Restriction map for
pFN21AE2024
(Linear) (Six-base) MAPSORT of: KIEE2024.seq Check: 7536 from: 1 to: 1812
KIEE2024.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1698 1812
Size: 1698 114
AclI AA'CG_TT
Cuts at: 0 223 1812
Size: 223 1589
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 1812
Size: 273 1539
AflIII A'CryG_T
Cuts at: 0 1003 1812
Size: 1003 809
AhdI GACnn_n'nnGTC
Cuts at: 0 538 1812
Size: 538 1274
AleI CACnn'nnGTG
Cuts at: 0 1649 1812
Size: 1649 163
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 425 457 1812
Size: 425 32 1355
Fragments arranged by size:
1355 425 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1583 1662 1812
Size: 1583 79 150
Fragments arranged by size:
1583 150 79
ApoI r'AATT_y
Cuts at: 0 490 1291 1812
Size: 490 801 521
Fragments arranged by size:
801 521 490
AvaI C'yCGr_G
Cuts at: 0 424 1776 1812
Size: 424 1352 36
Fragments arranged by size:
1352 424 36
BamHI G'GATC_C
Cuts at: 0 1175 1812
Size: 1175 637
BanI G'GyrC_C
Cuts at: 0 265 355 1812
Size: 265 90 1457
Fragments arranged by size:
1457 265 90
BanII G_rGCy'C
Cuts at: 0 195 587 1735 1812
Size: 195 392 1148 77
Fragments arranged by size:
1148 392 195 77
BbeI G_GCGC'C
Cuts at: 0 269 1812
Size: 269 1543
BbsI GAAGACnn'nnnn_
Cuts at: 0 142 1812
Size: 142 1670
BbvCI CC'TCA_GC
Cuts at: 0 890 1346 1812
Size: 890 456 466
Fragments arranged by size:
890 466 456
BciVI GTATCCnnnnn_n'
Cuts at: 0 1309 1812
Size: 1309 503
BclI T'GATC_A
Cuts at: 0 1023 1433 1812
Size: 1023 410 379
Fragments arranged by size:
1023 410 379
BglII A'GATC_T
Cuts at: 0 1331 1812
Size: 1331 481
BmgBI CAC'GTC
Cuts at: 0 1006 1812
Size: 1006 806
BmrI ACTGGGnnnn_n'
Cuts at: 0 618 1261 1638 1812
Size: 618 643 377 174
Fragments arranged by size:
643 618 377 174
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 120 263 746 1812
Size: 120 143 483 1066
Fragments arranged by size:
1066 483 143 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 716 1812
Size: 716 1096
Bpu10I CC'TnA_GC
Cuts at: 0 890 1346 1812
Size: 890 456 466
Fragments arranged by size:
890 466 456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 1812
Size: 170 1642
BsaI GGTCTCn'nnnn_
Cuts at: 0 525 1812
Size: 525 1287
BsaAI yAC'GTr
Cuts at: 0 1320 1812
Size: 1320 492
BsaHI Gr'CG_yC
Cuts at: 0 266 1193 1812
Size: 266 927 619
Fragments arranged by size:
927 619 266
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 425 455 867 897 922 952 1812
Size: 425 30 412 30 25 30 860
Fragments arranged by size:
860 425 412 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 146 306 309 577 675 1342 1363 1692
Size: 146 160 3 268 98 667 21 329
Cuts at: 1692 1812
Size: 120
Fragments arranged by size:
667 329 268 160 146 120 98 21
3
BseYI C'CCAG_C
Cuts at: 0 345 635 952 1670 1726 1812
Size: 345 290 317 718 56 86
Fragments arranged by size:
718 345 317 290 86 56
BsiEI CG_ry'CG
Cuts at: 0 1469 1812
Size: 1469 343
BsiHKAI G_wGCw'C
Cuts at: 0 33 195 587 1812
Size: 33 162 392 1225
Fragments arranged by size:
1225 392 162 33
Bsp1286I G_dGCh'C
Cuts at: 0 33 195 587 1735 1812
Size: 33 162 392 1148 77
Fragments arranged by size:
1148 392 162 77 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1582 1698 1812
Size: 1582 116 114
Fragments arranged by size:
1582 116 114
BsrBI CCG'CTC
Cuts at: 0 1416 1812
Size: 1416 396
BsrDI GCAATG_nn'
Cuts at: 0 300 417 917 1812
Size: 300 117 500 895
Fragments arranged by size:
895 500 300 117
BssSI C'ACGA_G
Cuts at: 0 237 1812
Size: 237 1575
BstXI CCAn_nnnn'nTGG
Cuts at: 0 859 1812
Size: 859 953
BstYI r'GATC_y
Cuts at: 0 1175 1331 1812
Size: 1175 156 481
Fragments arranged by size:
1175 481 156
Bsu36I CC'TnA_GG
Cuts at: 0 967 1812
Size: 967 845
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1393 1812
Size: 1393 419
BtsI GCAGTG_nn'
Cuts at: 0 591 1584 1812
Size: 591 993 228
Fragments arranged by size:
993 591 228
EaeI y'GGCC_r
Cuts at: 0 605 914 1554 1812
Size: 605 309 640 258
Fragments arranged by size:
640 605 309 258
EarI CTCTTCn'nnn_
