Restriction map for
pFN21AE2030
(Linear) (Six-base) MAPSORT of: KIEE2030.seq Check: 8019 from: 1 to: 1860
KIEE2030.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AccI GT'mk_AC
Cuts at: 0 482 1860
Size: 482 1378
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1155 1610 1860
Size: 1155 455 250
Fragments arranged by size:
1155 455 250
AleI CACnn'nnGTG
Cuts at: 0 1683 1860
Size: 1683 177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 249 1860
Size: 249 1611
AlwNI CAG_nnn'CTG
Cuts at: 0 359 1413 1860
Size: 359 1054 447
Fragments arranged by size:
1054 447 359
ApoI r'AATT_y
Cuts at: 0 1699 1860
Size: 1699 161
BanI G'GyrC_C
Cuts at: 0 233 1860
Size: 233 1627
BanII G_rGCy'C
Cuts at: 0 293 1860
Size: 293 1567
BbsI GAAGACnn'nnnn_
Cuts at: 0 368 391 1205 1663 1860
Size: 368 23 814 458 197
Fragments arranged by size:
814 458 368 197 23
BbvCI CC'TCA_GC
Cuts at: 0 1385 1860
Size: 1385 475
BfrBI ATG'CAT
Cuts at: 0 1215 1860
Size: 1215 645
BmrI ACTGGGnnnn_n'
Cuts at: 0 369 1860
Size: 369 1491
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 1751 1860
Size: 336 1415 109
Fragments arranged by size:
1415 336 109
Bpu10I CC'TnA_GC
Cuts at: 0 568 1385 1860
Size: 568 817 475
Fragments arranged by size:
817 568 475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1740 1860
Size: 1006 734 120
Fragments arranged by size:
1006 734 120
BsaBI GATnn'nnATC
Cuts at: 0 881 1860
Size: 881 979
BsaWI w'CCGG_w
Cuts at: 0 1194 1860
Size: 1194 666
BsiHKAI G_wGCw'C
Cuts at: 0 66 257 1049 1860
Size: 66 191 792 811
Fragments arranged by size:
811 792 191 66
Bsp1286I G_dGCh'C
Cuts at: 0 66 257 293 1049 1860
Size: 66 191 36 756 811
Fragments arranged by size:
811 756 191 66 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1011 1860
Size: 1011 849
BsrBI CCG'CTC
Cuts at: 0 12 1860
Size: 12 1848
BsrDI GCAATG_nn'
Cuts at: 0 1187 1560 1860
Size: 1187 373 300
Fragments arranged by size:
1187 373 300
BsrGI T'GTAC_A
Cuts at: 0 807 1574 1860
Size: 807 767 286
Fragments arranged by size:
807 767 286
BssSI C'ACGA_G
Cuts at: 0 98 1860
Size: 98 1762
BstYI r'GATC_y
Cuts at: 0 345 780 1860
Size: 345 435 1080
Fragments arranged by size:
1080 435 345
BtgI C'CryG_G
Cuts at: 0 1684 1860
Size: 1684 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1072 1860
Size: 1072 788
BtsI GCAGTG_nn'
Cuts at: 0 917 1860
Size: 917 943
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 966 1001 1860
Size: 966 35 859
Fragments arranged by size:
966 859 35
DraI TTT'AAA
Cuts at: 0 741 1860
Size: 741 1119
DrdI GACnn_nn'nnGTC
Cuts at: 0 1842 1860
Size: 1842 18
EarI CTCTTCn'nnn_
Cuts at: 0 281 1860
Size: 281 1579
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 1155 1610 1751 1860
Size: 336 819 455 141 109
Fragments arranged by size:
819 455 336 141 109
EcoO109I rG'GnC_Cy
Cuts at: 0 264 1860
Size: 264 1596
EcoRV GAT'ATC
Cuts at: 0 964 1860
Size: 964 896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 568 1860
Size: 568 1292
HincII GTy'rAC
Cuts at: 0 1323 1860
Size: 1323 537
HindIII A'AGCT_T
Cuts at: 0 464 472 754 1592 1860
Size: 464 8 282 838 268
Fragments arranged by size:
838 464 282 268 8
MfeI C'AATT_G
Cuts at: 0 1301 1860
Size: 1301 559
MslI CAynn'nnrTG
Cuts at: 0 944 1570 1683 1860
Size: 944 626 113 177
Fragments arranged by size:
944 626 177 113
MspA1I CmG'CkG
Cuts at: 0 1086 1860
Size: 1086 774
NsiI A_TGCA'T
Cuts at: 0 1217 1860
Size: 1217 643
NspI r_CATG'y
Cuts at: 0 215 525 955 1360 1860
Size: 215 310 430 405 500
Fragments arranged by size:
500 430 405 310 215
PflMI CCAn_nnn'nTGG
Cuts at: 0 359 1059 1860
Size: 359 700 801
Fragments arranged by size:
801 700 359
PsiI TTA'TAA
Cuts at: 0 728 1860
Size: 728 1132
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 429 461 1860
Size: 429 32 1399
Fragments arranged by size:
1399 429 32
PstI C_TGCA'G
Cuts at: 0 1366 1725 1860
Size: 1366 359 135
Fragments arranged by size:
1366 359 135
PvuII CAG'CTG
Cuts at: 0 1086 1860
Size: 1086 774
SapI GCTCTTCn'nnn_
Cuts at: 0 281 1860
Size: 281 1579
SfcI C'TryA_G
Cuts at: 0 450 668 764 1362 1721 1860
Size: 450 218 96 598 359 139
Fragments arranged by size:
598 450 359 218 139 96
SmlI C'TyrA_G
Cuts at: 0 985 1755 1860
Size: 985 770 105
Fragments arranged by size:
985 770 105
StuI AGG'CCT
Cuts at: 0 1390 1860
Size: 1390 470
StyI C'CwwG_G
Cuts at: 0 1391 1515 1784 1860
Size: 1391 124 269 76
Fragments arranged by size:
1391 269 124 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 170 925 1860
Size: 170 755 935
Fragments arranged by size:
935 755 170
TatI w'GTAC_w
Cuts at: 0 807 1574 1860
Size: 807 767 286
Fragments arranged by size:
807 767 286
XbaI T'CTAG_A
Cuts at: 0 426 1860
Size: 426 1434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1513 1782 1860
Size: 1513 269 78
Fragments arranged by size:
1513 269 78
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI ApoI BanI BanII
BbsI BbvCI BfrBI BmrI BpmI Bpu10I BpuEI BsaBI
BsaWI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrGI BssSI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EarI
Eco57MI EcoO109I EcoRV FalI HincII HindIII MfeI MslI
MspA1I NsiI NspI PflMI PsiI PsrI PstI PvuII
SapI SfcI SmlI StuI StyI TaqII TatI XbaI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrFI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I ClaI DraIII EaeI EagI EciI
EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII Hin4I
HpaI KasI KpnI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XhoI
XmaI XmnI ZraI