Restriction map for
pFN21AE2048
(Linear) (Six-base) MAPSORT of: KIEE2048.seq Check: 195 from: 1 to: 2007
KIEE2048.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AccI GT'mk_AC
Cuts at: 0 23 2007
Size: 23 1984
AflIII A'CryG_T
Cuts at: 0 309 341 2007
Size: 309 32 1666
Fragments arranged by size:
1666 309 32
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 664 2007
Size: 664 1343
AlwNI CAG_nnn'CTG
Cuts at: 0 282 508 1318 2007
Size: 282 226 810 689
Fragments arranged by size:
810 689 282 226
ApaI G_GGCC'C
Cuts at: 0 717 753 1158 1389 1428 1518 2007
Size: 717 36 405 231 39 90 489
Fragments arranged by size:
717 489 405 231 90 39 36
ApaLI G'TGCA_C
Cuts at: 0 118 2007
Size: 118 1889
AvaI C'yCGr_G
Cuts at: 0 665 1751 2007
Size: 665 1086 256
Fragments arranged by size:
1086 665 256
BanI G'GyrC_C
Cuts at: 0 846 1045 1495 2007
Size: 846 199 450 512
Fragments arranged by size:
846 512 450 199
BanII G_rGCy'C
Cuts at: 0 717 753 924 999 1137 1158 1191 1389
Size: 717 36 171 75 138 21 33 198
Cuts at: 1389 1428 1464 1518 1832 1951 2007
Size: 39 36 54 314 119 56
Fragments arranged by size:
717 314 198 171 138 119 75 56
54 39 36 36 33 21
BbeI G_GCGC'C
Cuts at: 0 1049 2007
Size: 1049 958
BbvCI CC'TCA_GC
Cuts at: 0 930 2007
Size: 930 1077
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 276 310 959 993 1925 1959 2007
Size: 276 34 649 34 932 34 48
Fragments arranged by size:
932 649 276 48 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 27 507 2007
Size: 27 480 1500
Fragments arranged by size:
1500 480 27
BglI GCCn_nnn'nGGC
Cuts at: 0 748 1297 1448 1513 1534 1570 1631 2007
Size: 748 549 151 65 21 36 61 376
Fragments arranged by size:
748 549 376 151 65 61 36 21
BlpI GC'TnA_GC
Cuts at: 0 824 2007
Size: 824 1183
Bme1580I G_kGCm'C
Cuts at: 0 122 717 753 849 1158 1389 1428 1518
Size: 122 595 36 96 309 231 39 90
Cuts at: 1518 2007
Size: 489
Fragments arranged by size:
595 489 309 231 122 96 90 39
36
BmgBI CAC'GTC
Cuts at: 0 327 2007
Size: 327 1680
BmrI ACTGGGnnnn_n'
Cuts at: 0 742 876 1152 1230 2007
Size: 742 134 276 78 777
Fragments arranged by size:
777 742 276 134 78
Bpu10I CC'TnA_GC
Cuts at: 0 930 998 1136 2007
Size: 930 68 138 871
Fragments arranged by size:
930 871 138 68
BsaHI Gr'CG_yC
Cuts at: 0 1046 2007
Size: 1046 961
BsaWI w'CCGG_w
Cuts at: 0 42 2007
Size: 42 1965
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 674 1161 2007
Size: 61 613 487 846
Fragments arranged by size:
846 613 487 61
BseYI C'CCAG_C
Cuts at: 0 508 695 716 752 923 1211 1388 1406
Size: 508 187 21 36 171 288 177 18
Cuts at: 1406 1589 2007
Size: 183 418
Fragments arranged by size:
508 418 288 187 183 177 171 36
21 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 1097 1238 1420 2007
Size: 148 949 141 182 587
Fragments arranged by size:
949 587 182 148 141
BsiEI CG_ry'CG
Cuts at: 0 184 639 1815 2007
