Restriction map for
pFN21AE2044
(Linear) (Six-base) MAPSORT of: KIEE2044.seq Check: 7779 from: 1 to: 1986
KIEE2044.orf
With 182 enzymes: SgfI *
July 3, 2012 17:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1153 1545 1986
Size: 1153 392 441
Fragments arranged by size:
1153 441 392
AccI GT'mk_AC
Cuts at: 0 1291 1986
Size: 1291 695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1593 1986
Size: 1593 393
AfeI AGC'GCT
Cuts at: 0 1606 1986
Size: 1606 380
AhdI GACnn_n'nnGTC
Cuts at: 0 1383 1986
Size: 1383 603
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 80 1019 1636 1986
Size: 80 939 617 350
Fragments arranged by size:
939 617 350 80
AlwNI CAG_nnn'CTG
Cuts at: 0 1336 1986
Size: 1336 650
ApaI G_GGCC'C
Cuts at: 0 139 1986
Size: 139 1847
ApoI r'AATT_y
Cuts at: 0 619 1986
Size: 619 1367
AvaI C'yCGr_G
Cuts at: 0 132 927 1079 1270 1894 1986
Size: 132 795 152 191 624 92
Fragments arranged by size:
795 624 191 152 132 92
AvrII C'CTAG_G
Cuts at: 0 139 1986
Size: 139 1847
BamHI G'GATC_C
Cuts at: 0 1173 1986
Size: 1173 813
BanI G'GyrC_C
Cuts at: 0 518 822 1398 1724 1918 1986
Size: 518 304 576 326 194 68
Fragments arranged by size:
576 518 326 304 194 68
BanII G_rGCy'C
Cuts at: 0 139 1086 1986
Size: 139 947 900
Fragments arranged by size:
947 900 139
BbeI G_GCGC'C
Cuts at: 0 826 1986
Size: 826 1160
BbsI GAAGACnn'nnnn_
Cuts at: 0 1469 1906 1986
Size: 1469 437 80
Fragments arranged by size:
1469 437 80
BbvCI CC'TCA_GC
Cuts at: 0 503 1329 1579 1986
Size: 503 826 250 407
Fragments arranged by size:
826 503 407 250
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1662 1696 1986
Size: 1662 34 290
Fragments arranged by size:
1662 290 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1932 1986
Size: 1932 54
BlpI GC'TnA_GC
Cuts at: 0 276 1411 1986
Size: 276 1135 575
Fragments arranged by size:
1135 575 276
Bme1580I G_kGCm'C
Cuts at: 0 139 1401 1735 1923 1986
Size: 139 1262 334 188 63
Fragments arranged by size:
1262 334 188 139 63
BmgBI CAC'GTC
Cuts at: 0 992 1353 1986
Size: 992 361 633
Fragments arranged by size:
992 633 361
BmrI ACTGGGnnnn_n'
Cuts at: 0 1404 1804 1986
Size: 1404 400 182
Fragments arranged by size:
1404 400 182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 684 814 1221 1611 1799 1986
Size: 619 65 130 407 390 188 187
Fragments arranged by size:
619 407 390 188 187 130 65
Bpu10I CC'TnA_GC
Cuts at: 0 241 503 1329 1579 1669 1925 1986
Size: 241 262 826 250 90 256 61
Fragments arranged by size:
826 262 256 250 241 90 61
BsaI GGTCTCn'nnnn_
Cuts at: 0 472 1498 1986
Size: 472 1026 488
Fragments arranged by size:
1026 488 472
BsaHI Gr'CG_yC
Cuts at: 0 823 1427 1688 1986
Size: 823 604 261 298
Fragments arranged by size:
823 604 298 261
BsaWI w'CCGG_w
Cuts at: 0 108 521 1986
Size: 108 413 1465
Fragments arranged by size:
1465 413 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 169 199 839 869 1986
Size: 169 30 640 30 1117
Fragments arranged by size:
1117 640 169 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 413 771 948 1986
Size: 22 391 358 177 1038
Fragments arranged by size:
1038 391 358 177 22
BseYI C'CCAG_C
Cuts at: 0 211 307 529 642 741 788 1336 1601
Size: 211 96 222 113 99 47 548 265
