Restriction map for
pFN21AE2046
(Linear) (Six-base) MAPSORT of: KIEE2046.seq Check: 9102 from: 1 to: 1992
KIEE2046.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1140 1992
Size: 1140 852
AflII C'TTAA_G
Cuts at: 0 54 1792 1992
Size: 54 1738 200
Fragments arranged by size:
1738 200 54
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 823 1992
Size: 823 1169
AlwNI CAG_nnn'CTG
Cuts at: 0 132 1992
Size: 132 1860
ApoI r'AATT_y
Cuts at: 0 351 701 892 1937 1992
Size: 351 350 191 1045 55
Fragments arranged by size:
1045 351 350 191 55
AseI AT'TA_AT
Cuts at: 0 1679 1992
Size: 1679 313
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1592 1625 1992
Size: 1592 33 367
Fragments arranged by size:
1592 367 33
BamHI G'GATC_C
Cuts at: 0 1843 1992
Size: 1843 149
BanI G'GyrC_C
Cuts at: 0 1787 1909 1992
Size: 1787 122 83
Fragments arranged by size:
1787 122 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 589 1992
Size: 589 1403
BciVI GTATCCnnnnn_n'
Cuts at: 0 123 1992
Size: 123 1869
BclI T'GATC_A
Cuts at: 0 663 1992
Size: 663 1329
BfrBI ATG'CAT
Cuts at: 0 1360 1992
Size: 1360 632
Bme1580I G_kGCm'C
Cuts at: 0 1154 1992
Size: 1154 838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1129 1992
Size: 1129 863
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 1751 1992
Size: 374 1377 241
Fragments arranged by size:
1377 374 241
BsaI GGTCTCn'nnnn_
Cuts at: 0 1473 1992
Size: 1473 519
BsaAI yAC'GTr
Cuts at: 0 884 1992
Size: 884 1108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 616 646 1102 1132 1992
Size: 616 30 456 30 860
Fragments arranged by size:
860 616 456 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 454 457 1600 1992
Size: 454 3 1143 392
Fragments arranged by size:
1143 454 392 3
BseYI C'CCAG_C
Cuts at: 0 1147 1992
Size: 1147 845
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 906 1630 1992
Size: 388 518 724 362
Fragments arranged by size:
724 518 388 362
Bsp1286I G_dGCh'C
Cuts at: 0 1154 1992
Size: 1154 838
BsrBI CCG'CTC
Cuts at: 0 709 1992
Size: 709 1283
BsrDI GCAATG_nn'
Cuts at: 0 800 1406 1520 1992
Size: 800 606 114 472
Fragments arranged by size:
800 606 472 114
BstAPI GCAn_nnn'nTGC
Cuts at: 0 84 1992
Size: 84 1908
BstYI r'GATC_y
Cuts at: 0 1843 1992
Size: 1843 149
BtgI C'CryG_G
Cuts at: 0 873 1992
Size: 873 1119
BtsI GCAGTG_nn'
Cuts at: 0 376 812 1386 1992
Size: 376 436 574 606
Fragments arranged by size:
606 574 436 376
DraIII CAC_nnn'GTG
Cuts at: 0 1191 1992
Size: 1191 801
EaeI y'GGCC_r
Cuts at: 0 1180 1992
Size: 1180 812
EarI CTCTTCn'nnn_
Cuts at: 0 516 1133 1992
Size: 516 617 859
Fragments arranged by size:
859 617 516
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1129 1140 1992
Size: 1129 11 852
Fragments arranged by size:
1129 852 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 695 1808 1992
Size: 695 1113 184
Fragments arranged by size:
1113 695 184
EcoO109I rG'GnC_Cy
Cuts at: 0 1851 1992
Size: 1851 141
EcoRI G'AATT_C
Cuts at: 0 701 1937 1992
Size: 701 1236 55
Fragments arranged by size:
1236 701 55
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 69 1992
Size: 69 1923
