Restriction map for
pFN21AE2015
(Linear) (Six-base) MAPSORT of: KIEE2015.seq Check: 3829 from: 1 to: 1776
KIEE2015.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AccI GT'mk_AC
Cuts at: 0 736 1776
Size: 736 1040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 1776
Size: 866 910
AhdI GACnn_n'nnGTC
Cuts at: 0 354 1776
Size: 354 1422
AlwNI CAG_nnn'CTG
Cuts at: 0 670 682 1776
Size: 670 12 1094
Fragments arranged by size:
1094 670 12
ApoI r'AATT_y
Cuts at: 0 630 919 967 1119 1776
Size: 630 289 48 152 657
Fragments arranged by size:
657 630 289 152 48
BanII G_rGCy'C
Cuts at: 0 229 1776
Size: 229 1547
BbsI GAAGACnn'nnnn_
Cuts at: 0 799 966 1776
Size: 799 167 810
Fragments arranged by size:
810 799 167
BclI T'GATC_A
Cuts at: 0 366 405 1755 1776
Size: 366 39 1350 21
Fragments arranged by size:
1350 366 39 21
BglI GCCn_nnn'nGGC
Cuts at: 0 1586 1776
Size: 1586 190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 1776
Size: 144 1632
Bpu10I CC'TnA_GC
Cuts at: 0 887 1776
Size: 887 889
BsaI GGTCTCn'nnnn_
Cuts at: 0 1046 1232 1412 1457 1481 1592 1622 1673
Size: 1046 186 180 45 24 111 30 51
Cuts at: 1673 1697 1776
Size: 24 79
Fragments arranged by size:
1046 186 180 111 79 51 45 30
24 24
BsaBI GATnn'nnATC
Cuts at: 0 371 1776
Size: 371 1405
BsaWI w'CCGG_w
Cuts at: 0 796 1776
Size: 796 980
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 718 748 1776
Size: 718 30 1028
Fragments arranged by size:
1028 718 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 560 992 1187 1415 1430 1439 1454 1475
Size: 560 432 195 228 15 9 15 21
Cuts at: 1475 1508 1520 1529 1541 1550 1562 1571 1583
Size: 33 12 9 12 9 12 9 12
Cuts at: 1583 1610 1664 1691 1715 1776
Size: 27 54 27 24 61
Fragments arranged by size:
560 432 228 195 61 54 33 27
27 24 21 15 15 12 12 12
12 9 9 9 9
BsiHKAI G_wGCw'C
Cuts at: 0 229 306 1776
Size: 229 77 1470
Fragments arranged by size:
1470 229 77
Bsp1286I G_dGCh'C
Cuts at: 0 229 306 1776
Size: 229 77 1470
Fragments arranged by size:
1470 229 77
BspHI T'CATG_A
Cuts at: 0 971 1776
Size: 971 805
BsrDI GCAATG_nn'
Cuts at: 0 1143 1776
Size: 1143 633
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1133 1776
Size: 1133 643
BstEII G'GTnAC_C
Cuts at: 0 610 1776
Size: 610 1166
BstXI CCAn_nnnn'nTGG
Cuts at: 0 82 1776
Size: 82 1694
Bsu36I CC'TnA_GG
Cuts at: 0 1167 1776
Size: 1167 609
BtgI C'CryG_G
Cuts at: 0 615 924 1008 1602 1776
Size: 615 309 84 594 174
Fragments arranged by size:
615 594 309 174 84
BtsI GCAGTG_nn'
Cuts at: 0 1620 1776
Size: 1620 156
DraI TTT'AAA
Cuts at: 0 762 1776
Size: 762 1014
EarI CTCTTCn'nnn_
Cuts at: 0 294 576 1776
Size: 294 282 1200
Fragments arranged by size:
1200 294 282
EcoICRI GAG'CTC
Cuts at: 0 227 1776
Size: 227 1549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 866 1776
Size: 144 722 910
Fragments arranged by size:
910 722 144
EcoNI CCTnn'n_nnAGG
Cuts at: 0 593 1776
Size: 593 1183
EcoO109I rG'GnC_Cy
Cuts at: 0 586 1776
Size: 586 1190
EcoRI G'AATT_C
Cuts at: 0 919 967 1119 1776
Size: 919 48 152 657
Fragments arranged by size:
919 657 152 48
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 323 1776
Size: 323 1453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 379 411 664 696 718 750
Size: 340 32 7 32 253 32 22 32
Cuts at: 750 1776
Size: 1026
Fragments arranged by size:
1026 340 253 32 32 32 32 22
7
HincII GTy'rAC
Cuts at: 0 334 1776
Size: 334 1442
MslI CAynn'nnrTG
Cuts at: 0 940 1776
Size: 940 836
MspA1I CmG'CkG
Cuts at: 0 152 617 1298 1313 1776
Size: 152 465 681 15 463
Fragments arranged by size:
681 465 463 152 15
NcoI C'CATG_G
Cuts at: 0 924 1776
Size: 924 852
NdeI CA'TA_TG
Cuts at: 0 345 384 1776
Size: 345 39 1392
Fragments arranged by size:
1392 345 39
PflMI CCAn_nnn'nTGG
Cuts at: 0 328 902 1776
Size: 328 574 874
Fragments arranged by size:
874 574 328
PpuMI rG'GwC_Cy
Cuts at: 0 586 1776
Size: 586 1190
PshAI GACnn'nnGTC
Cuts at: 0 329 1160 1776
Size: 329 831 616
Fragments arranged by size:
831 616 329
SacI G_AGCT'C
Cuts at: 0 229 1776
Size: 229 1547
SacII CC_GC'GG
Cuts at: 0 618 1776
Size: 618 1158
SapI GCTCTTCn'nnn_
Cuts at: 0 294 1776
Size: 294 1482
SfcI C'TryA_G
Cuts at: 0 147 423 465 737 1212 1308 1746 1776
Size: 147 276 42 272 475 96 438 30
Fragments arranged by size:
475 438 276 272 147 96 42 30
StyI C'CwwG_G
Cuts at: 0 75 291 924 1776
Size: 75 216 633 852
Fragments arranged by size:
852 633 216 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1256 1436 1481 1505 1646 1697 1721 1776
Size: 1256 180 45 24 141 51 24 55
Fragments arranged by size:
1256 180 141 55 51 45 24 24
Tth111I GACn'n_nGTC
Cuts at: 0 173 1776
Size: 173 1603
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 325 1776
Size: 325 1451
XmnI GAAnn'nnTTC
Cuts at: 0 1119 1776
Size: 1119 657
Enzymes that do cut:
AccI AcuI AhdI AlwNI ApoI BanII BbsI BclI
BglI BpmI Bpu10I BsaI BsaBI BsaWI BsaXI BseRI
BsiHKAI Bsp1286I BspHI BsrDI BstAPI BstEII BstXI Bsu36I
BtgI BtsI DraI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI Hin4I HincII MslI MspA1I NcoI NdeI
PflMI PpuMI PshAI SacI SacII SapI SfcI StyI
TaqII Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpuEI BsaAI BsaHI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstYI BstZ17I BtgZI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoRV FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI XbaI XhoI XmaI ZraI