Size: 184 455 1176 192
Fragments arranged by size:
1176 455 192 184
BsiHKAI G_wGCw'C
Cuts at: 0 122 1832 2007
Size: 122 1710 175
Fragments arranged by size:
1710 175 122
Bsp1286I G_dGCh'C
Cuts at: 0 122 717 753 849 924 999 1137 1158
Size: 122 595 36 96 75 75 138 21
Cuts at: 1158 1191 1389 1428 1464 1518 1832 1951 2007
Size: 33 198 39 36 54 314 119 56
Fragments arranged by size:
595 314 198 138 122 119 96 75
75 56 54 39 36 36 33 21
BspEI T'CCGG_A
Cuts at: 0 42 2007
Size: 42 1965
BspHI T'CATG_A
Cuts at: 0 83 98 2007
Size: 83 15 1909
Fragments arranged by size:
1909 83 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1293 1642 1973 2007
Size: 1293 349 331 34
Fragments arranged by size:
1293 349 331 34
BsrBI CCG'CTC
Cuts at: 0 1877 2007
Size: 1877 130
BsrDI GCAATG_nn'
Cuts at: 0 258 1641 2007
Size: 258 1383 366
Fragments arranged by size:
1383 366 258
BsrFI r'CCGG_y
Cuts at: 0 1392 1814 2007
Size: 1392 422 193
Fragments arranged by size:
1392 422 193
BssHII G'CGCG_C
Cuts at: 0 140 147 189 191 2007
Size: 140 7 42 2 1816
Fragments arranged by size:
1816 140 42 7 2
BssSI C'ACGA_G
Cuts at: 0 172 196 270 2007
Size: 172 24 74 1737
Fragments arranged by size:
1737 172 74 24
BstEII G'GTnAC_C
Cuts at: 0 1024 2007
Size: 1024 983
Bsu36I CC'TnA_GG
Cuts at: 0 1119 2007
Size: 1119 888
BtgI C'CryG_G
Cuts at: 0 303 1620 2007
Size: 303 1317 387
Fragments arranged by size:
1317 387 303
EaeI y'GGCC_r
Cuts at: 0 168 181 1478 1623 2007
Size: 168 13 1297 145 384
Fragments arranged by size:
1297 384 168 145 13
EagI C'GGCC_G
Cuts at: 0 181 2007
Size: 181 1826
EarI CTCTTCn'nnn_
Cuts at: 0 298 1065 1281 2007
Size: 298 767 216 726
Fragments arranged by size:
767 726 298 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 46 1861 2007
Size: 46 1815 146
Fragments arranged by size:
1815 146 46
EcoICRI GAG'CTC
Cuts at: 0 1830 2007
Size: 1830 177
EcoO109I rG'GnC_Cy
Cuts at: 0 460 749 1154 1385 1424 1514 2007
Size: 460 289 405 231 39 90 493
Fragments arranged by size:
493 460 405 289 231 90 39
FspI TGC'GCA
Cuts at: 0 286 2007
Size: 286 1721
HaeII r_GCGC'y
Cuts at: 0 303 1049 2001 2007
Size: 303 746 952 6
Fragments arranged by size:
952 746 303 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 299 331 2007
Size: 299 32 1676
Fragments arranged by size:
1676 299 32
HincII GTy'rAC
Cuts at: 0 24 2007
Size: 24 1983
KasI G'GCGC_C
Cuts at: 0 1045 2007
Size: 1045 962
MluI A'CGCG_T
Cuts at: 0 309 341 2007
Size: 309 32 1666
Fragments arranged by size:
1666 309 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1835 2007
Size: 1835 172
MslI CAynn'nnrTG
Cuts at: 0 82 427 2007
Size: 82 345 1580
Fragments arranged by size:
1580 345 82
MspA1I CmG'CkG
Cuts at: 0 305 864 967 1081 1315 1410 1622 2007
Size: 305 559 103 114 234 95 212 385
Fragments arranged by size:
559 385 305 234 212 114 103 95
