Restriction map for
pFN21AE2027
(Linear) (Six-base) MAPSORT of: KIEE2027.seq Check: 3810 from: 1 to: 1830
KIEE2027.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 489 582 835 1359 1583 1830
Size: 489 93 253 524 224 247
Fragments arranged by size:
524 489 253 247 224 93
AflIII A'CryG_T
Cuts at: 0 892 1270 1597 1830
Size: 892 378 327 233
Fragments arranged by size:
892 378 327 233
AleI CACnn'nnGTG
Cuts at: 0 34 1830
Size: 34 1796
AlwNI CAG_nnn'CTG
Cuts at: 0 1778 1830
Size: 1778 52
ApaLI G'TGCA_C
Cuts at: 0 1098 1422 1830
Size: 1098 324 408
Fragments arranged by size:
1098 408 324
ApoI r'AATT_y
Cuts at: 0 1348 1759 1830
Size: 1348 411 71
Fragments arranged by size:
1348 411 71
AvaI C'yCGr_G
Cuts at: 0 1117 1830
Size: 1117 713
BamHI G'GATC_C
Cuts at: 0 1216 1830
Size: 1216 614
BanII G_rGCy'C
Cuts at: 0 294 843 1529 1830
Size: 294 549 686 301
Fragments arranged by size:
686 549 301 294
BbsI GAAGACnn'nnnn_
Cuts at: 0 1132 1830
Size: 1132 698
BglII A'GATC_T
Cuts at: 0 338 1830
Size: 338 1492
Bme1580I G_kGCm'C
Cuts at: 0 1102 1426 1830
Size: 1102 324 404
Fragments arranged by size:
1102 404 324
BmrI ACTGGGnnnn_n'
Cuts at: 0 297 598 1072 1830
Size: 297 301 474 758
Fragments arranged by size:
758 474 301 297
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1346 1830
Size: 1346 484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 701 986 1830
Size: 278 423 285 844
Fragments arranged by size:
844 423 285 278
Bpu10I CC'TnA_GC
Cuts at: 0 557 1830
Size: 557 1273
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1037 1438 1830
Size: 499 538 401 392
Fragments arranged by size:
538 499 401 392
BsaI GGTCTCn'nnnn_
Cuts at: 0 460 652 1830
Size: 460 192 1178
Fragments arranged by size:
1178 460 192
BsaBI GATnn'nnATC
Cuts at: 0 217 343 1830
Size: 217 126 1487
Fragments arranged by size:
1487 217 126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 61 91 163 193 1516 1546 1685 1706
Size: 61 30 72 30 1323 30 139 21
Cuts at: 1706 1715 1736 1830
Size: 9 21 94
Fragments arranged by size:
1323 139 94 72 61 30 30 30
21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1399 1714 1830
Size: 1399 315 116
Fragments arranged by size:
1399 315 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 408 969 1830
Size: 408 561 861
Fragments arranged by size:
861 561 408
BsiHKAI G_wGCw'C
Cuts at: 0 696 1102 1332 1426 1830
Size: 696 406 230 94 404
Fragments arranged by size:
696 406 404 230 94
BsmI GAATG_Cn'
Cuts at: 0 1048 1830
Size: 1048 782
Bsp1286I G_dGCh'C
Cuts at: 0 294 696 843 1102 1332 1426 1529 1830
Size: 294 402 147 259 230 94 103 301
Fragments arranged by size:
402 301 294 259 230 147 103 94
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 650 1830
Size: 650 1180
BsrDI GCAATG_nn'
Cuts at: 0 717 1830
Size: 717 1113
BsrGI T'GTAC_A
Cuts at: 0 538 802 1830
Size: 538 264 1028
Fragments arranged by size:
1028 538 264
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1544 1830
Size: 1544 286
BstEII G'GTnAC_C
Cuts at: 0 637 1830
Size: 637 1193
BstXI CCAn_nnnn'nTGG
Cuts at: 0 775 1830
Size: 775 1055
BstYI r'GATC_y
Cuts at: 0 338 1216 1830
Size: 338 878 614
Fragments arranged by size:
878 614 338
BtgI C'CryG_G
Cuts at: 0 80 450 787 1830
Size: 80 370 337 1043
Fragments arranged by size:
1043 370 337 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1304 1830
Size: 1304 526
BtsI GCAGTG_nn'
Cuts at: 0 450 673 1066 1524 1830
Size: 450 223 393 458 306
Fragments arranged by size:
458 450 393 306 223
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1042 1077 1830
Size: 1042 35 753