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 320 352 663 695 735 767
Size: 97 32 191 32 311 32 40 32
Cuts at: 767 1645 1677 1992
Size: 878 32 315
Fragments arranged by size:
878 315 311 191 97 40 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1746 1992
Size: 1746 246
HindIII A'AGCT_T
Cuts at: 0 615 1992
Size: 615 1377
HpaI GTT'AAC
Cuts at: 0 1746 1992
Size: 1746 246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 808 930 1992
Size: 151 657 122 1062
Fragments arranged by size:
1062 657 151 122
MslI CAynn'nnrTG
Cuts at: 0 794 805 1992
Size: 794 11 1187
Fragments arranged by size:
1187 794 11
MspA1I CmG'CkG
Cuts at: 0 180 1992
Size: 180 1812
NcoI C'CATG_G
Cuts at: 0 873 1992
Size: 873 1119
NsiI A_TGCA'T
Cuts at: 0 1362 1992
Size: 1362 630
PflMI CCAn_nnn'nTGG
Cuts at: 0 589 1992
Size: 589 1403
PmlI CAC'GTG
Cuts at: 0 884 1992
Size: 884 1108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 1992
Size: 34 32 1926
Fragments arranged by size:
1926 34 32
PpuMI rG'GwC_Cy
Cuts at: 0 1851 1992
Size: 1851 141
PshAI GACnn'nnGTC
Cuts at: 0 1856 1992
Size: 1856 136
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 975 1007 1992
Size: 975 32 985
Fragments arranged by size:
985 975 32
PstI C_TGCA'G
Cuts at: 0 1918 1992
Size: 1918 74
PvuII CAG'CTG
Cuts at: 0 180 1992
Size: 180 1812
SbfI CC_TGCA'GG
Cuts at: 0 1918 1992
Size: 1918 74
SexAI A'CCwGG_T
Cuts at: 0 1482 1992
Size: 1482 510
SfcI C'TryA_G
Cuts at: 0 266 1026 1914 1992
Size: 266 760 888 78
Fragments arranged by size:
888 760 266 78
SmlI C'TyrA_G
Cuts at: 0 54 389 1766 1792 1992
Size: 54 335 1377 26 200
Fragments arranged by size:
1377 335 200 54 26
StuI AGG'CCT
Cuts at: 0 387 1992
Size: 387 1605
StyI C'CwwG_G
Cuts at: 0 873 1177 1572 1992
Size: 873 304 395 420
Fragments arranged by size:
873 420 395 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 806 1992
Size: 806 1186
TatI w'GTAC_w
Cuts at: 0 1773 1992
Size: 1773 219
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1911 1943 1992
Size: 1911 32 49
Fragments arranged by size:
1911 49 32
XbaI T'CTAG_A
Cuts at: 0 856 1992
Size: 856 1136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 129 150 1992
Size: 129 21 1842
Fragments arranged by size:
1842 129 21
XmnI GAAnn'nnTTC
Cuts at: 0 49 1466 1992
Size: 49 1417 526
Fragments arranged by size:
1417 526 49
Enzymes that do cut:
AcuI AflII AlfI AlwNI ApoI AseI BaeI BamHI
BanI BbsI BciVI BclI BfrBI Bme1580I BpmI BpuEI
BsaI BsaAI BsaXI BseRI BseYI BsgI Bsp1286I BsrBI
BsrDI BstAPI BstYI BtgI BtsI DraIII EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII
HpaI MmeI MslI MspA1I NcoI NsiI PflMI PmlI
PpiI PpuMI PshAI PsrI PstI PvuII SbfI SexAI
SfcI SmlI StuI StyI TaqII TatI TstI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BanII BbeI BbvCI BcgI BglI BglII BlpI
BmgBI BmrI BmtI BplI Bpu10I BsaBI BsaHI BsaWI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoICRI EcoRV FseI FspI FspAI HaeII KasI KpnI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NspI PacI PasI PciI PfoI PmeI
PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XhoI
XmaI ZraI