Restriction map for
pFN21AE2051
(Linear) (Six-base) MAPSORT of: KIEE2051.seq Check: 7766 from: 1 to: 1851
KIEE2051.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 116 1851
Size: 116 1735
AccI GT'mk_AC
Cuts at: 0 1469 1538 1851
Size: 1469 69 313
Fragments arranged by size:
1469 313 69
Acc65I G'GTAC_C
Cuts at: 0 242 1851
Size: 242 1609
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 283 1851
Size: 268 15 1568
Fragments arranged by size:
1568 268 15
AflIII A'CryG_T
Cuts at: 0 587 1197 1541 1851
Size: 587 610 344 310
Fragments arranged by size:
610 587 344 310
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 769 1851
Size: 769 1082
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 550 582 1851
Size: 550 32 1269
Fragments arranged by size:
1269 550 32
AlwNI CAG_nnn'CTG
Cuts at: 0 711 915 1851
Size: 711 204 936
Fragments arranged by size:
936 711 204
ApaLI G'TGCA_C
Cuts at: 0 1168 1851
Size: 1168 683
ApoI r'AATT_y
Cuts at: 0 371 1851
Size: 371 1480
AvaI C'yCGr_G
Cuts at: 0 359 757 993 1354 1370 1753 1851
Size: 359 398 236 361 16 383 98
Fragments arranged by size:
398 383 361 359 236 98 16
BanI G'GyrC_C
Cuts at: 0 242 1851
Size: 242 1609
BanII G_rGCy'C
Cuts at: 0 85 1427 1851
Size: 85 1342 424
Fragments arranged by size:
1342 424 85
BbsI GAAGACnn'nnnn_
Cuts at: 0 271 590 692 1397 1851
Size: 271 319 102 705 454
Fragments arranged by size:
705 454 319 271 102
BbvCI CC'TCA_GC
Cuts at: 0 70 1851
Size: 70 1781
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1734 1768 1851
Size: 1734 34 83
Fragments arranged by size:
1734 83 34
BglI GCCn_nnn'nGGC
Cuts at: 0 80 1366 1851
Size: 80 1286 485
Fragments arranged by size:
1286 485 80
BlpI GC'TnA_GC
Cuts at: 0 811 1778 1851
Size: 811 967 73
Fragments arranged by size:
967 811 73
Bme1580I G_kGCm'C
Cuts at: 0 1172 1298 1851
Size: 1172 126 553
Fragments arranged by size:
1172 553 126
BmgBI CAC'GTC
Cuts at: 0 165 1083 1851
Size: 165 918 768
Fragments arranged by size:
918 768 165
BmrI ACTGGGnnnn_n'
Cuts at: 0 193 1851
Size: 193 1658
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 892 1851
Size: 820 72 959
Fragments arranged by size:
959 820 72
Bpu10I CC'TnA_GC
Cuts at: 0 70 1851
Size: 70 1781
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 480 1851
Size: 367 113 1371
Fragments arranged by size:
1371 367 113
BsaAI yAC'GTr
Cuts at: 0 1200 1299 1542 1851
Size: 1200 99 243 309
Fragments arranged by size:
1200 309 243 99
BsaHI Gr'CG_yC
Cuts at: 0 268 1851
Size: 268 1583
BsaWI w'CCGG_w
Cuts at: 0 1826 1851
Size: 1826 25
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 550 580 1591 1621 1851
Size: 550 30 1011 30 230
Fragments arranged by size:
1011 550 230 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1516 1851
Size: 1516 335
BseYI C'CCAG_C
Cuts at: 0 1646 1851
Size: 1646 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 729 813 885 1321 1330 1851
Size: 729 84 72 436 9 521
Fragments arranged by size:
729 521 436 84 72 9
BsiEI CG_ry'CG
Cuts at: 0 1460 1851
Size: 1460 391
BsiHKAI G_wGCw'C
Cuts at: 0 1172 1851
Size: 1172 679
Bsp1286I G_dGCh'C
Cuts at: 0 85 1172 1298 1427 1851