Cuts at: 1601 1986
Size: 385
Fragments arranged by size:
548 385 265 222 211 113 99 96
47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 886 1513 1573 1642 1986
Size: 529 357 627 60 69 344
Fragments arranged by size:
627 529 357 344 69 60
BsiHKAI G_wGCw'C
Cuts at: 0 187 1417 1986
Size: 187 1230 569
Fragments arranged by size:
1230 569 187
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1359 1986
Size: 1359 627
Bsp1286I G_dGCh'C
Cuts at: 0 139 187 1086 1401 1417 1735 1923 1986
Size: 139 48 899 315 16 318 188 63
Fragments arranged by size:
899 318 315 188 139 63 48 16
BspEI T'CCGG_A
Cuts at: 0 108 1986
Size: 108 1878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1153 1545 1614 1986
Size: 1153 392 69 372
Fragments arranged by size:
1153 392 372 69
BsrDI GCAATG_nn'
Cuts at: 0 1226 1329 1986
Size: 1226 103 657
Fragments arranged by size:
1226 657 103
BsrFI r'CCGG_y
Cuts at: 0 271 1986
Size: 271 1715
BsrGI T'GTAC_A
Cuts at: 0 323 1986
Size: 323 1663
BssSI C'ACGA_G
Cuts at: 0 1681 1986
Size: 1681 305
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1491 1986
Size: 1491 495
BstEII G'GTnAC_C
Cuts at: 0 12 384 1986
Size: 12 372 1602
Fragments arranged by size:
1602 372 12
BstYI r'GATC_y
Cuts at: 0 1173 1986
Size: 1173 813
Bsu36I CC'TnA_GG
Cuts at: 0 380 563 716 1913 1986
Size: 380 183 153 1197 73
Fragments arranged by size:
1197 380 183 153 73
BtgI C'CryG_G
Cuts at: 0 538 1986
Size: 538 1448
BtsI GCAGTG_nn'
Cuts at: 0 1804 1986
Size: 1804 182
DraIII CAC_nnn'GTG
Cuts at: 0 1286 1574 1753 1986
Size: 1286 288 179 233
Fragments arranged by size:
1286 288 233 179
DrdI GACnn_nn'nnGTC
Cuts at: 0 372 1986
Size: 372 1614
EaeI y'GGCC_r
Cuts at: 0 452 1072 1986
Size: 452 620 914
Fragments arranged by size:
914 620 452
EarI CTCTTCn'nnn_
Cuts at: 0 40 46 1820 1986
Size: 40 6 1774 166
Fragments arranged by size:
1774 166 40 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1752 1986
Size: 1752 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 684 814 1221 1593 1611 1799 1986
Size: 619 65 130 407 372 18 188 187
Fragments arranged by size:
619 407 372 188 187 130 65 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 354 1840 1986
Size: 354 1486 146
Fragments arranged by size:
1486 354 146
EcoO109I rG'GnC_Cy
Cuts at: 0 96 136 144 202 896 1134 1845 1986
Size: 96 40 8 58 694 238 711 141
Fragments arranged by size:
711 694 238 141 96 58 40 8
EcoRI G'AATT_C
Cuts at: 0 619 1986
Size: 619 1367
EcoRV GAT'ATC
Cuts at: 0 164 1986
Size: 164 1822
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1299 1986
Size: 1299 687
FspI TGC'GCA
Cuts at: 0 1265 1986
Size: 1265 721
HaeII r_GCGC'y
Cuts at: 0 65 255 826 1608 1986
Size: 65 190 571 782 378
Fragments arranged by size:
782 571 378 190 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 109 135 141 173 565 597 839
Size: 103 6 26 6 32 392 32 242
Cuts at: 839 871 906 938 1369 1401 1404 1436 1875
Size: 32 35 32 431 32 3 32 439
Cuts at: 1875 1907 1986
Size: 32 79
Fragments arranged by size:
439 431 392 242 103 79 35 32
32 32 32 32 32 32 26 6
6 3
KasI G'GCGC_C
Cuts at: 0 822 1986
Size: 822 1164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 654 1026 1986
Size: 323 331 372 960
Fragments arranged by size:
960 372 331 323
MscI TGG'CCA
Cuts at: 0 1074 1986
Size: 1074 912
MslI CAynn'nnrTG
Cuts at: 0 439 1618 1986
Size: 439 1179 368
Fragments arranged by size:
1179 439 368
MspA1I CmG'CkG
Cuts at: 0 9 211 462 642 741 792 1199 1336
Size: 9 202 251 180 99 51 407 137
Cuts at: 1336 1451 1740 1986
Size: 115 289 246
Fragments arranged by size:
407 289 251 246 202 180 137 115
99 51 9
NaeI GCC'GGC
Cuts at: 0 273 1986
Size: 273 1713
NarI GG'CG_CC
Cuts at: 0 823 1986
Size: 823 1163
NcoI C'CATG_G
Cuts at: 0 538 1986
Size: 538 1448
NgoMIV G'CCGG_C
Cuts at: 0 271 1986
Size: 271 1715
NspI r_CATG'y
Cuts at: 0 864 1986
Size: 864 1122
PasI CC'CwG_GG
Cuts at: 0 1656 1986
Size: 1656 330
PflMI CCAn_nnn'nTGG
Cuts at: 0 1070 1986
Size: 1070 916
PfoI T'CCnGG_A
Cuts at: 0 661 1820 1986
Size: 661 1159 166
Fragments arranged by size:
1159 661 166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 496 528 1986
Size: 496 32 1458
Fragments arranged by size:
1458 496 32
PpuMI rG'GwC_Cy
Cuts at: 0 96 144 202 896 1986
Size: 96 48 58 694 1090
Fragments arranged by size:
1090 694 96 58 48
PspOMI G'GGCC_C
Cuts at: 0 135 1986
Size: 135 1851
PstI C_TGCA'G
Cuts at: 0 73 293 395 1150 1841 1986
Size: 73 220 102 755 691 145
Fragments arranged by size:
755 691 220 145 102 73
PvuII CAG'CTG
Cuts at: 0 211 462 642 741 792 1199 1336 1986
Size: 211 251 180 99 51 407 137 650
Fragments arranged by size:
650 407 251 211 180 137 99 51
SanDI GG'GwC_CC
Cuts at: 0 202 1986
Size: 202 1784
SbfI CC_TGCA'GG
Cuts at: 0 1841 1986
Size: 1841 145
SexAI A'CCwGG_T
Cuts at: 0 491 1986
Size: 491 1495
SfcI C'TryA_G
Cuts at: 0 69 289 391 850 1146 1194 1837 1986
Size: 69 220 102 459 296 48 643 149
Fragments arranged by size:
643 459 296 220 149 102 69 48
SfoI GGC'GCC
Cuts at: 0 824 1986
Size: 824 1162
SmaI CCC'GGG
Cuts at: 0 1081 1986
Size: 1081 905
SmlI C'TyrA_G
Cuts at: 0 1894 1986
Size: 1894 92
SrfI GCCC'GGGC
Cuts at: 0 1081 1986
Size: 1081 905
StyI C'CwwG_G
Cuts at: 0 139 538 651 884 1692 1701 1952 1986
Size: 139 399 113 233 808 9 251 34
Fragments arranged by size:
808 399 251 233 139 113 34 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 9 330 543 1027 1986
Size: 9 321 213 484 959
Fragments arranged by size:
959 484 321 213 9
TatI w'GTAC_w
Cuts at: 0 323 1986
Size: 323 1663
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 153 185 850 882 1986
Size: 153 32 665 32 1104
Fragments arranged by size:
1104 665 153 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 973 1986
Size: 973 1013
XhoI C'TCGA_G
Cuts at: 0 1894 1986
Size: 1894 92
XmaI C'CCGG_G
Cuts at: 0 1079 1986
Size: 1079 907
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AlfI AlwNI ApaI
ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstEII BstYI Bsu36I BtgI BtsI DraIII
DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SanDI
SbfI SexAI SfcI SfoI SmaI SmlI SrfI StyI
TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AleI AloI
ApaLI AscI AseI AsiSI BaeI BclI BfrBI BglI
BglII BmtI BplI BpuEI BsaAI BsaBI BsiEI BsiWI
BsmI BspHI BsrBI BssHII BstBI BstXI BstZ17I BtgZI
ClaI CspCI DraI EagI EcoICRI FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SspI
StuI SwaI Tth111I XbaI XmnI ZraI