Restriction map for
pFN21AE2050
(Linear) (Six-base) MAPSORT of: KIEE2050.seq Check: 4699 from: 1 to: 2013
KIEE2050.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AclI AA'CG_TT
Cuts at: 0 1746 2013
Size: 1746 267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 626 697 1066 2013
Size: 352 274 71 369 947
Fragments arranged by size:
947 369 352 274 71
AgeI A'CCGG_T
Cuts at: 0 56 2013
Size: 56 1957
AleI CACnn'nnGTG
Cuts at: 0 217 2013
Size: 217 1796
AlwNI CAG_nnn'CTG
Cuts at: 0 34 1903 2013
Size: 34 1869 110
Fragments arranged by size:
1869 110 34
ApaLI G'TGCA_C
Cuts at: 0 551 2013
Size: 551 1462
ApoI r'AATT_y
Cuts at: 0 919 1087 1148 1566 2013
Size: 919 168 61 418 447
Fragments arranged by size:
919 447 418 168 61
AvaI C'yCGr_G
Cuts at: 0 1003 2013
Size: 1003 1010
BanII G_rGCy'C
Cuts at: 0 1956 2013
Size: 1956 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 301 641 662 1275 2013
Size: 301 340 21 613 738
Fragments arranged by size:
738 613 340 301 21
BglI GCCn_nnn'nGGC
Cuts at: 0 71 2013
Size: 71 1942
BglII A'GATC_T
Cuts at: 0 399 609 2013
Size: 399 210 1404
Fragments arranged by size:
1404 399 210
BlpI GC'TnA_GC
Cuts at: 0 384 2013
Size: 384 1629
Bme1580I G_kGCm'C
Cuts at: 0 555 2013
Size: 555 1458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1634 1940 2013
Size: 1634 306 73
Fragments arranged by size:
1634 306 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1139 2013
Size: 76 1063 874
Fragments arranged by size:
1063 874 76
BsaBI GATnn'nnATC
Cuts at: 0 1100 2013
Size: 1100 913
BsaWI w'CCGG_w
Cuts at: 0 56 2013
Size: 56 1957
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 223 253 2013
Size: 223 30 1760
Fragments arranged by size:
1760 223 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 448 742 1213 2013
Size: 448 294 471 800
Fragments arranged by size:
800 471 448 294
BseYI C'CCAG_C
Cuts at: 0 1037 2013
Size: 1037 976
BsiHKAI G_wGCw'C
Cuts at: 0 555 2013
Size: 555 1458
BsmI GAATG_Cn'
Cuts at: 0 291 385 2013
Size: 291 94 1628
Fragments arranged by size:
1628 291 94
Bsp1286I G_dGCh'C
Cuts at: 0 555 1956 2013
Size: 555 1401 57
Fragments arranged by size:
1401 555 57
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 42 1953 2013
Size: 42 1911 60
Fragments arranged by size:
1911 60 42
BsrBI CCG'CTC
Cuts at: 0 8 2013
Size: 8 2005
BsrFI r'CCGG_y
Cuts at: 0 56 2013
Size: 56 1957
BsrGI T'GTAC_A
Cuts at: 0 944 2013
Size: 944 1069
BssHII G'CGCG_C
Cuts at: 0 84 2013
Size: 84 1929
BstAPI GCAn_nnn'nTGC
Cuts at: 0 463 2013
Size: 463 1550
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1727 2013
Size: 1727 286
BstYI r'GATC_y
Cuts at: 0 399 609 1482 1716 2013
Size: 399 210 873 234 297
Fragments arranged by size:
873 399 297 234 210
BtgI C'CryG_G
Cuts at: 0 1133 2013
Size: 1133 880
BtsI GCAGTG_nn'
Cuts at: 0 395 542 1893 2013
Size: 395 147 1351 120
Fragments arranged by size:
1351 395 147 120
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 758 793 1399 1434 2013
Size: 758 35 606 35 579
Fragments arranged by size:
758 606 579 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 832 2013
Size: 832 1181
EaeI y'GGCC_r