Cuts at: 0 312 715 1049 1812
Size: 312 403 334 763
Fragments arranged by size:
763 403 334 312
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1426 1507 1812
Size: 1426 81 305
Fragments arranged by size:
1426 305 81
EcoICRI GAG'CTC
Cuts at: 0 193 585 1812
Size: 193 392 1227
Fragments arranged by size:
1227 392 193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 716 1812
Size: 273 443 1096
Fragments arranged by size:
1096 443 273
EcoO109I rG'GnC_Cy
Cuts at: 0 963 1812
Size: 963 849
EcoRI G'AATT_C
Cuts at: 0 1291 1812
Size: 1291 521
EcoRV GAT'ATC
Cuts at: 0 5 1812
Size: 5 1807
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 38 579 1812
Size: 38 541 1233
Fragments arranged by size:
1233 541 38
FspI TGC'GCA
Cuts at: 0 597 1812
Size: 597 1215
HaeII r_GCGC'y
Cuts at: 0 269 1374 1812
Size: 269 1105 438
Fragments arranged by size:
1105 438 269
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 524 556 1791 1812
Size: 524 32 1235 21
Fragments arranged by size:
1235 524 32 21
HincII GTy'rAC
Cuts at: 0 227 681 1257 1769 1812
Size: 227 454 576 512 43
Fragments arranged by size:
576 512 454 227 43
HpaI GTT'AAC
Cuts at: 0 227 1812
Size: 227 1585
KasI G'GCGC_C
Cuts at: 0 265 1812
Size: 265 1547
MfeI C'AATT_G
Cuts at: 0 482 1812
Size: 482 1330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1812
Size: 184 1628
MscI TGG'CCA
Cuts at: 0 607 916 1812
Size: 607 309 896
Fragments arranged by size:
896 607 309
MslI CAynn'nnrTG
Cuts at: 0 1649 1812
Size: 1649 163
MspA1I CmG'CkG
Cuts at: 0 339 648 822 1721 1812
Size: 339 309 174 899 91
Fragments arranged by size:
899 339 309 174 91
NarI GG'CG_CC
Cuts at: 0 266 1812
Size: 266 1546
PflMI CCAn_nnn'nTGG
Cuts at: 0 283 352 1812
Size: 283 69 1460
Fragments arranged by size:
1460 283 69
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 425 457 1812
Size: 425 32 1355
Fragments arranged by size:
1355 425 32
PpuMI rG'GwC_Cy
Cuts at: 0 963 1812
Size: 963 849
PstI C_TGCA'G
Cuts at: 0 401 578 1394 1579 1667 1695 1812
Size: 401 177 816 185 88 28 117
Fragments arranged by size:
816 401 185 177 117 88 28
PvuII CAG'CTG
Cuts at: 0 339 648 1812
Size: 339 309 1164
Fragments arranged by size:
1164 339 309
SacI G_AGCT'C
Cuts at: 0 195 587 1812
Size: 195 392 1225
Fragments arranged by size:
1225 392 195
SbfI CC_TGCA'GG
Cuts at: 0 1667 1812
Size: 1667 145
SfcI C'TryA_G
Cuts at: 0 251 397 574 930 1390 1575 1663 1691
Size: 251 146 177 356 460 185 88 28
Cuts at: 1691 1812
Size: 121
Fragments arranged by size:
460 356 251 185 177 146 121 88
28
SfoI GGC'GCC
Cuts at: 0 267 1812
Size: 267 1545
SmaI CCC'GGG
Cuts at: 0 1778 1812
Size: 1778 34
SmlI C'TyrA_G
Cuts at: 0 185 1812
Size: 185 1627
SpeI A'CTAG_T
Cuts at: 0 462 1812
Size: 462 1350
SspI AAT'ATT
Cuts at: 0 79 1812
Size: 79 1733
StuI AGG'CCT
Cuts at: 0 1305 1812
Size: 1305 507
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 294 1812
Size: 294 1518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1331 1648 1812
Size: 1331 317 164
Fragments arranged by size:
1331 317 164
XmaI C'CCGG_G
Cuts at: 0 1776 1812
Size: 1776 36
XmnI GAAnn'nnTTC
Cuts at: 0 204 382 440 491 1295 1785 1812
Size: 204 178 58 51 804 490 27
Fragments arranged by size:
804 490 204 178 58 51 27
Enzymes that do cut:
AarI AclI AcuI AflIII AhdI AleI AloI AlwNI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglII BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BssSI BstXI BstYI
Bsu36I BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HpaI KasI MfeI MmeI MscI MslI MspA1I NarI
PflMI PpiI PpuMI PstI PvuII SacI SbfI SfcI
SfoI SmaI SmlI SpeI SspI StuI TaqII XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AlfI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BcgI BfrBI
BglI BlpI Bme1580I BmtI BsaBI BsaWI BsgI BsiWI
BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I BtgI ClaI CspCI DraI DraIII
DrdI EagI EcoNI FseI FspAI HindIII KpnI MluI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI
SphI SrfI StyI SwaI TatI TstI Tth111I XbaI
XhoI ZraI