Restriction map for
pFN21AE2018
(Linear) (Six-base) MAPSORT of: KIEE2018.seq Check: 399 from: 1 to: 1782
KIEE2018.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AccI GT'mk_AC
Cuts at: 0 442 1050 1753 1782
Size: 442 608 703 29
Fragments arranged by size:
703 608 442 29
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 361 707 1147 1781 1782
Size: 1 360 346 440 634 1
Fragments arranged by size:
634 440 360 346 1 1
AflII C'TTAA_G
Cuts at: 0 918 1663 1782
Size: 918 745 119
Fragments arranged by size:
918 745 119
AflIII A'CryG_T
Cuts at: 0 1286 1303 1782
Size: 1286 17 479
Fragments arranged by size:
1286 479 17
AleI CACnn'nnGTG
Cuts at: 0 1241 1782
Size: 1241 541
ApoI r'AATT_y
Cuts at: 0 628 817 1425 1782
Size: 628 189 608 357
Fragments arranged by size:
628 608 357 189
AvaI C'yCGr_G
Cuts at: 0 1718 1782
Size: 1718 64
BanII G_rGCy'C
Cuts at: 0 338 1782
Size: 338 1444
BbsI GAAGACnn'nnnn_
Cuts at: 0 1046 1782
Size: 1046 736
BclI T'GATC_A
Cuts at: 0 900 1782
Size: 900 882
BfrBI ATG'CAT
Cuts at: 0 107 1782
Size: 107 1675
BglII A'GATC_T
Cuts at: 0 1602 1782
Size: 1602 180
Bme1580I G_kGCm'C
Cuts at: 0 122 956 1782
Size: 122 834 826
Fragments arranged by size:
834 826 122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1036 1782
Size: 1036 746
BsaAI yAC'GTr
Cuts at: 0 1306 1782
Size: 1306 476
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1349 1379 1782
Size: 1349 30 403
Fragments arranged by size:
1349 403 30
BseYI C'CCAG_C
Cuts at: 0 94 1782
Size: 94 1688
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1397 1782
Size: 1397 385
BsmI GAATG_Cn'
Cuts at: 0 1674 1782
Size: 1674 108
Bsp1286I G_dGCh'C
Cuts at: 0 122 338 956 1782
Size: 122 216 618 826
Fragments arranged by size:
826 618 216 122
BsrGI T'GTAC_A
Cuts at: 0 882 1782
Size: 882 900
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1005 1782
Size: 1005 777
BstYI r'GATC_y
Cuts at: 0 1602 1634 1782
Size: 1602 32 148
Fragments arranged by size:
1602 148 32
Bsu36I CC'TnA_GG
Cuts at: 0 1094 1782
Size: 1094 688
DraI TTT'AAA
Cuts at: 0 63 1782
Size: 63 1719
EarI CTCTTCn'nnn_
Cuts at: 0 44 62 326 470 977 1625 1782
Size: 44 18 264 144 507 648 157
Fragments arranged by size:
648 507 264 157 144 44 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 361 707 1036 1147 1781 1782
Size: 1 360 346 329 111 634 1
Fragments arranged by size:
634 360 346 329 111 1 1
EcoO109I rG'GnC_Cy
Cuts at: 0 1668 1782
Size: 1668 114
EcoRV GAT'ATC
Cuts at: 0 353 1782
Size: 353 1429
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 58 765 797 1619 1651 1782
Size: 26 32 707 32 822 32 131
Fragments arranged by size:
822 707 131 32 32 32 26
HindIII A'AGCT_T
Cuts at: 0 642 1782
Size: 642 1140
MfeI C'AATT_G
Cuts at: 0 425 749 1782
Size: 425 324 1033
Fragments arranged by size:
1033 425 324
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1163 1782
Size: 1163 619
MslI CAynn'nnrTG
Cuts at: 0 1241 1782
Size: 1241 541
MspA1I CmG'CkG
Cuts at: 0 1167 1782
Size: 1167 615
NsiI A_TGCA'T
Cuts at: 0 109 1782
Size: 109 1673
NspI r_CATG'y
Cuts at: 0 1290 1782
Size: 1290 492
PciI A'CATG_T
Cuts at: 0 1286 1782
Size: 1286 496
PpuMI rG'GwC_Cy
Cuts at: 0 1668 1782
Size: 1668 114
PshAI GACnn'nnGTC
Cuts at: 0 1046 1782
Size: 1046 736
PspXI vC'TCGA_Gb
Cuts at: 0 1718 1782
Size: 1718 64
PvuII CAG'CTG
Cuts at: 0 1167 1782
Size: 1167 615
SapI GCTCTTCn'nnn_
Cuts at: 0 44 326 1782
Size: 44 282 1456
Fragments arranged by size:
1456 282 44
ScaI AGT'ACT
Cuts at: 0 1063 1782
Size: 1063 719
SfcI C'TryA_G
Cuts at: 0 5 1782
Size: 5 1777
SmlI C'TyrA_G
Cuts at: 0 918 1663 1718 1782
Size: 918 745 55 64
Fragments arranged by size:
918 745 64 55
SspI AAT'ATT
Cuts at: 0 1500 1770 1782
Size: 1500 270 12
Fragments arranged by size:
1500 270 12
StuI AGG'CCT
Cuts at: 0 535 1782
Size: 535 1247
TatI w'GTAC_w
Cuts at: 0 882 1061 1782
Size: 882 179 721
Fragments arranged by size:
882 721 179
XhoI C'TCGA_G
Cuts at: 0 1718 1782
Size: 1718 64
XmnI GAAnn'nnTTC
Cuts at: 0 143 1510 1709 1782
Size: 143 1367 199 73
Fragments arranged by size:
1367 199 143 73
Enzymes that do cut:
AccI AcuI AflII AflIII AleI ApoI AvaI BanII
BbsI BclI BfrBI BglII Bme1580I BpmI BsaAI BsaXI
BseYI BsgI BsmI Bsp1286I BsrGI BstXI BstYI Bsu36I
DraI EarI Eco57MI EcoO109I EcoRV Hin4I HindIII MfeI
MmeI MslI MspA1I NsiI NspI PciI PpuMI PshAI
PspXI PvuII SapI ScaI SfcI SmlI SspI StuI
TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BglI
BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRI FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PflMI PfoI
PmeI PmlI PpiI PsiI PspOMI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StyI SwaI TaqII TstI Tth111I XbaI XcmI XmaI
ZraI