Restriction map for
pFN21AE2054
(Linear) (Six-base) MAPSORT of: KIEE2054.seq Check: 3234 from: 1 to: 1875
KIEE2054.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AleI CACnn'nnGTG
Cuts at: 0 121 1875
Size: 121 1754
AlwNI CAG_nnn'CTG
Cuts at: 0 988 1578 1875
Size: 988 590 297
Fragments arranged by size:
988 590 297
ApoI r'AATT_y
Cuts at: 0 1050 1139 1172 1430 1875
Size: 1050 89 33 258 445
Fragments arranged by size:
1050 445 258 89 33
AvaI C'yCGr_G
Cuts at: 0 665 1208 1875
Size: 665 543 667
Fragments arranged by size:
667 665 543
BanI G'GyrC_C
Cuts at: 0 1122 1875
Size: 1122 753
BanII G_rGCy'C
Cuts at: 0 1736 1751 1875
Size: 1736 15 124
Fragments arranged by size:
1736 124 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 575 1634 1875
Size: 575 1059 241
Fragments arranged by size:
1059 575 241
BbvCI CC'TCA_GC
Cuts at: 0 1346 1875
Size: 1346 529
BclI T'GATC_A
Cuts at: 0 1320 1769 1875
Size: 1320 449 106
Fragments arranged by size:
1320 449 106
BglII A'GATC_T
Cuts at: 0 433 1790 1875
Size: 433 1357 85
Fragments arranged by size:
1357 433 85
BlpI GC'TnA_GC
Cuts at: 0 79 1745 1875
Size: 79 1666 130
Fragments arranged by size:
1666 130 79
BmgBI CAC'GTC
Cuts at: 0 234 1565 1632 1875
Size: 234 1331 67 243
Fragments arranged by size:
1331 243 234 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 410 1118 1780 1875
Size: 307 103 708 662 95
Fragments arranged by size:
708 662 307 103 95
Bpu10I CC'TnA_GC
Cuts at: 0 924 1346 1875
Size: 924 422 529
Fragments arranged by size:
924 529 422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 1801 1875
Size: 414 1387 74
Fragments arranged by size:
1387 414 74
BsaHI Gr'CG_yC
Cuts at: 0 164 1875
Size: 164 1711
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 709 739 1875
Size: 709 30 1136
Fragments arranged by size:
1136 709 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 518 1417 1875
Size: 518 899 458
Fragments arranged by size:
899 518 458
BseYI C'CCAG_C
Cuts at: 0 36 1875
Size: 36 1839
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1875
Size: 88 1787
BsiEI CG_ry'CG
Cuts at: 0 810 1875
Size: 810 1065
BsiHKAI G_wGCw'C
Cuts at: 0 860 1751 1875
Size: 860 891 124
Fragments arranged by size:
891 860 124
BsmBI CGTCTCn'nnnn_
Cuts at: 0 500 1432 1875
Size: 500 932 443
Fragments arranged by size:
932 500 443
Bsp1286I G_dGCh'C
Cuts at: 0 860 1736 1751 1875
Size: 860 876 15 124
Fragments arranged by size:
876 860 124 15
BspHI T'CATG_A
Cuts at: 0 143 1875
Size: 143 1732
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 110 1107 1875
Size: 110 997 768
Fragments arranged by size:
997 768 110
BsrBI CCG'CTC
Cuts at: 0 714 1875
Size: 714 1161
BstXI CCAn_nnnn'nTGG
Cuts at: 0 234 331 1875
Size: 234 97 1544
Fragments arranged by size:
1544 234 97
BstYI r'GATC_y
Cuts at: 0 433 1790 1875
Size: 433 1357 85
Fragments arranged by size:
1357 433 85
BtgI C'CryG_G
Cuts at: 0 1727 1875
Size: 1727 148
BtsI GCAGTG_nn'
Cuts at: 0 1391 1875
Size: 1391 484
DraI TTT'AAA
Cuts at: 0 963 1875
Size: 963 912
EaeI y'GGCC_r
Cuts at: 0 266 1104 1875
Size: 266 838 771
Fragments arranged by size:
838 771 266
EarI CTCTTCn'nnn_
