Restriction map for
pFN21AE2022
(Linear) (Six-base) MAPSORT of: KIEE2022.seq Check: 9194 from: 1 to: 1797
KIEE2022.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 218 1797
Size: 218 1579
AccI GT'mk_AC
Cuts at: 0 378 1797
Size: 378 1419
Acc65I G'GTAC_C
Cuts at: 0 251 1797
Size: 251 1546
AlwNI CAG_nnn'CTG
Cuts at: 0 907 1151 1797
Size: 907 244 646
Fragments arranged by size:
907 646 244
ApoI r'AATT_y
Cuts at: 0 121 1797
Size: 121 1676
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 309 342 1797
Size: 309 33 1455
Fragments arranged by size:
1455 309 33
BamHI G'GATC_C
Cuts at: 0 762 1797
Size: 762 1035
BanI G'GyrC_C
Cuts at: 0 251 1096 1797
Size: 251 845 701
Fragments arranged by size:
845 701 251
BanII G_rGCy'C
Cuts at: 0 400 1255 1447 1780 1797
Size: 400 855 192 333 17
Fragments arranged by size:
855 400 333 192 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 1400 1797
Size: 1400 397
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 597 631 1797
Size: 597 34 1166
Fragments arranged by size:
1166 597 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 672 1245 1797
Size: 672 573 552
Fragments arranged by size:
672 573 552
BclI T'GATC_A
Cuts at: 0 1509 1797
Size: 1509 288
BfrBI ATG'CAT
Cuts at: 0 457 1797
Size: 457 1340
BglI GCCn_nnn'nGGC
Cuts at: 0 1123 1797
Size: 1123 674
Bme1580I G_kGCm'C
Cuts at: 0 652 1137 1797
Size: 652 485 660
Fragments arranged by size:
660 652 485
BmrI ACTGGGnnnn_n'
Cuts at: 0 521 1035 1460 1778 1797
Size: 521 514 425 318 19
Fragments arranged by size:
521 514 425 318 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1093 1797
Size: 1093 704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1358 1797
Size: 1358 439
Bpu10I CC'TnA_GC
Cuts at: 0 203 1303 1797
Size: 203 1100 494
Fragments arranged by size:
1100 494 203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1461 1797
Size: 1461 336
BsaAI yAC'GTr
Cuts at: 0 1703 1797
Size: 1703 94
BsaWI w'CCGG_w
Cuts at: 0 1175 1797
Size: 1175 622
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 580 1082 1129 1394 1788 1797
Size: 580 502 47 265 394 9
Fragments arranged by size:
580 502 394 265 47 9
BseYI C'CCAG_C
Cuts at: 0 1781 1797
Size: 1781 16
BsiHKAI G_wGCw'C
Cuts at: 0 209 508 1079 1255 1780 1797
Size: 209 299 571 176 525 17
Fragments arranged by size:
571 525 299 209 176 17
Bsp1286I G_dGCh'C
Cuts at: 0 209 400 508 652 1079 1137 1255 1447
Size: 209 191 108 144 427 58 118 192
Cuts at: 1447 1780 1797
Size: 333 17
Fragments arranged by size:
427 333 209 192 191 144 118 108
58 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 218 1797
Size: 218 1579
BsrGI T'GTAC_A
Cuts at: 0 1082 1797
Size: 1082 715
BssSI C'ACGA_G
Cuts at: 0 1576 1797
Size: 1576 221
BstBI TT'CG_AA
Cuts at: 0 265 1797
Size: 265 1532
BstEII G'GTnAC_C
Cuts at: 0 564 1797
Size: 564 1233
BstYI r'GATC_y
Cuts at: 0 273 762 1797
Size: 273 489 1035
Fragments arranged by size:
1035 489 273
Bsu36I CC'TnA_GG
Cuts at: 0 908 1787 1797
Size: 908 879 10
Fragments arranged by size:
908 879 10
BtsI GCAGTG_nn'
Cuts at: 0 1496 1797
Size: 1496 301
DraI TTT'AAA
Cuts at: 0 935 1797
Size: 935 862
EaeI y'GGCC_r
Cuts at: 0 1115 1797
Size: 1115 682
EarI CTCTTCn'nnn_
Cuts at: 0 110 1419 1797
Size: 110 1309 378
Fragments arranged by size:
1309 378 110
EcoICRI GAG'CTC
Cuts at: 0 1253 1778 1797
Size: 1253 525 19
Fragments arranged by size:
1253 525 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1358 1797
Size: 1358 439
EcoO109I rG'GnC_Cy
Cuts at: 0 1106 1797
Size: 1106 691
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 511 808 1797
Size: 511 297 989
Fragments arranged by size:
989 511 297
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1179 1211 1454 1486 1546 1578 1797
Size: 1179 32 243 32 60 32 219
Fragments arranged by size:
1179 243 219 60 32 32 32
KpnI G_GTAC'C
Cuts at: 0 255 1797
Size: 255 1542
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 1074 1797
Size: 656 418 723
Fragments arranged by size:
723 656 418
MslI CAynn'nnrTG
Cuts at: 0 657 1678 1797
Size: 657 1021 119
Fragments arranged by size:
1021 657 119
MspA1I CmG'CkG
Cuts at: 0 536 562 1797
Size: 536 26 1235
Fragments arranged by size:
1235 536 26
NdeI CA'TA_TG
Cuts at: 0 654 1797
Size: 654 1143
NsiI A_TGCA'T
Cuts at: 0 459 1797
Size: 459 1338
NspI r_CATG'y
Cuts at: 0 1677 1797
Size: 1677 120
PacI TTA_AT'TAA
Cuts at: 0 1535 1797
Size: 1535 262
PasI CC'CwG_GG
Cuts at: 0 346 1797
Size: 346 1451
PfoI T'CCnGG_A
Cuts at: 0 1374 1797
Size: 1374 423
PmlI CAC'GTG
Cuts at: 0 1703 1797
Size: 1703 94
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 695 727 1797
Size: 695 32 1070
Fragments arranged by size:
1070 695 32
PpuMI rG'GwC_Cy
Cuts at: 0 1106 1797
Size: 1106 691
PvuII CAG'CTG
Cuts at: 0 536 1797
Size: 536 1261
SacI G_AGCT'C
Cuts at: 0 1255 1780 1797
Size: 1255 525 17
Fragments arranged by size:
1255 525 17
SanDI GG'GwC_CC
Cuts at: 0 1106 1797
Size: 1106 691
SexAI A'CCwGG_T
Cuts at: 0 568 1797
Size: 568 1229
SfcI C'TryA_G
Cuts at: 0 1143 1797
Size: 1143 654
SmlI C'TyrA_G
Cuts at: 0 1440 1797
Size: 1440 357
SspI AAT'ATT
Cuts at: 0 63 1433 1797
Size: 63 1370 364
Fragments arranged by size:
1370 364 63
StyI C'CwwG_G
Cuts at: 0 1502 1797
Size: 1502 295
TatI w'GTAC_w
Cuts at: 0 1082 1797
Size: 1082 715
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 566 1024 1107 1797
Size: 566 458 83 690
Fragments arranged by size:
690 566 458 83
Enzymes that do cut:
AarI AccI Acc65I AlwNI ApoI BaeI BamHI BanI
BanII BbsI BcgI BciVI BclI BfrBI BglI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaWI BseRI
BseYI BsiHKAI Bsp1286I BspMI BsrGI BssSI BstBI BstEII
BstYI Bsu36I BtsI DraI EaeI EarI EcoICRI Eco57MI
EcoO109I FalI Hin4I KpnI MmeI MslI MspA1I NdeI
NsiI NspI PacI PasI PfoI PmlI PpiI PpuMI
PvuII SacI SanDI SexAI SfcI SmlI SspI StyI
TatI XcmI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BbeI BbvCI BglII BlpI BmgBI BmtI
BsaI BsaBI BsaHI BsaXI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII BstAPI
BstXI BstZ17I BtgI BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MfeI MluI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI PciI
PflMI PmeI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI
XmnI ZraI