NarI GG'CG_CC
Cuts at: 0 1046 2007
Size: 1046 961
NdeI CA'TA_TG
Cuts at: 0 912 1248 2007
Size: 912 336 759
Fragments arranged by size:
912 759 336
NruI TCG'CGA
Cuts at: 0 635 2007
Size: 635 1372
NspI r_CATG'y
Cuts at: 0 126 269 2007
Size: 126 143 1738
Fragments arranged by size:
1738 143 126
PasI CC'CwG_GG
Cuts at: 0 1101 1686 2007
Size: 1101 585 321
Fragments arranged by size:
1101 585 321
PflMI CCAn_nnn'nTGG
Cuts at: 0 508 916 1372 2007
Size: 508 408 456 635
Fragments arranged by size:
635 508 456 408
PspOMI G'GGCC_C
Cuts at: 0 713 749 1154 1385 1424 1514 2007
Size: 713 36 405 231 39 90 493
Fragments arranged by size:
713 493 405 231 90 39 36
PstI C_TGCA'G
Cuts at: 0 894 972 1167 1668 2007
Size: 894 78 195 501 339
Fragments arranged by size:
894 501 339 195 78
PvuII CAG'CTG
Cuts at: 0 864 967 1081 1315 1410 2007
Size: 864 103 114 234 95 597
Fragments arranged by size:
864 597 234 114 103 95
SacI G_AGCT'C
Cuts at: 0 1832 2007
Size: 1832 175
SacII CC_GC'GG
Cuts at: 0 306 1623 2007
Size: 306 1317 384
Fragments arranged by size:
1317 384 306
SalI G'TCGA_C
Cuts at: 0 22 2007
Size: 22 1985
SfcI C'TryA_G
Cuts at: 0 744 890 968 1163 1664 2007
Size: 744 146 78 195 501 343
Fragments arranged by size:
744 501 343 195 146 78
SfoI GGC'GCC
Cuts at: 0 1047 2007
Size: 1047 960
SmaI CCC'GGG
Cuts at: 0 1753 2007
Size: 1753 254
SphI G_CATG'C
Cuts at: 0 269 2007
Size: 269 1738
StuI AGG'CCT
Cuts at: 0 222 2007
Size: 222 1785
StyI C'CwwG_G
Cuts at: 0 217 443 840 2007
Size: 217 226 397 1167
Fragments arranged by size:
1167 397 226 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 760 1114 2007
Size: 760 354 893
Fragments arranged by size:
893 760 354
Tth111I GACn'n_nGTC
Cuts at: 0 1887 2007
Size: 1887 120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 568 1365 2007
Size: 568 797 642
Fragments arranged by size:
797 642 568
XmaI C'CCGG_G
Cuts at: 0 1751 2007
Size: 1751 256
XmnI GAAnn'nnTTC
Cuts at: 0 7 106 241 2007
Size: 7 99 135 1766
Fragments arranged by size:
1766 135 99 7
Enzymes that do cut:
AccI AflIII AlfI AlwNI ApaI ApaLI AvaI BanI
BanII BbeI BbvCI BcgI BciVI BglI BlpI Bme1580I
BmgBI BmrI Bpu10I BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstEII Bsu36I BtgI EaeI EagI
EarI EciI EcoICRI EcoO109I FspI HaeII Hin4I HincII
KasI MluI MmeI MslI MspA1I NarI NdeI NruI
NspI PasI PflMI PspOMI PstI PvuII SacI SacII
SalI SfcI SfoI SmaI SphI StuI StyI TaqII
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BclI BfrBI BglII BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaXI BsiWI BsmI
BsmBI BsrGI BstAPI BstBI BstXI BstYI BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI Eco57MI EcoNI
EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI
MfeI MscI NaeI NcoI NgoMIV NheI NotI NsiI
PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI SwaI TatI TstI XbaI XhoI ZraI