Cuts at: 0 63 674 2013
Size: 63 611 1339
Fragments arranged by size:
1339 611 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1398 2013
Size: 1398 615
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 626 697 1066 1634 1940 2013
Size: 352 274 71 369 568 306 73
Fragments arranged by size:
568 369 352 306 274 73 71
EcoO109I rG'GnC_Cy
Cuts at: 0 1802 2013
Size: 1802 211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 952 2013
Size: 952 1061
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 389 421 1519 1551 2013
Size: 389 32 1098 32 462
Fragments arranged by size:
1098 462 389 32 32
HincII GTy'rAC
Cuts at: 0 1624 2013
Size: 1624 389
HindIII A'AGCT_T
Cuts at: 0 188 889 929 2013
Size: 188 701 40 1084
Fragments arranged by size:
1084 701 188 40
MfeI C'AATT_G
Cuts at: 0 477 2013
Size: 477 1536
MscI TGG'CCA
Cuts at: 0 65 676 2013
Size: 65 611 1337
Fragments arranged by size:
1337 611 65
MslI CAynn'nnrTG
Cuts at: 0 217 2013
Size: 217 1796
MspA1I CmG'CkG
Cuts at: 0 17 573 1117 1416 2013
Size: 17 556 544 299 597
Fragments arranged by size:
597 556 544 299 17
NcoI C'CATG_G
Cuts at: 0 1133 2013
Size: 1133 880
NspI r_CATG'y
Cuts at: 0 551 1178 2013
Size: 551 627 835
Fragments arranged by size:
835 627 551
PasI CC'CwG_GG
Cuts at: 0 1806 2013
Size: 1806 207
PflMI CCAn_nnn'nTGG
Cuts at: 0 1882 2013
Size: 1882 131
PpuMI rG'GwC_Cy
Cuts at: 0 1802 2013
Size: 1802 211
PstI C_TGCA'G
Cuts at: 0 710 1821 1950 2013
Size: 710 1111 129 63
Fragments arranged by size:
1111 710 129 63
PvuII CAG'CTG
Cuts at: 0 573 1117 1416 2013
Size: 573 544 299 597
Fragments arranged by size:
597 573 544 299
SanDI GG'GwC_CC
Cuts at: 0 1802 2013
Size: 1802 211
SbfI CC_TGCA'GG
Cuts at: 0 1950 2013
Size: 1950 63
SfcI C'TryA_G
Cuts at: 0 112 706 914 1817 1946 2013
Size: 112 594 208 903 129 67
Fragments arranged by size:
903 594 208 129 112 67
SmlI C'TyrA_G
Cuts at: 0 91 1154 2013
Size: 91 1063 859
Fragments arranged by size:
1063 859 91
SspI AAT'ATT
Cuts at: 0 1164 1184 2013
Size: 1164 20 829
Fragments arranged by size:
1164 829 20
StyI C'CwwG_G
Cuts at: 0 564 1133 2013
Size: 564 569 880
Fragments arranged by size:
880 569 564
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1881 2013
Size: 1881 132
TatI w'GTAC_w
Cuts at: 0 263 944 2013
Size: 263 681 1069
Fragments arranged by size:
1069 681 263
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1280 1312 1584 1616 2013
Size: 1280 32 272 32 397
Fragments arranged by size:
1280 397 272 32 32
Enzymes that do cut:
AclI AcuI AgeI AleI AlwNI ApaLI ApoI AvaI
BanII BbsI BglI BglII BlpI Bme1580I BpmI BpuEI
BsaBI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstXI BstYI
BtgI BtsI CspCI DrdI EaeI EciI Eco57MI EcoO109I
FalI Hin4I HincII HindIII MfeI MscI MslI MspA1I
NcoI NspI PasI PflMI PpuMI PstI PvuII SanDI
SbfI SfcI SmlI SspI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AhdI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaHI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII
EagI EarI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HpaI KasI KpnI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI