Restriction map for
pFN21AE2038
(Linear) (Six-base) MAPSORT of: KIEE2038.seq Check: 6442 from: 1 to: 1920
KIEE2038.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 127 1920
Size: 127 1793
AccI GT'mk_AC
Cuts at: 0 818 1685 1920
Size: 818 867 235
Fragments arranged by size:
867 818 235
Acc65I G'GTAC_C
Cuts at: 0 605 1920
Size: 605 1315
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 1324 1573 1920
Size: 1066 258 249 347
Fragments arranged by size:
1066 347 258 249
AflIII A'CryG_T
Cuts at: 0 373 886 1920
Size: 373 513 1034
Fragments arranged by size:
1034 513 373
AhdI GACnn_n'nnGTC
Cuts at: 0 1131 1920
Size: 1131 789
AleI CACnn'nnGTG
Cuts at: 0 322 1920
Size: 322 1598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 108 1920
Size: 108 1812
AlwNI CAG_nnn'CTG
Cuts at: 0 70 245 1920
Size: 70 175 1675
Fragments arranged by size:
1675 175 70
ApaLI G'TGCA_C
Cuts at: 0 147 1920
Size: 147 1773
AvaI C'yCGr_G
Cuts at: 0 453 487 838 1098 1151 1920
Size: 453 34 351 260 53 769
Fragments arranged by size:
769 453 351 260 53 34
BamHI G'GATC_C
Cuts at: 0 513 1920
Size: 513 1407
BanI G'GyrC_C
Cuts at: 0 605 1462 1920
Size: 605 857 458
Fragments arranged by size:
857 605 458
BanII G_rGCy'C
Cuts at: 0 222 460 1865 1920
Size: 222 238 1405 55
Fragments arranged by size:
1405 238 222 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 1062 1312 1920
Size: 1062 250 608
Fragments arranged by size:
1062 608 250
BglI GCCn_nnn'nGGC
Cuts at: 0 287 416 1920
Size: 287 129 1504
Fragments arranged by size:
1504 287 129
BlpI GC'TnA_GC
Cuts at: 0 338 349 1920
Size: 338 11 1571
Fragments arranged by size:
1571 338 11
Bme1580I G_kGCm'C
Cuts at: 0 151 488 1465 1920
Size: 151 337 977 455
Fragments arranged by size:
977 455 337 151
BmgBI CAC'GTC
Cuts at: 0 146 324 887 1920
Size: 146 178 563 1033
Fragments arranged by size:
1033 563 178 146
BmrI ACTGGGnnnn_n'
Cuts at: 0 1137 1523 1920
Size: 1137 386 397
Fragments arranged by size:
1137 397 386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 771 1006 1920
Size: 236 535 235 914
Fragments arranged by size:
914 535 236 235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1824 1920
Size: 1824 96
BsaI GGTCTCn'nnnn_
Cuts at: 0 313 637 1920
Size: 313 324 1283
Fragments arranged by size:
1283 324 313
BsaAI yAC'GTr
Cuts at: 0 1458 1920
Size: 1458 462
BsaBI GATnn'nnATC
Cuts at: 0 1237 1920
Size: 1237 683
BsaWI w'CCGG_w
Cuts at: 0 1658 1920
Size: 1658 262
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 214 244 856 871 886 901 1405 1435
Size: 214 30 612 15 15 15 504 30
Cuts at: 1435 1920
Size: 485
Fragments arranged by size:
612 504 485 214 30 30 15 15
15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 907 1413 1920
Size: 907 506 507
Fragments arranged by size:
907 507 506
BseYI C'CCAG_C
Cuts at: 0 868 1029 1146 1920
Size: 868 161 117 774
Fragments arranged by size:
868 774 161 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 300 843 918 1675 1920
Size: 105 195 543 75 757 245
Fragments arranged by size:
757 543 245 195 105 75
BsiEI CG_ry'CG
Cuts at: 0 677 1920
Size: 677 1243