Cuts at: 0 464 1307 1394 1875
Size: 464 843 87 481
Fragments arranged by size:
843 481 464 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 36 638 1875
Size: 36 602 1237
Fragments arranged by size:
1237 602 36
EcoICRI GAG'CTC
Cuts at: 0 1749 1875
Size: 1749 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 410 1118 1780 1875
Size: 307 103 708 662 95
Fragments arranged by size:
708 662 307 103 95
EcoNI CCTnn'n_nnAGG
Cuts at: 0 220 1699 1875
Size: 220 1479 176
Fragments arranged by size:
1479 220 176
EcoO109I rG'GnC_Cy
Cuts at: 0 150 867 1692 1875
Size: 150 717 825 183
Fragments arranged by size:
825 717 183 150
EcoRI G'AATT_C
Cuts at: 0 1172 1875
Size: 1172 703
HaeII r_GCGC'y
Cuts at: 0 37 1240 1875
Size: 37 1203 635
Fragments arranged by size:
1203 635 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 709 741 898 930 1141 1173
Size: 160 32 517 32 157 32 211 32
Cuts at: 1173 1875
Size: 702
Fragments arranged by size:
702 517 211 160 157 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1184 1875
Size: 1184 691
MscI TGG'CCA
Cuts at: 0 268 1106 1875
Size: 268 838 769
Fragments arranged by size:
838 769 268
MslI CAynn'nnrTG
Cuts at: 0 121 232 1875
Size: 121 111 1643
Fragments arranged by size:
1643 121 111
MspA1I CmG'CkG
Cuts at: 0 63 1767 1875
Size: 63 1704 108
Fragments arranged by size:
1704 108 63
PasI CC'CwG_GG
Cuts at: 0 1599 1875
Size: 1599 276
PflMI CCAn_nnn'nTGG
Cuts at: 0 1842 1875
Size: 1842 33
PfoI T'CCnGG_A
Cuts at: 0 1622 1875
Size: 1622 253
PpuMI rG'GwC_Cy
Cuts at: 0 867 1875
Size: 867 1008
PstI C_TGCA'G
Cuts at: 0 1658 1700 1875
Size: 1658 42 175
Fragments arranged by size:
1658 175 42
PvuII CAG'CTG
Cuts at: 0 1767 1875
Size: 1767 108
SacI G_AGCT'C
Cuts at: 0 1751 1875
Size: 1751 124
SfcI C'TryA_G
Cuts at: 0 1654 1696 1875
Size: 1654 42 179
Fragments arranged by size:
1654 179 42
SmaI CCC'GGG
Cuts at: 0 667 1210 1875
Size: 667 543 665
Fragments arranged by size:
667 665 543
SmlI C'TyrA_G
Cuts at: 0 429 1816 1875
Size: 429 1387 59
Fragments arranged by size:
1387 429 59
SrfI GCCC'GGGC
Cuts at: 0 667 1875
Size: 667 1208
StyI C'CwwG_G
Cuts at: 0 611 971 1541 1875
Size: 611 360 570 334
Fragments arranged by size:
611 570 360 334
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 812 1488 1875
Size: 812 676 387
Fragments arranged by size:
812 676 387
TatI w'GTAC_w
Cuts at: 0 529 1875
Size: 529 1346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1053 1875
Size: 1053 822
XmaI C'CCGG_G
Cuts at: 0 665 1208 1875
Size: 665 543 667
Fragments arranged by size:
667 665 543
Enzymes that do cut:
AleI AlwNI ApoI AvaI BanI BanII BbsI BbvCI
BclI BglII BlpI BmgBI BpmI Bpu10I BpuEI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BstXI BstYI BtgI BtsI DraI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I MmeI MscI MslI MspA1I PasI PflMI
PfoI PpuMI PstI PvuII SacI SfcI SmaI SmlI
SrfI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI
BfrBI BglI Bme1580I BmrI BmtI BplI BsaI BsaAI
BsaBI BsaWI BsiWI BsmI BspEI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII DrdI EagI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmnI ZraI