Fragments arranged by size:
1042 753 35
DraIII CAC_nnn'GTG
Cuts at: 0 1544 1830
Size: 1544 286
EaeI y'GGCC_r
Cuts at: 0 447 1830
Size: 447 1383
EarI CTCTTCn'nnn_
Cuts at: 0 155 1442 1830
Size: 155 1287 388
Fragments arranged by size:
1287 388 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 910 1830
Size: 910 920
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 489 582 701 835 986 1359 1583
Size: 278 211 93 119 134 151 373 224
Cuts at: 1583 1830
Size: 247
Fragments arranged by size:
373 278 247 224 211 151 134 119
93
EcoRI G'AATT_C
Cuts at: 0 1348 1830
Size: 1348 482
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1411 1443 1559 1591 1685 1717 1830
Size: 1411 32 116 32 94 32 113
Fragments arranged by size:
1411 116 113 94 32 32 32
HincII GTy'rAC
Cuts at: 0 231 1830
Size: 231 1599
HindIII A'AGCT_T
Cuts at: 0 400 780 1088 1830
Size: 400 380 308 742
Fragments arranged by size:
742 400 380 308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1136 1682 1830
Size: 1136 546 148
Fragments arranged by size:
1136 546 148
MscI TGG'CCA
Cuts at: 0 449 1830
Size: 449 1381
MslI CAynn'nnrTG
Cuts at: 0 34 1830
Size: 34 1796
MspA1I CmG'CkG
Cuts at: 0 421 626 1178 1379 1778 1830
Size: 421 205 552 201 399 52
Fragments arranged by size:
552 421 399 205 201 52
NcoI C'CATG_G
Cuts at: 0 787 1830
Size: 787 1043
NspI r_CATG'y
Cuts at: 0 372 911 1274 1601 1830
Size: 372 539 363 327 229
Fragments arranged by size:
539 372 363 327 229
PciI A'CATG_T
Cuts at: 0 1270 1597 1830
Size: 1270 327 233
Fragments arranged by size:
1270 327 233
PflMI CCAn_nnn'nTGG
Cuts at: 0 287 748 1830
Size: 287 461 1082
Fragments arranged by size:
1082 461 287
PfoI T'CCnGG_A
Cuts at: 0 1494 1830
Size: 1494 336
PshAI GACnn'nnGTC
Cuts at: 0 1617 1830
Size: 1617 213
PstI C_TGCA'G
Cuts at: 0 445 1519 1590 1830
Size: 445 1074 71 240
Fragments arranged by size:
1074 445 240 71
PvuII CAG'CTG
Cuts at: 0 421 626 1178 1379 1778 1830
Size: 421 205 552 201 399 52
Fragments arranged by size:
552 421 399 205 201 52
SexAI A'CCwGG_T
Cuts at: 0 137 1830
Size: 137 1693
SfcI C'TryA_G
Cuts at: 0 441 1515 1586 1830
Size: 441 1074 71 244
Fragments arranged by size:
1074 441 244 71
SmlI C'TyrA_G
Cuts at: 0 514 1052 1417 1830
Size: 514 538 365 413
Fragments arranged by size:
538 514 413 365
StyI C'CwwG_G
Cuts at: 0 787 1667 1830
Size: 787 880 163
Fragments arranged by size:
880 787 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 599 1830
Size: 599 1231
TatI w'GTAC_w
Cuts at: 0 538 802 1830
Size: 538 264 1028
Fragments arranged by size:
1028 538 264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1058 1090 1706 1738 1830
Size: 1058 32 616 32 92
Fragments arranged by size:
1058 616 92 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 206 753 931 961 1665 1724 1830
Size: 206 547 178 30 704 59 106
Fragments arranged by size:
704 547 206 178 106 59 30
XmnI GAAnn'nnTTC
Cuts at: 0 1451 1830
Size: 1451 379
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaLI ApoI AvaI BamHI
BanII BbsI BglII Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaXI BseRI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BsrGI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI DraIII EaeI EarI EciI
Eco57MI EcoRI Hin4I HincII HindIII MmeI MscI MslI
MspA1I NcoI NspI PciI PflMI PfoI PshAI PstI
PvuII SexAI SfcI SmlI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmtI BsaAI BsaHI BsaWI BseYI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII
BssSI BstBI BstZ17I Bsu36I ClaI DraI DrdI EagI
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmaI ZraI