BsiHKAI G_wGCw'C
Cuts at: 0 97 151 222 451 992 1099 1335 1920
Size: 97 54 71 229 541 107 236 585
Fragments arranged by size:
585 541 236 229 107 97 71 54
Bsp1286I G_dGCh'C
Cuts at: 0 97 151 222 451 460 488 992 1099
Size: 97 54 71 229 9 28 504 107
Cuts at: 1099 1335 1465 1865 1920
Size: 236 130 400 55
Fragments arranged by size:
504 400 236 229 130 107 97 71
55 54 28 9
BspHI T'CATG_A
Cuts at: 0 1505 1920
Size: 1505 415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 127 193 602 655 1697 1750 1920
Size: 127 66 409 53 1042 53 170
Fragments arranged by size:
1042 409 170 127 66 53 53
BsrBI CCG'CTC
Cuts at: 0 755 1920
Size: 755 1165
BsrDI GCAATG_nn'
Cuts at: 0 117 1542 1920
Size: 117 1425 378
Fragments arranged by size:
1425 378 117
BsrFI r'CCGG_y
Cuts at: 0 169 802 1091 1357 1920
Size: 169 633 289 266 563
Fragments arranged by size:
633 563 289 266 169
BssHII G'CGCG_C
Cuts at: 0 631 743 1920
Size: 631 112 1177
Fragments arranged by size:
1177 631 112
BssSI C'ACGA_G
Cuts at: 0 502 1920
Size: 502 1418
BstAPI GCAn_nnn'nTGC
Cuts at: 0 740 1920
Size: 740 1180
BstXI CCAn_nnnn'nTGG
Cuts at: 0 876 1920
Size: 876 1044
BstYI r'GATC_y
Cuts at: 0 513 1920
Size: 513 1407
BtgI C'CryG_G
Cuts at: 0 413 875 1541 1920
Size: 413 462 666 379
Fragments arranged by size:
666 462 413 379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 188 1920
Size: 188 1732
BtsI GCAGTG_nn'
Cuts at: 0 354 759 939 1498 1920
Size: 354 405 180 559 422
Fragments arranged by size:
559 422 405 354 180
DrdI GACnn_nn'nnGTC
Cuts at: 0 1294 1920
Size: 1294 626
EaeI y'GGCC_r
Cuts at: 0 368 638 674 756 1895 1920
Size: 368 270 36 82 1139 25
Fragments arranged by size:
1139 368 270 82 36 25
EagI C'GGCC_G
Cuts at: 0 674 1920
Size: 674 1246
EarI CTCTTCn'nnn_
Cuts at: 0 1042 1390 1920
Size: 1042 348 530
Fragments arranged by size:
1042 530 348
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 222 283 1920
Size: 222 61 1637
Fragments arranged by size:
1637 222 61
EcoICRI GAG'CTC
Cuts at: 0 220 1920
Size: 220 1700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 771 1006 1066 1324 1573 1920
Size: 236 535 235 60 258 249 347
Fragments arranged by size:
535 347 258 249 236 235 60
EcoO109I rG'GnC_Cy
Cuts at: 0 1804 1920
Size: 1804 116
EcoRV GAT'ATC
Cuts at: 0 975 1920
Size: 975 945
HaeII r_GCGC'y
Cuts at: 0 835 1384 1920
Size: 835 549 536
Fragments arranged by size:
835 549 536
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 856 888 904 936 1117 1149 1631 1663
Size: 856 32 16 32 181 32 482 32
Cuts at: 1663 1920
Size: 257
Fragments arranged by size:
856 482 257 181 32 32 32 32
16
HincII GTy'rAC
Cuts at: 0 1178 1920
Size: 1178 742
KpnI G_GTAC'C
Cuts at: 0 609 1920
Size: 609 1311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 1451 1920
Size: 455 996 469
Fragments arranged by size:
996 469 455
MscI TGG'CCA
Cuts at: 0 370 1920
Size: 370 1550
MslI CAynn'nnrTG
Cuts at: 0 110 322 1021 1345 1920
Size: 110 212 699 324 575
Fragments arranged by size:
699 575 324 212 110
MspA1I CmG'CkG
Cuts at: 0 245 415 1044 1920
Size: 245 170 629 876
Fragments arranged by size:
876 629 245 170