Size: 85 1087 126 129 424
Fragments arranged by size:
1087 424 129 126 85
BspHI T'CATG_A
Cuts at: 0 668 1851
Size: 668 1183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 116 1851
Size: 116 1735
BsrBI CCG'CTC
Cuts at: 0 663 804 922 1851
Size: 663 141 118 929
Fragments arranged by size:
929 663 141 118
BsrDI GCAATG_nn'
Cuts at: 0 968 1139 1151 1211 1851
Size: 968 171 12 60 640
Fragments arranged by size:
968 640 171 60 12
BsrFI r'CCGG_y
Cuts at: 0 46 313 1851
Size: 46 267 1538
Fragments arranged by size:
1538 267 46
BssSI C'ACGA_G
Cuts at: 0 76 1851
Size: 76 1775
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1308 1851
Size: 1308 543
BstEII G'GTnAC_C
Cuts at: 0 15 771 1851
Size: 15 756 1080
Fragments arranged by size:
1080 756 15
BstXI CCAn_nnnn'nTGG
Cuts at: 0 555 783 1851
Size: 555 228 1068
Fragments arranged by size:
1068 555 228
BstYI r'GATC_y
Cuts at: 0 191 1851
Size: 191 1660
Bsu36I CC'TnA_GG
Cuts at: 0 334 1569 1851
Size: 334 1235 282
Fragments arranged by size:
1235 334 282
BtgI C'CryG_G
Cuts at: 0 20 273 776 1622 1655 1730 1851
Size: 20 253 503 846 33 75 121
Fragments arranged by size:
846 503 253 121 75 33 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 332 568 1851
Size: 332 236 1283
Fragments arranged by size:
1283 332 236
ClaI AT'CG_AT
Cuts at: 0 429 467 878 1851
Size: 429 38 411 973
Fragments arranged by size:
973 429 411 38
DrdI GACnn_nn'nnGTC
Cuts at: 0 1081 1851
Size: 1081 770
EaeI y'GGCC_r
Cuts at: 0 23 917 950 1049 1457 1668 1844 1851
Size: 23 894 33 99 408 211 176 7
Fragments arranged by size:
894 408 211 176 99 33 23 7
EagI C'GGCC_G
Cuts at: 0 1457 1851
Size: 1457 394
EarI CTCTTCn'nnn_
Cuts at: 0 1691 1851
Size: 1691 160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1632 1851
Size: 1632 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 283 820 892 1851
Size: 268 15 537 72 959
Fragments arranged by size:
959 537 268 72 15
EcoO109I rG'GnC_Cy
Cuts at: 0 1326 1506 1851
Size: 1326 180 345
Fragments arranged by size:
1326 345 180
EcoRV GAT'ATC
Cuts at: 0 501 1851
Size: 501 1350
HaeII r_GCGC'y
Cuts at: 0 744 1851
Size: 744 1107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1591 1623 1851
Size: 1591 32 228
Fragments arranged by size:
1591 228 32
HincII GTy'rAC
Cuts at: 0 1662 1851
Size: 1662 189
KpnI G_GTAC'C
Cuts at: 0 246 1851
Size: 246 1605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1589 1851
Size: 1589 262
MscI TGG'CCA
Cuts at: 0 25 952 1051 1846 1851
Size: 25 927 99 795 5
Fragments arranged by size:
927 795 99 25 5
MslI CAynn'nnrTG
Cuts at: 0 586 781 1144 1851
Size: 586 195 363 707
Fragments arranged by size:
707 586 363 195
MspA1I CmG'CkG
Cuts at: 0 198 305 1158 1605 1657 1674 1800 1851
Size: 198 107 853 447 52 17 126 51
Fragments arranged by size:
853 447 198 126 107 52 51 17
NaeI GCC'GGC
Cuts at: 0 48 1851
Size: 48 1803
NcoI C'CATG_G
Cuts at: 0 1730 1851
Size: 1730 121
NgoMIV G'CCGG_C
Cuts at: 0 46 1851
Size: 46 1805
NspI r_CATG'y
Cuts at: 0 591 1554 1689 1851
Size: 591 963 135 162
Fragments arranged by size:
963 591 162 135
PciI A'CATG_T
Cuts at: 0 587 1851
