'GnC_Cy
Cuts at: 0 177 237 643 1473 1968 2103
Size: 177 60 406 830 495 135
Fragments arranged by size:
830 495 406 177 135 60
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 441 2103
Size: 441 1662
HaeII r_GCGC'y
Cuts at: 0 2004 2103
Size: 2004 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 49 60 81 1286 1318 2103
Size: 28 21 11 21 1205 32 785
Fragments arranged by size:
1205 785 32 28 21 21 11
HincII GTy'rAC
Cuts at: 0 816 1743 2103
Size: 816 927 360
Fragments arranged by size:
927 816 360
KpnI G_GTAC'C
Cuts at: 0 798 2103
Size: 798 1305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 1394 2103
Size: 659 735 709
Fragments arranged by size:
735 709 659
MscI TGG'CCA
Cuts at: 0 4 25 2103
Size: 4 21 2078
Fragments arranged by size:
2078 21 4
MspA1I CmG'CkG
Cuts at: 0 108 671 836 989 1974 2103
Size: 108 563 165 153 985 129
Fragments arranged by size:
985 563 165 153 129 108
NcoI C'CATG_G
Cuts at: 0 647 2103
Size: 647 1456
NheI G'CTAG_C
Cuts at: 0 1137 2103
Size: 1137 966
NspI r_CATG'y
Cuts at: 0 1368 2103
Size: 1368 735
PasI CC'CwG_GG
Cuts at: 0 1084 1371 1932 2103
Size: 1084 287 561 171
Fragments arranged by size:
1084 561 287 171
PflMI CCAn_nnn'nTGG
Cuts at: 0 312 1148 1197 2103
Size: 312 836 49 906
Fragments arranged by size:
906 836 312 49
PfoI T'CCnGG_A
Cuts at: 0 338 386 543 1469 1943 2103
Size: 338 48 157 926 474 160
Fragments arranged by size:
926 474 338 160 157 48
PmlI CAC'GTG
Cuts at: 0 1874 2103
Size: 1874 229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1999 2031 2103
Size: 1999 32 72
Fragments arranged by size:
1999 72 32
PpuMI rG'GwC_Cy
Cuts at: 0 177 237 1473 2103
Size: 177 60 1236 630
Fragments arranged by size:
1236 630 177 60
PspOMI G'GGCC_C
Cuts at: 0 643 2103
Size: 643 1460
PvuII CAG'CTG
Cuts at: 0 108 2103
Size: 108 1995
SacI G_AGCT'C
Cuts at: 0 191 986 1421 1651 2103
Size: 191 795 435 230 452
Fragments arranged by size:
795 452 435 230 191
SacII CC_GC'GG
Cuts at: 0 672 1975 2103
Size: 672 1303 128
Fragments arranged by size:
1303 672 128
SfcI C'TryA_G
Cuts at: 0 1170 1703 2103
Size: 1170 533 400
Fragments arranged by size:
1170 533 400
SmlI C'TyrA_G
Cuts at: 0 613 2103
Size: 613 1490
SspI AAT'ATT
Cuts at: 0 1619 2103
Size: 1619 484
StuI AGG'CCT
Cuts at: 0 1222 2103
Size: 1222 881
StyI C'CwwG_G
Cuts at: 0 233 241 647 1358 1660 2103
Size: 233 8 406 711 302 443
Fragments arranged by size:
711 443 406 302 233 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 172 1596 2103
Size: 172 1424 507
Fragments arranged by size:
1424 507 172
TatI w'GTAC_w
Cuts at: 0 155 2103
Size: 155 1948
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2009 2041 2103
Size: 2009 32 62
Fragments arranged by size:
2009 62 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 313 1452 1477 2103
Size: 313 1139 25 626
Fragments arranged by size:
1139 626 313 25
XmnI GAAnn'nnTTC
Cuts at: 0 724 2103
Size: 724 1379
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AlfI AlwNI ApaI
ApaLI ApoI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KpnI MmeI MscI MspA1I
NcoI NheI NspI PasI PflMI PfoI PmlI PpiI
PpuMI PspOMI PvuII SacI SacII SfcI SmlI SspI
StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AleI AloI
AscI AseI AsiSI BbeI BciVI BclI BfrBI BglI
BplI BsaXI BsiWI BspHI BsrGI BssHII BstBI BstEII
BstZ17I ClaI CspCI DrdI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KasI MfeI MluI MslI NaeI
NarI NdeI NgoMIV NotI NruI NsiI PacI PciI
PmeI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XhoI XmaI ZraI