NaeI GCC'GGC
Cuts at: 0 804 1920
Size: 804 1116
NgoMIV G'CCGG_C
Cuts at: 0 802 1920
Size: 802 1118
NspI r_CATG'y
Cuts at: 0 377 1274 1365 1920
Size: 377 897 91 555
Fragments arranged by size:
897 555 377 91
PasI CC'CwG_GG
Cuts at: 0 1632 1920
Size: 1632 288
PciI A'CATG_T
Cuts at: 0 373 1920
Size: 373 1547
PflMI CCAn_nnn'nTGG
Cuts at: 0 366 1611 1920
Size: 366 1245 309
Fragments arranged by size:
1245 366 309
PfoI T'CCnGG_A
Cuts at: 0 213 983 1568 1920
Size: 213 770 585 352
Fragments arranged by size:
770 585 352 213
PpuMI rG'GwC_Cy
Cuts at: 0 1804 1920
Size: 1804 116
PstI C_TGCA'G
Cuts at: 0 66 1920
Size: 66 1854
PvuII CAG'CTG
Cuts at: 0 245 1044 1920
Size: 245 799 876
Fragments arranged by size:
876 799 245
SacI G_AGCT'C
Cuts at: 0 222 1920
Size: 222 1698
SacII CC_GC'GG
Cuts at: 0 416 1920
Size: 416 1504
SanDI GG'GwC_CC
Cuts at: 0 1804 1920
Size: 1804 116
SapI GCTCTTCn'nnn_
Cuts at: 0 1390 1920
Size: 1390 530
ScaI AGT'ACT
Cuts at: 0 547 1558 1920
Size: 547 1011 362
Fragments arranged by size:
1011 547 362
SfcI C'TryA_G
Cuts at: 0 62 1136 1315 1920
Size: 62 1074 179 605
Fragments arranged by size:
1074 605 179 62
SgrAI Cr'CCGG_yG
Cuts at: 0 169 1920
Size: 169 1751
SmaI CCC'GGG
Cuts at: 0 455 1153 1920
Size: 455 698 767
Fragments arranged by size:
767 698 455
SmlI C'TyrA_G
Cuts at: 0 1839 1920
Size: 1839 81
SphI G_CATG'C
Cuts at: 0 1274 1920
Size: 1274 646
StuI AGG'CCT
Cuts at: 0 739 796 1920
Size: 739 57 1124
Fragments arranged by size:
1124 739 57
StyI C'CwwG_G
Cuts at: 0 1416 1920
Size: 1416 504
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1119 1542 1920
Size: 1119 423 378
Fragments arranged by size:
1119 423 378
TatI w'GTAC_w
Cuts at: 0 545 1556 1920
Size: 545 1011 364
Fragments arranged by size:
1011 545 364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 214 246 871 903 1920
Size: 214 32 625 32 1017
Fragments arranged by size:
1017 625 214 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1066 1434 1681 1920
Size: 1066 368 247 239
Fragments arranged by size:
1066 368 247 239
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 17 1920
Size: 17 1903
XmaI C'CCGG_G
Cuts at: 0 453 1151 1920
Size: 453 698 769
Fragments arranged by size:
769 698 453
XmnI GAAnn'nnTTC
Cuts at: 0 1558 1920
Size: 1558 362
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AleI AlfI
AlwNI ApaLI AvaI BamHI BanI BanII BbsI BglI
BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstXI BstYI BtgI BtgZI BtsI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV HaeII
Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI
NgoMIV NspI PasI PciI PflMI PfoI PpuMI PstI
PvuII SacI SacII SanDI SapI ScaI SfcI SgrAI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AloI ApaI ApoI
AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BmtI BplI Bpu10I BsaHI
BsiWI BsmI BsmBI BspEI BsrGI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraI DraIII EcoNI EcoRI FalI
FseI FspI FspAI HindIII HpaI KasI MfeI MluI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SalI SbfI SexAI SfiI SfoI SgfI
SnaBI SpeI SrfI SspI SwaI XbaI XhoI ZraI