Restriction map for
pFN21AE2021
(Linear) (Six-base) MAPSORT of: KIEE2021.seq Check: 4346 from: 1 to: 1797
KIEE2021.orf
With 182 enzymes: SgfI *
July 3, 2012 17:07 ..
AccI GT'mk_AC
Cuts at: 0 298 1797
Size: 298 1499
AclI AA'CG_TT
Cuts at: 0 1295 1797
Size: 1295 502
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 828 1797
Size: 545 283 969
Fragments arranged by size:
969 545 283
AfeI AGC'GCT
Cuts at: 0 326 1797
Size: 326 1471
AflII C'TTAA_G
Cuts at: 0 1132 1797
Size: 1132 665
AhdI GACnn_n'nnGTC
Cuts at: 0 1629 1797
Size: 1629 168
AleI CACnn'nnGTG
Cuts at: 0 743 1797
Size: 743 1054
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1768 1797
Size: 1768 29
ApaI G_GGCC'C
Cuts at: 0 1074 1797
Size: 1074 723
ApaLI G'TGCA_C
Cuts at: 0 736 1797
Size: 736 1061
ApoI r'AATT_y
Cuts at: 0 363 1797
Size: 363 1434
AvaI C'yCGr_G
Cuts at: 0 1067 1797
Size: 1067 730
AvrII C'CTAG_G
Cuts at: 0 1542 1797
Size: 1542 255
BamHI G'GATC_C
Cuts at: 0 41 482 900 1538 1797
Size: 41 441 418 638 259
Fragments arranged by size:
638 441 418 259 41
BanII G_rGCy'C
Cuts at: 0 1074 1797
Size: 1074 723
BbsI GAAGACnn'nnnn_
Cuts at: 0 23 385 1364 1797
Size: 23 362 979 433
Fragments arranged by size:
979 433 362 23
BbvCI CC'TCA_GC
Cuts at: 0 166 586 1495 1797
Size: 166 420 909 302
Fragments arranged by size:
909 420 302 166
BclI T'GATC_A
Cuts at: 0 1005 1797
Size: 1005 792
BglI GCCn_nnn'nGGC
Cuts at: 0 1765 1797
Size: 1765 32
BglII A'GATC_T
Cuts at: 0 834 1797
Size: 834 963
Bme1580I G_kGCm'C
Cuts at: 0 598 740 1074 1797
Size: 598 142 334 723
Fragments arranged by size:
723 598 334 142
BmrI ACTGGGnnnn_n'
Cuts at: 0 1357 1755 1797
Size: 1357 398 42
Fragments arranged by size:
1357 398 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 228 1797
Size: 228 1569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 872 1756 1797
Size: 872 884 41
Fragments arranged by size:
884 872 41
Bpu10I CC'TnA_GC
Cuts at: 0 166 586 1495 1797
Size: 166 420 909 302
Fragments arranged by size:
909 420 302 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 738 859 1797
Size: 738 121 938
Fragments arranged by size:
938 738 121
BsaI GGTCTCn'nnnn_
Cuts at: 0 342 1797
Size: 342 1455
BsaAI yAC'GTr
Cuts at: 0 120 1797
Size: 120 1677
BsaBI GATnn'nnATC
Cuts at: 0 905 1797
Size: 905 892
BsaHI Gr'CG_yC
Cuts at: 0 14 1797
Size: 14 1783
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1240 1270 1768 1797
Size: 1240 30 498 29
Fragments arranged by size:
1240 498 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 1678 1705 1797
Size: 154 1524 27 92
Fragments arranged by size:
1524 154 92 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 1797
Size: 130 1667
BsiEI CG_ry'CG
Cuts at: 0 137 1797
Size: 137 1660
BsiHKAI G_wGCw'C
Cuts at: 0 740 1797
Size: 740 1057
BsmI GAATG_Cn'
Cuts at: 0 288 1797
Size: 288 1509
Bsp1286I G_dGCh'C
Cuts at: 0 598 740 1074 1797
Size: 598 142 334 723
Fragments arranged by size:
723 598 334 142
BspHI T'CATG_A
Cuts at: 0 264 1797
Size: 264 1533
BsrDI GCAATG_nn'
Cuts at: 0 464 1283 1589 1797
Size: 464 819 306 208
Fragments arranged by size:
819 464 306 208
BsrFI r'CCGG_y
Cuts at: 0 50 1797
Size: 50 1747
BsrGI T'GTAC_A
Cuts at: 0 533 1797
Size: 533 1264
BssSI C'ACGA_G
Cuts at: 0 947 1797
Size: 947 850
BstYI r'GATC_y
Cuts at: 0 41 482 834 900 1538 1797
Size: 41 441 352 66 638 259
Fragments arranged by size:
638 441 352 259 66 41
BstZ17I GTA'TAC
Cuts at: 0 299 1797
Size: 299 1498
Bsu36I CC'TnA_GG
Cuts at: 0 37 1797
Size: 37 1760
BtgI C'CryG_G
Cuts at: 0 83 137 302 1021 1673 1797
Size: 83 54 165 719 652 124
Fragments arranged by size:
719 652 165 124 83 54
BtsI GCAGTG_nn'
Cuts at: 0 598 1786 1797
Size: 598 1188 11
Fragments arranged by size:
1188 598 11
EaeI y'GGCC_r
Cuts at: 0 134 1797
Size: 134 1663
EagI C'GGCC_G
Cuts at: 0 134 1797
Size: 134 1663
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 828 872 1756 1797
Size: 545 283 44 884 41
Fragments arranged by size:
884 545 283 44 41
EcoO109I rG'GnC_Cy
Cuts at: 0 1071 1731 1797
Size: 1071 660 66
Fragments arranged by size:
1071 660 66
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 353 1797
Size: 353 1444
HaeII r_GCGC'y
Cuts at: 0 328 558 1797
Size: 328 230 1239
Fragments arranged by size:
1239 328 230
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 643 675 691 723 775 807 885 917
Size: 643 32 16 32 52 32 78 32
Cuts at: 917 1615 1647 1797
Size: 698 32 150
Fragments arranged by size:
698 643 150 78 52 32 32 32
32 32 16
HindIII A'AGCT_T
Cuts at: 0 825 1797
Size: 825 972
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 878 1283 1797
Size: 176 702 405 514
Fragments arranged by size:
702 514 405 176
MslI CAynn'nnrTG
Cuts at: 0 667 743 1797
Size: 667 76 1054
Fragments arranged by size:
1054 667 76
MspA1I CmG'CkG
Cuts at: 0 78 85 139 1101 1797
Size: 78 7 54 962 696
Fragments arranged by size:
962 696 78 54 7
NcoI C'CATG_G
Cuts at: 0 302 1797
Size: 302 1495
NotI GC'GGCC_GC
Cuts at: 0 134 1797
Size: 134 1663
NspI r_CATG'y
Cuts at: 0 1015 1794 1797
Size: 1015 779 3
Fragments arranged by size:
1015 779 3
PflMI CCAn_nnn'nTGG
Cuts at: 0 237 1797
Size: 237 1560
PmlI CAC'GTG
Cuts at: 0 120 1797
Size: 120 1677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1768 1797
Size: 1768 29
PpuMI rG'GwC_Cy
Cuts at: 0 1731 1797
Size: 1731 66
PspOMI G'GGCC_C
Cuts at: 0 1070 1797
Size: 1070 727
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 759 791 1797
Size: 759 32 1006
Fragments arranged by size:
1006 759 32
PvuII CAG'CTG
Cuts at: 0 1101 1797
Size: 1101 696
SacII CC_GC'GG
Cuts at: 0 86 140 1797
Size: 86 54 1657
Fragments arranged by size:
1657 86 54
SfcI C'TryA_G
Cuts at: 0 1050 1797
Size: 1050 747
SmaI CCC'GGG
Cuts at: 0 1069 1797
Size: 1069 728
SmlI C'TyrA_G
Cuts at: 0 753 874 1132 1797
Size: 753 121 258 665
Fragments arranged by size:
753 665 258 121
SphI G_CATG'C
Cuts at: 0 1015 1794 1797
Size: 1015 779 3
Fragments arranged by size:
1015 779 3
StyI C'CwwG_G
Cuts at: 0 302 978 1088 1542 1797
Size: 302 676 110 454 255
Fragments arranged by size:
676 454 302 255 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1014 1797
Size: 1014 783
TatI w'GTAC_w
Cuts at: 0 533 839 1797
Size: 533 306 958
Fragments arranged by size:
958 533 306
Tth111I GACn'n_nGTC
Cuts at: 0 1525 1797
Size: 1525 272
XbaI T'CTAG_A
Cuts at: 0 1635 1797
Size: 1635 162
XmaI C'CCGG_G
Cuts at: 0 1067 1797
Size: 1067 730
Enzymes that do cut:
AccI AclI AcuI AfeI AflII AhdI AleI AloI
ApaI ApaLI ApoI AvaI AvrII BamHI BanII BbsI
BbvCI BclI BglI BglII Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI
BsrGI BssSI BstYI BstZ17I Bsu36I BtgI BtsI EaeI
EagI Eco57MI EcoO109I FalI HaeII Hin4I HindIII MmeI
MslI MspA1I NcoI NotI NspI PflMI PmlI PpiI
PpuMI PspOMI PsrI PvuII SacII SfcI SmaI SmlI
SphI StyI TaqII TatI Tth111I XbaI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AflIII AgeI AlfI AlwNI AscI
AseI AsiSI BaeI BanI BbeI BcgI BciVI BfrBI
BlpI BmgBI BmtI BsaWI BseYI BsiWI BsmBI BspEI
BspMI BsrBI BssHII BstAPI BstBI BstEII BstXI BtgZI
ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NruI NsiI PacI PasI PciI PfoI
PmeI PshAI PsiI PspXI PstI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
TstI XcmI XhoI XmnI ZraI