Size: 587 1264
PflMI CCAn_nnn'nTGG
Cuts at: 0 915 1058 1497 1851
Size: 915 143 439 354
Fragments arranged by size:
915 439 354 143
PmlI CAC'GTG
Cuts at: 0 1200 1299 1851
Size: 1200 99 552
Fragments arranged by size:
1200 552 99
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 550 582 1851
Size: 550 32 1269
Fragments arranged by size:
1269 550 32
PpuMI rG'GwC_Cy
Cuts at: 0 1326 1506 1851
Size: 1326 180 345
Fragments arranged by size:
1326 345 180
PspXI vC'TCGA_Gb
Cuts at: 0 1753 1851
Size: 1753 98
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1522 1554 1851
Size: 1522 32 297
Fragments arranged by size:
1522 297 32
PstI C_TGCA'G
Cuts at: 0 97 1520 1851
Size: 97 1423 331
Fragments arranged by size:
1423 331 97
PvuII CAG'CTG
Cuts at: 0 198 1158 1605 1674 1800 1851
Size: 198 960 447 69 126 51
Fragments arranged by size:
960 447 198 126 69 51
SacII CC_GC'GG
Cuts at: 0 1658 1851
Size: 1658 193
SapI GCTCTTCn'nnn_
Cuts at: 0 1691 1851
Size: 1691 160
ScaI AGT'ACT
Cuts at: 0 223 1851
Size: 223 1628
SexAI A'CCwGG_T
Cuts at: 0 1490 1851
Size: 1490 361
SfcI C'TryA_G
Cuts at: 0 93 1516 1851
Size: 93 1423 335
Fragments arranged by size:
1423 335 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1366 1851
Size: 1366 485
SgrAI Cr'CCGG_yG
Cuts at: 0 313 1851
Size: 313 1538
SmaI CCC'GGG
Cuts at: 0 995 1372 1851
Size: 995 377 479
Fragments arranged by size:
995 479 377
SmlI C'TyrA_G
Cuts at: 0 382 459 1753 1851
Size: 382 77 1294 98
Fragments arranged by size:
1294 382 98 77
SphI G_CATG'C
Cuts at: 0 1554 1851
Size: 1554 297
StyI C'CwwG_G
Cuts at: 0 1226 1730 1851
Size: 1226 504 121
Fragments arranged by size:
1226 504 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1050 1114 1575 1851
Size: 1050 64 461 276
Fragments arranged by size:
1050 461 276 64
TatI w'GTAC_w
Cuts at: 0 221 251 1851
Size: 221 30 1600
Fragments arranged by size:
1600 221 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 896 928 1851
Size: 896 32 923
Fragments arranged by size:
923 896 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 712 1851
Size: 712 1139
XhoI C'TCGA_G
Cuts at: 0 1753 1851
Size: 1753 98
XmaI C'CCGG_G
Cuts at: 0 993 1370 1851
Size: 993 377 481
Fragments arranged by size:
993 481 377
XmnI GAAnn'nnTTC
Cuts at: 0 565 610 844 1851
Size: 565 45 234 1007
Fragments arranged by size:
1007 565 234 45
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlfI AloI AlwNI
ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI ClaI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRV
HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PciI PflMI PmlI PpiI
PpuMI PspXI PsrI PstI PvuII SacII SapI ScaI
SexAI SfcI SfiI SgrAI SmaI SmlI SphI StyI
TaqII TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI
BclI BfrBI BglII BmtI BplI BsaI BsaBI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstBI BstZ17I BtsI
CspCI DraI DraIII EcoICRI EcoNI EcoRI FalI FseI
FspI FspAI HindIII HpaI KasI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmeI PshAI PsiI PspOMI PvuI RsrII SacI SalI
SanDI SbfI SfoI SgfI SnaBI SpeI SrfI SspI
StuI SwaI Tth111I XbaI ZraI