Restriction map for
pFN21AE2016
(Linear) (Six-base) MAPSORT of: KIEE2016.seq Check: 2025 from: 1 to: 1779
KIEE2016.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 215 1779
Size: 215 1564
AatII G_ACGT'C
Cuts at: 0 317 335 1779
Size: 317 18 1444
Fragments arranged by size:
1444 317 18
AccI GT'mk_AC
Cuts at: 0 41 1779
Size: 41 1738
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1060 1240 1417 1779
Size: 1060 180 177 362
Fragments arranged by size:
1060 362 180 177
AflIII A'CryG_T
Cuts at: 0 825 1153 1779
Size: 825 328 626
Fragments arranged by size:
825 626 328
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 317 1779
Size: 317 1462
AlwNI CAG_nnn'CTG
Cuts at: 0 447 1038 1218 1530 1567 1779
Size: 447 591 180 312 37 212
Fragments arranged by size:
591 447 312 212 180 37
ApaI G_GGCC'C
Cuts at: 0 235 911 1737 1779
Size: 235 676 826 42
Fragments arranged by size:
826 676 235 42
ApoI r'AATT_y
Cuts at: 0 844 1779
Size: 844 935
AvaI C'yCGr_G
Cuts at: 0 29 1442 1779
Size: 29 1413 337
Fragments arranged by size:
1413 337 29
AvrII C'CTAG_G
Cuts at: 0 1362 1779
Size: 1362 417
BanI G'GyrC_C
Cuts at: 0 34 307 1779
Size: 34 273 1472
Fragments arranged by size:
1472 273 34
BanII G_rGCy'C
Cuts at: 0 235 581 911 1148 1204 1737 1779
Size: 235 346 330 237 56 533 42
Fragments arranged by size:
533 346 330 237 235 56 42
BbeI G_GCGC'C
Cuts at: 0 38 311 1779
Size: 38 273 1468
Fragments arranged by size:
1468 273 38
BbvCI CC'TCA_GC
Cuts at: 0 1469 1779
Size: 1469 310
BglI GCCn_nnn'nGGC
Cuts at: 0 230 1779
Size: 230 1549
BglII A'GATC_T
Cuts at: 0 774 1779
Size: 774 1005
BlpI GC'TnA_GC
Cuts at: 0 239 1211 1681 1779
Size: 239 972 470 98
Fragments arranged by size:
972 470 239 98
Bme1580I G_kGCm'C
Cuts at: 0 235 572 911 1737 1779
Size: 235 337 339 826 42
Fragments arranged by size:
826 339 337 235 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 240 1779
Size: 240 1539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 952 1027 1071 1779
Size: 247 705 75 44 708
Fragments arranged by size:
708 705 247 75 44
Bpu10I CC'TnA_GC
Cuts at: 0 800 1469 1779
Size: 800 669 310
Fragments arranged by size:
800 669 310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1015 1779
Size: 1015 764
BsaI GGTCTCn'nnnn_
Cuts at: 0 112 638 949 1779
Size: 112 526 311 830
Fragments arranged by size:
830 526 311 112
BsaAI yAC'GTr
Cuts at: 0 1674 1779
Size: 1674 105
BsaBI GATnn'nnATC
Cuts at: 0 976 1779
Size: 976 803
BsaHI Gr'CG_yC
Cuts at: 0 35 124 308 314 332 1779
Size: 35 89 184 6 18 1447
Fragments arranged by size:
1447 184 89 35 18 6
BsaWI w'CCGG_w
Cuts at: 0 98 1779
Size: 98 1681
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1130 1160 1779
Size: 1130 30 619
Fragments arranged by size:
1130 619 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 967 1074 1462 1779
Size: 967 107 388 317
Fragments arranged by size:
967 388 317 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 679 1779
Size: 280 399 1100
Fragments arranged by size:
1100 399 280
BsiEI CG_ry'CG
Cuts at: 0 103 1779
Size: 103 1676
BsiHKAI G_wGCw'C
Cuts at: 0 24 370 1148 1204 1682 1779
Size: 24 346 778 56 478 97
Fragments arranged by size:
778 478 346 97 56 24
BsmI GAATG_Cn'
Cuts at: 0 1751 1779
Size: 1751 28
Bsp1286I G_dGCh'C
Cuts at: 0 24 235 370 572 581 911 1148 1204
Size: 24 211 135 202 9 330 237 56
Cuts at: 1204 1682 1737 1779
Size: 478 55 42
Fragments arranged by size:
478 330 237 211 202 135 56 55
42 24 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 215 1124 1779
Size: 215 909 655
Fragments arranged by size:
909 655 215
BsrBI CCG'CTC
Cuts at: 0 1646 1779
Size: 1646 133
BsrFI r'CCGG_y
Cuts at: 0 645 1516 1625 1779
Size: 645 871 109 154
Fragments arranged by size:
871 645 154 109
BstAPI GCAn_nnn'nTGC
Cuts at: 0 486 648 1779
Size: 486 162 1131
Fragments arranged by size:
1131 486 162
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1339 1779
Size: 1339 440
BstYI r'GATC_y
Cuts at: 0 774 1028 1779
Size: 774 254 751
Fragments arranged by size:
774 751 254
BtgI C'CryG_G
Cuts at: 0 572 623 663 1613 1779
Size: 572 51 40 950 166
Fragments arranged by size:
950 572 166 51 40
DraIII CAC_nnn'GTG
Cuts at: 0 711 1677 1691 1779
Size: 711 966 14 88
Fragments arranged by size:
966 711 88 14
EaeI y'GGCC_r
Cuts at: 0 89 626 974 1420 1779
Size: 89 537 348 446 359
Fragments arranged by size:
537 446 359 348 89
EarI CTCTTCn'nnn_
Cuts at: 0 778 1061 1192 1779
Size: 778 283 131 587
Fragments arranged by size:
778 587 283 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 979 1084 1779
Size: 979 105 695
Fragments arranged by size:
979 695 105
EcoICRI GAG'CTC
Cuts at: 0 1146 1202 1779
Size: 1146 56 577
Fragments arranged by size:
1146 577 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 952 1027 1060 1071 1240 1417 1779
Size: 247 705 75 33 11 169 177 362
Fragments arranged by size:
705 362 247 177 169 75 33 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 739 1779
Size: 739 1040
EcoO109I rG'GnC_Cy
Cuts at: 0 48 231 1044 1224 1359 1734 1770 1779
Size: 48 183 813 180 135 375 36 9
Fragments arranged by size:
813 375 183 180 135 48 36 9
EcoRI G'AATT_C
Cuts at: 0 844 1779
Size: 844 935
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 387 885 1779
Size: 387 498 894
Fragments arranged by size:
894 498 387
HaeII r_GCGC'y
Cuts at: 0 38 311 671 1779
Size: 38 273 360 1108
Fragments arranged by size:
1108 360 273 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 23 55 1130 1162 1779
Size: 23 32 1075 32 617
Fragments arranged by size:
1075 617 32 32 23
HincII GTy'rAC
Cuts at: 0 431 1779
Size: 431 1348
KasI G'GCGC_C
Cuts at: 0 34 307 1779
Size: 34 273 1472
Fragments arranged by size:
1472 273 34
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 834 1779
Size: 834 945
MscI TGG'CCA
Cuts at: 0 976 1422 1779
Size: 976 446 357
Fragments arranged by size:
976 446 357
MslI CAynn'nnrTG
Cuts at: 0 299 839 1337 1779
Size: 299 540 498 442
Fragments arranged by size:
540 498 442 299
MspA1I CmG'CkG
Cuts at: 0 129 503 512 913 1035 1395 1501 1615
Size: 129 374 9 401 122 360 106 114
Cuts at: 1615 1779
Size: 164
Fragments arranged by size:
401 374 360 164 129 122 114 106
9
NaeI GCC'GGC
Cuts at: 0 1518 1627 1779
Size: 1518 109 152
Fragments arranged by size:
1518 152 109
NarI GG'CG_CC
Cuts at: 0 35 308 1779
Size: 35 273 1471
Fragments arranged by size:
1471 273 35
NdeI CA'TA_TG
Cuts at: 0 1131 1779
Size: 1131 648
NgoMIV G'CCGG_C
Cuts at: 0 1516 1625 1779
Size: 1516 109 154
Fragments arranged by size:
1516 154 109
NspI r_CATG'y
Cuts at: 0 1157 1779
Size: 1157 622
PciI A'CATG_T
Cuts at: 0 1153 1779
Size: 1153 626
PflMI CCAn_nnn'nTGG
Cuts at: 0 447 1302 1779
Size: 447 855 477
Fragments arranged by size:
855 477 447
PmlI CAC'GTG
Cuts at: 0 1674 1779
Size: 1674 105
PpuMI rG'GwC_Cy
Cuts at: 0 1044 1359 1779
Size: 1044 315 420
Fragments arranged by size:
1044 420 315
PshAI GACnn'nnGTC
Cuts at: 0 1552 1779
Size: 1552 227
PspOMI G'GGCC_C
Cuts at: 0 231 907 1733 1779
Size: 231 676 826 46
Fragments arranged by size:
826 676 231 46
PstI C_TGCA'G
Cuts at: 0 212 1634 1779
Size: 212 1422 145
Fragments arranged by size:
1422 212 145
PvuII CAG'CTG
Cuts at: 0 503 512 1035 1395 1779
Size: 503 9 523 360 384
Fragments arranged by size:
523 503 384 360 9
SacI G_AGCT'C
Cuts at: 0 1148 1204 1779
Size: 1148 56 575
Fragments arranged by size:
1148 575 56
SacII CC_GC'GG
Cuts at: 0 1616 1779
Size: 1616 163
SapI GCTCTTCn'nnn_
Cuts at: 0 778 1192 1779
Size: 778 414 587
Fragments arranged by size:
778 587 414
SexAI A'CCwGG_T
Cuts at: 0 1529 1779
Size: 1529 250
SfcI C'TryA_G
Cuts at: 0 208 1630 1779
Size: 208 1422 149
Fragments arranged by size:
1422 208 149
SfoI GGC'GCC
Cuts at: 0 36 309 1779
Size: 36 273 1470
Fragments arranged by size:
1470 273 36
SmlI C'TyrA_G
Cuts at: 0 994 1779
Size: 994 785
StuI AGG'CCT
Cuts at: 0 1312 1779
Size: 1312 467
StyI C'CwwG_G
Cuts at: 0 10 1362 1779
Size: 10 1352 417
Fragments arranged by size:
1352 417 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1723 1779
Size: 1723 56
TatI w'GTAC_w
Cuts at: 0 461 1779
Size: 461 1318
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1402 1434 1466 1498 1779
Size: 1402 32 32 32 281
Fragments arranged by size:
1402 281 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1075 1779
Size: 1075 704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 448 1779
Size: 448 1331
ZraI GAC'GTC
Cuts at: 0 315 333 1779
Size: 315 18 1446
Fragments arranged by size:
1446 315 18
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlfI AlwNI ApaI
ApoI AvaI AvrII BanI BanII BbeI BbvCI BglI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI
BstYI BtgI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PflMI PmlI PpuMI PshAI PspOMI PstI
PvuII SacI SacII SapI SexAI SfcI SfoI SmlI
StuI StyI TaqII TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AloI
ApaLI AscI AseI AsiSI BaeI BamHI BbsI BcgI
BciVI BclI BfrBI BmgBI BmtI BplI BseYI BsiWI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EagI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI NcoI NheI NotI NruI NsiI
PacI PasI PfoI PmeI PpiI PsiI PspXI PsrI
PvuI RsrII SalI SanDI SbfI ScaI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
XbaI XhoI XmaI XmnI